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Showing: 2221 - 2240 of 66152 results

  7. T4 Lysozyme T21C K124C WT*

    Plasmid
    #18243
    Depositor
    Brian Matthews
    Insert
    T4 Lysozyme (e Bacteriophage T4)
    Expression
    Bacterial
    Mutation
    T21C K124C WT*
    Available since
    Aug. 26, 2008
    Availability
    Academic Institutions and Nonprofits only

  10. pCMV-PE6a

    Plasmid
    #207851
    Purpose
    Mammalian expression of PE6a prime editor
    Depositor
    David Liu
    Publication
    Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PE6a
    Tags
    SV40 bpNLS and SV40 bpNLS, c-Myc NLS
    Expression
    Mammalian
    Mutation
    Ec48rt(E60K, K87E, E165D, D243N, R267I, E279K, K3…
    Available since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    207851-plasmid-map-sequence-id-450977

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  11. hLRP1-pEZT-BM

    Plasmid
    #177985
    Purpose
    Expression the full-length ectodomain of human LRP1 [residue 1-4414] in mammalian cells
    Depositor
    Lora Hooper
    Publication
    Bang et al Science. 2021 Sep 17;373(6561):eabf9232.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    LDL receptor related protein 1 (LRP1 Human)
    Tags
    No
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Dec. 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    177985-plasmid-map-sequence-id-350002

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  12. 5xUAS:GAP-mCherry-P2A-NfsB_Vv F70A/F108Y,he:tagBFP2

    Plasmid
    #158653
    Purpose
    making transgenics expressing a UAS driven mCherry and NTR 2.0
    Depositor
    Jeff Mumm
    Publication
    Sharrock et al Nat Methods. 2022 Feb;19(2):205-215. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    5xUAS:GAP-mCherry-P2A-NfsB_Vv F70A/F108Y;he:BFP
    Use
    Tol2-based transgenic expression in zebrafish/oth…
    Mutation
    F70A/F108Y
    Available since
    Sept. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    158653-plasmid-map-sequence-id-311196

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  13. pLifeAct-HyPer7

    Plasmid
    #136464
    Purpose
    Mammalian expression of F-actin targeted ultrasensitive hydrogen peroxide indicator HyPer7 fused with LifeAct peptide for optical imaging
    Depositor
    Vsevolod Belousov
    Publication
    Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HyPer7
    Tags
    LifeAct
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    136464-plasmid-map-sequence-id-271538

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  14. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR

    Plasmid
    #118154
    Purpose
    Catalytically inactive Cas9 from S. pyogenes with P2A-BlastR under the EF1a core promoter, and cloning backbone for sgRNA. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Jorge Ferrer
    Insert
    KRAB-dCas9-P2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    See depositor comments below
    Promoter
    EF1a core and U6
    Available since
    March 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    118154-plasmid-map-sequence-id-239465

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  15. pDisplay-GACh2.0

    Plasmid
    #106073
    Purpose
    express the genetically-encoded fluorescent ACh indicator GACh2.0 in mammalian cells
    Depositor
    Yulong Li
    Publication
    Jing et al Nat Biotechnol. 2018 Sep;36(8):726-737.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GPCR activation based ACh sensor (GACh) 2.0
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    March 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    106073-plasmid-map-sequence-id-260902

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  16. pCI-neo Fluc EGFP

    Plasmid
    #90170
    Purpose
    EGFP-tagged constructs to monitor the aggregation state of the sensors and the ability of cells to solubilize or degrade the aggregated proteins
    Depositor
    Franz-Ulrich Hartl
    Publication
    Gupta et al Nat Methods. 2011 Sep 4;8(10):879-84. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    FLuc EGFP
    Expression
    Mammalian
    Available since
    Aug. 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    90170-plasmid-map-sequence-id-226521

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  17. pCI-neo FlucDM EGFP

    Plasmid
    #90172
    Purpose
    EGFP-tagged constructs to monitor the aggregation state of the sensors and the ability of cells to solubilize or degrade the aggregated proteins. FLuc contains double mutation
    Depositor
    Franz-Ulrich Hartl
    Publication
    Gupta et al Nat Methods. 2011 Sep 4;8(10):879-84. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    FLuc EGFP
    Expression
    Mammalian
    Mutation
    R188Q, R261Q in FLuc
    Available since
    Aug. 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    90172-plasmid-map-sequence-id-226525

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  18. pME-GAL4-ERT2-VP16

    Plasmid
    #82588
    Purpose
    Middle entry vector containing GAL4 fused to ERT2 and the VP16 transcriptional activation domain for tamoxifen-inducible UAS-promoted transgene expression
    Depositor
    Kryn Stankunas
    Publication
    Akerberg et al PLoS One. 2014 Mar 18;9(3):e92217. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GAL4-ERT2-VP16
    Promoter
    None
    Available since
    Dec. 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    82588-plasmid-map-sequence-id-161151

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Showing: 2221 - 2240 of 66152 results