We narrowed to 3,505 results for: cgas

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BUB1B A8.1 gRNA

Plasmid

#90555

Purpose

3rd generation lentiviral gRNA plasmid targeting human BUB1B

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

BUB1B (Guide Designation A8.1)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 17, 2017

Availability

Academic Institutions and Nonprofits only

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CCNB1 B3.1 gRNA

Plasmid

#90576

Purpose

3rd generation lentiviral gRNA plasmid targeting human CCNB1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

CCNB1 (Guide Designation B3.1)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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DSN1 C11.1 gRNA

Plasmid

#90656

Purpose

3rd generation lentiviral gRNA plasmid targeting human DSN1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

DSN1 (Guide Designation C11.1)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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CENPF C6.1 gRNA

Plasmid

#90617

Purpose

3rd generation lentiviral gRNA plasmid targeting human CENPF

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

CENPF (Guide Designation C6.1)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 13, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_HMG20A_2

Plasmid

#86319

Purpose

Encodes gRNA for 3' target of human HMG20A

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against HMG20A (HMG20A Human)

Use

CRISPR

Available Since

Jan. 31, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_HMG20A_1

Plasmid

#86318

Purpose

Encodes gRNA for 3' target of human HMG20A

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against HMG20A (HMG20A Human)

Use

CRISPR

Available Since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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shCTL-mlpx-neo

Plasmid

#65233

Purpose

encodes a non-targeting control shRNA

Depositor

Gerardo Ferbeyre

Article

Deschenes-Simard et al Genes Dev. 2013 Apr 15;27(8):900-15. doi

Insert

non-coding shRNA

Expression

Mammalian

Promoter

PGK

Available Since

Aug. 12, 2015

Availability

Academic Institutions and Nonprofits only

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AP54-5

Plasmid

#65599

Purpose

co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans fbf-2 gene

Depositor

Geraldine Seydoux

Article

Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

Insert

sgRNA for APs12

Use

CRISPR

Expression

Worm

Promoter

U6

Available Since

June 19, 2015

Availability

Academic Institutions and Nonprofits only
AP54-3

Plasmid

#65597

Purpose

co-expression of Cas9 and a sgRNA targeting 3'end of C.elegans fbf-2 gene

Depositor

Geraldine Seydoux

Article

Paix et al Genetics. 2014 Dec;198(4):1347-56. doi:

Insert

sgRNA for APs12

Use

CRISPR

Expression

Worm

Promoter

U6

Available Since

June 9, 2015

Availability

Academic Institutions and Nonprofits only
CMV-sgE1TSS-short-3'box

Plasmid

#92165

Purpose

sgRNA expression vector for Evx1as RNA tethering assay

Depositor

Xiaohua Shen

Article

Luo et al Cell Stem Cell. 2016 May 5;18(5):637-52.

Insert

Evx1as short isoform

Use

CRISPR

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-sgNCKAP1-1

Plasmid

#210133

Purpose

knock out NCKAP1 in mammalian cells

Depositor

Boyi Gan

Article

Liu et al Nat Cell Biol. 2023 Mar;25(3):404-414. d

Insert

Nck-associated protein 1 (NCKAP1 Human)

Use

CRISPR

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pORANGE Tubb3-GFP KI

Plasmid

#131497

Purpose

Endogenous tagging of β3 Tubulin: C-terminal (amino acid position: STOP codon)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Tubb3, Tubb3 Mouse, Rat)

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pX459-HypaCas9-ANLN_sgRNA

Plasmid

#183874

Purpose

pX459V2.0-HypaCas9 plasmid with ANLN sgRNA for N-terminal tagging of anillin in human cells.

Depositor

Alisa Piekny

Article

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

ANLN sgRNA spacer (ANLN Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEX-SaCas9-U6-sgGrin1

Plasmid

#124852

Purpose

Mutagenesis of Grin1

Depositor

Larry Zweifel

Article

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

Grin1 (Grin1 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available Since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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BLADE-182

Plasmid

#134914

Purpose

sgRNA targeting GFP to be used in nanoblade system

Depositor

Philippe Mangeot, Théophile Ohlmann, Emiliano Ricci

Article

Mangeot et al Nat Commun. 2019 Jan 3;10(1):45. doi: 10

Insert

GFP

Use

CRISPR and Synthetic Biology

Promoter

U6

Available Since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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Cas9-GFP_sg_mTfeb

Plasmid

#79006

Purpose

Expresses WT Cas9 and GFP, along with a sgRNA to mouse Tfeb.

Depositor

Reuben Shaw

Article

Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

Insert

sgTfeb (Tfeb Mouse)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

June 22, 2016

Availability

Academic Institutions and Nonprofits only

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px459 sgAtg5

Plasmid

#175023

Purpose

CRISPR-Cas9 plasmid targeting exon 2 of human Atg5.

Depositor

David Tumbarello

Article

Assar et al Front Cell Dev Biol. 2020 Aug 4;8:733. d

Insert

ATG5 (ATG5 Human)

Expression

Mammalian

Available Since

Oct. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pX330-PITCh-GOLGA2

Plasmid

#207791

Purpose

Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the N-terminus of GOLGA2 for knock-in.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

sgRNA Targeting N-terminus of GOLGA2 (GOLGA2 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Dec. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pENN496_miniCRISPRcharm_dSaCas9

Plasmid

#220837

Purpose

mini CRISPRcharm expression in mammalian cells

Depositor

Jonathan Weissman

Article

Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

Insert

mini CRISPRcharm

Use

CRISPR

Tags

P2A-TagBFP

Expression

Mammalian

Mutation

N/A

Promoter

EFS

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pLVUTHshGATA1-tTR-KRAB

Plasmid

#11650

Purpose

Tet-regulated (Tet-on) lentiviral vector for shGATA1 (hUbiquitin promoter) - 3rd generation

Depositor

Patrick Aebischer, Didier Trono

Article

Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

Insert

hUbiquitin C, GFP, tTR-KRAB, shRNA against GATA1, Tet-on (GATA1 Human)

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Available Since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only

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