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pAGM65905

Plasmid

#153217

Purpose

Level 2 cloning vector for one or several guide RNAs, already contains Cas9 and a kanamycin selection cassette for plant transformation

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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Nicastrin-CD4d3+4-bio

Plasmid

#73106

Purpose

EXPRESs plasmid for human erythrocyte surface proteins encoding Nicastrin with rat CD4d3+4-bio

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

Nicastrin (NCSTN Human)

Tags

biotinylation peptide and ratCD4d3+4

Expression

Mammalian

Promoter

CMV

Available Since

March 28, 2016

Availability

Academic Institutions and Nonprofits only

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pQCXIP-LIP

Plasmid

#15714

Depositor

Joan Massague

Article

Gomis et al Cancer Cell. 2006 Sep . 10(3):203-14.

Insert

LIP (CEBPB Human)

Use

Retroviral

Expression

Mammalian

Available Since

Sept. 7, 2007

Availability

Academic Institutions and Nonprofits only
IFNGR2_pCSdest

Plasmid

#53823

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

IFNGR2 (IFNGR2 Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits
pcDNA3.1(+) Drp1 V2 S579D

Plasmid

#46343

Depositor

David Chan

Article

Loson et al Mol Biol Cell. 2013 Jan 2.

Insert

Drp1 V2 (Dnm1l Mouse)

Expression

Mammalian

Mutation

S579D

Promoter

CMV

Available Since

July 23, 2014

Availability

Academic Institutions and Nonprofits only
pUAST-GFP-RpL10Ab

Plasmid

#71729

Purpose

Expression of Drosophila melanogaster Rpl10Ab-GFP fusion

Depositor

Herman Dierick

Article

Thomas et al PLoS One. 2012;7(7):e40276. doi: 10.1371

Insert

RpL10Ab (RpL10Ab Fly)

Tags

EGFP

Expression

Insect

Promoter

hsp70

Available Since

Dec. 2, 2015

Availability

Academic Institutions and Nonprofits only
pLVXPuro-epsilon-COP-GFP

Plasmid

#66604

Purpose

Expresses epsilon COP as a GFP fusion

Depositor

David Stephens

Article

Morohashi et al Biochem J. 2010 Apr 14;427(3):401-12. do

Insert

epsilon COP (COPE Bovine)

Use

Lentiviral

Expression

Mammalian

Available Since

July 28, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(+) Drp1 V2 S579A

Plasmid

#46342

Depositor

David Chan

Article

Loson et al Mol Biol Cell. 2013 Jan 2.

Insert

Drp1 V2 (Dnm1l Mouse)

Expression

Mammalian

Mutation

S579A

Promoter

CMV

Available Since

July 23, 2014

Availability

Academic Institutions and Nonprofits only
pET14b PHAS-I

Plasmid

#15679

Depositor

John Lawrence

Article

Choi et al J Biol Chem. 2003 May 30. 278(22):19667-

Insert

PHAS-I (Eif4ebp1 Rat)

Tags

6xHis

Expression

Bacterial

Mutation

Nucleotides 1-354 of rat PHAS-I plus 450bp of 3&#…

Available Since

Sept. 24, 2007

Availability

Academic Institutions and Nonprofits only
GFP-GW182delta1

Plasmid

#11592

Depositor

Edward Chan

Article

Jakymiw et al Nat Cell Biol. 2005 Dec . 7(12):1267-74.

Insert

GW182 (TNRC6A Human)

Tags

GFP

Expression

Mammalian

Mutation

contains only aa1-498; SEE DEPOSITOR NOTES BELOW

Available Since

June 30, 2006

Availability

Academic Institutions and Nonprofits only
pJAF215

Plasmid

#52402

Purpose

Expresses Flag-tagged mouse Arf1

Depositor

Gregory Pazour

Article

Follit et al PLoS Genet. 2014 Feb 20;10(2):e1004170.

Insert

Arf1 (Arf1 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

April 14, 2014

Availability

Academic Institutions and Nonprofits only
pET28_human-pro-IL-18_DeltaN24

Plasmid

#214327

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

IL18 (IL18 Human)

Tags

His-TEV

Expression

Bacterial

Mutation

Deletion of first 24 amino acids

Promoter

T7

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pFastBac_human-pro-IL-18

Plasmid

#214337

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

IL18 (IL18 Human)

Tags

His-TEV

Expression

Bacterial

Promoter

T7

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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Ntn4-AP-His

Plasmid

#71980

Purpose

Expresses the entire Netrin 4 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Ntn4 (Ntn4 Mouse)

Tags

AP-His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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FLAG-FLJ90652

Plasmid

#15359

Depositor

Joan Conaway, Ronald Conaway

Article

Jin et al J Biol Chem. 2005 Dec 16. 280(50):41207-

Insert

FLJ90652 (INO80E Human)

Tags

FLAG

Expression

Mammalian

Available Since

Aug. 17, 2007

Availability

Academic Institutions and Nonprofits only

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mCHOP10.pCDNA1

Plasmid

#21899

Depositor

David Ron

Article

Ron et al Genes Dev. 1992 Mar;6(3):439-53. doi: 10

Insert

CHOP10 (Ddit3 Mouse)

Expression

Mammalian

Available Since

Sept. 10, 2009

Availability

Academic Institutions and Nonprofits only
pcDNA3-HA-ERK5kin

Plasmid

#65245

Purpose

Expression of HA tagged ERK5 kinase domain in mammalian cells

Depositor

Axel Ullrich

Article

Buschbeck et al J Biol Chem. 2002 Aug 16;277(33):29503-9

Insert

ERK5kin (MAPK7 Human)

Tags

HA

Expression

Mammalian

Mutation

deleted AA 410-817

Promoter

CMV

Available Since

Nov. 16, 2016

Availability

Academic Institutions and Nonprofits only
pARF1-YFP

Plasmid

#11390

Depositor

Joel Swanson

Article

Beemiller et al PLoS Biol. 2006 May 9. 4(6):e162.

Insert

ADP Ribosylation Factor 1 (ARF1 Human)

Tags

YFP

Expression

Mammalian

Available Since

May 30, 2006

Availability

Academic Institutions and Nonprofits only

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pTS1051-Tier1-PhCMV-4xCox8

Plasmid

#169490

Purpose

Tier-1 vector encoding PhCMV-driven four-fold repeat of a mitochondrial matrix localization signal derived from mitochondrial matrix protein Cox8 (PhCMV-4xCox8-pA)

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven mitochondrial localization signal from cytochrome c oxidase subunit 8

Expression

Mammalian

Promoter

PhCMV

Available Since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2 mouse CV2 PC

Plasmid

#25776

Depositor

Edward De Robertis

Article

Coffinier et al Mech Dev. 2002 Dec;119 Suppl 1(Suppl 1):

Insert

cross veinless 2 (Bmper Mouse)

Tags

PC

Expression

Mammalian

Available Since

July 2, 2010

Availability

Academic Institutions and Nonprofits only

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