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We narrowed to 578 results for: SPY

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Showing: 201 - 220 of 578 results

  3. pcDNA3.1-H2-18 Heavy chain-SpyTag003

    Plasmid
    #216307
    Purpose
    Expresses anti-HER2 H2-18 Heavy chain-SpyTag003 in mammalian cells. To be paired with H2-18 light chain to form the anti-HER2 H2-18 Fab
    Depositor
    Mark Howarth
    Article
    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:
    Insert
    H2-18 Heavy chain-SpyTag003
    Use
    Affinity Reagent/ Antibody
    Tags
    His6 tag, Signal peptide, and SpyTag003
    Expression
    Mammalian
    Promoter
    T7 promoter
    Available Since
    June 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    216307-plasmid-map-sequence-id-433370

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  6. pET21b-MBPx-MBPx-SpyTag003

    Plasmid
    #216291
    Purpose
    Expresses tandem MBPx linked to-SpyTag003 in bacterial cells. MBPx is a variant of MBP (A312V I317V Δ172-173 Δ175-176) with enhanced affinity for maltose.
    Depositor
    Mark Howarth
    Article
    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:
    Insert
    MBPx-MBPx-SpyTag003
    Use
    Affinity Reagent/ Antibody
    Tags
    His6 and SpyTag003
    Expression
    Bacterial
    Promoter
    T7 promoter
    Available Since
    Oct. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    216291-plasmid-map-sequence-id-443903

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  8. pEC3111 (pSpy1C-Plac(3)_ffluc)

    Plasmid
    #218778
    Purpose
    replicative E. coli-S. pyogenes shuttle plasmid for IPTG-inducible expression of the Firefly luciferase in a strain expressing lacI repressor
    Depositor
    Emmanuelle Charpentier
    Article
    Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1
    Insert
    ffluc
    Use
    Luciferase
    Expression
    Bacterial
    Mutation
    deletion of mrfp cassette, mutagenesis of the RBS…
    Promoter
    Plac(3)
    Available Since
    Sept. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    218778-plasmid-map-sequence-id-444099

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  11. pXGFPC-5 Pxyl-dcas9 (Spy)

    Plasmid
    #133316
    Purpose
    expresses dcas9 from Streptococcus pyogenes; repressed with 0.2% glucose, induced with 0.3% xylose; for homologous recombination at the xylose locus in Caulobacter crescentus; tetracycline resistance
    Depositor
    Michael Laub
    Article
    Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:
    Insert
    dcas9 (Streptococcus pyogenes)
    Use
    CRISPR
    Promoter
    xylose
    Available Since
    Feb. 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    133316-plasmid-map-sequence-id-266825

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  15. Neo-2/15-TGFa-SPMn-SpyTag003

    Plasmid
    #246650
    Purpose
    Expresses Neo-2/15-TGFa-SPMn-SpyTag003 in mammalian cells for calcium-induced NeissLock protein ligation to epidermal growth factor receptor (EGFR)
    Depositor
    Mark Howarth
    Article
    Lim et al ACS Chem Biol. 2025 Sep 15. doi: 10.1021
    Insert
    Neo-2/15-TGFa-SPMn-SpyTag003
    Tags
    Signal peptide and SpyTag003
    Expression
    Mammalian
    Mutation
    Construct contains the tPA signal peptide, Neo2/…
    Promoter
    CMV promoter
    Available Since
    Oct. 31, 2025
    Availability
    Academic Institutions and Nonprofits only
    246650-plasmid-map-sequence-id-491288

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  16. tPA-TGFa-SPMn-SpyTag003

    Plasmid
    #246649
    Purpose
    Expresses tPA-TGFa-SPMn-SpyTag003 in mammalian cells for calcium-induced NeissLock protein ligation to epidermal growth factor receptor (EGFR)
    Depositor
    Mark Howarth
    Article
    Lim et al ACS Chem Biol. 2025 Sep 15. doi: 10.1021
    Insert
    tPA-TGFa-SPMn-SpyTag003
    Tags
    Signal peptide and SpyTag003
    Expression
    Mammalian
    Mutation
    Construct contains the tPA signal peptide, Kringl…
    Promoter
    CMV promoter
    Available Since
    Oct. 31, 2025
    Availability
    Academic Institutions and Nonprofits only
    246649-plasmid-map-sequence-id-491286

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Showing: 201 - 220 of 578 results