We narrowed to 6,038 results for: WUS;

1. pAAV-CAG-EGFP barcode-5-SV40 polyA

   Plasmid

   #190869

   Purpose

   For barcoded retrograde labeling

   Depositor

   Yuejun Chen

   Article

   Xu et al Elife. 2024 Feb 23;13:e85419. doi: 10.75

   Insert

   EGFP-barcode5
   

   Use

   AAV

   Tags

   Expression

   Mutation

   WT

   Promoter

   Available since

   Nov. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. LV_EF1a_MAPK14-P2A-Hygro_Barcode

   Plasmid

   #170239

   Purpose

   Barcoded lentiviral vector to express MAPK14 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

   Depositor

   Prashant Mali

   Article

   Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

   Insert

   MAPK14 (MAPK14 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pZac2.1 SV40-CMV-SaCas9-3xNLS

   Plasmid

   #78601

   Purpose

   AAV vector containing SaCas9

   Depositor

   Amy Wagers

   Article

   Tabebordbar et al Science. 2016 Jan 22;351(6271):407-11. d

   Insert

   SV40-CMV-SaCas9-3xNLS
   

   Use

   AAV and CRISPR

   Tags

   HA tag

   Expression

   Mammalian

   Mutation

   Promoter

   SV40, CMV promoters

   Available since

   Dec. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

4. pmRFP-N1 human cofilin S3E

   Plasmid

   #50858

   Purpose

   expresses psuedo-phosporylated, non-activatable cofilin fused to mRFP in mammalian cells

   Depositor

   James Bamburg

   Article

   Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

   Insert

   cofilin 1 S3E (CFL1 Human)
   

   Use

   Tags

   mRFP

   Expression

   Mammalian

   Mutation

   S3E psuedo-phosporylated, non-activatable

   Promoter

   CMV

   Available since

   Feb. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pcDNA3-Flag-Tet2 CD

   Plasmid

   #72219

   Purpose

   Express flag-tagged mouse Tet2 catalytic domain.

   Depositor

   Yi Zhang

   Article

   Ito et al Science. 2011 Sep 2;333(6047):1300-3. do

   Insert

   Ten-Eleven Translocation 2 (Tet2 Mouse)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   The plasmid expresses TET2 catalytic domain.

   Promoter

   Available since

   May 1, 2018

   Availability

   Academic Institutions and Nonprofits only

6. pZac2.1 U6-SaDMDR7-U6-SaDMDL2

   Plasmid

   #78603

   Purpose

   AAV vector containing gRNAs (for SaCas9) targeting Dmd introns 22 and 23

   Depositor

   Amy Wagers

   Article

   Tabebordbar et al Science. 2016 Jan 22;351(6271):407-11. d

   Insert

   U6-SaDMDR7-U6-SaDMDL2
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Oct. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

7. PCS2-FlipGFP(Casp3 cleavage seq) T2A mCherry

   Plasmid

   #124431

   Purpose

   Expresses FlipGFP (caspase-3 cleavage sequence) protease reporter and T2A mCherry in zebrafish

   Depositor

   Xiaokun Shu

   Article

   Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

   Insert

   FlipGFP(Casp3 cleavage seq) T2A mCherry
   

   Use

   Zebrafish (if expression level is too high in zeb…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. T7-VEE-CFP

   Plasmid

   #193357

   Purpose

   Self-replicating RNA vector expressing CFP fluorescent protein + PuroR

   Depositor

   Hans Schöler

   Article

   MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

   Insert

   CFP
   

   Use

   Venezuelan equine encephalitis (vee) virus rna re…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   T7

   Available since

   Dec. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Cytosolic Fyn FRET Biosensor

   Plasmid

   #124654

   Purpose

   FRET biosensor. The highly sensitive biosensor has a mutated SH2 domain, ECFP and YPet

   Depositor

   Yingxiao Wang

   Article

   Ouyang et al ACS Sens. 2019 Jan 25;4(1):76-86. doi: 1

   Insert

   Synthetic gene containing ECFP-SH2 domain-substrate-linker-YPet
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. EF1a_KLF4_P2A_Hygro_Barcode

    Plasmid

    #120455

    Purpose

    Barcoded lentiviral vector to express KLF4 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    KLF4 (KLF4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMX-hTAF7

    Plasmid

    #44338

    Depositor

    Marc Timmers

    Article

    Pijnappel et al Nature. 2013 Mar 28;495(7442):516-9. doi

    Insert

    TAF7 (TAF7 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    May 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCA13-pHR-SFFV-HaloTag-FBL

    Plasmid

    #199444

    Purpose

    Expression of HaloTag-FBL in mammalian cells

    Depositor

    Baohui Chen

    Article

    Fu et al J Cell Biol. 2023 Jan 2;222(1):e20220211

    Insert

    FBL (FBL Human)
    

    Use

    Lentiviral

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. CALID

    Plasmid

    #169708

    Purpose

    Cre-activated light-inducible Dre recombinase

    Depositor

    Dali Li

    Article

    Li et al Proc Natl Acad Sci U S A. 2020 Dec 29;11

    Insert

    a double-floxed inverted Dre246-nMag-T2A-pMag-Dre247
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    none

    Promoter

    Available since

    May 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pQCX-CD28 WT

    Plasmid

    #223640

    Purpose

    Retroviral expression of mouse CD28 in mammalian cells

    Depositor

    Enfu Hui

    Article

    Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

    Insert

    CD28 (Cd28 Mouse)
    

    Use

    Retroviral; mammalian expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLVX-hSyn-Galpha-s-IRESWT-Gbetagamma ONE-GO

    Plasmid

    #204342

    Purpose

    GPCR/G protein BRET biosensor

    Depositor

    Mikel Garcia-Marcos

    Article

    Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

    Insert

    hSyn Galpha-s/Gbetagamma ONE-GO
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. MiniCoopR mitfa:KIT L576P

    Plasmid

    #118848

    Purpose

    Expresses human KIT L576P mutant and zebrafish mitfa specifically in zebrafish melanocytes

    Depositor

    Leonard Zon

    Article

    Ablain et al Science. 2018 Nov 1. pii: science.aau650

    Insert

    KIT L576P (KIT Zebrafish, Human)
    

    Use

    Zebrafish expression, tol4

    Tags

    Expression

    Mutation

    KIT L576P

    Promoter

    Available since

    Dec. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCAGGS-FLAG-BMAL1-S513A/S515A/S516A

    Plasmid

    #186835

    Purpose

    Expresses FLAG-BMAL1-S513A/S515A/S516A in mammalian cells

    Depositor

    Hung-Chun Chang

    Article

    Li et al Commun Biol. 2022 Jul 28;5(1):750. doi:

    Insert

    bmal1 (Bmal1 Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed Serine 513/515/516 to Alanine

    Promoter

    Available since

    April 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. aCat DeltaTS (delta570 to 670 aa in aCat TS WT)

    Plasmid

    #101300

    Purpose

    FRET-based tension sensor (TS) technology to develop a probe for molecular-level tension across alphaE-catenin. See Depositor Comments for plasmid construction.

    Depositor

    Alpha Yap

    Article

    Acharya et al Cell Rep. 2017 Mar 21;18(12):2854-2867.

    Insert

    alpha-Catenin
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Contains amino acids 1-509, 670-698, and 699-906 …

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTY-EF1a-mKdm2b (del CxxC domain)-Flag

    Plasmid

    #61740

    Purpose

    Express CxxC domain deleted mouse histone demethylase Kdm2b

    Depositor

    Yi Zhang

    Article

    He et al Nat Cell Biol. 2013 Apr;15(4):373-84. do

    Insert

    Kdm2b (Kdm2b Mouse)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mutation

    delete aa 588-624

    Promoter

    EF1a

    Available since

    Feb. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pCMV-hA3A-BE3-Y130F

    Plasmid

    #113428

    Purpose

    Expresses hA3A-BE3-Y130F in mammalian cells

    Depositor

    Jia Chen

    Article

    Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

    Insert

    hA3A-BE3-Y130F (APOBEC3A S. pyogenes and Bacteriophage PBS2, Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    hAPOBEC3A_Y130F

    Promoter

    CMV

    Available since

    Aug. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pMX-hTAF10

    Plasmid

    #44340

    Depositor

    Marc Timmers

    Article

    Pijnappel et al Nature. 2013 Mar 28;495(7442):516-9. doi

    Insert

    TAF10 (TAF10 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    July 19, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAAV_-CMV-Pcdh15_mini_V4-CD2

    Plasmid

    #199184

    Purpose

    An AAV plasmid designed to facilitate the expression of mini-PCDH15 in the inner ear of a mouse.

    Depositor

    David Corey

    Article

    Ivanchenko et al Nat Commun. 2023 Apr 26;14(1):2400. doi:

    Insert

    mmPcdh15_mini_V4-CD2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    EC4, EC5, EC6, EC7, EC8 are deleted from msPCDH15…

    Promoter

    Available since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET21b-rrt-His8

    Plasmid

    #194088

    Purpose

    Expresses Ec86 reverse transcriptase in Escherichia coli

    Depositor

    Tingting Zou

    Article

    Wang et al Nat Microbiol. 2022 Sep;7(9):1480-1489.

    Insert

    Ec86 rrt
    

    Use

    Tags

    His8

    Expression

    Mutation

    Promoter

    T7

    Available since

    March 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pMX-hTAF11

    Plasmid

    #44341

    Depositor

    Marc Timmers

    Article

    Pijnappel et al Nature. 2013 Mar 28;495(7442):516-9. doi

    Insert

    TAF11 (TAF11 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    Oct. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pMX_hTAF5

    Plasmid

    #44336

    Depositor

    Marc Timmers

    Article

    Pijnappel et al Nature. 2013 Mar 28;495(7442):516-9. doi

    Insert

    TAF5 (TAF5 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    Oct. 31, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pYPQ292 (AaCas12b)-D570A-TV-MS2-VPR

    Plasmid

    #136382

    Purpose

    CRISPRa Gateway entry clone for catalytically dead AaCas12b (D570A) fused with TV activation system, followed by T2A and MCP-VPR fusion protein

    Depositor

    Yiping Qi

    Article

    Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

    Insert

    AaCas12b (D570A)-TV-T2A-MCP-VPR
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    D570A

    Promoter

    Available since

    Feb. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCMV-dCpf1-BE-YEE

    Plasmid

    #107687

    Purpose

    Expresses dLbCpf1-BE-YEE in mammalian cells

    Depositor

    Jia Chen

    Article

    Li et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

    Insert

    dLbCpf1-BE-YEE
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    APOBEC1(W90Y/R126E/R132E), dLbCpf1(D832A/E925A/D1…

    Promoter

    CMV

    Available since

    April 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. EF1a_LHX3_P2A_Hygro_Barcode

    Plasmid

    #120456

    Purpose

    Barcoded lentiviral vector to express LHX3 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    LHX3 (LHX3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. LV_EF1a_MEK1(S218D,S222D)-P2A-Hygro_Barcode

    Plasmid

    #170240

    Purpose

    Barcoded lentiviral vector to express MEK1 (S218D, S222D) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

    Depositor

    Prashant Mali

    Article

    Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

    Insert

    MAP2K1 (MAP2K1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    S218D, S222D

    Promoter

    EF1a

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pET28a-His10-SHP1_PTPase (243-541aa)

    Plasmid

    #180812

    Purpose

    Expression of 10*His tagged human SHP1 phosphatase domain (243-541aa)

    Depositor

    Enfu Hui

    Article

    Xu et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    SHP1_PTPase (243-541aa) (PTPN6 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge