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pU6-(BbsI)_CBh-Cas9-T2A-BFP-P2A-Ad4E1B

Plasmid

#64218

Purpose

Expression vector for sgRNA and for Expression of Cas9 linked to BFP via T2A linked to Ad4 E1B via P2A

Depositor

Ralf Kuehn

Article

Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

Inserts

Cas9
sgRNA cassette

Use

CRISPR

Tags

3xFLAG, NLS, and T2A-BFP-P2A-E1B

Expression

Mammalian

Promoter

CBh and U6

Available Since

May 19, 2015

Availability

Academic Institutions and Nonprofits only

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mTFP1-N1

Plasmid

#54521

Purpose

Localization: N1 Cloning Vector, Excitation: 462, Emission: 492

Depositor

Robert Campbell, Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Type

Empty backbone

Tags

mTFP1

Expression

Mammalian

Available Since

June 20, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3 HA eIF4GI (1-1599)

Plasmid

#45640

Depositor

Nahum Sonenberg

Article

Yanagiya et al Mol Cell Biol. 2009 Mar;29(6):1661-9. do

Insert

eIF4GI transcript variant 2 (EIF4G1 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

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AmCyan-P2A-mCherry

Plasmid

#45350

Depositor

Ilpo Huhtaniemi

Article

Potorac et al J Endocrinol. 2016 Sep 21. pii: JOE-16-0

Insert

AmCyan

Tags

mCherry

Expression

Mammalian

Mutation

SV40 NLS

Promoter

CMV

Available Since

Nov. 6, 2013

Availability

Academic Institutions and Nonprofits only

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LeGO-iG2

Plasmid

#27341

Depositor

Boris Fehse

Article

Weber et al Mol Ther. 2008 Apr . 16(4):698-706.

Insert

SFFV promoter, IRES-eGFP

Use

Lentiviral

Expression

Mammalian

Available Since

April 24, 2012

Availability

Academic Institutions and Nonprofits only

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myc-hULK1

Plasmid

#31961

Depositor

Do-Hyung Kim

Article

Jung et al Mol Biol Cell. 2009 Apr;20(7):1992-2003.

Insert

ULK1 (ULK1 Human)

Tags

myc

Expression

Mammalian

Promoter

CMV, SP6

Available Since

Sept. 1, 2011

Availability

Academic Institutions and Nonprofits only

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Lenti-sh1368 knockdown c-myc

Plasmid

#29435

Depositor

Bob Eisenman

Article

Lin et al EMBO J. 2009 Oct 21. 28(20):3157-70.

Insert

c-Myc shRNA (MYC Human)

Use

Lentiviral and RNAi

Tags

GFP and WPRE

Expression

Mammalian

Available Since

May 6, 2011

Availability

Academic Institutions and Nonprofits only

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3XFlag pCMV5-TOPO TAZ WT

Plasmid

#24809

Depositor

Jeff Wrana

Article

Varelas et al Nat Cell Biol. 2008 Jul . 10(7):837-48.

Insert

TAZ (WWTR1 Human)

Tags

3XFlag

Expression

Mammalian

Available Since

May 14, 2010

Availability

Industry, Academic Institutions, and Nonprofits

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pLenti CMV RasV12 Neo (w108-1)

Plasmid

#22259

Depositor

Eric Campeau

Insert

HRAS G12V (HRAS Human)

Use

Lentiviral

Expression

Mammalian

Mutation

G12V mutant

Available Since

Jan. 4, 2010

Availability

Academic Institutions and Nonprofits only

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pAc-Neo-OVA

Plasmid

#22533

Depositor

Michael Bevan

Article

Moore et al Cell. 1988 Sep 9. 54(6):777-85.

Insert

OVA (OVAL Chicken)

Expression

Mammalian

Available Since

Dec. 28, 2009

Availability

Academic Institutions and Nonprofits only

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pAL175

Plasmid

#22276

Purpose

Expresses YFP-PIF for red-light-induced binding to Phy

Depositor

Christopher Voigt

Article

Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

Insert

PIF6APB (PIL2 Mustard Weed)

Use

Synthetic Biology

Tags

mYFP

Expression

Mammalian

Promoter

SV40

Available Since

Oct. 21, 2009

Availability

Academic Institutions and Nonprofits only

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pGL2Basic-EcadK1

Plasmid

#19290

Depositor

Eric Fearon

Article

Hajra et al Oncogene. 1999 Dec 2. 18(51):7274-9.

Insert

E-cadherin

Use

Luciferase

Expression

Mammalian

Mutation

E-cad promoter sequences from -108 to +125

Available Since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only

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MSCV PIG (Puro IRES GFP)

Plasmid

#18751

Depositor

Scott Lowe

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Available Since

July 3, 2008

Availability

Academic Institutions and Nonprofits only

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pMIG-hOCT4

Plasmid

#17225

Depositor

George Daley

Article

Park et al Nature. 2008 Jan 10. 451(7175):141-6.

Insert

POU class 5 homeobox 1 (POU5F1 Human)

Use

Retroviral

Expression

Mammalian

Available Since

Jan. 18, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA3-DN-hCUL4A-FLAG

Plasmid

#15821

Depositor

Wade Harper

Article

Jin et al Methods Enzymol. 2005;399:287-309. doi:

Insert

Cullin4A (CUL4A Human)

Tags

Flag

Expression

Mammalian

Mutation

Dominant negative: N440

Available Since

Sept. 28, 2007

Availability

Academic Institutions and Nonprofits only

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HA-CYLD-WT

Plasmid

#15506

Depositor

Stephen Elledge

Article

Stegmeier et al Proc Natl Acad Sci U S A. 2007 May 22. 1

Insert

CYLD (CYLD Human)

Tags

HA

Expression

Mammalian

Available Since

Aug. 13, 2007

Availability

Academic Institutions and Nonprofits only

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tFucci(SA)5

Plasmid

#153520

Purpose

New color variant of Fucci (Fluorescent ubiquitination-based cell cycle indicator) : Fucci(SA)5.

Depositor

Atsushi Miyawaki

Article

Ando et al Cell Struct Funct. 2023 Jun 30. doi: 10.

Insert

tFucci(SA)5.

Tags

AzaleaB5-hCdt1(30/120)_P2A_h2-3-hGem(1/110)

Expression

Bacterial and Mammalian

Promoter

CAG

Available Since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pLX304 CMV HA-HaloTag-FRB-sTurboID-high affinity (C)

Plasmid

#153005

Purpose

Expresses high-affinity split-TurboID C-terminal fragment in mammalian cells

Depositor

Alice Ting

Article

Cho et al Proc Natl Acad Sci U S A. 2020 May 18. p

Insert

sTurboID-high affinity (C) (G79)

Use

Lentiviral

Tags

FRB, HA, and HaloTag

Expression

Mammalian

Promoter

CMV

Available Since

June 16, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-Blast-PIP-FUCCI

Plasmid

#138715

Purpose

Lentiviral expression of the cell cycle marker PIP-FUCCI

Depositor

Jean Cook

Article

Grant et al Cell Cycle. 2018;17(21-22):2496-2516. do

Insert

PIP-FUCCI

Use

Lentiviral

Tags

mCherry and mVenus

Expression

Mammalian

Promoter

CMV

Available Since

March 19, 2020

Availability

Academic Institutions and Nonprofits only

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pSG5-HA-p300

Plasmid

#89094

Purpose

mammalian expression of full-length human p300 in pSG5

Depositor

Elizabeth Wilson

Article

Askew et al J Biol Chem. 2010 Jul 9;285(28):21824-36

Insert

p300 (EP300 Human)

Tags

HA

Expression

Mammalian

Promoter

SV40

Available Since

April 21, 2017

Availability

Academic Institutions and Nonprofits only

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