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pG6(3'Pro) (P#1478)

Plasmid

#14660

Depositor

Matija Peterlin

Article

Taube et al Mol Cell Biol. 2002 Jan . 22(1):321-31.

Insert

UAS repeats

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 30, 2008

Availability

Academic Institutions and Nonprofits only
pFS_0414_pET30_MCS-BBaJ23100-LuxR-B0015term-pLux rR-Fluc-rrnBterm-pCat-tetR-Term-ptetA-Rluc-rrnBterm_FsRT-Cas1-Cas2_ARRAY2_FaqI

Plasmid

#117005

Purpose

Expresses FsRT-Cas1-Cas2 under pT7lac, Fluc under pLuxR and Rluc under ptetA promoter, encodes FsCRISPR array 2, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Fusicatenibacter saccharivorans RT-Cas1-Cas2

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

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Prab-3::NLS::GCaMP6s

Plasmid

#68119

Purpose

Nuclear Localized Fluorescent Calcium Indicator for pan-neuronal expression in C. Elegans

Depositor

Andrew Leifer

Article

Nguyen et al Proc Natl Acad Sci U S A. 2015 Dec 28. p

Insert

rab-3::NLS::GCaMP6s

Use

Tags

Expression

Worm

Mutation

Promoter

rab-3

Available since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pET21b-Caspase-3(C163S)

Plasmid

#90088

Purpose

Expresses caspase 3 with C163S to prevent autocatalysis in bacterial cells

Depositor

Clay Clark

Article

Pop et al Biochemistry. 2001 Nov 27;40(47):14224-3

Insert

Caspase 3 (CASP3 Human)

Use

Tags

His tag

Expression

Bacterial

Mutation

C163S

Promoter

Available since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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pJAK184.tetR.PT5-3.dCas9

Plasmid

#232482

Purpose

optimized ahTet inducible dCas9 for insertion into C. difficile genome

Depositor

James Collins

Article

Chua et al Microbiol Resour Announc. 2025 Apr 28:e0

Insert

Catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

TetR-PT5-3

Available since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR v2-sgL2HGDH-3

Plasmid

#230085

Purpose

Crispr knock out human L2HGDH gene

Depositor

Xingguo Zhu

Article

Xi et al Commun Biol. 2025 Jan 6;8(1):15. doi: 10

Insert

L2HGDH (L2HGDH Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pLKO-Tet-On shYAP1-3

Plasmid

#193666

Purpose

Tet inducible knockdown of YAP1

Depositor

William Hahn

Article

Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

Insert

YAP1 (YAP1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

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GFP-ataxin-3 C14A

Plasmid

#89977

Purpose

Expresses catalytic inactive GFP-tagged ataxin-3

Depositor

Nico Dantuma

Article

Pfeiffer et al EMBO J. 2017 Mar 8. pii: e201695151. doi

Insert

ataxin-3 (ATXN3 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

changed Cysteine 14 to Alanine

Promoter

Available since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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Lenti-sgTp53#3/Cre

Plasmid

#173622

Purpose

Expresses a Tp53-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Tp53 (Trp53 Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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V27 pLPS-3' HA - Acceptor

Plasmid

#11711

Depositor

Tony Pawson

Article

Colwill et al BMC Biotechnol. 2006 . 6():13.

Type

Empty backbone

Use

Cre/Lox; Acceptor vector

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

May 26, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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V1663 pLPS-3'RFP - Acceptor

Plasmid

#11701

Depositor

Tony Pawson

Article

Colwill et al BMC Biotechnol. 2006 . 6():13.

Type

Empty backbone

Use

Cre/Lox; Acceptor vector

Tags

RFP

Expression

Mammalian

Mutation

Promoter

Available since

May 26, 2006

Availability

Academic Institutions and Nonprofits only

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V518 pLPS-3'dMyc - Acceptor

Plasmid

#11714

Depositor

Tony Pawson

Article

Colwill et al BMC Biotechnol. 2006 . 6():13.

Type

Empty backbone

Use

Cre/Lox; Acceptor vector

Tags

2xMyc

Expression

Mammalian

Mutation

Promoter

Available since

May 26, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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V956 pLPS-3'ECFP - Acceptor

Plasmid

#11698

Depositor

Tony Pawson

Article

Colwill et al BMC Biotechnol. 2006 . 6():13.

Type

Empty backbone

Use

Cre/Lox; Acceptor vector

Tags

ECFP

Expression

Mammalian

Mutation

Promoter

Available since

May 26, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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V26 pLPS-3'Flag - Acceptor

Plasmid

#11704

Depositor

Tony Pawson

Article

Colwill et al BMC Biotechnol. 2006 . 6():13.

Type

Empty backbone

Use

Cre/Lox; Acceptor vector

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

May 26, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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pLKO.1 puro shPRDM14-3

Plasmid

#193696

Purpose

Constitutive lentiviral expression of PRDM14 shRNA

Depositor

William Hahn

Article

Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

Insert

PRDM14 (PRDM14 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-U6-shR-E1-3

Plasmid

#19803

Depositor

Zuoshang Xu

Article

Qiu et al BMC Biotechnol. 2008 . 8():77.

Insert

shRNA

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 21, 2008

Availability

Academic Institutions and Nonprofits only
pEGFP-U6-shR-E2-3

Plasmid

#19811

Depositor

Zuoshang Xu

Article

Qiu et al BMC Biotechnol. 2008 . 8():77.

Insert

shRNA

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 21, 2008

Availability

Academic Institutions and Nonprofits only
SRa-HA-SUMO2 (Sentrin 3)

Plasmid

#17360

Purpose

Mammalian expression of human SUMO2 with HA tag

Depositor

Edward Yeh

Article

Kamitani et al J Biol Chem. 1998 Feb 6. 273(6):3117-20.

Insert

SUMO2 (SUMO2 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

April 4, 2008

Availability

Academic Institutions and Nonprofits only

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PLKO.1-STAU1-3'UTR

Plasmid

#136049

Purpose

STAU1 shRNA (Targeting 3'UTR) inserted into the PLKO.1 plasmid (CCATCACCACTGCTTTCTCTT)

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

STAU1 (STAU1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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YIp211-P30-His-POL30-3'

Plasmid

#99546

Purpose

Yeast integrative vector for expression of His-tagged POL30 (PCNA); URA3 marker

Depositor

Helle Ulrich

Article

Davies et al Methods Mol Biol. 2012;920:543-67. doi:

Insert

POL30 (POL30 Budding Yeast)

Use

Tags

6xHis

Expression

Yeast

Mutation

Promoter

POL30

Available since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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