We narrowed to 3,421 results for: mpo

1. pHThMeCP2 (pc3972)

   Plasmid

   #227639

   Purpose

   Mammalian expression vector for human MeCP2 C-terminally tagged to HaloTag.

   Depositor

   Cristina Cardoso

   Article

   Schmidt et al Cells. 2024 Jan 11;13(2):139. doi: 10.33

   Insert

   MeCP2 (MECP2 Human)
   

   Use

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCfB8709

   Plasmid

   #175224

   Purpose

   Xenopus oocyte expression vector containing YOL092W. Contains T7 RNA polymerase binding site, and adds polyA tail to YOL092W. Used for generation of mRNA, for injection into xenopus laevis oocytes.

   Depositor

   Irina Borodina

   Article

   Moller-Hansen et al Front Microbiol. 2024 Apr 12;15:1376653.

   Insert

   YOL092W (CEN.PK strain)
   

   Use

   Mrna expression, injection into xenopus oocytes

   Tags

   PolyA

   Expression

   Mutation

   Promoter

   T7

   Available since

   Aug. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. TLNRD1(4H)-2T_pET47b

   Plasmid

   #224069

   Purpose

   Bacterial expression of the 4-helix bundle of TLNRD1 (residues 143-273) containing the mutations L191T/A225T, fused to an N-terminal hexa-histidine tag. HRV-3C cleavage site

   Depositor

   Ben Goult

   Article

   Ball et al J Cell Biol. 2024 Sep 2;223(9):e20231003

   Insert

   tlnrd1 (TLNRD1 Human)
   

   Use

   Tags

   6xHIS

   Expression

   Bacterial

   Mutation

   L191T/A225T double mutant; reduces affinity of TL…

   Promoter

   T7

   Available since

   Aug. 26, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. TLNRD1(4H)-2E_pET151

   Plasmid

   #224068

   Purpose

   Bacterial expression of the 4-helix bundle of TLNRD1 (residues 143-273) containing the mutations K192E/R233E, fused to an N-terminal hexa-histidine tag. TEV cleavage site

   Depositor

   Ben Goult

   Article

   Ball et al J Cell Biol. 2024 Sep 2;223(9):e20231003

   Insert

   tlnrd1 (TLNRD1 Human)
   

   Use

   Tags

   6xHIS; V5

   Expression

   Bacterial

   Mutation

   K192E/R233E double mutant; prevents actin binding…

   Promoter

   T7

   Available since

   Aug. 26, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. H2B-GFP-INCENP∆cen

   Plasmid

   #184044

   Purpose

   Expresses H2B-GFP fused to INCENP∆cen

   Depositor

   Lienhard Schmitz

   Article

   Pfisterer et al Open Biol. 2024 May;14(5):230460. doi: 1

   Insert

   INCENP (INCENP Human)
   

   Use

   Tags

   GFP and H2B

   Expression

   Mammalian

   Mutation

   Truncated to 1-862

   Promoter

   CMV

   Available since

   June 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-hSyn-DIO-NES-jRGECO1a-WPRE

   Plasmid

   #216276

   Purpose

   AAV vector with hSynapsin promoter and DIO Lox sites for Cre-On expression of red fluorescent calcium indicator jRGECO1a

   Depositor

   Guohong Cui

   Article

   Meng et al Neuron. 2018 May 16;98(4):707-717.e4. do

   Insert

   NES-jRGECO1a
   

   Use

   AAV and Cre/Lox

   Tags

   6XHIS and Xpress

   Expression

   Mutation

   Promoter

   human synapsin 1 (hSyn)

   Available since

   May 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-EGFP-C-IFT46

   Plasmid

   #218724

   Purpose

   Expresses N-terminally EGFP-tagged IFT46 in mammalian cells

   Depositor

   Kazuhisa Nakayama

   Article

   Katoh et al J Biol Chem. 2016 Mar 15. pii: jbc.M116.

   Insert

   IFT46 (IFT46 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   May 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEGFP-C1-IFT25

   Plasmid

   #218721

   Purpose

   Expresses N-terminally EGFP-tagged IFT25 in mammalian cells

   Depositor

   Kazuhisa Nakayama

   Article

   Katoh et al J Biol Chem. 2016 Mar 15. pii: jbc.M116.

   Insert

   IFT25 (HSPB11 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. p2701-AGER-tdTomato

   Plasmid

   #216470

   Purpose

   donor vector for targeting a tdTomato reporter to the human AGER locus at the endogenous ATG start site

   Depositor

   Darrell Kotton

   Article

   Burgess et al Cell Stem Cell. 2024 Apr 15:S1934-5909(2

   Insert

   tdTomato
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. p2707-pHAGE-Ef1aL-dsRed-UBC-tagBFP

    Plasmid

    #216476

    Purpose

    lentiviral dual expression of dsRed and tagBFP

    Depositor

    Darrell Kotton

    Article

    Burgess et al Cell Stem Cell. 2024 Apr 15:S1934-5909(2

    Inserts

    dsRed
    tagBFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1aL and UBC

    Available since

    April 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. OptoProfilin.S138E

    Plasmid

    #208287

    Purpose

    Expresses Cry2(1-531)-mCherry-Profilin.S138E

    Depositor

    Robert Hughes

    Article

    Mann et al Chembiochem. 2024 May 2;25(9):e202400007

    Insert

    Cry2 - mCherry - Profilin.S138E (Cry2, PRF1 Mustard Weed, Mouse)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Ser 138 (sometimes numbered 137) mutated to Glu

    Promoter

    CMV

    Available since

    April 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. OptoProfilin.S138A

    Plasmid

    #208288

    Purpose

    Expresses Cry2(1-531)-mCherry-Profilin.S138A

    Depositor

    Robert Hughes

    Article

    Mann et al Chembiochem. 2024 May 2;25(9):e202400007

    Insert

    Cry2 - mCherry - Profilin (Cry2, PRF1 Mustard Weed, Mouse)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Ser 138 (sometimes numbered 137) mutated to Ala

    Promoter

    CMV

    Available since

    April 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCRISPR-cBEST-v2-SF14P

    Plasmid

    #209448

    Purpose

    improved pCRISPR-cBEST plasmid with optimized sgRNA cloning site and expression of the sgRNA from the SF14P promoter

    Depositor

    Tilmann Weber

    Article

    Maleckis et al ACS Chem Biol. 2024 Feb 10. doi: 10.1021

    Insert

    Codon optimized APOBEC1-nCas9-UGI fusion protein
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    PtipA; sgRNA expression from SF14P promoter

    Available since

    March 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCRISPR-cBEST-v2-kasOP*

    Plasmid

    #209447

    Purpose

    improved pCRISPR-cBEST plasmid with optimized sgRNA cloning site and expression of the sgRNA from the kasOP* promoter

    Depositor

    Tilmann Weber

    Article

    Maleckis et al ACS Chem Biol. 2024 Feb 10. doi: 10.1021

    Insert

    Codon optimized APOBEC1-nCas9-UGI fusion protein
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    PtipA; sgRNA expression from kasOP* promoter

    Available since

    March 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. CCM2_I432D_eGFP

    Plasmid

    #208879

    Purpose

    Express eGFP-CCM2 with the I432D mutation in mammalian cells.

    Depositor

    Guillaume Jacquemet

    Article

    Ball et al J Cell Biol. 2024 Sep 2;223(9):e20231003

    Insert

    CCM2 (CCM2 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    mutation I432D

    Promoter

    Available since

    Dec. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. CCM2_W421AD422A_eGFP

    Plasmid

    #208880

    Purpose

    Express eGFP-CCM2 with the W421A and D422A mutations in mammalian cells.

    Depositor

    Guillaume Jacquemet

    Article

    Ball et al J Cell Biol. 2024 Sep 2;223(9):e20231003

    Insert

    CCM2 (CCM2 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    mutations W421A and D422A

    Promoter

    Available since

    Dec. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. CCM2_I428S_eGFP

    Plasmid

    #208878

    Purpose

    Express eGFP-CCM2 with the I428S mutation in mammalian cells.

    Depositor

    Guillaume Jacquemet

    Article

    Ball et al J Cell Biol. 2024 Sep 2;223(9):e20231003

    Insert

    CCM2 (CCM2 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    mutation I428S

    Promoter

    Available since

    Dec. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pT2/shCol1a1/Scarlet_Seq1.2

    Plasmid

    #201404

    Purpose

    Knockdown of Collagen Type 1 alpha 1. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

    Depositor

    Maria Castro

    Article

    Comba et al Nat Commun. 2022 Jun 24;13(1):3606. doi:

    Insert

    COL1A1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pT2/shCol1a1/Scarlet-Seq1.1

    Plasmid

    #201401

    Purpose

    Knockdown of Collagen Type 1 alpha 1. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

    Depositor

    Maria Castro

    Article

    Comba et al Nat Commun. 2022 Jun 24;13(1):3606. doi:

    Insert

    COL1A1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pT2/Col1a1/GFP4_Seq 1.1

    Plasmid

    #201400

    Purpose

    Knockdown of Collagen Type 1 alpha 1. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

    Depositor

    Maria Castro

    Article

    Comba et al Nat Commun. 2022 Jun 24;13(1):3606. doi:

    Insert

    COL1A1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge