We narrowed to 13,238 results for: BASE

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pRDA_085

Plasmid

#158583

Purpose

Lentiviral expression of SpCas9; P2A site provides mKate2

Depositor

John Doench, David Root

Article

Hanna et al Cell. 2021 Feb 18;184(4):1064-1080.e20.

Insert

SpCas9

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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ND4 right TALE–G1333 DddAtox N

Plasmid

#158096

Purpose

expresses the right-side ND4 TALE–DddAtox half in mammalian cells

Depositor

David Liu

Article

Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

Insert

COX8A MTS–3xFLAG–ND4 right TALE–G1333 DddA tox-N–1xUGI

Expression

Mammalian

Mutation

WT

Available Since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-hU6-mU6-Cre2GO

Plasmid

#136907

Purpose

Lentivirus for base editing activatable Cre recombinase expression in mammalian cells. All in one vector with sgCre2GO.

Depositor

Lukas Dow

Article

Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

Insert

BlasCyGO

Use

Lentiviral

Mutation

ATG>ACG

Promoter

SFFV

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pBT362

Plasmid

#127844

Purpose

clone BE4max-NG CBEs by SapI Golden Gate

Depositor

David Liu

Article

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

BE4max-NG without deaminase

Available Since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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mCh-KIF1A*-strep

Plasmid

#120165

Purpose

Expresses a chimera of mCherry (fluorescent tag), a KIF1A-based anterograde motor and streptavidin

Depositor

Juan Bonifacino

Article

Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

Insert

kinesin family member 1A (Kif1a Mouse)

Tags

Streptavidin and mCherry

Expression

Mammalian

Mutation

Truncated KIF1A: 1-405 aa

Promoter

CMV

Available Since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

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Strep-KIFC1*-HA

Plasmid

#120171

Purpose

Expresses a chimera of streptavidin, a KIFC1-based retrograde motor and HA tag

Depositor

Juan Bonifacino

Article

Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

Insert

kinesin family member C1 (KIFC1 Human)

Tags

HA and Streptavidin

Expression

Mammalian

Mutation

Truncated KIFC1: 125-673 aa

Promoter

CMV

Available Since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

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pDV02_linear_entry_TagBFP-2A-MCS-2A-mNeonGreen

Plasmid

#200401

Purpose

Entry vector for open-loop ADAR-based RNA sensors. Expresses TagBFP constitutively. Expresses mNeonGreen upon target detection

Depositor

James Collins

Article

Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

Insert

Open-loop DART VADAR sensor entry cassette

Use

Synthetic Biology

Expression

Mammalian

Available Since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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pSCBE3-NG

Plasmid

#218159

Purpose

This plasmid harbors the base editor SCBE3-NG along with an sgRNA cloning cassette, facilitating cytosine base editing at targets bearing NG PAM in Streptomyces.

Depositor

Yuhui Sun

Article

Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:

Inserts

scbe3-NG
a programmable sgRNA cloning cassette

Use

CRISPR; Sgrna transcription

Expression

Bacterial

Mutation

Mutations in the portion of SpCas9 : D10A / L1111…

Promoter

rpsL promoter

Available Since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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peSCBE3-NG-HF1

Plasmid

#218164

Purpose

This plasmid harbors the base editor eSCBE3-NG-HF1 along with an sgRNA cloning cassette, facilitating high-efficiency and high-fidelity cytosine base editing at targets bearing NG PAM in Streptomyces.

Depositor

Yuhui Sun

Article

Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:

Inserts

escbe3-NG-HF1
a programmable sgRNA cloning cassette

Use

CRISPR; Sgrna transcription

Expression

Bacterial

Mutation

Mutation in the portion of deaminase hAPOBEC3A : …

Promoter

rpsL promoter

Available Since

May 13, 2024

Availability

Academic Institutions and Nonprofits only

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G1333 DddAtox-C–SaKKH-Cas9(D10A)

Plasmid

#157838

Purpose

expresses split DddAtox-Cas9 construct in mammalian cells

Depositor

David Liu

Article

Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

Insert

G1333 DddAtox-C–SaKKH-Cas9(D10A)–UGI–SV40 NLS

Expression

Mammalian

Available Since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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G1333 DddAtox-N–SaKKH-Cas9(D10A)

Plasmid

#157837

Purpose

expresses split DddAtox-Cas9 construct in mammalian cells

Depositor

David Liu

Article

Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

Insert

G1333 DddAtox-N–SaKKH-Cas9(D10A)–UGI–SV40 NLS

Expression

Mammalian

Available Since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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R26-EGFP MMEJ donor vector

Plasmid

#137926

Purpose

R0SA26 knock-in vector with microhomology-mediated end-joining(MMEJ)-based method

Depositor

Hiroshi Kiyonari

Article

Abe et al Cell Rep. 2020 May 19;31(7):107653. doi:

Insert

SA-EGFP-bpA with micro-homology arms

Use

Mouse Targeting

Available Since

May 19, 2020

Availability

Academic Institutions and Nonprofits only

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CP1300-ABEmax

Plasmid

#119810

Purpose

A-to-G base editor

Depositor

David Liu

Article

Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

Insert

ecTadA-ecTadA*(7.10)-nSpCas9 (CP1300)

Expression

Mammalian

Available Since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

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CP1249-CBEmax

Plasmid

#119804

Purpose

C-to-T base editor

Depositor

David Liu

Article

Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

Insert

rApobec1-nSpCas9 (CP1249) -UGI-UGI

Expression

Mammalian

Available Since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

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pJY-RpABE-PDS3_IMS

Plasmid

#112875

Purpose

binary vector for IMS (Induced Mis-Splicing) of PDS3 in Arabidopsis

Depositor

Jin-Soo Kim, Jae-Young Yun

Article

Kang et al Nat Plants. 2018 Jun 4. pii: 10.1038/s41

Inserts

RPS5A promoter
ABE7.10
U6-sgRNA targeting PDS3

Use

CRISPR

Expression

Plant

Available Since

Sept. 12, 2018

Availability

Academic Institutions and Nonprofits only

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LRT2B-GO2

Plasmid

#136909

Purpose

Lentivirus expressing GO2 sgRNA

Depositor

Lukas Dow

Article

Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

Insert

sgGO2

Use

Lentiviral

Mutation

WT

Promoter

hU6

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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Lenti AID-BEmax

Plasmid

#157950

Purpose

Lentiviral expression of AID-BEmax

Depositor

Dali Li

Article

Zhang et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

Insert

Lenti AID-BEmax

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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p663-UBC-TurboID-V5-eGFP_IDG-K

Plasmid

#135264

Purpose

Gateway destination clone of eGFP (as control) tagged with N-terminal TurboID-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

TurboID-V5-eGFP

Use

Lentiviral; Gateway destination

Tags

V5-TurboID

Expression

Mammalian

Promoter

Ubiquitin

Available Since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pFA6a-6xGLY-Myc-HIS3MX6

Plasmid

#20768

Depositor

Mark Hochstrasser

Article

Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

Type

Empty backbone

Use

Pcr-based c-terminal tagging

Expression

Yeast

Available Since

March 27, 2009

Availability

Academic Institutions and Nonprofits only

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SpCas9-CBE6d-IVT

Plasmid

#215836

Purpose

Template for in vitro transcription of CBE6d (with SpCas9)

Depositor

David Liu

Article

Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

Insert

CBE6d-SpCas9-2xUGI

Use

In vitro transcription template

Available Since

March 8, 2024

Availability

Academic Institutions and Nonprofits only

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