We narrowed to 3,367 results for: aaas

1. pPBO.ACT004

   Plasmid

   #182714

   Purpose

   Constituitvely expressed CasRx crRNA cloning vector, extended 3' terminator region

   Depositor

   Joseph Schoeniger

   Article

   Otoupal et al Nucleic Acids Res. 2022 Aug 11. pii: 666

   Insert

   CasRx crRNA cloning backbone
   

   Use

   CRISPR

   Expression

   Bacterial

   Mutation

   Catalytically deactivated R295A, H300A, R849A, H8…

   Promoter

   pJ23119 (TTGACAGCTAGCTCAGTCCTAGGTATAATACTAGT)

   Available Since

   Aug. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. 1056K

   Plasmid

   #183137

   Purpose

   Plasmid supports expression of 2 gRNAs targeting D.suzukii sxl, and 1 gRNA targeting D.suzukii bTub,Opie-mVenus tagged, and can be integrated with pBac.

   Depositor

   Omar Akbari

   Article

   Kandul et al GEN Biotechnology (2022) 1:4, 372-385

   Insert

   U6.3-gRNAs[sxl, bTub]
   

   Use

   CRISPR

   Expression

   Insect

   Available Since

   June 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSIN-Arkadia-gRNA3

   Plasmid

   #180364

   Purpose

   targeting mouse Arkadia gene

   Depositor

   Dan Littman

   Article

   Xu et al J Exp Med. 2021 Nov 1;218(11). pii: 2126

   Insert

   Arkadia targeting gRNA (Rnf111 Mouse)
   

   Use

   Retroviral

   Expression

   Mammalian

   Promoter

   human U6

   Available Since

   June 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBBK22 pCAS-Tyr-[gRNA: 5=ARS308] (SplitHygR, AmpR)

   Plasmid

   #179006

   Purpose

   Sp.Cas9 and gRNA yeast expression vector with ARS308 gRNA pre-cloned. Selection =SplitHygromycin resistance

   Depositor

   Vincent Martin

   Article

   Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

   Insert

   S. pyogenes Cas9
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Yeast

   Available Since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBBK15 pCAS-Tyr-[gRNA: 5=ARS308] (SplitKanR, AmpR)

   Plasmid

   #178999

   Purpose

   Sp.Cas9 and gRNA yeast expression vector with ARS308 gRNA pre-cloned. Selection =SplitKanamycin resistance

   Depositor

   Vincent Martin

   Article

   Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

   Insert

   S. pyogenes Cas9
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Yeast

   Available Since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX459-sg-CMV162

   Plasmid

   #179917

   Purpose

   All in one Cas9 plasmid with puromycin resistance and a single guide RNA targeting cytomegaloviral promoter site 162.

   Depositor

   Johan Sosa, Indeever Madireddy

   Article

   Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

   Insert

   CMV promoter sgRNA
   

   Use

   CRISPR

   Tags

   2A-Puro and 3XFLAG

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   April 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFB_ROSA26_SpCas9_gRNAs

   Plasmid

   #174703

   Purpose

   Plasmid containing separate SpCas9 gRNAs for targeted excision of inserted STOP Cassette upstream of reporter alleles found in Ai14 and Ai6 mice. ITRs flank the cassette for easy vector creation.

   Depositor

   Beverly L. Davidson

   Article

   Lang et al Nat Commun. 2019 Jul 30;10(1):3415. doi:

   Insert

   SpCas9 dual gRNAs
   

   Use

   AAV

   Available Since

   Nov. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSIN-Tpi1

   Plasmid

   #175940

   Purpose

   knockout mouse Tpi1

   Depositor

   Dan Littman

   Article

   Wu et al Cell. 2020 Aug 6;182(3):641-654.e20. doi

   Insert

   Tpi1 gRNA (Tpi1 Mouse)
   

   Use

   Retroviral

   Available Since

   Nov. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSIN-Olfr2

   Plasmid

   #175935

   Purpose

   knockout mouse Olfr2

   Depositor

   Dan Littman

   Article

   Wu et al Cell. 2020 Aug 6;182(3):641-654.e20. doi

   Insert

   Olfr2 gRNA (Or6a2 Mouse)
   

   Use

   Retroviral

   Available Since

   Nov. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-CAG-GFP-mirNega

    Plasmid

    #163705

    Purpose

    pAAV plasmid to induce expression of universal negative control micro-RNA and EGFP

    Depositor

    Michisuke Yuzaki

    Article

    Ito-Ishida et al J Neurosci. 2020 Nov 4;40(45):8746-8766.

    Insert

    mirNega and EGFP
    

    Use

    AAV

    Promoter

    CAG

    Available Since

    March 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. p3E-U6a-U6c-mfn2 guide#2

    Plasmid

    #121994

    Purpose

    An entry vector with U6a and U6c promoter driving mfn2 guide RNAs expression

    Depositor

    Qing Deng

    Article

    Zhou et al J Cell Sci. 2020 Sep 4;133(17). pii: jcs

    Insert

    mfn2 gRNA
    

    Use

    CRISPR

    Available Since

    Sept. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTBL581 GA

    Plasmid

    #126951

    Purpose

    To target a protospacer with GA at the cut site.

    Depositor

    Chang Liu

    Article

    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

    Insert

    spacer against human genome with GA at cut site
    

    Expression

    Mammalian

    Promoter

    human U6

    Available Since

    May 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. gh249

    Plasmid

    #106856

    Purpose

    expression of gRNA targeting PIGV

    Depositor

    Eric-Paul Bennett

    Article

    Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

    Insert

    PIGV (PIGV Human)
    

    Expression

    Mammalian

    Available Since

    May 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAPM-D4 miR30-SUV39h2 ts2

    Plasmid

    #115884

    Purpose

    SUV39h2 knockdown

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

    Insert

    SUV39h2 shRNA (SUV39H2 Human)
    

    Use

    Lentiviral

    Promoter

    SFFV

    Available Since

    Jan. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. gRNA[Tra].1026G

    Plasmid

    #112689

    Purpose

    express gRNA targeting Tra under dU6-3 promoter

    Depositor

    Omar Akbari

    Article

    Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

    Insert

    U6.3-gRNA[Tra] (tra Fly)
    

    Use

    CRISPR

    Available Since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. gh147

    Plasmid

    #106814

    Purpose

    expression of gRNA targeting KDELC1

    Depositor

    Eric-Paul Bennett

    Article

    Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

    Insert

    KDELC1 (POGLUT2 Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. gh50

    Plasmid

    #106723

    Purpose

    expression of gRNA targeting ST3GAL2

    Depositor

    Eric-Paul Bennett

    Article

    Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

    Insert

    ST3GAL2 (ST3GAL2 Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCR1324

    Plasmid

    #111120

    Purpose

    Expresses WDR48_+_39093886.23-P1P2 sgRNA in pCR1068

    Depositor

    Jacob Corn

    Article

    Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

    Insert

    WDR48 gRNA (WDR48 Human)
    

    Use

    Lentiviral

    Available Since

    Oct. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. TRIN-E-shTAF12.364

    Plasmid

    #105572

    Purpose

    Dox-inducible mir30 MYB shRNA/dsRED expression with GFP marker and neo resistance

    Depositor

    Christopher Vakoc

    Article

    Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

    Insert

    TAF12 shRNA
    

    Use

    RNAi and Retroviral

    Expression

    Mammalian

    Promoter

    TRE

    Available Since

    May 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pKDsgRNA-fsaA

    Plasmid

    #102286

    Purpose

    Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting fsaA.

    Depositor

    Michael Lynch

    Article

    Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

    Insert

    fsaA gRNA
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    pTet

    Available Since

    April 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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