We narrowed to 922 results for: cas12a

1. 3xNLS-NLP-cMyc-cMyc LbaCas12a

   Plasmid

   #182125

   Purpose

   pET21a protein expression vector for 3xNLS-NLP-cMyc-cMyc LbaCas12a in bacteria

   Depositor

   Scot Wolfe

   Article

   Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

   Insert

   3xNLS-NLP-cMyc-cMyc LbaCas12a
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pDGB3 alpha2 AtUBQ10:LbCas12a:Tnos (GB2479)

   Plasmid

   #160629

   Purpose

   TU for the expression of LbCas12a

   Depositor

   Diego Orzaez

   Insert

   AtUBQ10-:LbCpf1(Cas12a):Tnos
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   UBQ10

   Available since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCMV-T7-AsCas12a-P2A-EGFP (RTW2861)

   Plasmid

   #160140

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized AsCas12a with a c-terminal NLS(nucleoplasmin), 3x HA tag, and P2A-EGFP

   Depositor

   Benjamin Kleinstiver, Keith Joung

   Article

   Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

   Insert

   human codon optimized AsCas12a with NLS(nucleoplasmin)-3xHA-P2A-EGFP
   

   Use

   In vitro transcription; t7 promoter

   Tags

   NLS(nucleoplasmin)-3xHA-P2A-EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and T7

   Available since

   Feb. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pKEW336-MBP-TEV-MbCas12a-33362

   Plasmid

   #115670

   Purpose

   Expresses MbCas12 (from M. bovoculi 33362) in bacteria via T7 RNAP, for purification

   Depositor

   Jennifer Doudna

   Article

   Watters et al Science. 2018 Oct 12;362(6411):236-239.

   Insert

   MbCas12a
   

   Use

   CRISPR

   Tags

   MBP-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7 RNAP

   Available since

   Oct. 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. 3xNLS-NLP-cMyc-cMyc enAspCas12a

   Plasmid

   #182127

   Purpose

   pET21a protein expression vector for 3xNLS-NLP-cMyc-cMyc enAspCas12a in bacteria

   Depositor

   Scot Wolfe

   Article

   Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

   Insert

   3xNLS-NLP-cMyc-cMyc enAspCas12a
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pKEW321-MbCas12a-RFP-GFP-array

   Plasmid

   #115661

   Purpose

   Encodes an CRISPR array for MbCas12a targeting superfolder GFP and mRFP1

   Depositor

   Jennifer Doudna

   Article

   Watters et al Science. 2018 Oct 12;362(6411):236-239.

   Insert

   GFP and RFP array for MbCas12
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Plac

   Available since

   Oct. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. 3xNLS-NLP-cMyc-cMyc AspCas12a

   Plasmid

   #182122

   Purpose

   pET21a protein expression vector for 3xNLS-NLP-cMyc-cMyc AspCas12a in bacteria

   Depositor

   Scot Wolfe

   Article

   Luk et al GEN Biotechnol. 2022 Jun;1(3):271-284. d

   Insert

   3xNLS-NLP-cMyc-cMyc AspCas12a
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. SM283_EF1A-dEnAsCas12a-VPR-T2A-EGFP

   Plasmid

   #244218

   Purpose

   Mammalian expression of dEnAsCas12a-VPR with EGFP selectable marker

   Depositor

   Tim Reddy

   Insert

   dEnAsCas12a-VPR
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   dEnAsCas12a: E174R, S542R, K548R, and D908A

   Promoter

   Available since

   Sept. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. SM265_EF1A-dHyperLbCas12a-KRAB-T2A-EGFP

   Plasmid

   #244204

   Purpose

   Mammalian expression of dHyperLbCas12a-KRAB with EGFP selectable marker

   Depositor

   Tim Reddy

   Insert

   dHyperLbCas12a-KRAB
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   dHyperLbCas12a: D156R, D235R, E292R, E350R, and D…

   Promoter

   Available since

   Sept. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. SM272_EF1A-dHyperLbCas12a-VPR-T2A-EGFP

    Plasmid

    #244210

    Purpose

    Mammalian expression of dHyperLbCas12a-VPR with EGFP selectable marker

    Depositor

    Tim Reddy

    Insert

    dHyperLbCas12a-VPR
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    dHyperLbCas12a: D156R, D235R, E292R, E350R, and D…

    Promoter

    Available since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. SM282_EF1A-dEnAsCas12a-P300-T2A-BlastR

    Plasmid

    #244217

    Purpose

    Mammalian expression of dEnAsCas12a-P300 with Blasticidin resistance selectable marker

    Depositor

    Tim Reddy

    Insert

    dEnAsCas12a-P300
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    dEnAsCas12a: E174R, S542R, K548R, and D908A

    Promoter

    Available since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. SM274_EF1A-dEnAsCas12a-KRAB-T2A-EGFP

    Plasmid

    #244212

    Purpose

    Mammalian expression of dEnAsCas12a-KRAB with EGFP selectable marker

    Depositor

    Tim Reddy

    Insert

    dEnAsCas12a-KRAB
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    dEnAsCas12a: E174R, S542R, K548R, and D908A

    Promoter

    Available since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. SM268_EF1A-dHyperLbCas12a-P300-T2A-EGFP

    Plasmid

    #244206

    Purpose

    Mammalian expression of dHyperLbCas12a-P300 with EGFP selectable marker

    Depositor

    Tim Reddy

    Insert

    dHyperLbCas12a-P300
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    dHyperLbCas12a: D156R, D235R, E292R, E350R, and D…

    Promoter

    Available since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. SM269_EF1A-dHyperLbCas12a-P300-T2A-BlastR

    Plasmid

    #244207

    Purpose

    Mammalian expression of dHyperLbCas12a-P300 with Blasticidin resistance selectable marker

    Depositor

    Tim Reddy

    Insert

    dHyperLbCas12a-P300
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    dHyperLbCas12a: D156R, D235R, E292R, E350R, and D…

    Promoter

    Available since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. SM284_EF1A-dEnAsCas12a-VPR-T2A-BlastR

    Plasmid

    #244219

    Purpose

    Mammalian expression of dEnAsCas12a-VPR with Blasticidin resistance selectable marker

    Depositor

    Tim Reddy

    Insert

    dEnAsCas12a-VPR
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    dEnAsCas12a: E174R, S542R, K548R, and D908A

    Promoter

    Available since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. SM266_EF1A-dHyperLbCas12a-KRAB-T2A-BlastR

    Plasmid

    #244205

    Purpose

    Mammalian expression of dHyperLbCas12a-KRAB with Blasticidin resistance selectable marker

    Depositor

    Tim Reddy

    Insert

    dHyperLbCas12a-KRAB
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    dHyperLbCas12a: D156R, D235R, E292R, E350R, and D…

    Promoter

    Available since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. SM270_EF1A-dHyperLbCas12a-4xSID-T2A-EGFP

    Plasmid

    #244208

    Purpose

    Mammalian expression of dHyperLbCas12a-4xSID with EGFP selectable marker

    Depositor

    Tim Reddy

    Insert

    dHyperLbCas12a-4xSID
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    dHyperLbCas12a: D156R, D235R, E292R, E350R, and D…

    Promoter

    Available since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. SM275_EF1A-dEnAsCas12a-KRAB-T2A-BlastR

    Plasmid

    #244213

    Purpose

    Mammalian expression of dEnAsCas12a-KRAB with Blasticidin resistance selectable marker

    Depositor

    Tim Reddy

    Insert

    dEnAsCas12a-KRAB
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    dEnAsCas12a: E174R, S542R, K548R, and D908A

    Promoter

    Available since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. SM273_EF1A-dHyperLbCas12a-VPR-T2A-BlastR

    Plasmid

    #244211

    Purpose

    Mammalian expression of dHyperLbCas12a-VPR with Blasticidin resistance selectable marker

    Depositor

    Tim Reddy

    Insert

    dHyperLbCas12a-VPR
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    dHyperLbCas12a: D156R, D235R, E292R, E350R, and D…

    Promoter

    Available since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. SM281_EF1A-dEnAsCas12a-P300-T2A-EGFP

    Plasmid

    #244216

    Purpose

    Mammalian expression of dEnAsCas12a-P300 with EGFP selectable marker

    Depositor

    Tim Reddy

    Insert

    dEnAsCas12a-P300
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    dEnAsCas12a: E174R, S542R, K548R, and D908A

    Promoter

    Available since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge