We narrowed to 5,960 results for: actin
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Plasmid#131141PurposeContains ~1kb homology regions flanking Drosophila crk locus. Used to make crk null mutant. Homology regions cloned into AarI and SapI sites of pHD-dsRed-attP (Harrison, O'Connor-Giles, Wildonger).DepositorUseCRISPR and Cre/Lox; Homology directed repair temp…Available SinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only
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pENTR CMVie-Lifeact-EGFP L1-R5
Plasmid#84391PurposeMultisite Gateway entry vector for expression of EGFP-tagged Lifeact in cells including neuronsDepositorInsertLifeact
UseGateway CloningTagsEGFPExpressionMammalianPromoterCMV immediate earlyAvailable SinceJan. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPN10-gDM1d
Plasmid#114387PurposepPN10 with gRNA for spCRISPR-Cas9 against the 3' UTR of the DMPK gene target: TGCGAACCAACGATAGGTG PAM: GGGDepositorInsertgDM1d
UseCRISPRAvailabilityAcademic Institutions and Nonprofits only -
pTRIP TRE BI_SEPT2-14-GFP11_SEPT2-14-GFP10
Plasmid#180350Purposemammalian co-expression of human SEPT2 fused to GFP10 and of human SEPT2 fused to GFP11DepositorUseLentiviralTagsGFP10 and GFP11ExpressionMammalianPromoterbidirectional TREAvailable SinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTRIP TRE BI_GFP11-14-SEPT7_SEPT9_i1-14-GFP10
Plasmid#180348Purposemammalian co-expression of human SEPT9_i1 fused to GFP10 and of human SEPT7 fused to GFP11DepositorUseLentiviralTagsGFP10 and GFP11ExpressionMammalianPromoterbidirectional TREAvailable SinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pnEA-vH_His-TEV-SEPT2-sfCherry2_SEPT6
Plasmid#180313Purposebacterial co-expression of human SEPT2 fused to superfolder Cherry2 and of human SEPT6DepositorTagsHis6-TEV and sfCherry2ExpressionBacterialAvailable SinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
CAG-MCSrev-WPRE-SV40 - GCH4
Plasmid#230832PurposeAAV cloning vector for expression under the CAG promoter.DepositorTypeEmpty backboneUseAAV; Cloning vectorAvailable SinceJan. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
PB CAG-F-bpA-F-L-td-sfGFP
Plasmid#133580PurposeCAG tdGFP FLP/FRT reporter piggyBac transposon (bpA stop)DepositorInsertTandem dimer superfolder GFP
UseFlp/frtExpressionMammalianAvailable SinceMarch 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_Lifeact-HaloTag11-T2A-Tomm20-HaloTag7
Plasmid#175526PurposeCo-expression of HaloTag11 (actin) and 7 (mitochondrial surface) in mammalian cellsDepositorInsertLifeact-HaloTag11 and Tomm20-HaloTag7
ExpressionMammalianMutationHaloTag11 = HaloTag7-M175WAvailable SinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-LifeAct-Venus
Plasmid#87613PurposeActin-targeted Venus (YFP).DepositorInsertLifeAct-Venus
Tags17-aa sequence from Abp140 (aka "Lifeact&quo…ExpressionMammalianPromoterCMVAvailable SinceMarch 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
PX459-CTNNA1(α-catenin CRISPR KO)
Plasmid#229708PurposeKnockout of a-catenin in mammalian cells using CRISPR-Cas9 technologyDepositorAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV(gRNA)-CMV-eGFP-U6(sgCTG)
Plasmid#216732PurposeContains a eGFP gene along with a U6 promoter driving the sgCTG (target sequence: (CUG)6). Used with the HD iPSC-derived astrocytesDepositorArticleInsertsgCTG lentiviral guide RNA with eGFP tag
UseCRISPR and LentiviralExpressionMammalianPromoterCMV and U6Available SinceApril 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV_SEPT9_i1-14-GFP10
Plasmid#180343Purposemammalian expression of human SEPT9_i1 fused to GFP10DepositorAvailable SinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
USPQ-HTT-TALEN1
Plasmid#92243PurposeTALEN targeting upstream of HTT gene (KKR), forms obligate heterodimer with USPQ-HTT-TALEN2DepositorInsertTALEN
UseTALENPromoterCMVAvailable SinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
USPQ-HTT-TALEN2
Plasmid#92244PurposeTALEN targeting upstream of HTT gene (ELD), forms obligate heterodimer with USPQ-HTT-TALEN1DepositorInsertTALEN
UseTALENPromoterCMVAvailable SinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPB-CAG-3xFLAG-empty-pgk-hph
Plasmid#48754PurposePiggyBac backbone, CAG promoter, Hygromycin selection, N-terminal 3xFLAG tagDepositorTypeEmpty backboneUsePiggybacExpressionMammalianPromoterCAGAvailable SinceJuly 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
AAV-CAG-FLEX-LifeAct-jGCaMP8f-WPRE
Plasmid#186047PurposeAAV-mediated expression of LifeAct-jGCaMP8f under the CAG promoter, Cre-dependent expressionDepositorInsertLifeAct-jGCaMP8f
UseAAVTagsLifeAct-6xHisExpressionMammalianPromoterCAGAvailable SinceAug. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV-CAG-FLEX-LifeAct-jGCaMP8m-WPRE
Plasmid#186048PurposeAAV-mediated expression of LifeAct-jGCaMP8m under the CAG promoter, Cre-dependent expressionDepositorInsertLifeAct-jGCaMP8m
UseAAVTagsLifeAct-6xHisExpressionMammalianPromoterCAGAvailable SinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
PB CAG-R-bpA-2xSV40pA-R-td-sfGFP
Plasmid#133578PurposeCAG tdGFP dre/rox reporter piggyBac transposon (bpA 2x SV40pA stop)DepositorInsertTandem dimer superfolder GFP
UseDre/roxExpressionMammalianAvailable SinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRRL-VinculinTS-I997A
Plasmid#111832PurposeVinculin tension sensor with point mutation affecting ability to bind actin in lentiviral expression vectorDepositorInsertVinculinTS (I997A) (VCL Chicken)
UseLentiviralMutationchanged vinculin isoleucine 997 to alaninePromoterCMVAvailable SinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only