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Plasmid#160992PurposeExpress fulllength of mouse p150 proteinDepositorInsertCHAF1A (Chaf1a Mouse)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUCM-AAVS1-TO-hNGN2
Plasmid#105840PurposeDonor construct for introduction of NGN2 into AAVS1 safe harbor site and iPSC differentiation to cortical neuronDepositorInsertsUseCRISPR and TALENTagsExpressionMutationPromoterCAG, EF-1alpha, and TRE3GAvailable sinceApril 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSyn.iGluSnFr.WPRE.SV40
Plasmid#98929PurposeAAV expression of glutamate sensor from human synapsin promoter. ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorHas ServiceAAV1, AAV5, and AAV9InsertiGluSnFr
UseAAVTagsExpressionMammalianMutationPromoterhSynAvailable sinceJan. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
EID1-pGex-2TK
Plasmid#22689DepositorInsertE1A-like inhibitor of differentiation 1 (EID1 Human)
UseTagsGSTExpressionBacterialMutationwt cDNAPromoterAvailable sinceDec. 3, 2009AvailabilityAcademic Institutions and Nonprofits only -
pENN.AAV.GFAP.iGluSnFr.WPRE.SV40
Plasmid#98930PurposeAAV expression of glutamate sensor from GFAP promoter. ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorHas ServiceAAV1, AAV5, and AAV9InsertiGluSnFr
UseAAVTagsExpressionMammalianMutationPromoterGFAPAvailable sinceJan. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.Flex.iGluSnFr.WPRE.SV40
Plasmid#98932PurposeCre dependent AAV expression of glutamate sensor from GFAP promoter ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorHas ServiceAAV1, AAV5, and AAV9InsertiGluSnFr
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceJan. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSyn.Flex.iGluSnFr.WPRE.SV40
Plasmid#98931PurposeCre dependent AAV expression of glutamate sensor from human synapsin promoter. ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorHas ServiceAAV1, AAV5, and AAV9InsertiGluSnFr
UseAAVTagsExpressionMammalianMutationPromoterhSynAvailable sinceJan. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pWPXL-p150-MIRΔ
Plasmid#160995PurposeExpress mouse p150 protein with MIR deletion mutationDepositorInsertCHAF1A (Chaf1a Mouse)
UseTagsGFPExpressionMammalianMutationMIR (HP1-interacting domain) deletionPromoterAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pWPXL-p150-PIP2Δ
Plasmid#160994PurposeExpress mouse p150 protein with PIP2 deletion mutationDepositorInsertCHAF1A (Chaf1a Mouse)
UseTagsGFPExpressionMammalianMutationPIP2 motif deletionPromoterAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pWPXL-p150-PIP1Δ
Plasmid#160993PurposeExpress mouse p150 protein with PIP1 deletion mutationDepositorInsertCHAF1A (Chaf1a Mouse)
UseTagsGFPExpressionMammalianMutationPIP1 (PCNA-interacting protein) motif deletionPromoterAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRSET.GltI253-cpGFP.L1LV/L2NP
Plasmid#41733Purposea single-wavelength intracellular glutamate sensor constructed from E. coli Gltl and cpGFP. For bacterial cytoplasmic expressionDepositorInsertGltI based Glutamate Biosensor
UseGlutamate biosensorTags6xHis, T7 epitope, and Xpress tagExpressionBacterialMutationcpGFP146 variant from EcMBP165-cpGFP.PPYF.pRSET i…PromoterT7Available sinceJan. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
CYLBL_Neo_CAGdCas9-KRAB
Plasmid#220439PurposeCAG-dCas9-KRAB knock-in using TALEN at CLYBL locusDepositorInsertdCas9
UseCRISPR and TALENTagsKRABExpressionMammalianMutationD10A and H840APromoterAvailable sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV(MinDis).iGluSnFR
Plasmid#41732PurposeSingle-wavelength extracellular glutamate sensor constructed from E. coli Gltl and cpGFP. Membrane-displayed. ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorInsertGltI based Glutamate Biosensor
UseGlutamate biosensorTagsMyc, PDGFR transmembrane helix (aa 513-561), and …ExpressionMammalianMutationcpGFP146 variant from EcMBP165-cpGFP.PPYF.pRSET i…PromoterCMVAvailable sinceJan. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pUCM-CLYBL-hNIL
Plasmid#105841PurposeDonor construct for introduction of hNIL factors to CLYBL safe harbor site and iPSC differentiation to motor neuronDepositorInsertsUseCRISPR and TALENTagsExpressionMutationPromoterCAG, EF-1alpha, and TRE3GAvailable sinceJuly 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
CLYBL-(Ef1a-SBP-LNGFR-T2A-mApple)-(CAG-rtTA)-(TRE-hNIL))
Plasmid#105842PurposeDonor construct for introduction of hNIL factors and SBP-LNGFR for streptavidin bead enrichmentDepositorInsertsUseCRISPR and TALENTagsExpressionMutationPromoterCAG, EF-1alpha, and TRE3GAvailable sinceApril 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFastbac-Flag-BRCA1
Plasmid#223228PurposeInsect expression construct for Flag-tagged BRCA1 protein production and purificationDepositorInsertBreast Cancer 1 (BRCA1 Human)
UseTagsFlagExpressionInsectMutationPromoterPolyhedrinAvailable sinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTL
Plasmid#203176PurposeCentromeric yeast expression vector, leucine selectionDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceAug. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pBS cPitx2 (CT#73)
Plasmid#13927DepositorInsertpitx2 (PITX2 Chicken)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 9, 2007AvailabilityAcademic Institutions and Nonprofits only -
RCAS (A) Flu cPitx2 (CT#704)
Plasmid#13928DepositorInsertpitx2 (PITX2 Chicken)
UseRetroviral; Avian expressionTagsFlu (3xHA)ExpressionMutationPromoterAvailable sinceFeb. 15, 2007AvailabilityAcademic Institutions and Nonprofits only