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We narrowed to 5,937 results for: plasmid dna

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Showing: 5501 - 5550 of 5937 results

  13. pNEU-hMbPylRS-4xU6M15

    Plasmid
    #105830
    Purpose
    Plasmid encoding for the Pyrrolysine translational pair that enables efficent incoporation of Pyl-like click amino acids in response to the amber codon into proteins in mammalian cells
    Depositor
    Irene Coin
    Article
    Serfling et al Nucleic Acids Res. 2017 Nov 21. pii: 464
    Inserts
    Humanized MbPylRS
    tRNAM15
    Expression
    Mammalian
    Mutation
    (Y271A, Y349F)MbPylRS
    Promoter
    CMV and U6
    Available Since
    March 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    105830-plasmid-map-sequence-id-466939

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  16. cycLuc_TEVS

    Plasmid
    #119207
    Purpose
    Circularly permutated firefly luciferase with TEV cleavage site (Luciferase reporter for detection of proteolytic activity)
    Depositor
    Roman Jerala
    Article
    Fink et al Nat Chem Biol. 2019 Feb;15(2):115-122. d
    Insert
    cyclic luciferase with TEVs cleavage site
    Use
    Luciferase
    Tags
    6xHis and AU1 tag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    April 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    119207-plasmid-map-sequence-id-232048

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  19. anti-FX(a) Emi HC

    Plasmid
    #113665
    Purpose
    For transient expression of Emicizumab sequence (CAS# 1610943-06-0, KEGG# D10821), IgG4 bispecific antibody targeting FIX(a) & FX(a). Must be co-expressed with the anti-FIX(a) Emi HC and the Emi LC.
    Depositor
    Robert Peters
    Article
    Leksa et al J Thromb Haemost. 2019 Mar 18. doi: 10.1
    Insert
    Emicizumab anti-FX/FXa heavy chain
    Expression
    Mammalian
    Promoter
    CMV Promoter
    Available Since
    March 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    113665-plasmid-map-sequence-id-225229

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  20. anti-FIX(a) Emi HC

    Plasmid
    #113664
    Purpose
    For transient expression of Emicizumab sequence (CAS# 1610943-06-0, KEGG# D10821), IgG4 bispecific antibody targeting FIX(a) & FX(a). Must be co-expressed with the anti-FX(a) Emi HC and the Emi LC.
    Depositor
    Robert Peters
    Article
    Leksa et al J Thromb Haemost. 2019 Mar 18. doi: 10.1
    Insert
    Emicizumab anti-FIX/FIXa heavy chain
    Expression
    Mammalian
    Promoter
    CMV Promoter
    Available Since
    March 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    113664-plasmid-map-sequence-id-225231

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  21. dCas9-NLS-gs10-cTRb413

    Plasmid
    #188261
    Purpose
    Plasmid ensures constitutive expression of the C-terminal fragment of split TRbeta (aa 413-460), fused to the catalytically inactive Cas9 (dCas9) protein, a flexible GS linker and the SV40 NLS signal at the N-terminus
    Depositor
    Roman Jerala
    Article
    Rihtar et al Nat Chem Biol. 2022 Sep 26. doi: 10.1038
    Insert
    cTRb413 (TXNRD2 Human)
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Oct. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    188261-plasmid-map-sequence-id-374572

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  28. pJ204-loxP-EF1a-tagBFP-HygR-lox2272-BGHpA-siteT9-HDR

    Plasmid
    #154827
    Purpose
    Landing pad for Cre/lox-based recombinase-mediated cassette exchange (RMCE) in CHO cells, donor plasmid for CRISPR/Cas9-mediated targeted integration in CHO cells (site T9)
    Depositor
    Lise Marie Grav
    Article
    Sergeeva et al ACS Synth Biol. 2020 Aug 24. doi: 10.102
    Inserts
    TagBFP
    HygR
    ZsGreen1-DR
    Use
    CRISPR and Cre/Lox
    Tags
    PEST
    Expression
    Mammalian
    Promoter
    CMV, EF1a, and SV40 early promoter
    Available Since
    Aug. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    154827-plasmid-map-sequence-id-306202

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  29. pAAV-CMV-HBA-RIBEYE-eGFP

    Plasmid
    #241988
    Purpose
    Human RIBEYE cDNA cloned inframe into a pAAV-GFP vector driven by hybrid CMV-enhancer-human-beta-actin promoter (CMV-HBA) and containing downstream WPRE, Ampicillin Resistance
    Depositor
    Tobias Moser
    Article
    Kapoor et al eLife13:RP98254
    Insert
    RIBEYE-GFP (CTBP2 Human)
    Use
    AAV
    Tags
    eGFP
    Expression
    Mammalian
    Promoter
    hybrid CMV-enhancer-human-beta-actin promoter (CM…
    Available Since
    Oct. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    241988-plasmid-map-sequence-id-484630

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  30. pJ204-loxP-EF1a-mCherry-NeoR-lox2272-BGHpA-siteA-HDR

    Plasmid
    #154826
    Purpose
    Landing pad for Cre/lox-based recombinase-mediated cassette exchange (RMCE) in CHO cells, donor plasmid for CRISPR/Cas9-mediated targeted integration in CHO cells (site A)
    Depositor
    Lise Marie Grav
    Article
    Sergeeva et al ACS Synth Biol. 2020 Aug 24. doi: 10.102
    Inserts
    mCherry
    NeoR
    ZsGreen1-DR
    Use
    CRISPR and Cre/Lox
    Tags
    PEST
    Expression
    Mammalian
    Promoter
    CMV, EF1a, and SV40 early promoter
    Available Since
    Aug. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    154826-plasmid-map-sequence-id-306204

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  33. CaMKIV-dCT

    Plasmid
    #126422
    Purpose
    Plasmid expresses constitutively active form of CaMK IV (C-terminal regulatory domains removed making the enzyme constitutively active)
    Depositor
    Douglas Black
    Article
    Xie et al Nature. 2001 Apr 19;410(6831):936-9. doi
    Insert
    Calcium/calmodulin-dependent protein kinase type IV (CAMK4 Human)
    Tags
    FLAG
    Expression
    Mammalian
    Available Since
    July 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    126422-plasmid-map-sequence-id-249664

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Showing: 5501 - 5550 of 5937 results