We narrowed to 4,947 results for: mcs

1. pcDNA3 mHIF-1α MYC (P402A/P577A/N813A)

   Plasmid

   #44028

   Depositor

   Celeste Simon

   Article

   Hu et al Mol Biol Cell. 2007 Nov;18(11):4528-42.

   Insert

   mHIF-1α (P402A/P577A/N813A) (Hif1a Mouse)
   

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   P402A, P577A, N813A

   Promoter

   CMV

   Available Since

   March 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pmCherry-MYRIP

   Plasmid

   #89586

   Purpose

   Expression plasmid for a positive control prey protein in NanoSPD 2.0 assays.

   Depositor

   Thomas Friedman

   Article

   Bird et al Mol Biol Cell. 2016 Dec 8. pii: mbc.E16-

   Insert

   MYRIP (Myrip Mouse)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEMS2159

   Plasmid

   #70120

   Purpose

   Promoterless AAV plasmid with MCS to insert promoter for expression of iCre. Contains WPRE.

   Depositor

   Elizabeth Simpson

   Article

   Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

   Insert

   ssAAV-MCS-iCre-WPRE
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   Promoterless

   Available Since

   May 12, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pCMV AURKA-mTurq2

   Plasmid

   #157767

   Purpose

   Expression of AuroraA kinase fused to mTurquoise2 under CMV promoter

   Depositor

   Marc Tramier

   Article

   Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

   Insert

   AURKA (AURKA Human)
   

   Tags

   mTurquoise2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. HA-TEAD1

   Plasmid

   #229411

   Purpose

   Lentiviral or overpexression of cDNA

   Depositor

   Christopher Vakoc

   Article

   Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

   Insert

   TEAD1 (TEAD1 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Promoter

   EFS

   Available Since

   Jan. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHR-SFFV-KRAB-dCas9-P2A-mCherry

   Plasmid

   #60954

   Purpose

   2nd Generation Lentiviral vector. Expresses an N-terminal KRAB-dCas9 fusion protein and mCherry

   Depositor

   Jonathan Weissman

   Article

   Gilbert et al Cell. 2014 Oct 23;159(3):647-61. doi: 10

   Insert

   KRAB-dCas9-P2A-mCherry fusion
   

   Use

   CRISPR and Lentiviral

   Tags

   2x NLS, HA, KRAB domain, and mCherry

   Expression

   Mammalian

   Promoter

   SFFV

   Available Since

   Jan. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJT305 Pa01 Lenti pEF-attB LSR GFP

   Plasmid

   #184948

   Purpose

   Lentiviral delivery of recombinase landing pad

   Depositor

   Ami Bhatt, Lacra Bintu, Patrick Hsu, Michael Bassik

   Article

   Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

   Insert

   Pa01 Lenti pEF1a-attB LSR GFP
   

   Use

   Lentiviral and Synthetic Biology

   Expression

   Mammalian

   Available Since

   Oct. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV hUbC-dCas9-MQ1(Q147L)-EGFP

   Plasmid

   #89793

   Purpose

   Targeted CpG methylation

   Depositor

   Margaret Goodell

   Article

   Lei et al Nat Commun. 2017 Jul 11;8:16026. doi: 10

   Insert

   site-specific DNA-methyltransferase SssI
   

   Use

   CRISPR and Lentiviral

   Tags

   3*FLAG and T2A-EGFP

   Expression

   Mammalian

   Mutation

   Q147L, CAA-->CTA

   Promoter

   hUbC

   Available Since

   July 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUC19/PLIN2-GFP

   Plasmid

   #202194

   Purpose

   knock-in donor vector of GFP to the C-terminus of endogenous human PLIN2

   Depositor

   James Olzmann

   Article

   Roberts et al Dev Cell. 2023 Jul 18:S1534-5807(23)0033

   Insert

   PLIN2 (PLIN2 Human)
   

   Use

   Cloning vector

   Tags

   GFP knock-in

   Promoter

   lac

   Available Since

   Aug. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. FASTHDR-tRNApromo-sgRNA-espCas91_1

    Plasmid

    #167210

    Purpose

    Plasmid for easy cloning of a sgRNA sequence. The Plasmid contain a tRNA promoter to facilitate the expression of any sgRNA sequence and also expresses eSpCas9(1.1)

    Depositor

    Oscar Perez

    Article

    Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pODN-mNG-ANLN

    Plasmid

    #183834

    Purpose

    Repair template for the N-terminal tagging of anillin with mNeonGreen in human cells using CRISPR/Cas9.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Insert

    ANLN homology arms with mNeonGreen-linker (ANLN Human)
    

    Use

    CRISPR; Donor template

    Tags

    mNeonGreen-linker

    Expression

    Mammalian

    Mutation

    Homology arms contain point mutations to remove t…

    Available Since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pU6-sgRNA EF1Alpha-puro-T2A-BFP

    Plasmid

    #60955

    Purpose

    Parental vector for the CRISPRi/a libraries. Expresses an sgRNA from the U6 promoter and a puromycin resistance cassette and BFP from the EF1Alpha promoter

    Depositor

    Jonathan Weissman

    Article

    Gilbert et al Cell. 2014 Oct 23;159(3):647-61. doi: 10

    Inserts

    sgGFP-NT2
    puro-T2A-BFP
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Jan. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1_nV5-AMOTL1

    Plasmid

    #172998

    Purpose

    constitutive expression of V5-tagged AMOTL1

    Depositor

    Michael Wehr

    Article

    Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

    Insert

    V5-AMOTL1 (AMOTL1 Human)
    

    Tags

    V5

    Expression

    Mammalian

    Available Since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLV_hU6-sgRNA_hUbC-dCas9-ZIM3-KRAB-T2a-PuroR

    Plasmid

    #172982

    Purpose

    control & cloning vector for CRISPRi expressing dead Cas9-ZIM3-KRAB fusion

    Depositor

    Michael Wehr

    Article

    Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

    Insert

    control sgRNA
    

    Use

    CRISPR and Lentiviral

    Available Since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. HER2 CA (V659E)

    Plasmid

    #16259

    Depositor

    Mien-Chie Hung

    Article

    Li et al Cancer Cell. 2004 Nov . 6(5):459-69.

    Insert

    HER2 (ERBB2 Human)
    

    Expression

    Mammalian

    Mutation

    Constitutively active: V659E

    Available Since

    Nov. 30, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDNA4.TO-ORF37-2xCSTREP

    Plasmid

    #136198

    Purpose

    Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) ORF37

    Depositor

    Britt Glaunsinger

    Article

    Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

    Insert

    ORF37
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV7.1_3xFLAG-LATS2

    Plasmid

    #172987

    Purpose

    constitutive expression of FLAG-tagged LATS2

    Depositor

    Michael Wehr

    Article

    Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

    Insert

    3xFLAG-LATS2 (LATS2 Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Available Since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGFP-mTet1s pc3901

    Plasmid

    #201700

    Purpose

    Expresses of GFP-tagged mTet1s in mammalian cells

    Depositor

    Cristina Cardoso

    Article

    Arroyo et al Nat Commun. 2022 Sep 2;13(1):5173. doi:

    Insert

    Tet1s (Tet1 Mouse)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    July 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. lentiCRISPR v2-sgControl

    Plasmid

    #125836

    Purpose

    KO control

    Depositor

    Boyi Gan

    Article

    Zhang et al Nat Cell Biol. 2018 Oct;20(10):1181-1192

    Insert

    Control
    

    Use

    Lentiviral

    Available Since

    June 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. MSCV-miR-34b/34c

    Plasmid

    #63933

    Purpose

    MSCV vector for overexpressing mouse miR-34b/34c

    Depositor

    Lin He

    Article

    Okada et al Genes Dev. 2014 Mar 1;28(5):438-50. doi:

    Insert

    microRNA 34b/34c (Mir34c, Mir34b Mouse)
    

    Expression

    Mammalian

    Available Since

    June 19, 2015

    Availability

    Academic Institutions and Nonprofits only