We narrowed to 9,280 results for: Pol

Showing: 5601 - 5650 of 9280 results

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pPK1E-1-gfp

Plasmid

#48133

Purpose

Shuttle vector E. coli/B.meg.; gfp-expression under control of RNAP-K1E-inducible promoter

Depositor

Dieter Jahn

Article

Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

Insert

gfp

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 21, 2013

Availability

Academic Institutions and Nonprofits only

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pDECKO-mCherry TFRC_B

Plasmid

#78544

Purpose

Experimental plasmid

Depositor

Roderic Guigo, Rory Johnson

Article

Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

Insert

gRNA1+scaffold+H1promoter+gRNA2

Use

Lentiviral

Expression

Mammalian

Promoter

U6 and U6 (for expressing sgRNA)

Available Since

July 11, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT/TO-UBE2QL1-GFP

Plasmid

#124667

Purpose

Expresses UBE2QL1 with C-terminal GFP tag in mammalian cells

Depositor

Hemmo Meyer

Article

Koerver et al EMBO Rep. 2019 Aug 21:e48014. doi: 10.15

Insert

UBE2QL1 (UBE2QL1 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 GTF2A1

Plasmid

#67076

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

GTF2A1 (GTF2A1 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pRS316-VTC4

Plasmid

#108691

Purpose

VTC4 sequence expressed in yeast plasmid

Depositor

Michael Downey

Article

Bentley-DeSousa et al Cell Rep. 2018 Mar 27;22(13):3427-3439.

Insert

Vacuolar Transporter Chaperone 4

Expression

Yeast

Promoter

VTC4 (native promoter)

Available Since

May 2, 2018

Availability

Academic Institutions and Nonprofits only

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pCDF11-SFPQ(369-598)

Plasmid

#135438

Purpose

Expresses SFPQ 369-598 with TEV-cleavable hexahistidine tag

Depositor

Charles Bond

Article

Lee et al Nucleic Acids Res. 2015 Apr 20;43(7):382

Insert

splicing factor proline and glutamine rich (SFPQ Human)

Tags

Hexahistidine

Expression

Bacterial

Promoter

T7

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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MSCV-PM BRD7 sh1

Plasmid

#41927

Depositor

Stephen Elledge

Article

Burrows et al Proc Natl Acad Sci U S A. 2010 Aug 10;10

Insert

BRD7 (BRD7 Human)

Use

Retroviral

Expression

Mammalian

Available Since

May 10, 2013

Availability

Academic Institutions and Nonprofits only
pCellFree_G03 SMAD2

Plasmid

#67095

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

SMAD2 (SMAD2 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pCellFree_G03 TYK2

Plasmid

#67133

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

TYK2 (TYK2 Human)

Use

Cell-free expression

Tags

EGFP and His

Mutation

A435S

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(+)_UG3

Plasmid

#133874

Purpose

Expresses UG3 in mammalian cells.

Depositor

Sumio Terada

Article

Nakai et al Microscopy (Oxf). 2019 Jul 2. pii: 55252

Insert

UtrCH (UTRN Human)

Tags

helical linker - mEGFP

Expression

Mammalian

Promoter

CMV promoter

Available Since

Jan. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pPK1E-1+t-gfp

Plasmid

#48131

Purpose

Shuttle vector E. coli/B.meg.; gfp-expression under control of RNAP-K1E-inducible promoter

Depositor

Dieter Jahn

Article

Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

Insert

gfp

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 11, 2013

Availability

Academic Institutions and Nonprofits only

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pRK5 GFP RagC (CTD)

Plasmid

#112768

Purpose

express GFP-RagC (C terminal domain only)

Depositor

Roberto Zoncu

Article

Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

Insert

GFP-RagC (C terminal domain only) (RRAGC Human)

Tags

eGFP

Expression

Mammalian

Available Since

Oct. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pRK5 Flag RagB (CTD)

Plasmid

#112769

Purpose

express Flag-RagB (C terminal domain only)

Depositor

Roberto Zoncu

Article

Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

Insert

Flag-RagB (C terminal domain only) (RRAGB Human)

Tags

Flag

Expression

Mammalian

Available Since

Oct. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pRK5 Flag RagB (GD)

Plasmid

#112770

Purpose

express Flag-RagB (G domain only)

Depositor

Roberto Zoncu

Article

Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

Insert

Flag-RagB (G domian only) (RRAGB Human)

Tags

Flag

Expression

Mammalian

Available Since

Oct. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pRK5 GFP RagC (GD)

Plasmid

#112767

Purpose

express GFP-RagC (G domain only)

Depositor

Roberto Zoncu

Article

Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

Insert

GFP-RagC (G domain only) (RRAGC Human)

Tags

eGFP

Expression

Mammalian

Available Since

Oct. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pRK5 Flag RagC (GD)

Plasmid

#112772

Purpose

express Flag-RagC (G domain only)

Depositor

Roberto Zoncu

Article

Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

Insert

Flag-RagC (G domain only) (RRAGC Human)

Tags

Flag

Expression

Mammalian

Available Since

Oct. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pRK5 Flag RagC (CTD)

Plasmid

#112771

Purpose

express Flag-RagC (C terminal domain only)

Depositor

Roberto Zoncu

Article

Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

Insert

Flag-RagC (C terminal domain only) (RRAGC Human)

Tags

Flag

Expression

Mammalian

Available Since

Oct. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pRK5 GFP RagB (CTD)

Plasmid

#112766

Purpose

express GFP-RagB (C terminal domain only)

Depositor

Roberto Zoncu

Article

Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

Insert

GFP-RagB (C terminal domain only) (RRAGB Human)

Tags

eGFP

Expression

Mammalian

Available Since

Oct. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pRK5 GFP RagB (GD)

Plasmid

#112765

Purpose

express GFP-RagB (G domain only)

Depositor

Roberto Zoncu

Article

Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

Insert

GFP-RagB (G domain only) (RRAGB Human)

Tags

eGFP

Expression

Mammalian

Available Since

Oct. 15, 2018

Availability

Academic Institutions and Nonprofits only

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psicheck-2-Kdr

Plasmid

#48178

Purpose

contains Renilla Luciferase gene preceded by an intact (ATG) upstream open reading frame element

Depositor

Vamsi Mootha

Article

Calvo et al Proc Natl Acad Sci U S A. 2009 May 5;106

Insert

5" UTR of 2KDR (MC2R Human)

Use

Luciferase

Available Since

Oct. 16, 2013

Availability

Academic Institutions and Nonprofits only
pDECKO-mCherry MALAT1_Exon.2

Plasmid

#78541

Purpose

Experimental plasmid

Depositor

Roderic Guigo, Rory Johnson

Article

Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

Insert

gRNA1+scaffold+H1promoter+gRNA2

Use

Lentiviral

Expression

Mammalian

Promoter

U6 (for expressing sgRNA) and U6 promoter

Available Since

July 11, 2016

Availability

Academic Institutions and Nonprofits only

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pDECKO-mCherry MALAT1_Exon.4

Plasmid

#78543

Purpose

Experimental plasmid

Depositor

Roderic Guigo, Rory Johnson

Article

Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

Insert

gRNA1+scaffold+H1promoter+gRNA2

Use

Lentiviral

Expression

Mammalian

Promoter

U6 and U6 (for expressing sgRNA)

Available Since

July 11, 2016

Availability

Academic Institutions and Nonprofits only

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pScalps_puro_mklf1

Plasmid

#59918

Purpose

Expresses mouse Kruppel-like factor1

Depositor

Silvia Monticelli

Article

Deho' et al J Immunol. 2014 Sep 1;193(5):2196-206. d

Insert

Kruppel-like factor 1 (Klf1 Mouse)

Use

Lentiviral

Available Since

Oct. 3, 2014

Availability

Academic Institutions and Nonprofits only
p5M-PR-B-1-688

Plasmid

#89115

Purpose

mammalian expression of human progesterone receptor amino acid residues 1-688 in pCMV5 with 5 restriction sites deleted in polylinker

Depositor

Elizabeth Wilson

Article

Liu et al J Biol Chem. 2011 Aug 26;286(34):29951-6

Insert

human progesterone receptor-B N-term+DNA binding domain (PGR Human)

Expression

Mammalian

Mutation

5 restriction sites deleted in polylinker, contai…

Promoter

CMV

Available Since

April 21, 2017

Availability

Academic Institutions and Nonprofits only

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GST-PC-2-CTS

Plasmid

#86068

Purpose

A bacterial expression plasmid encoding polycystin-2 ciliary targeting signal with N-terminus GST-tag.

Depositor

Lei Lu

Article

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Insert

Polycystin-2 (Pkd2 Human)

Tags

GST

Expression

Bacterial

Mutation

contains the region from 1-15

Promoter

tac

Available Since

May 26, 2017

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 BMPR2

Plasmid

#67127

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

BMPR2 (BMPR2 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
MSCV-PM BRD7 sh3

Plasmid

#41930

Depositor

Stephen Elledge

Article

Burrows et al Proc Natl Acad Sci U S A. 2010 Aug 10;10

Insert

BRD7 (BRD7 Human)

Use

Retroviral

Expression

Mammalian

Available Since

May 10, 2013

Availability

Academic Institutions and Nonprofits only
MSCV-PM BRD7 sh2

Plasmid

#41928

Depositor

Stephen Elledge

Article

Burrows et al Proc Natl Acad Sci U S A. 2010 Aug 10;10

Insert

BRD7 (BRD7 Human)

Use

Retroviral

Expression

Mammalian

Available Since

May 10, 2013

Availability

Academic Institutions and Nonprofits only
pPK1E-2+t-gfp

Plasmid

#48132

Purpose

Shuttle vector E. coli/B.meg.; gfp-expression under control of RNAP-K1E-inducible promoter

Depositor

Dieter Jahn

Article

Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

Insert

gfp

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 21, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
DCT.VV.Orf164

Plasmid

#12852

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf164

Expression

Mammalian

Available Since

Dec. 13, 2006

Availability

Academic Institutions and Nonprofits only
FLAG-ELL 1-373 pBacPAK8

Plasmid

#15328

Depositor

Joan Conaway, Ronald Conaway

Article

Kong et al Proc Natl Acad Sci U S A. 2005 Jul 19. 1

Insert

eleven nineteen lysine rich in leukemia (ELL Human)

Use

Baculovirus construction

Tags

FLAG

Mutation

Amino acids 1-373

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
FLAG-ELL 45-621 pBacPAK8

Plasmid

#15329

Depositor

Joan Conaway, Ronald Conaway

Article

Kong et al Proc Natl Acad Sci U S A. 2005 Jul 19. 1

Insert

eleven nineteen lysine rich in leukemia (ELL Human)

Use

Baculovirus construction

Tags

FLAG

Mutation

Amino acids 45-621

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
FLAG-ELL 45-373 pBacPAK8

Plasmid

#15330

Depositor

Joan Conaway, Ronald Conaway

Article

Kong et al Proc Natl Acad Sci U S A. 2005 Jul 19. 1

Insert

eleven nineteen lysine rich in leukemia (ELL Human)

Use

Baculovirus construction

Tags

FLAG

Mutation

Amino acids 45-373

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
FLAG ELL 500-621 pBacPAK8

Plasmid

#15331

Depositor

Joan Conaway, Ronald Conaway

Article

Kong et al Proc Natl Acad Sci U S A. 2005 Jul 19. 1

Insert

eleven nineteen lysine rich in leukemia (ELL Human)

Use

Baculovirus construction

Tags

FLAG

Mutation

Amino acids 500-621 only

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
pLVX-EF1_-FCRL3-3xFLAG-puro

Plasmid

#248155

Purpose

Lentiviral vector expressing human FCRL3 with 3xFLAG tags

Depositor

Silvia Monticelli

Article

Bianchi et al J Exp Med (2026) 223 (1): e20242474.

Insert

FCRL3 (FCRL3 Human)

Use

Lentiviral

Tags

3x FLAG

Mutation

A 3xFLAG epitope tag is fused in frame to the C-t…

Promoter

EF1_

Available Since

Dec. 12, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEM-PAmCherry-RPA194

Plasmid

#247354

Purpose

CRISPR/Cas9 donor plasmid to tag human RPA194 with PAmCherry

Depositor

Kazuhiro Maeshima

Article

Ide et al Sci Adv. 2020 Oct 14;6(42). pii: 6/42/ea

Insert

RPA194 (POLR1A) (POLR1A Human)

Use

CRISPR

Tags

PAmCherry

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEM-mAID-RPA194

Plasmid

#247355

Purpose

CRISPR/Cas9 donor plasmid to tag human RPA194 with mAID

Depositor

Kazuhiro Maeshima

Article

Ide et al Sci Adv. 2020 Oct 14;6(42). pii: 6/42/ea

Insert

RPA194 (POLR1A) (POLR1A Human)

Use

CRISPR

Tags

mAID

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pDOXOFF (STOP)99xGly

Plasmid

#211349

Purpose

DOX-regulated lentiviral expression of 99xGly-EGFP-3xHA lacking initiation codon

Depositor

Raghu Chivukula

Article

Yang et al Science. 2025 Jul 17;389(6757):eado2403.

Insert

(STOP)99xGly-EGFP-3xHA

Use

Lentiviral

Tags

EGFP-3xHA

Mutation

Start (ATG) codon replaced with stop (TAG) codon

Available Since

Oct. 28, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pTB105

Plasmid

#236778

Purpose

Backbone for cloning sgRNAs to be used with CRISPR Cas9 cytosine base editor. Expressed from the 18S rRNA SSU locus in Leishmania. Contains puromycin selection marker.

Depositor

Tom Beneke

Article

Herrmann May et al eLife. 2025 Feb 24;13:RP97437. doi: 10.7

Type

Empty backbone

Use

CRISPR

Available Since

Oct. 23, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
huCofilin S3A SSR

Plasmid

#245939

Purpose

Expresses human cofilin with mutation S3A that prevents cofilin's inactivation by phosphorylation of S3

Depositor

James Bamburg

Article

Agnew et al J Biol Chem. 1995 Jul 21;270(29):17582-7

Insert

cofilin 1 (cfl1) (CFL1 Human)

Expression

Mammalian

Mutation

S3A;silent mutations (NTs 67 to 75) in which TCTT…

Promoter

CMV

Available Since

Oct. 22, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-LSL-Cyp26b1-PGK-H2BRFP

Plasmid

#241862

Purpose

Cre-mediated Cyp26b1 overexpression

Depositor

Elaine Fuchs

Article

Tierney et al Science. 2024 Mar 8;383(6687):eadi7342.

Insert

Cyp26b1 (Cyp26b1 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pDonr201_PARP1

Plasmid

#240317

Purpose

Entry vector for Gateway with PARP1

Depositor

Junjie Chen

Article

Nie et al Elife. 2024 Apr 5;12:RP89303. doi: 10.75

Insert

PARP1 (PARP1 Human)

Expression

Bacterial

Available Since

Oct. 9, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK5R DD-FAM98B

Plasmid

#211357

Purpose

Transient expression of DD-V5-FAM98B

Depositor

Raghu Chivukula

Article

Yang et al Science. 2025 Jul 17;389(6757):eado2403.

Insert

DD-FAM98B (FAM98B Human)

Tags

DD-V5

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-EF1A-PARP14-1535-1601-Hygro

Plasmid

#238100

Purpose

Expression of structurally predicted WWE domain of PARP14 with EGFP on C terminal

Depositor

Robert Sobol

Article

Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

Insert

PARP14 (PARP14 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

aa 1535-1601 only

Promoter

EF1alpha

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-Hygro-EF1A-DTX2(8-97)-EGFP

Plasmid

#196230

Purpose

EGFP fused to the C-terminus of first WWE domain within Deltex2 & a hygromycin resistance cassette

Depositor

Robert Sobol

Article

Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

Insert

C-terminal EGFP tagged DTX2(8-97)

Use

Lentiviral

Tags

EGFP and Myc tag

Expression

Mammalian

Promoter

EF1A

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-Hygro-EF1A-Af1521(WT)-EGFP

Plasmid

#196233

Purpose

EGFP fused to the C-terminus of Af1521 (WT) macrodomain & a hygromycin resistance cassette

Depositor

Robert Sobol

Article

Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

Insert

C-terminal EGFP tagged Af1521(WT)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

EF1A

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-Hygro-EF1A-Af1521(G42E)-myc

Plasmid

#196238

Purpose

Af1521(G42E) macrodomain with a myc tag fused to the C terminus & a hygromycin resistance cassette

Depositor

Robert Sobol

Article

Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

Insert

Af1521(G42E)-myc

Use

Lentiviral

Tags

myc tag

Expression

Mammalian

Promoter

EF1A

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-Hygro-EF1A-RNF146(92-168)-EGFP

Plasmid

#196228

Purpose

EGFP fused to the C-terminus of a WWE domain within RNF146 & a hygromycin resistance cassette

Depositor

Robert Sobol

Article

Al-Rahahleh et al DNA Repair (Amst). 2025 Jun;150:103845.

Insert

RNF146(92-168)-EGFP

Use

Lentiviral

Tags

EGFP and Myc tag

Expression

Mammalian

Promoter

EF1A

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pGS-BacA-21232

Plasmid

#109177

Purpose

Plasmid for expression of C-terminally CBP-3xFLAG-ProteinA-tagged proteins under the control of a polh promoter in baculovirus/insect cell systems

Depositor

Monika Golas

Article

Golas et al Mol Biotechnol. 2018 Nov;60(11):820-832.

Type

Empty backbone

Tags

CBP-3xFLAG-ProteinA

Expression

Insect

Promoter

polyhedrin

Available Since

July 29, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
FUS LC 8Y->S

Plasmid

#226606

Purpose

8 tyrosines to serine

Depositor

Nicolas Fawzi

Article

Wake et al Nat Chem Biol. 2025 Jul;21(7):1076-1088.

Insert

FUS LC 8Y->S (FUS Human)

Tags

6His

Expression

Bacterial

Mutation

8 tyrosines to serine

Promoter

T7

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge

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We narrowed to 9,280 results for: Pol