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Showing: 201 - 220 of 3266 results

  2. (194) pcDNA3.1-HA-nEPEA-(EAAAK)4-OGT(4,K852A)

    Plasmid
    #160742
    Purpose
    Expresses an inactive truncated OGT (4.5 TPRs, K852A) that can't bind O-GlcNAc fused to the EPEA nanobody. A different nanobody can be inserted by using Sgfl + Sgsl restriction enzymes.
    Depositor
    Christina Woo
    Article
    Ramirez et al ACS Chem Biol. 2020 Apr 17;15(4):1059-10
    Insert
    HA-nEPEA-(EAAAK)4-OGT(4,K852A)
    Use
    Tags
    HA-Tag
    Expression
    Mammalian
    Mutation
    Promoter
    T7/CMV
    Available since
    May 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    160742-plasmid-map-sequence-id-332924

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  6. 1358 pBabe puroL FKHR AAA delta DB

    Plasmid
    #10697
    Depositor
    William Sellers
    Insert
    FKHR AAA deltaDB (FOXO1 Human)
    Use
    Retroviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    T24A S256A S319A , deletion in aa 208-220
    Promoter
    Available since
    April 4, 2006
    Availability
    Academic Institutions and Nonprofits only

  7. Cenp-B DBD INCENP TSS/AAA mCherry

    Plasmid
    #45234
    Purpose
    Target INCENP (with TSS mutated to AAA) to centromeres via CENP-B DNA binding domain (DBD)
    Depositor
    Michael Lampson
    Article
    Wang et al J Cell Biol. 2011 Aug 22;194(4):539-49.
    Insert
    Cenp-B DBD, INCENP-ΔCEN with AAA mutation, mCherry
    Use
    Tags
    CENP-B DBD, DNA binding domain of CENP-B and mChe…
    Expression
    Mammalian
    Mutation
    INCENP C-terminal TSS mutated to AAA
    Promoter
    t7
    Available since
    Sept. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    45234-plasmid-map-sequence-id-84485

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  10. pcDNA3.1-N-Myc_DED/AAA 535-888 USP7

    Plasmid
    #131255
    Purpose
    Mammalian expression of USP7 UBL domains 1-3, with an N-terminal Myc tag and mutations in UBL2 (corresponding to DE758AA_D764A of the full-length protein)
    Depositor
    Roger Woodgate
    Article
    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d
    Insert
    Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    A mutant form of our pcDNA3.1-N-Myc_535-888 USP7 …
    Promoter
    Available since
    Dec. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    131255-plasmid-map-sequence-id-267709

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  14. (271) pcDNA3.1-HA-nEPEA-(EAAAK)3-OGA(544-706)

    Plasmid
    #168102
    Purpose
    HA- tagged EPEA nanobody fused to human OGA stalk domain residues (544-706). Used in combination with myc-OGA(1-400) to create split-OGA
    Depositor
    Christina Woo
    Article
    Ge et al Nat Chem Biol. 2021 Mar 8. pii: 10.1038/
    Insert
    HA-nEPEA-(EAAAK)3-OGA(544-706)
    Use
    Tags
    HA-tag
    Expression
    Mammalian
    Mutation
    OGA truncated to contain residues 544-706
    Promoter
    T7/CMV
    Available since
    May 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    168102-plasmid-map-sequence-id-333448

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  17. (367) pcDNA3.1-HA-nUbc-(EAAAK)4-OGA(544-706)

    Plasmid
    #168099
    Purpose
    HA- tagged Ubc6E nanobody fused to human OGA stalk domain residues (544-706). Used in combination with myc-OGA(1-400) to create split-OGA
    Depositor
    Christina Woo
    Article
    Ge et al Nat Chem Biol. 2021 Mar 8. pii: 10.1038/
    Insert
    HA-nUbc-OGA(544-706)
    Use
    Tags
    HA-tag
    Expression
    Mammalian
    Mutation
    OGA truncated to contain residues 544-706
    Promoter
    T7/CMV
    Available since
    June 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    168099-plasmid-map-sequence-id-336059

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Showing: 201 - 220 of 3266 results