We narrowed to 505 results for: SPY

1. pcDNA3.1-MF3958 Heavy chain-SpyTag003

   Plasmid

   #216303

   Purpose

   Expresses anti-HER2 MF3958 Heavy chain-SpyTag003 in mammalian cells. To be paired with MF3958 light chain to form the anti-HER2 MF3958 Fab

   Depositor

   Mark Howarth

   Article

   Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

   Insert

   MF3958 Heavy chain-SpyTag003
   

   Use

   Affinity Reagent/ Antibody

   Tags

   His6 tag, Signal peptide, and SpyTag003

   Expression

   Mammalian

   Mutation

   Promoter

   T7 promoter

   Available since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-Tras heavy chain-SpyTag003

   Plasmid

   #216293

   Purpose

   Expresses anti-HER2 Tras Heavy chain-SpyTag003 in mammalian cells. To be paired with Tras light chain to form the anti-HER2 Tras NoLink Fab

   Depositor

   Mark Howarth

   Article

   Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

   Insert

   Tras heavy chain-SpyTag003
   

   Use

   Affinity Reagent/ Antibody

   Tags

   His6 tag, Signal peptide, and SpyTag003

   Expression

   Mammalian

   Mutation

   Promoter

   T7 promoter

   Available since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3.1-39S Heavy chain-SpyTag003

   Plasmid

   #216296

   Purpose

   Expresses anti-HER2 39S Heavy chain-SpyTag003 in mammalian cells. To be paired with 39S light chain to form the anti-HER2 39S Fab

   Depositor

   Mark Howarth

   Article

   Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

   Insert

   39S Heavy chain-SpyTag003
   

   Use

   Affinity Reagent/ Antibody

   Tags

   His6 tag, Signal peptide, and SpyTag003

   Expression

   Mammalian

   Mutation

   Promoter

   T7 promoter

   Available since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. SpyTag-RBD Omicron XBB.1.5

   Plasmid

   #214724

   Purpose

   Expresses SARS-CoV-2 Omicron XBB.1.5 RBD (receptor-binding domain from Spike) with N-terminal SpyTag fusion in mammalian cells

   Depositor

   Mark Howarth

   Article

   Hills et al Nat Nanotechnol. 2024 May 6. doi: 10.103

   Insert

   Receptor-Binding Domain (S SARS-CoV-2)
   

   Use

   Tags

   SpyTag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. SpyTag003(M115G)-Talin rod-mCherry

   Plasmid

   #210026

   Purpose

   Expression of SpyTag003(M115G)-Talin rod(434-2541)-mCherry in mammalian cells

   Depositor

   Vesa Hytönen

   Article

   Rahikainen et al J Am Chem Soc. 2023 Nov 15;145(45):24459

   Insert

   SpyTag003(M115G)-Talin1 rod domain(434-2541)-mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   SpyTag003 M115G mutation to modify SpyCatcher003 …

   Promoter

   CMV

   Available since

   Dec. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. SpyTag003(M115A)-Talin rod-mCherry

   Plasmid

   #210025

   Purpose

   Expression of SpyTag003(M115A)-Talin rod(434-2541)-mCherry in mammalian cells

   Depositor

   Vesa Hytönen

   Article

   Rahikainen et al J Am Chem Soc. 2023 Nov 15;145(45):24459

   Insert

   SpyTag003(M115A)-Talin1 rod domain(434-2541)-mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   SpyTag003 M115A mutation to modify SpyCatcher003 …

   Promoter

   Available since

   Dec. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. SpyCatcher003(K31TAG)-Talin rod-mScarletI

   Plasmid

   #210022

   Purpose

   Expression of SpyCatcher003(K31TAG)-Talin rod(434-2541)-mScarletI in mammalian cells

   Depositor

   Vesa Hytönen

   Article

   Rahikainen et al J Am Chem Soc. 2023 Nov 15;145(45):24459

   Insert

   SpyCatcher003(K31TAG)-Talin rod(434-2541)-mScarletI
   

   Use

   Tags

   C-terminal mScarletI

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. EGFP-Talin head-SpyCatcher003(K31TAG)

   Plasmid

   #210023

   Purpose

   Expression of EGFP-Talin head(1-433)-SpyCatcher003(K31TAG) in mammalian cells

   Depositor

   Vesa Hytönen

   Article

   Rahikainen et al J Am Chem Soc. 2023 Nov 15;145(45):24459

   Insert

   EGFP-Talin head (1-433)-SpyCatcher003(K31TAG)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   SpyCatcher003 with K31TAG (Amber stop codon) muta…

   Promoter

   CMV

   Available since

   Dec. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pQE80L-His-SpyTag-XMod-Doc(WT)

   Plasmid

   #157675

   Purpose

   Expression of XMod-Doc wild type with His6x tag and SpyTag.

   Depositor

   Michael Nash

   Article

   Yang et al Nano Lett. 2020 Nov 16. doi: 10.1021/acs

   Insert

   XMod-Doc Wild type with Histag and SpyTag
   

   Use

   Tags

   Histag and SpyTag

   Expression

   Bacterial

   Mutation

   Promoter

   T5

   Available since

   Feb. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCbh-SpyCas9-2A-puro-pA_U6-sgRosa26

    Plasmid

    #149346

    Purpose

    Mammalian expression vector for SpyCas9, puromycin resistance and mouse Rosa26 guide RNA

    Depositor

    Ralf Kuehn

    Article

    Danner et al Open Biol. 2021 Jan;11(1):200283. doi: 1

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    Promoter

    Cbc

    Available since

    Jan. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCbh-SpyCas9-2A-puro-pA_U6-BbsI

    Plasmid

    #149347

    Purpose

    Mammalian expression vector for SpyCas9 and puromycin resistance

    Depositor

    Ralf Kuehn

    Article

    Danner et al Open Biol. 2021 Jan;11(1):200283. doi: 1

    Insert

    SpyCas9
    

    Use

    CRISPR

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh

    Available since

    Jan. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF)_IRES_eRF1(E55D)-HA

    Plasmid

    #182653

    Purpose

    Encodes codon-optimized AF mutant of M. mazei pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF),tRNACUAPyl & eRF1E55D used for amber codon suppression in mammalian cells

    Depositor

    Ivana Nikić-Spiegel

    Article

    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

    Inserts

    codon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
    PylT
    mutant eukaryotic release factor 1
    

    Use

    Tags

    HA tag and nuclear export signal (NES)

    Expression

    Mammalian

    Mutation

    E55D and Y306A/Y384F (AF) double mutant of Methan…

    Promoter

    CMV and U6

    Available since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. J135F_BattB_Vif(T27X4)-IRES-SpyTag-sfmCherry2(11)-PuroR

    Plasmid

    #200637

    Purpose

    Recombination plasmid for Matreyek Bxb1(GT) landing pad in dual landing pad cells

    Depositor

    Kenneth Matreyek

    Article

    Roelle et al ACS Synth Biol. 2023 Nov 3. doi: 10.1021

    Insert

    Vif
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    T27stop

    Promoter

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLKO.1-puro-U6-SpyCas9-TLR-MCV1-sgRNA1

    Plasmid

    #117405

    Purpose

    SpyCas9 sgRNA 1 targeting TLR2.0

    Depositor

    Erik Sontheimer

    Article

    Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

    Insert

    SpyCas9 sgRNA targeting TLR 2.0
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3.1-Tras heavy chain-GSG-SpyTag003

    Plasmid

    #216292

    Purpose

    Expresses anti-HER2 Tras Heavy chain-GSG-SpyTag003 in mammalian cells. To be paired with Tras light chain to form the anti-HER2 Tras Fab

    Depositor

    Mark Howarth

    Article

    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

    Insert

    Tras heavy chain-GSG-SpyTag003
    

    Use

    Affinity Reagent/ Antibody

    Tags

    His6 tag, Signal peptide, and SpyTag003

    Expression

    Mammalian

    Mutation

    Promoter

    T7 promoter

    Available since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. G1052C_BattB(GA)_EGFP_IRES-Spycatcher-sfmCherry3C(1-10)-P2A-shBleR

    Plasmid

    #200636

    Purpose

    Strong EGFP fluorescence recombination plasmid for Matreyek Bxb1(GA) landing pad in dual landing pad cells

    Depositor

    Kenneth Matreyek

    Article

    Roelle et al ACS Synth Biol. 2023 Nov 3. doi: 10.1021

    Insert

    EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV-RSV-GFP-U6-gRNA scaffold (SpyCas9)

    Plasmid

    #113039

    Purpose

    AAV vector; encodes GFP as well as a U6-driven gRNA scaffold (SpyCas9)

    Depositor

    Dominik Niopek

    Article

    Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

    Insert

    2x BbsI sites - SpCas9 scaffold, co-expressed GFP (transfection marker)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLKO.1-puro-U6-SpyCas9-TLR-MCV1-sgRNA2

    Plasmid

    #117406

    Purpose

    SpyCas9 sgRNA 2 targeting TLR2.0

    Depositor

    Erik Sontheimer

    Article

    Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

    Insert

    SpyCas9 sgRNA targeting TLR 2.0
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCAG-SpyTag-Flag-dNS-SRRM1-V5-mCherry

    Plasmid

    #235096

    Purpose

    SRRM1 lacking Nuclear Speckle domain (dNS-SRRM1) mCherry fusion protein without MCP domain

    Depositor

    Mitchell Guttman

    Article

    Bhat et al Nature. 2024 May;629(8014):1165-1173. do

    Insert

    pCAG-SpyTag-Flag-dNS-SRRM1-V5-mCherry (SRRM1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    The SRRM1 entry clone from DNASU was modified usi…

    Promoter

    CAG

    Available since

    April 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET23a-His-TEV-HaloTag-linker-SpyCatcher3

    Plasmid

    #235150

    Purpose

    Bacterial expression of His-tagged HaloTag-linker-SpyCatcher3 separated by a TEV protease recognition site.

    Depositor

    Giordano Lippi

    Article

    Zolboot et al Neuron. 2025 Apr 1:S0896-6273(25)00181-3

    Insert

    HaloTag linked to SpyCatcher3
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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