We narrowed to 5,311 results for: cycs

1. pcDNA3-HA-DNM2-W525L

   Plasmid

   #155263

   Purpose

   Mammalian expression of rat DNM2 fused to HA

   Depositor

   David Rubinsztein

   Article

   Puri et al Dev Cell. 2020 Apr 20;53(2):154-168.e6.

   Insert

   dynamin 2 (Dnm2 Rat)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Tryptophan 525 to Leucine

   Promoter

   Available since

   June 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX330-NQL007-NANOG-sgRNA

   Plasmid

   #175555

   Purpose

   For transient expression of spCas9-nuclease and a sgRNA targeting the mouse NANOG locus.

   Depositor

   Elzo de Wit

   Article

   Liu et al Nat Genet. 2021 Jan;53(1):100-109. doi:

   Insert

   spCas9-nuclease and sgRNA against mouse NANOG STOP Codon
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA5 FRT TO myc Separase S1226A/ C2029A

   Plasmid

   #59823

   Purpose

   Allows the integration of myc Separase S1226A/ C2029A in the genome and Tet-inducible expression.

   Depositor

   Stephen Taylor

   Article

   Holland et al J Cell Sci. 2006 Aug 15;119(Pt 16):3325-

   Insert

   Separase (ESPL1 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   S1226A/ C2029A

   Promoter

   hybrid human cytomegalovirus (CMV)/TetO2 promoter

   Available since

   May 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. sgp27#1

   Plasmid

   #102759

   Purpose

   Expresses sgRNA targeting mouse p27

   Depositor

   Jin Chen

   Article

   Song et al Oncogene. 2017 Jun 5. doi: 10.1038/onc.2

   Insert

   p27 (Cdkn1b Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCGN-HCF-1-N1011Δ382-450

   Plasmid

   #92322

   Purpose

   N-term of HCF-1 protein without amino acids 382-450

   Depositor

   Winship Herr

   Article

   Wilson et al Mol Cell Biol. 1997 Oct;17(10):6139-46.

   Insert

   HCF-1 (HCFC1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   AA 2-1011 with internal deletion Δ382-450

   Promoter

   CMV

   Available since

   Sept. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCGN-HCF-1-N450

   Plasmid

   #92321

   Purpose

   N-term 1-450 of HCF-1 protein

   Depositor

   Winship Herr

   Article

   Wilson et al Mol Cell Biol. 1997 Oct;17(10):6139-46.

   Insert

   HCF-1 (HCFC1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   AA 2-450. Note that a SRRASGTYGRV amino-terminal …

   Promoter

   CMV

   Available since

   Sept. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. px458_2A_GFP_sgRNA_ELAVL2

   Plasmid

   #106107

   Purpose

   CRISPR knockout. Expresses Cas9, EGFP, and sgRNA targeting ELAVL2

   Depositor

   Thomas Tuschl

   Article

   Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

   Insert

   gRNA targeting ELAVL2 (ELAVL2 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA6-E2F4CA-Myc

   Plasmid

   #80428

   Purpose

   Mammalian expression of constitutively active chick E2F4

   Depositor

   José María Frade

   Article

   Morillo et al Mol Cell Biol. 2012 Jul;32(14):2722-37.

   Insert

   E2F transcription factor 4 (E2F4 Chicken)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   T261E, T263E

   Promoter

   CMV

   Available since

   July 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV HA hRb delta CDK + S230

   Plasmid

   #58907

   Purpose

   mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and S230 restored

   Depositor

   Steven Dowdy

   Article

   Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

   Insert

   Rb (RB1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   delta CDK* with S230 restored

   Promoter

   CMV

   Available since

   Oct. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pCMV HA hRB delta CDK + T252

    Plasmid

    #58910

    Purpose

    mammalian expression of human Rb with all Ser/Thr Cdk acceptor sites removed and T252 restored

    Depositor

    Steven Dowdy

    Article

    Narasimha et al Elife. 2014 May 29:e02872. doi: 10.7554/

    Insert

    Rb (RB1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    delta CDK* with T252 restored

    Promoter

    CMV

    Available since

    Oct. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

11. pCAG-p21_T145A

    Plasmid

    #40203

    Depositor

    Philipp Mergenthaler

    Article

    Mergenthaler et al J Cereb Blood Flow Metab. 2013 Mar;33(3)

    Insert

    p21 (CDKN1A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    T145A

    Promoter

    CAG

    Available since

    March 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-p21_T145D

    Plasmid

    #40204

    Depositor

    Philipp Mergenthaler

    Article

    Mergenthaler et al J Cereb Blood Flow Metab. 2013 Mar;33(3)

    Insert

    p21 (CDKN1A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    T145D

    Promoter

    CAG

    Available since

    March 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLV-tetO-Tpbpa

    Plasmid

    #70698

    Purpose

    Lentiviral plasmid for tetracycline/doxycycline dependent expression of murine Tpbpa

    Depositor

    Hubert Schorle

    Article

    Kubaczka et al Cell Stem Cell. 2015 Sep 22. pii: S1934-

    Insert

    Trophoblast specific protein alpha (Tpbpa Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    tetO

    Available since

    Nov. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pSFG-TFII-I-GFP Y248&249F

    Plasmid

    #22196

    Depositor

    Ananda Roy

    Article

    Desgranges et al Mol Cell Biol. 2005 Dec . 25(24):10940-5

    Insert

    TFII-I (GTF2I Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Changed Tyrosines 248 and 249 to Phenylalanines. …

    Promoter

    Available since

    Nov. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

15. 6xHis-TEV-GEI-17(133-509)

    Plasmid

    #87056

    Purpose

    Expresses 6xHis-tagged gei-17 (isoform f), with a TEV protease cleavage site between the tag and the ORF

    Depositor

    Ronald Hay

    Article

    Pelisch et al Nat Commun. 2014 Dec 5;5:5485. doi: 10.1

    Insert

    GEI-17 isfoomr f (aa 133-509) (gei-17 Nematode)
    

    Use

    Tags

    6xHis-TEV and GAGGGS linker

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pX61

    Plasmid

    #46852

    Purpose

    TOP Hupki-puro2A-Hupki-wt plasmid

    Depositor

    Monica Hollstein

    Article

    Wei et al Cell Cycle. 2011 Apr 15;10(8):1261-70. E

    Insert

    attB-Puro-2A and Hupki p53 wild type (TP53 Mouse, Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 21, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. Alt AlphaPS3

    Plasmid

    #14070

    Depositor

    Richard Hynes

    Article

    Stark et al Development. 1997 Nov . 124(22):4583-94.

    Insert

    Drosphila Alt Alpha PS3 Integrin (scb Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

18. pcDNA4c/His-Xpress-HA-hBIG2(WT)

    Plasmid

    #79432

    Purpose

    Expresses N-terminally His6, Xpress and HA-tagged BIG2(WT) in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Shin et al Mol Biol Cell. 2004 Dec;15(12):5283-94.

    Insert

    BIG2 (ARFGEF2 Human)
    

    Use

    Tags

    HA, His6, and Xpress

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA4c/His-Xpress-HA-hBIG1(WT)

    Plasmid

    #79431

    Purpose

    Expresses N-terminally His6, Xpress and HA-tagged BIG1(WT) in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Shin et al Mol Biol Cell. 2004 Dec;15(12):5283-94.

    Insert

    BIG1 (ARFGEF1 Human)
    

    Use

    Tags

    HA, His6, and Xpress

    Expression

    Mammalian

    Mutation

    C1758R

    Promoter

    Available since

    June 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMT1461

    Plasmid

    #228319

    Purpose

    Doxycycline-regulatable ALX-0081 expression

    Depositor

    Jun Ishii

    Article

    Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

    Insert

    MFalpha(2xAdv)-ALX-0081-3A-FLAG-His
    

    Use

    Affinity Reagent/ Antibody

    Tags

    AAA linker followed by FLAG-His6 and MF_ secretio…

    Expression

    Yeast

    Mutation

    Promoter

    Doxycycline-regulatable synthetic promoter (KpTc7…

    Available since

    March 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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