We narrowed to 15,732 results for: LEA

1. pcDNA3-mCherry-TSC2(AA,3Q)

   Plasmid

   #192444

   Purpose

   Encodes mCherry-tagged TSC2 isoform 5 with S939A, T1439A, K1614Q, K1615Q, R1616Q mutations to disrupt Akt phosphorylation and GAP activity.

   Depositor

   Jin Zhang

   Article

   Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1

   Insert

   mCherry-TSC2(S939A,T1439A,K1614Q,K1615Q,R1616Q ) (TSC2 Human)
   

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   TSC2 Ser 939 and Thr 1439 (equivalent to 1462 in …

   Promoter

   CMV

   Available Since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTRIP-SFFV-mtagBFP-2A RIGI K270A

   Plasmid

   #167290

   Purpose

   Lentiviral expression construct encoding a TagBFP in frame with a self-cleaving catalytically dead (K270A) RLR sensor RIG-I

   Depositor

   Agnel Sfeir

   Article

   Tigano et al Nature. 2021 Mar;591(7850):477-481. doi:

   Insert

   DDX58 (RIGI Human)
   

   Use

   Lentiviral

   Tags

   tagBFP

   Expression

   Mammalian

   Mutation

   K270A

   Promoter

   SFFV

   Available Since

   April 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-myc3-Mdm2 D367E

   Plasmid

   #52057

   Purpose

   expression of non-cleavable Mdm2 in mammalian cells

   Depositor

   Tyler Jacks, Trudy Oliver

   Article

   Oliver et al Mol Cell. 2011 Jul 8;43(1):57-71. doi: 1

   Insert

   Mdm2 D367E (MDM2 Human)
   

   Tags

   myc3

   Expression

   Mammalian

   Mutation

   D367E

   Promoter

   CMV

   Available Since

   March 13, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pMT-MCS-NLS-BirA-2A-mCherry_Ras

   Plasmid

   #80059

   Purpose

   MCS for cloning promoter to drive HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

   Depositor

   Tatjana Sauka-Spengler

   Article

   Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

   Insert

   Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
   

   Use

   Zebrafish biotagging

   Tags

   3x HA

   Expression

   Bacterial

   Available Since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. TTR C10S S85C F87E

   Plasmid

   #238074

   Purpose

   Bacterial expression of human TTR mutants

   Depositor

   Peter Wright

   Article

   Leach et al J Mol Biol. 2024 Aug 15;436(16):168673.

   Insert

   TTR (TTR Human)
   

   Expression

   Bacterial

   Mutation

   C10S S85C F87E

   Promoter

   T7

   Available Since

   June 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMBS-864

   Plasmid

   #218018

   Purpose

   GST-tag fused to TEV Protease cleavage site for protein purification and cleavage

   Depositor

   Michael Schroda

   Article

   Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

   Insert

   GST-tag
   

   Use

   Synthetic Biology

   Tags

   TEV protease cleavage site

   Available Since

   Aug. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMBS-848

   Plasmid

   #218003

   Purpose

   GST-tag fused to PreScission Protease cleavage site for protein purification and cleavage

   Depositor

   Michael Schroda

   Article

   Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

   Insert

   GST-tag
   

   Use

   Synthetic Biology

   Tags

   PreScission protease cleavage site

   Available Since

   Aug. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMBS-849

   Plasmid

   #218004

   Purpose

   8xHis-tag fused to PreScission Protease cleavage site for protein purification and cleavage

   Depositor

   Michael Schroda

   Article

   Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

   Insert

   8xHis-tag
   

   Use

   Synthetic Biology

   Tags

   PreScission protease cleavage site

   Available Since

   Aug. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. hPCNA-C148S

   Plasmid

   #190939

   Purpose

   untagged human PCNA with a cysteine to serine mutation

   Depositor

   Brian Kelch

   Article

   Magrino et al J Biol Chem. 2023 May;299(5):104656. doi

   Insert

   Proliferating Cell Nuclear Antigen (PCNA Human)
   

   Expression

   Bacterial

   Mutation

   changed cysteine 148 to serine

   Promoter

   T7

   Available Since

   March 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. SYKA-c022

    Plasmid

    #175490

    Purpose

    Protein expression in bacterial cells. Tandem SH2 domains, M6-N269. Can be biotinylated.

    Depositor

    Opher Gileadi

    Article

    Bashore et al bioRxiv. 2023 Jul 29:2023.07.28.551026.

    Insert

    SYK (SYK Human)
    

    Tags

    AviTag and His6-TEV

    Expression

    Bacterial

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. MSNA-c028

    Plasmid

    #175470

    Purpose

    Protein expression in bacterial cells. FERM domain, M1-E346. Contains H288A point mutation that reduces binding to CD44.

    Depositor

    Opher Gileadi

    Article

    Du et al J Biol Chem. 2023 Oct 20:105382. doi: 10

    Insert

    MSN (MSN Human)
    

    Tags

    His6-TEV

    Expression

    Bacterial

    Mutation

    H288A

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. MSNA-c021

    Plasmid

    #175468

    Purpose

    Protein expression in bacterial cells. FERM domain, M1-E346. Contains L281R point mutation that reduces binding to CD44.

    Depositor

    Opher Gileadi

    Article

    Du et al J Biol Chem. 2023 Oct 20:105382. doi: 10

    Insert

    MSN (MSN Human)
    

    Tags

    His6-TEV

    Expression

    Bacterial

    Mutation

    L281R

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. MSNA-c001

    Plasmid

    #175466

    Purpose

    Protein expression in bacterial cells. FERM domain, M1-E346. Used for crystallography (PDB:6TXQ).

    Depositor

    Opher Gileadi

    Article

    Du et al J Biol Chem. 2023 Oct 20:105382. doi: 10

    Insert

    MSN (MSN Human)
    

    Tags

    His6-TEV

    Expression

    Bacterial

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. 3xEGFP-mMeikin(1-391) - hRad21(142-275, E169R, R172E) - mMeikin(387-434)

    Plasmid

    #174713

    Purpose

    Mammalian expression of mouse Meikin with inserted C-terminal Separase cleavage site inactivated

    Depositor

    Iain Cheeseman

    Article

    Maier et al Dev Cell. 2021 Aug 9;56(15):2192-2206.e8

    Insert

    Meikin (Meikin Mouse)
    

    Tags

    3xEGFP

    Expression

    Mammalian

    Mutation

    mMeikin: 1-391; hRad21: 142-275, E169R, R172E; mM…

    Available Since

    Sept. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3HA-AMOT p130∆PPxY1(E104K/E105K)

    Plasmid

    #171130

    Purpose

    expresses AMOT(E104K/E105K) in mammalian cells

    Depositor

    Wesley Sundquist

    Article

    Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10

    Insert

    Angiomotin p130 E104K/E105K (AMOT Human)
    

    Tags

    2xHA

    Expression

    Mammalian

    Mutation

    changed glutamic acid residues 104 and 105 to lys…

    Available Since

    July 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. KLHL31 pgk (1088)

    Plasmid

    #62031

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

    Insert

    KLHL31 (Klhl31 Mouse)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pCDH-EF1-E-'No'Mi-Red-T2A-Puro

    Plasmid

    #246642

    Purpose

    EF-1alpha driven EV reporter E-'No'Mi-R, a re-engineered tetraspanin scaffold tagged with bioluminescent and fluorescent reporter proteins. Nanoluc and Flag tag are removable with TEVp.

    Depositor

    Koen Breyne

    Article

    Obuchi et al J Extracell Vesicles. 2025 Jun;14(6):e70

    Insert

    cleavable E-NoMi-Red
    

    Use

    Lentiviral

    Tags

    FLAG-Nanoluciferase and mCherry

    Expression

    Mammalian

    Promoter

    EF-1alpha

    Available Since

    Oct. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pENTR-NLS-actin-R62D

    Plasmid

    #118381

    Purpose

    Gateway compatible donor vector with nuclear localization signal (NLS) attached to actin-R62D.

    Depositor

    Maria Vartiainen

    Article

    Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

    Insert

    actin beta (ACTB Human)
    

    Use

    Donor-plasmid

    Mutation

    NLS and R62D

    Available Since

    Nov. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. SuExp His-Myc-hPLD3

    Plasmid

    #173851

    Purpose

    Protein expression plasmid for recombinant His-Myc tag human PLD3

    Depositor

    David Nemazee

    Article

    Gavin et al Nat Commun. 2021 Oct 7;12(1):5874. doi:

    Insert

    PLD3 (PLD3 Human)
    

    Tags

    6xHis, Myc, Factor X cleavable

    Expression

    Mammalian

    Mutation

    intracellular and transmembrane domain truncated,…

    Promoter

    CMV

    Available Since

    Jan. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. SuExp His-Myc-hPLD4

    Plasmid

    #173852

    Purpose

    Protein expression plasmid for recombinant His-Myc tag human PLD4

    Depositor

    David Nemazee

    Article

    Gavin et al Nat Commun. 2021 Oct 7;12(1):5874. doi:

    Insert

    PLD4 (PLD4 Human)
    

    Tags

    6xHis, Myc, Factor X cleavable

    Expression

    Mammalian

    Mutation

    intracellular and transmembrane domain truncated,…

    Promoter

    CMV

    Available Since

    Jan. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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