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Showing: 4441 - 4480 of 8321 results

  1. PVALB mAb_BCN14.1.1A2_LC_Rat Ig Kappa

    Plasmid
    #182895
    Purpose
    Mammalian expression of PVALB rat mAb LC gene
    Depositor
    Heng Zhu
    Insert
    anti-PVALB (human) light chain (PVALB Rat)
    Use
    Affinity Reagent/ Antibody
    Tags
    Expression
    Mammalian
    Mutation
    No
    Promoter
    CMV
    Available since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    182895-plasmid-map-sequence-id-360643

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  2. pLenti-PGK-NXPH4 (neo)

    Plasmid
    #192078
    Purpose
    Lentivirus for expression of NXPH4
    Depositor
    Roland Friedel
    Insert
    NXPH4 (NXPH4 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    hPGK
    Available since
    Dec. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    192078-plasmid-map-sequence-id-379235

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  4. pET-15b(+) Toxoplasma gondii (delta prenylation motif)

    Plasmid
    #170789
    Purpose
    Expresses pET-15b(+) Toxoplasma gondii (delta prenylation motif)
    Depositor
    Kalle Gehring
    Insert
    Toxoplasma gondii PRL
    Use
    Tags
    N-terminal His-tag
    Expression
    Bacterial
    Mutation
    Deleted nucleotides coding for 4 C-terminal amino…
    Promoter
    AmpR, lacI
    Available since
    Sept. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    170789-plasmid-map-sequence-id-337771

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  5. pB6-Rosa26-cre_dre_reporter_1_2

    Plasmid
    #185966
    Purpose
    B6J Rosa26-cre dre reporter 1.2 allele targeting vector, low copy number
    Depositor
    Mario Capecchi
    Insert
    sfGFP, tdTomato
    Use
    Cre/Lox and Mouse Targeting; Dre/rox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    185966-plasmid-map-sequence-id-367346

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  6. pB6-Rosa26-cre_dre_reporter_1_1b

    Plasmid
    #185965
    Purpose
    B6J Rosa26-cre dre reporter 1.1b allele targeting vector, low copy number
    Depositor
    Mario Capecchi
    Insert
    sfGFP, tdTomato
    Use
    Cre/Lox and Mouse Targeting; Dre/rox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    185965-plasmid-map-sequence-id-367345

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  7. pB6-Rosa26-cre_dre_reporter_1_1a

    Plasmid
    #185964
    Purpose
    B6J Rosa26-cre dre reporter 1.1a allele targeting vector, low copy number
    Depositor
    Mario Capecchi
    Insert
    sfGFP, tdTomato
    Use
    Cre/Lox and Mouse Targeting; Dre/rox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    185964-plasmid-map-sequence-id-367344

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  8. pB6-Rosa26-cre_dre_reporter_1_1

    Plasmid
    #185963
    Purpose
    B6J Rosa26-cre dre reporter 1.1 allele targeting vector, low copy number
    Depositor
    Mario Capecchi
    Insert
    EGFP, tdTomato
    Use
    Cre/Lox and Mouse Targeting; Dre/rox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    185963-plasmid-map-sequence-id-367343

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  9. FNF-CAG-L-3xSV40pA-L-tdTomato shuttle vector

    Plasmid
    #182346
    Purpose
    PacI-flanked CAG-LSL expression cassette with a FNF for positive selection
    Depositor
    Mario Capecchi
    Insert
    tdTomato
    Use
    Mouse Targeting; Flp / frt
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    182346-plasmid-map-sequence-id-358221

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  10. R26-CAG-rox-FNF-bpA-3pA-rox-tdTomato

    Plasmid
    #180154
    Purpose
    tdTomato dre reporter with Rosa26 recombineering homology arms
    Depositor
    Mario Capecchi
    Insert
    tdTomato
    Use
    Mouse Targeting; Dre/rox
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    Feb. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    180154-plasmid-map-sequence-id-355319

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  11. SDCBPA-c021

    Plasmid
    #175485
    Purpose
    Protein expression in bacterial cells. PDZ1 and PDZ2 domains (truncated at C-terminus), G111-F275.
    Depositor
    Opher Gileadi
    Insert
    SDCBP (SDCBP Human)
    Use
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175485-plasmid-map-sequence-id-353207

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  12. SDCBPA-c011

    Plasmid
    #175483
    Purpose
    Protein expression in bacterial cells. PDZ1 and PDZ2 domains (truncated at C-terminus), A106-F275.
    Depositor
    Opher Gileadi
    Insert
    SDCBP (SDCBP Human)
    Use
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    175483-plasmid-map-sequence-id-353170

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  13. pAAV-CMV-FLEX-SaCas9-U6-sgVamp1

    Plasmid
    #159919
    Purpose
    Mutagenesis of Vamp1
    Depositor
    Larry Zweifel
    Insert
    Vamp1 (Vamp1 Mouse)
    Use
    AAV, CRISPR, and Mouse Targeting
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    159919-plasmid-map-sequence-id-317336

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  14. pAAV-CMV-FLEX-SaCas9-U6-sgNtn1

    Plasmid
    #159907
    Purpose
    Mutagenesis of Netrin1
    Depositor
    Larry Zweifel
    Insert
    Netrin1 (Ntn1 Mouse)
    Use
    AAV, CRISPR, and Mouse Targeting
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    159907-plasmid-map-sequence-id-317313

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  15. pLL1272

    Plasmid
    #129287
    Purpose
    Expression plasmid with pLL1270 as backbone and alpha-Xylp_1211 and alpha-L-Araf_1120 as inserts
    Depositor
    Lee Lynd
    Inserts
    alpha-Xylp_1211 (Ga0256695_ 1211)
    alpha-L-Araf_1120
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Clostridium thermocellum DSM1313 Cellobiose Phosp…
    Available since
    March 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    129287-plasmid-map-sequence-id-260451

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  16. pENTR-Hu_TTK1(WT)

    Plasmid
    #136354
    Purpose
    Human TTK (Mps1) cDNA in Gateway pENTR
    Depositor
    Guillermo de Cárcer
    Insert
    TTK (TTK Human)
    Use
    Gateway entry vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    136354-plasmid-map-sequence-id-270746

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  17. pLV-EF1a-V5-Ccdc167

    Plasmid
    #120242
    Purpose
    Expresses V5-tagged Ccdc167 in lentiviral vector
    Depositor
    Larry Gerace
    Insert
    Ccdc167 (Ccdc167 Mouse)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    120242-plasmid-map-sequence-id-232814

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  18. IRES-EGFP-Flag-hRabin8

    Plasmid
    #118073
    Purpose
    Expresses Flag-tagged human Rabin8 in mammalian cells
    Depositor
    Ken-Ichi Takemaru
    Insert
    human Rabin8 (RAB3IP Human)
    Use
    Lentiviral
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Promoter
    EF1 alpha
    Available since
    Dec. 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    118073-plasmid-map-sequence-id-230364

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  19. pCSD_SpCas9MT3-FKBP_SL

    Plasmid
    #107302
    Purpose
    Expresses R1335K mutant SpCas9 fused to FKBP in mammalian cells
    Depositor
    Scot Wolfe
    Insert
    SpCas9
    Use
    CRISPR
    Tags
    FKBP
    Expression
    Mammalian
    Mutation
    R1335K and fused to FKBP
    Promoter
    CMV IE94
    Available since
    Nov. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    107302-plasmid-map-sequence-id-204632

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  20. pCSD_FRB-TALETS3-3xFLAG-2xNLS

    Plasmid
    #107306
    Purpose
    Expresses TALE-VEGFA-TS3 fused to FRB in mammalian cells
    Depositor
    Scot Wolfe
    Insert
    TALE_VEGFA-TS3
    Use
    CRISPR
    Tags
    3x Flag, 2xNLS and FRB
    Expression
    Mammalian
    Mutation
    Promoter
    CMV IE94
    Available since
    Nov. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    107306-plasmid-map-sequence-id-204787

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  21. pHH0103 CEP164 WW domain

    Plasmid
    #104276
    Purpose
    Bacterial expression of WW domain from CEP164
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    CEP164 WW domain (CEP164 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available since
    Aug. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    104276-plasmid-map-sequence-id-203766

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  22. pHH0103 CEP164 WW domain

    Plasmid
    #104300
    Purpose
    Bacterial expression of WW domain from CEP164
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    CEP164 WW domain (CEP164 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available since
    Aug. 28, 2018
    Availability
    Academic Institutions and Nonprofits only
    104300-plasmid-map-sequence-id-203483

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  23. pBSIIKS_HIS3_I

    Plasmid
    #109062
    Purpose
    yeast shuttle vector with deletable CEN6-ARSH4 cassette in pBIIKS backbone. LoxP site upstream of HIS3. For teaching purposes.
    Depositor
    Colin Logie
    Insert
    HIS3 (HIS3 Budding Yeast, Zygosaccharomyces rouxii)
    Use
    Cre/Lox
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    HIS3
    Available since
    Aug. 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    109062-plasmid-map-sequence-id-209541

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  24. hC73M-HA

    Plasmid
    #111528
    Purpose
    Expresses human KCNB1 C73M in mammalian cells
    Depositor
    Federico Sesti
    Insert
    human C73M-Kv2.1 + HA (KCNB1 Human)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    C73M
    Promoter
    CMV
    Available since
    June 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    111528-plasmid-map-sequence-id-213350

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  25. hC73L-HA

    Plasmid
    #111527
    Purpose
    Expresses human KCNB1 C73L in mammalian cells
    Depositor
    Federico Sesti
    Insert
    human C73L-Kv2.1-HA (KCNB1 Human)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    C73L
    Promoter
    CMV
    Available since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    111527-plasmid-map-sequence-id-211650

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  26. pHH0103 HECW2 WW domain #1

    Plasmid
    #104244
    Purpose
    Bacterial expression of WW domain #1 from HECW2
    Depositor
    Sachdev Sidhu, Marius Sudol
    Insert
    HECW2 WW domain #1 (HECW2 Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available since
    Feb. 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    104244-plasmid-map-sequence-id-204378

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  27. His8:MBP-tev-Trp-eK-Flv

    Plasmid
    #83390
    Purpose
    bacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studies
    Depositor
    Nico Dissmeyer
    Insert
    flavin binding protein MioC
    Use
    Synthetic Biology
    Tags
    3xHA, 8xHis, and MBP
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    83390-plasmid-map-sequence-id-175070

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  28. His8:MBP-tev-Asp-eK-Flv

    Plasmid
    #83389
    Purpose
    bacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studies
    Depositor
    Nico Dissmeyer
    Insert
    flavin binding protein MioC
    Use
    Synthetic Biology
    Tags
    3xHA, 8xHis, and MBP
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    83389-plasmid-map-sequence-id-175068

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  29. His8:MBP-tev-His-eK-Flv

    Plasmid
    #83388
    Purpose
    bacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studies
    Depositor
    Nico Dissmeyer
    Insert
    flavin binding protein MioC
    Use
    Synthetic Biology
    Tags
    3xHA, 8xHis, and MBP
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    83388-plasmid-map-sequence-id-175065

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  30. His8:MBP-tev-Lys-eK-Flv

    Plasmid
    #83387
    Purpose
    bacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studies
    Depositor
    Nico Dissmeyer
    Insert
    flavin binding protein MioC
    Use
    Synthetic Biology
    Tags
    3xHA, 8xHis, and MBP
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    83387-plasmid-map-sequence-id-175064

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  31. His8:MBP-tev-Leu-eK-Flv

    Plasmid
    #83386
    Purpose
    bacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studies
    Depositor
    Nico Dissmeyer
    Insert
    flavin binding protein MioC
    Use
    Synthetic Biology
    Tags
    3xHA, 8xHis, and MBP
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    83386-plasmid-map-sequence-id-175060

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  32. His8:MBP-tev-Arg-eK-Flv

    Plasmid
    #83385
    Purpose
    bacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studies
    Depositor
    Nico Dissmeyer
    Insert
    flavin binding protein MioC
    Use
    Synthetic Biology
    Tags
    3xHA, 8xHis, and MBP
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    83385-plasmid-map-sequence-id-173975

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  33. His8:MBP-tev-Phe-eK-Flv

    Plasmid
    #83384
    Purpose
    bacterial expression vector pVP16 containing a derivative of E. coli lacZ fused with E. coli flavin binding protein MioC to be used in N-end rule in vitro ubiquitination studies
    Depositor
    Nico Dissmeyer
    Insert
    flavin binding protein MioC
    Use
    Synthetic Biology
    Tags
    3xHA, 8xHis, and MBP
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    83384-plasmid-map-sequence-id-173972

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  34. pCASCADE-gltA1-gltA2-GapAP1

    Plasmid
    #87150
    Purpose
    gltA1-gltA2-GapAP1 targeting guide RNA E. coli , Low Phosphate Induction
    Depositor
    Michael Lynch
    Insert
    gltA1-gltA2-gapAP1 guide array
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    E . coli ugpB gene promoter, low phosphate induct…
    Available since
    March 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    87150-plasmid-map-sequence-id-174939

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  35. pJM102: pHps2 RFP on pCM66T

    Plasmid
    #74741
    Purpose
    pCM66T with promoter pHps2 driving RFP expression (maxRFP = BBa_E1010)
    Depositor
    Mary Lidstrom
    Insert
    RFP (BioBrick part number BBa_E1010)
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pHps2 (extracted from Methylovorus glucosetrophus…
    Available since
    May 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    74741-plasmid-map-sequence-id-141458

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  36. pJK103

    Plasmid
    #73265
    Purpose
    Produces Acetobacter aceti 1023 hypothetical acetic acid resistance protein B, C-terminal His6 tag (AaAarBH6)
    Depositor
    T. J. Kappock
    Insert
    hypothetical acetic acid resistance protein B
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    April 22, 2016
    Availability
    Academic Institutions and Nonprofits only
    73265-plasmid-map-sequence-id-134809

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  37. pJK610

    Plasmid
    #72531
    Purpose
    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase glutaminase subunit (AaPurQ); large subunit wutg C-terminal His6 tag (AaPurLH6)
    Depositor
    T. J. Kappock
    Inserts
    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    formylglycinamidine ribonucleotide synthetase, large subunit
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    T7 and n/a
    Available since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    72531-plasmid-map-sequence-id-132619

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  38. pJK540

    Plasmid
    #72446
    Purpose
    Produces Acetobacter aceti 1023 small hypothetical protein with N-terminal His6 tag (H6AaOrfX); N5-carboxyaminoimidazole ribonucleotide mutase (AaPurE1); NCAIR synthetase (AaPurK)
    Depositor
    T. J. Kappock
    Inserts
    hypothetical protein
    N5-carboxyaminoimidazole ribonucleotide mutase
    N5-carboxyaminoimidazole ribonucleotide synthetase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7 and n/a
    Available since
    March 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    72446-plasmid-map-sequence-id-133865

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  39. pJK552

    Plasmid
    #72451
    Purpose
    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase with inserted Val2 (AaPurE1-V2ins); N5-carboxyaminoimidazole ribonucleotide synthetase, Val319>Phe mutant (AaPurK-V319F)
    Depositor
    T. J. Kappock
    Inserts
    N5-carboxyaminoimidazole ribonucleotide mutase
    N5-carboxyaminoimidazole ribonucleotide synthetase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Val319 to Phe and inserted Val2
    Promoter
    T7
    Available since
    March 3, 2016
    Availability
    Academic Institutions and Nonprofits only
    72451-plasmid-map-sequence-id-133883

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  40. pJK141

    Plasmid
    #73316
    Purpose
    Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with Pas2p signal sequence, c-myc tag, and His6-tag
    Depositor
    T. J. Kappock
    Insert
    delta-12 fatty acid acetylenase
    Use
    Tags
    His6, Pas2p signal sequence, and c-myc
    Expression
    Yeast
    Mutation
    synthetic gene
    Promoter
    AOX1
    Available since
    Feb. 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    73316-plasmid-map-sequence-id-135024

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Showing: 4441 - 4480 of 8321 results