We narrowed to 16,205 results for: She

1. pFastBacMam5_N_Flag_His8_eGFP_3C

   Plasmid

   #178816

   Purpose

   Baculovirus gene transfer into Mammalian cells to product recombinant proteins.

   Depositor

   Kazuhiro Abe

   Type

   Empty backbone

   Use

   Tags

   Flag, His8, eGFP, 3C

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. ecALDH

   Plasmid

   #67921

   Purpose

   Expresses aldehyde dehydrogenase from Escherichia coli, codon optimized for expression in E. coli

   Depositor

   Justin Siegel

   Insert

   Escherichia coli aldehyde dehydrogenase (puuC Escherichia coli)
   

   Use

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFastBacMam6_C_3C_His8_Stop

   Plasmid

   #178810

   Purpose

   Baculovirus gene transfer into Mammalian cells to product recombinant proteins.

   Depositor

   Kazuhiro Abe

   Type

   Empty backbone

   Use

   Tags

   3C, His8, Stop codon

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET-29b(+)_NSP4ss_SerM_NucA-myc

   Plasmid

   #218191

   Purpose

   Bacterial expression of a periplasmically expressed myc-tagged Serratia Marcescens NucA (Benzonase)

   Depositor

   Florence Pojer

   Insert

   nucA
   

   Use

   Tags

   NSP4 signal secretion peptide and myc

   Expression

   Bacterial

   Mutation

   codon optimized ecoli residues - 22-266

   Promoter

   T7

   Available since

   May 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLIX403-EBFP2-ccdB-Puro

   Plasmid

   #158552

   Purpose

   Set of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusions

   Depositor

   Alejandro Chavez

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   EBFP2

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA5-FRT-TO-AID-EGFP

   Plasmid

   #80076

   Purpose

   Mammalian expression vector for C-terminal AID-EGFP tagging

   Depositor

   Andrew Holland

   Insert

   AID-EGFP
   

   Use

   Tags

   AID and EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA5-FRT-TO-EGFP-AID

   Plasmid

   #80075

   Purpose

   Mammalian expression vector for N-terminal EGFP-AID tagging

   Depositor

   Andrew Holland

   Insert

   EGFP-AID
   

   Use

   Tags

   AID and EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pT7-V5-SBP-C1-HshnRNPE1

   Plasmid

   #64921

   Purpose

   Expresses V5- and SBP-tagged hnRNPE1 in mammalian cells

   Depositor

   Elisa Izaurralde

   Insert

   hnRNPE1 (PCBP1 Human)
   

   Use

   Tags

   V5 and SBP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAGIG-6xHIS.Mm.RPE65-eGFP

   Plasmid

   #41019

   Depositor

   John Nickerson

   Insert

   RPE65 (Rpe65 Mouse)
   

   Use

   Tags

   His

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Dec. 13, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. CROPSeq-PP7-p65-HSF

    Plasmid

    #196719

    Purpose

    CROP-Seq library plasmid for CRISPR-A with SAM system (p65-HSF1 activator recruited to PP7 binding loops in the tracrRNA of the guide).

    Depositor

    Bernhard Schmierer

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Expression

    Mutation

    Promoter

    Available since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti-ePEG-LINE1-loxPsym

    Plasmid

    #216866

    Purpose

    Lentiviral guide RNA vector to insert the loxPsym sequence into LINE1 elements

    Depositor

    Leopold Parts

    Insert

    spacerLINE1_cr772scaffold_PBS-loxPsm-HA-tevopreQ
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Aug. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJJR81

    Plasmid

    #75029

    Purpose

    tagBFP2^SEC^3xFlag vector with ccdB markers for cloning homology arms

    Depositor

    Mike Boxem

    Insert

    tagBFP2^SEC^3xFlag
    

    Use

    CRISPR

    Tags

    3xFLAG and C. elegans codon optimized tagBFP2

    Expression

    Worm

    Mutation

    Promoter

    Available since

    June 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLV-NT-3-mCherry

    Plasmid

    #190201

    Purpose

    To express the mouse NT-3 tagged with the FP mCherry

    Depositor

    Pantelis Tsoulfas

    Insert

    NT-3
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    The codon were optimized for better expression

    Promoter

    CMV

    Available since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET His6 TEV cloning vector with BioBrick polycistronic restriction sites (9B)

    Plasmid

    #48284

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAc5.1B-EGFP-DmRRP40

    Plasmid

    #65797

    Purpose

    expresses EGFP-tagged RRP40 in Drosophila cells

    Depositor

    Elisa Izaurralde

    Insert

    RRP40 (Rrp40 Fly)
    

    Use

    Drosophila expression

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    Actin 5C

    Available since

    June 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTre-Tight-luciferase-GFP (467)

    Plasmid

    #65491

    Purpose

    negative control for expression assays

    Depositor

    Roderic Guigo, Rory Johnson

    Insert

    EGFP
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tight TRE promoter

    Available since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJM104: pTrc RFP on pCM66T

    Plasmid

    #74745

    Purpose

    pCM66T with promoter pTrc driving RFP expression (maxRFP = BBa_E1010)

    Depositor

    Mary Lidstrom

    Insert

    RFP (BioBrick part number BBa_E1010)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    pTrc

    Available since

    May 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET3a-His-DJ1

    Plasmid

    #51488

    Purpose

    Inducible expression of human His-DJ1 in E.coli

    Depositor

    Michael J Fox Foundation MJFF

    Insert

    DJ1 (PARK7 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 31, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

19. mouse ptc probe M2-3

    Plasmid

    #58701

    Purpose

    makes mouse ptc probe for in situ hybridization

    Depositor

    Matthew Scott

    Insert

    ptc (Ptch1 Mouse)
    

    Use

    In situ probe

    Tags

    Expression

    Bacterial

    Mutation

    only EcoRI fragment (5' end)

    Promoter

    T7

    Available since

    Aug. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

20. TRIOBP-TwinStrepII

    Plasmid

    #83201

    Purpose

    Expression of tagged protein in mammalian cells

    Depositor

    Peter Barr-Gillespie

    Insert

    TRIOBP (Triobp Mouse)
    

    Use

    Tags

    Twin Strep Tag II

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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21. pKSB- 3xP3-mTurqSV40 GGGG AAGA

    Plasmid

    #174533

    Purpose

    3xP3-mTurquoise fluorescence marker cassette for gene knock-in in mosquitoes

    Depositor

    Eric Marois

    Insert

    3xP3-mTurquoise BsaI cassette
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    3xP3

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pLIX403-EBFP2-ccdB-G418

    Plasmid

    #158556

    Purpose

    Set of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusions

    Depositor

    Alejandro Chavez

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    EBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pExp-PpiB

    Plasmid

    #112572

    Purpose

    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and PpiB (peptidyl-prolyl cis-trans isomerase B from E. coli) fusions with optional C-terminal StrepII-tag

    Depositor

    Marko Hyvönen

    Type

    Empty backbone

    Use

    Tags

    8xHis-PpiB and Strep tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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24. pnos-Fok1:dCas9-nos

    Plasmid

    #62210

    Purpose

    Expresses Fok1:dCas9 under control of nanos promoter and 3'UTR. For germ line restricted high specificity genome engineering in Drosophila melanogaster.

    Depositor

    Simon Bullock

    Insert

    Fok1-dCas9
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    nanos

    Available since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET MBP TEV expression vector with BrioBrick polypromoter restriction sites (14-Q)

    Plasmid

    #48311

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 23, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. MIG-REST

    Plasmid

    #91898

    Purpose

    Expression of mouse REST

    Depositor

    Julien Sage

    Insert

    REST (Rest Mouse)
    

    Use

    Retroviral

    Tags

    ires-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    MSCV

    Available since

    June 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pKT-PDGFRa-IRES-Katushka

    Plasmid

    #133081

    Purpose

    Expresses PDGFRa receptor and katushka fluorescent reporter. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

    Depositor

    Maria Castro

    Insert

    PDGFRa (Pdgfra Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Sperm whale Mb 64Y68F in pVP80K

    Plasmid

    #86956

    Purpose

    Increases expression of sperm whale myoglobin protein for making apomyoglobin as a reagent for measuring rates of hemin dissociation (as opposed to previous construct in pUC).

    Depositor

    John Olson

    Insert

    Mb 64Y68F
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    64Y68F

    Promoter

    T5

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pB-CAG-PE2-NatMX

    Plasmid

    #158567

    Purpose

    Piggybac vector that allows for the generation of PE2-expressing stable cell lines

    Depositor

    Alejandro Chavez

    Insert

    PE2
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. AAVS1_Puro_H2BK_mNG_hSLBP(18-108)

    Plasmid

    #191529

    Purpose

    Donor for targeted integration of mNG_hSLBP(18-108) probe to the AAVS1 safe harbor locus

    Depositor

    Yannick Doyon

    Insert

    H2BK_mNG_hSLBP(18-108)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    mNeonGreen (mNG)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pLIX403-EBFP2-ccdB-Blast

    Plasmid

    #158557

    Purpose

    Set of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusions

    Depositor

    Alejandro Chavez

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    EBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pLXIN2-RFP

    Plasmid

    #99205

    Purpose

    RFP expressing bicistronic retroviral vector

    Depositor

    Saroj Mathupala

    Insert

    Red fluorescent protein
    

    Use

    Retroviral; Bicistronic retroviral expression vec…

    Tags

    Expression

    Mammalian

    Mutation

    The dsRed cDNA was PCR amplified off AddGene plas…

    Promoter

    Available since

    Aug. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pJK615

    Plasmid

    #71699

    Purpose

    Produces E. coli thioredoxin with a C-terminal His6 tag (EcTrxAH6)

    Depositor

    T. J. Kappock

    Insert

    thioredoxin
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pHH0103 NEDD4 WW domain #3

    Plasmid

    #104210

    Purpose

    Bacterial expression of WW domain #3 from NEDD4

    Depositor

    Sachdev Sidhu, Marius Sudol

    Insert

    NEDD4 WW domain #3 (NEDD4 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    codon optimized for expression in bacteria

    Promoter

    tac

    Available since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pHH0103 NEDD4L WW domain #2

    Plasmid

    #104205

    Purpose

    Bacterial expression of WW domain #2 from NEDD4L

    Depositor

    Sachdev Sidhu, Marius Sudol

    Insert

    NEDD4L WW domain #2 (NEDD4L Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    codon optimized for expression in bacteria

    Promoter

    tac

    Available since

    Aug. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pGAL-flp(H305L)-NAT

    Plasmid

    #160380

    Purpose

    overexpression of Flp recombinase step-arrest mutant in yeast

    Depositor

    John McCusker

    Insert

    FLP1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    H305L

    Promoter

    GAL

    Available since

    Dec. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pAG416GAL-EMCV_3C

    Plasmid

    #201941

    Purpose

    Expresses EMCV 3C protease from a GAL promoter with a URA3 marker

    Depositor

    Alejandro Chavez

    Insert

    EMCV 3C protease (EMCVgp1 )
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pUA97

    Plasmid

    #69340

    Purpose

    It encodes a fluorescent protein that can be fused to a gene of interest using SEC CRISPR strategy

    Depositor

    Oliver Hobert

    Insert

    ceBFP SEC 3xFlag
    

    Use

    Tags

    3xFlag and ceBFP (mTagBFP)

    Expression

    Worm

    Mutation

    sqt-1(e1350)

    Promoter

    Available since

    Dec. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pDTA-5

    Plasmid

    #128845

    Purpose

    Gateway-compatible p15A origin plasmid with a DTA negative selection cassette for targeting vector construction, I-CeuI, low copy number

    Depositor

    Mario Capecchi

    Type

    Empty backbone

    Use

    Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pJK633

    Plasmid

    #72397

    Purpose

    Produces Escherichia coli aminoimidazole ribonucleotide synthetase, N-terminal His6 tag (H6EcPurM)

    Depositor

    T. J. Kappock

    Insert

    phosphoribosylaminoimidazole synthetase
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge