We narrowed to 470 results for: plasmid cloning by pcr

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A1-20B1(P004)

Plasmid

#184056

Purpose

This plasmid carries Yn situ preamplifier A1-20B1 sequence and is used for nickase based synthesis.

Depositor

C. Ron Yu

Article

Wu et al Cell Rep Methods. 2022 Apr 18;2(4):10020

Insert

A1-20B1 preamplifier

Use

Synthetic Biology

Available Since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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pBx1-VHH template1-3XMyc-Spacer

Plasmid

#176208

Purpose

PCR template for generating CeVICA VHH/nanobody library

Depositor

Aviv Regev

Article

Chen et al Nat Commun. 2021 Sep 17;12(1):5506. doi:

Insert

VHH template1-3XMyc-Spacer

Tags

Myc tag

Expression

Bacterial

Promoter

T7

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pBx1-VHH template2-3XMyc-Spacer

Plasmid

#176209

Purpose

PCR template for generating CeVICA VHH/nanobody library

Depositor

Aviv Regev

Article

Chen et al Nat Commun. 2021 Sep 17;12(1):5506. doi:

Insert

VHH template2-3XMyc-Spacer

Tags

Myc tag

Expression

Bacterial

Promoter

T7

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pEntry Tshld(FKBP F36V)-GFP

Plasmid

#53260

Purpose

pEntry Tshld(FKBP F36V)-GFP is a product of BP recombination reaction between attB flanked Tshld(F36V) system-encoded PCR product and pDonor 201 plasmid.

Depositor

Matthew Pratt

Article

Lin et al Chembiochem. 2014 Mar 11. doi: 10.1002/c

Insert

Tshld(FKBP F36V)-GFP

Tags

EGFP, Flag, and HA

Mutation

FKBP F36V

Available Since

May 29, 2014

Availability

Academic Institutions and Nonprofits only

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pCCM937

Plasmid

#58982

Purpose

Expresses avr-15 sgRNA in C.elegans.

Depositor

Craig Mello

Article

Kim et al Genetics. 2014 May 30. pii: genetics.114

Insert

avr-15 sgRNA

Use

CRISPR

Expression

Worm

Promoter

U6

Available Since

July 30, 2014

Availability

Academic Institutions and Nonprofits only
pCCM936

Plasmid

#58981

Purpose

Expresses avr-14 sgRNA in C.elegans.

Depositor

Craig Mello

Article

Kim et al Genetics. 2014 May 30. pii: genetics.114

Insert

avr-14 sgRNA

Use

CRISPR

Expression

Worm

Promoter

U6

Available Since

July 30, 2014

Availability

Academic Institutions and Nonprofits only
L0-T7 Empty target Tp901

Plasmid

#195885

Purpose

Level 0 Empty Tp901 target: attP_Tp901-BpiI-attB_Tp901 with golden gate linker in pBLUNT plasmid.

Depositor

Jennifer Nemhauser

Article

Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

Insert

Empty Tp901 Target

Use

Synthetic Biology

Available Since

Sept. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pDSHA-ARF4-Myc

Plasmid

#67350

Purpose

Mammalian expression of hArf4 with C-term Myc tag

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARF4 (ARF4 Human)

Tags

Myc

Expression

Mammalian

Mutation

V53A, L123P and M134I mutations were unintended P…

Promoter

CMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDEST47-ARF4-GFP

Plasmid

#67392

Purpose

Mammalian expression of hArf4 with C-term GFP tag

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARF4 (ARF4 Human)

Tags

GFP

Expression

Mammalian

Mutation

V53A, L123P and M134I mutations were unintended P…

Promoter

CMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pES134

Plasmid

#127551

Purpose

Variant of pKD13. Lambda Red recombineering PCR template plasmid carrying an F3-aadA-F3 cassette orthogonal to FRT-KanR-FRT in pKD13

Depositor

Emmanuele Severi, Gavin Thomas

Article

Wu et al Microbiology (Reading). 2024 Mar;170(3).

Insert

F3-flanked spectinomycin resistance cassette

Use

Synthetic Biology

Expression

Bacterial

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pXf20pemIK-GW

Plasmid

#126576

Purpose

Vector for gene expression in Xylella fastidiosa, includes pemI/pemK toxin-antitoxin system for plasmid stability, Gateway cloning compatible

Depositor

Lindsey Burbank, Drake Stenger

Article

Burbank et al Phytopathology. 2016 Aug;106(8):928-36.

Type

Empty backbone

Expression

Bacterial

Available Since

June 7, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-anti-GFP(LaG16) PAGER(Gi)

Plasmid

#230008

Purpose

Expresses anti-GFP PAGER(Gi) in mammalian cells

Depositor

Alice Ting

Article

Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-

Insert

MT1-LaG16-TEVcs-ALFA-hM4D

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pJet-gR-X4ex2-DTS

Plasmid

#177154

Purpose

Donor plasmid for knock-in of MT-C34 peptide into exon-2 of CXCR4

Depositor

Dmitriy Mazurov

Article

Maslennikova et al mBio. 2022 Jan 25:e0358921. doi: 10.1128

Insert

MT-C34-pA with CXCR4 arms of homology

Use

Pcr cloning

Promoter

none

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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TOPOII p48 (DM#261)

Plasmid

#15026

Depositor

Douglas Melton

Insert

p48 (Ptf1a Mouse)

Expression

Bacterial

Available Since

Oct. 18, 2007

Availability

Academic Institutions and Nonprofits only
GGE177

Plasmid

#120766

Purpose

Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Terminator of gene expression for position 1 (T1_Xpr2)

Depositor

Tristan Rossignol

Article

Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

Insert

T1_Xpr2

Use

Cloning/assembly plasmid

Available Since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

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GGE085

Plasmid

#120738

Purpose

Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Promoter for gene expression in position1 (P1_Tef)

Depositor

Tristan Rossignol

Article

Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

Insert

P1_Tef

Use

Cloning/assembly plasmid

Available Since

Oct. 10, 2019

Availability

Academic Institutions and Nonprofits only

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GGE002

Plasmid

#120739

Purpose

Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Promoter for gene expression in position1 (P1_GAPdh)

Depositor

Tristan Rossignol

Article

Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

Insert

P1_GAPdh

Use

Cloning/assembly plasmid

Available Since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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GGE080

Plasmid

#120774

Purpose

Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Terminator of gene expression for position 3 (T3_Lip2)

Depositor

Tristan Rossignol

Article

Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

Insert

T3_Lip2

Use

Cloning/assembly plasmid

Available Since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

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GGE001

Plasmid

#120740

Purpose

Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Promoter for gene expression in position1 (P1_PGM)

Depositor

Tristan Rossignol

Article

Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

Insert

P1_PGM

Use

Cloning/assembly plasmid

Available Since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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GGE014

Plasmid

#120765

Purpose

Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Terminator of gene expression for position 1 (T1_Lip2)

Depositor

Tristan Rossignol

Article

Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

Insert

T1_Lip2

Use

Cloning/assembly plasmid

Available Since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

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