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We narrowed to 264 results for: plasmid transfection

Showing: 201 - 220 of 264 results

  1. CAG-Cremyc-2A-GFP

    Plasmid
    #116879
    Purpose
    Express Cre enzyme in mammalian cells with floxed genes to achieve somatic knockout. GFP is simultaneously expressed for convenient tracking the transfected cells.
    Depositor
    Qiang Lu
    Article
    Geng et al Nat Commun. 2018 Jul 13;9(1):2707. doi:
    Insert
    Cremyc-2A-GFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    May 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    116879-plasmid-map-sequence-id-243398

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  2. pIRES_IBB-mCherry-IRES-HA-mAID-nanobody

    Plasmid
    #117720
    Purpose
    Expression of nuclear transfection marker IBB-mCherry and HA-tagged mAID-nanobody from one mRNA
    Depositor
    Joerg Mansfeld
    Article
    Daniel et al Nat Commun. 2018 Aug 17;9(1):3297. doi:
    Inserts
    mAID-nanobody
    IBB-mCherry
    Use
    Tags
    importin-beta bining domain (IBB) and triple HA
    Expression
    Mammalian
    Mutation
    codon optimized mAID for murine expression
    Promoter
    CMV and None, IRES-driven
    Available Since
    Dec. 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    117720-plasmid-map-sequence-id-227032

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  3. tdTomato/pTREX-b

    Plasmid
    #68709
    Purpose
    Expresses tdTomato in pTREX-b vector which confers resistance to blasticidin. This vector is used for cloning a specific sgRNA by BamHI, to transfect Cas9-expressing Trypanosoma cruzi epimastigotes.
    Depositor
    Roberto Docampo
    Article
    Lander et al MBio. 2015 Jul 21;6(4). pii: e01012-15.
    Insert
    tdTomato
    Use
    Expression of tdtomato red fluorescence protein i…
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    Oct. 8, 2015
    Availability
    Academic Institutions and Nonprofits only
    68709-plasmid-map-sequence-id-125636

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  7. IL1RN gRNA3

    Plasmid
    #113132
    Purpose
    Expresses gRNA targeting the IL1RN promoter (SpyCas9 scaffold)
    Depositor
    Dominik Niopek
    Article
    Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s
    Insert
    IL1RN guideRNA 3 (SpCas9 scaffold), co-expressed GFP (transfection marker)
    Use
    AAV, CRISPR, and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Nov. 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    113132-plasmid-map-sequence-id-224944

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  8. IL1RN gRNA4

    Plasmid
    #113133
    Purpose
    Expresses gRNA targeting the IL1RN promoter (SpyCas9 scaffold)
    Depositor
    Dominik Niopek
    Article
    Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s
    Insert
    IL1RN guideRNA 4 (SpCas9 scaffold), co-expressed GFP (transfection marker)
    Use
    AAV, Synthetic Biology, and TALEN
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Nov. 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    113133-plasmid-map-sequence-id-226956

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  9. IL1RN gRNA2

    Plasmid
    #113131
    Purpose
    Expresses gRNA targeting the IL1RN promoter (SpyCas9 scaffold)
    Depositor
    Dominik Niopek
    Article
    Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s
    Insert
    IL1RN guideRNA 1 (SpCas9 scaffold), co-expressed GFP (transfection marker)
    Use
    AAV, CRISPR, and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Nov. 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    113131-plasmid-map-sequence-id-224942

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  10. pBP Omomer

    Plasmid
    #113169
    Purpose
    Retroviral vector for expressing Omomer (tamoxifen inducible Omomyc), by DNA transfection or retroviral infection.
    Depositor
    Sergio Nasi
    Article
    Soucek et al Cancer Res. 2002 Jun 15;62(12):3507-10.
    Insert
    Omomer: a fusion contruct of Omomyc and MER (a mutant estrogen receptor, reponsive to tamoxifen) (MYC Human)
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    E57T, E64I, R70Q, R71N
    Promoter
    Available Since
    Aug. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    113169-plasmid-map-sequence-id-217942

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  12. CDK1as_T2A_Zeo

    Plasmid
    #118596
    Purpose
    One of three plasmids required to create CDK1as human cells by single transfection (One shot system). CDK1as cDNA and Zeocin resistance cassette flanked by Sleeping Beauty transposon.
    Depositor
    William Earnshaw
    Article
    Saldivar et al Science. 2018 Aug 24;361(6404):806-810.
    Insert
    CDK1
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Jan. 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    118596-plasmid-map-sequence-id-229650

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  16. pPB-Ins-U6p-sgRNAentry-EF1Ap-TetOn3G-IRES-Neo

    Plasmid
    #183411
    Purpose
    piggyBAC-based sgRNA entry (Esp3I) and Tet-On 3G transactivator expression vector. Insert protospacer and transfect with CRISPRa (183409) or CRISPRi (183410) plasmid for inducible epigenome editing.
    Depositor
    Azim Surani
    Article
    Tang et al Nat Cell Biol. 2022 Apr;24(4):448-460. d
    Insert
    Tet-on 3G
    Use
    CRISPR and Synthetic Biology; Piggybac
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1A
    Available Since
    May 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    183411-plasmid-map-sequence-id-361257

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  17. IL1RN gRNA1

    Plasmid
    #113130
    Purpose
    Expresses gRNA targeting the IL1RN promoter (SpyCas9 scaffold)
    Depositor
    Dominik Niopek
    Article
    Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s
    Insert
    IL1RN guideRNA 1 (SpCas9 scaffold), co-expressed GFP (transfection marker)
    Use
    AAV, CRISPR, and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 for gRNA expression, RSV for GFP expression
    Available Since
    March 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    113130-plasmid-map-sequence-id-217998

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  18. Synapsin sHRPb-NLG in FSW lentiviral vector

    Plasmid
    #73148
    Purpose
    Small fragment of split HRP fused to the extracellular terminus of NLG1. This is a lentiviral plasmid, but can also be used for transfections. Syanpsin promoter.
    Depositor
    Alice Ting
    Article
    Martell et al Nat Biotechnol. 2016 May 30. doi: 10.103
    Insert
    sHRPb-NLG (Nlgn1 Mouse, Synthetic)
    Use
    Lentiviral
    Tags
    V5 tag
    Expression
    Mammalian
    Mutation
    Promoter
    Synapsin
    Available Since
    June 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    73148-plasmid-map-sequence-id-134528

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  20. HA-IFITM3-F8-TAG

    Plasmid
    #122475
    Purpose
    expresses human IFITM3 with unnatural amino acid incorporated site specifically in response to the amber codon TAG in mammalian cells when co-transfected with tRNA synthetase/tRNA pair.
    Depositor
    Howard Hang
    Article
    Spence et al Nat Chem Biol. 2019 Jan 14. pii: 10.1038
    Insert
    IFITM3 (IFITM3 Human)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Changed Phenylalanine 8 to amber codon
    Promoter
    Available Since
    March 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    122475-plasmid-map-sequence-id-237716

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Showing: 201 - 220 of 264 results