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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 13,974 results for: FLU;

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Showing: 2201 - 2220 of 13974 results

  1. Cx30-msfGFP

    Plasmid
    #69019
    Purpose
    Expresses human Cx30 (GJB6 CDS) with a 7 amino acid linker on the C-terminus of the Connexin linking to monomerized superfolder Green Fluorescent Protein. Expression driven by CMV promoter.
    Depositor
    David Spray
    Article
    Stout RF et al J Biol Chem. 2015 Aug 11. pii: jbc.M115.
    Insert
    Cx30 (GJB6 Human)
    Tags
    V206K monomerized super folder GFP
    Expression
    Mammalian
    Promoter
    c
    Available Since
    Oct. 23, 2015
    Availability
    Academic Institutions and Nonprofits only
    69019-plasmid-map-sequence-id-127981

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  5. pDual-eGFP(Y203)

    Plasmid
    #63559
    Purpose
    Expression of the Mostaza (yellow) spectral variant of eGFP in bacteria and in mammalian cells. Used as a reporter for gene targeting and recombineering in mammalian cells.
    Depositor
    Richard S Myers
    Article
    Valledor et al IUBMB Life. 2012 Aug;64(8):684-9. doi: 1
    Insert
    His-T7-eGFP(Y203)
    Use
    Lentiviral
    Tags
    His6 and T7
    Expression
    Bacterial and Mammalian
    Mutation
    Changed threonine at position 203 with respect to…
    Promoter
    CMV-EF1α hybrid (CEF)
    Available Since
    April 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  10. pBetaActin-FKBP-mScarlet-KIFC1motor

    Plasmid
    #191334
    Purpose
    Expresses the fluorescently labeled minus-end directed KIFC1 motor domain fused to the dimerization domain FKBP to recruit it to the plasma membrane by using the chemical dimerization system FKBP-FRB
    Depositor
    Frank Bradke
    Article
    Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:
    Insert
    FKBP-mScarlet-KIFC1 (KIFC1 Human)
    Expression
    Mammalian
    Mutation
    motor domain aa 125-673; N593K
    Available Since
    Jan. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    191334-plasmid-map-sequence-id-381755

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  14. pLS2965

    Plasmid
    #122534
    Purpose
    Expression of a C. elegan codon optimized fluorescent protein (CemOrange2) fused to a Golgi localized gene (aman-2) under the intestinal specific promoter nhx-2
    Depositor
    Gary Silverman
    Article
    Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do
    Insert
    Pnhx-2_aman-2::CemOrange2 (aman-2 Synthetic, Nematode)
    Expression
    Bacterial
    Promoter
    Pnhx-2
    Available Since
    March 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    122534-plasmid-map-sequence-id-238120

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  17. pcDNA3-FLAG-LplA(AAG)

    Plasmid
    #61307
    Purpose
    Contains FLAG epitope for immunofluorescence detection of resorufin ligase for mammalian cell expression
    Depositor
    Alice Ting
    Article
    Liu et al Proc Natl Acad Sci U S A. 2014 Oct 28;11
    Insert
    E. coli lipoic acid ligase
    Tags
    6xHis, Flag, T7 tag, and Xpress epitope
    Expression
    Mammalian
    Mutation
    E20A, F147A, H149G
    Promoter
    CMV
    Available Since
    March 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    61307-plasmid-map-sequence-id-99628

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  19. pBAD mApple

    Plasmid
    #91772
    Purpose
    Bacterial expression of a fluorescent protein, monomeric Apple (mApple). Note: Addgene identified several mutations compared to the final published sequence.
    Depositor
    Erik Rodriguez, Roger Tsien
    Article
    Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:
    Insert
    monomeric Apple
    Tags
    Hexa-Histidine tag, Xpress epitope for detection …
    Expression
    Bacterial
    Mutation
    R97K, E152S, A180S, V207T compared to mApple (FP …
    Promoter
    araBAD
    Available Since
    June 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    91772-plasmid-map-sequence-id-181643

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  20. pRSET a sfMatryoshCaMP6s-T78H

    Plasmid
    #100021
    Purpose
    Bacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference LSSmOrange nested within the reporting superfolder cpGFP-T78H
    Depositor
    Wolf Frommer
    Article
    Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10
    Insert
    sfMatryoshCaMP6s
    Tags
    6x HIS tag and Xpress_EK tag
    Expression
    Bacterial
    Mutation
    cpEGFP replaced with superfolder cpGFP and T78H m…
    Promoter
    T7
    Available Since
    Oct. 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    100021-plasmid-map-sequence-id-194294

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Showing: 2201 - 2220 of 13974 results