8,812 results

1. ERM-APEX2

   Plasmid

   #79055

   Purpose

   Soybean APEX2 anchored to cytosolic face of ER membrane with N-terminal targeting sequence of residues 1-27 of ER-resident P450 oxidase 2C1.

   Depositor

   Alice Ting

   Article

   Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n

   Insert

   APEX2 anchored to the cytosolic face of the ER membrane
   

   Use

   Lentiviral

   Tags

   Residues 1-27 of P450 oxidase 2C1 and V5

   Expression

   Bacterial and Mammalian

   Promoter

   CMV immearly promotor

   Available Since

   Aug. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. EF.CMV.RFP

   Plasmid

   #17619

   Depositor

   Linzhao Cheng

   Article

   Yu et al Mol Ther. 2003 Jun . 7(6):827-38.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   March 27, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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3. Cas9-T6N (pZZ12, TAT-4MycNLS-Cas9-2SV40NLS-sfGFP)

   Plasmid

   #199604

   Purpose

   Expression of cell permeable spCas9 in E. Coli.

   Depositor

   Junwei Shi

   Article

   Zhang et al Nat Biotechnol. 2023 Apr 24. doi: 10.103

   Insert

   Cas9-T6N
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   April 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. NFAT luciferase reporter

   Plasmid

   #10959

   Depositor

   Toren Finkel

   Article

   Ichida et al J Biol Chem. 2001 Feb 2. 276(5):3524-30.

   Insert

   IL-2 promoter (IL2 Human)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Available Since

   Jan. 6, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. MSCV_Cas9_puro

   Plasmid

   #65655

   Purpose

   Retroviral introduction of Cas9 into mammalian cell line.

   Depositor

   Christopher Vakoc

   Article

   Shi et al Nat Biotechnol. 2015 May 11. doi: 10.103

   Insert

   Cas9
   

   Use

   CRISPR and Retroviral

   Tags

   5 ' FLAG tag

   Expression

   Mammalian

   Promoter

   MSSV_LTR

   Available Since

   June 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. MSCV Luciferase PGK-hygro

   Plasmid

   #18782

   Depositor

   Scott Lowe

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Luciferase and Retroviral

   Expression

   Mammalian

   Available Since

   July 3, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAdTrack

   Plasmid

   #16404

   Purpose

   Shuttle vector for us in AdEasy System. Used for expression of transgenes under a chosen (non-CMV) promoter when a GFP tracer is desired.

   Depositor

   Bert Vogelstein

   Article

   He et al Proc Natl Acad Sci U S A. 1998 Mar 3. 95

   Type

   Empty backbone

   Use

   Adenoviral

   Expression

   Mammalian

   Available Since

   June 11, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRL443

   Plasmid

   #70261

   Purpose

   Shuttle vector suitable for mobilizing plasmids to Anabaena (Nostoc) sp. strain PCC 7120 and other cyanobacterial strains.

   Depositor

   Peter Wolk

   Article

   Elhai et al J Bacteriol. 1997 Mar;179(6):1998-2005.

   Type

   Empty backbone

   Available Since

   Feb. 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. Pcr4-12xMBS-PBS

   Plasmid

   #52984

   Purpose

   provide MBS-PBS alternating sequence

   Depositor

   Robert Singer

   Article

   Wu et al Sci Rep. 2014 Jan 9;4:3615. doi: 10.1038

   Insert

   12xMBS-PBS
   

   Expression

   Bacterial

   Promoter

   none

   Available Since

   June 23, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. CMV-hRad51

    Plasmid

    #125570

    Purpose

    Expresses the Rad51 protein in mammalian cells under CMV promoter

    Depositor

    David Liu

    Article

    Rees et al Nat Commun. 2019 May 17;10(1):2212. doi:

    Insert

    Rad51 (RAD51 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    May 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFC332

    Plasmid

    #87845

    Purpose

    AMA1 plasmid with Aspergillus optimized Cas9 and hph selection marker

    Depositor

    Uffe Mortensen

    Article

    Nodvig et al PLoS One. 2015 Jul 15;10(7):e0133085. do

    Inserts

    Cas9
    hph (hygromycin resistance marker)
    

    Use

    CRISPR; Fungal expression

    Tags

    SV40 NLS

    Expression

    Bacterial

    Promoter

    Aspergillus nidulans tef1 promoter and Aspergillu…

    Available Since

    May 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-PEmaxRNaseHtrunc

    Plasmid

    #207858

    Purpose

    Mammalian expression of PEmaxDRNaseH prime editor

    Depositor

    David Liu

    Article

    Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

    Insert

    PEmaxDRNaseH
    

    Tags

    SV40 bpNLS and SV40 bpNLS, c-Myc NLS

    Expression

    Mammalian

    Mutation

    M-MLVRTDRNAseHrt

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. SpyTag003-MBP

    Plasmid

    #133450

    Purpose

    Expresses SpyTag003-MBP (maltose binding protein) in bacterial cytoplasm

    Depositor

    Mark Howarth

    Article

    Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

    Insert

    SpyTag003-MBP
    

    Tags

    His6 and Thrombin cleavage site

    Expression

    Bacterial

    Mutation

    Peptide tag added to N-terminus of MBP via a GSGE…

    Promoter

    T7

    Available Since

    Dec. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. mEos3.2-C1

    Plasmid

    #54550

    Purpose

    Localization: C1 Cloning Vector, Excitation: 507 / 572, Emission: 516 / 580

    Depositor

    Michael Davidson, Tao Xu

    Article

    Zhang et al Nat Methods. 2012 May 13;9(7):727-9. doi

    Type

    Empty backbone

    Tags

    mEos3.2

    Expression

    Mammalian

    Available Since

    June 12, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pRT029

    Plasmid

    #127969

    Purpose

    double-DHFR-degron controlled expression of dCas9-BFP-KRAB from the CLYBL locus

    Depositor

    Martin Kampmann

    Article

    Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3

    Insert

    CLYBL-CAG-DHFR-dCas9-BFP-KRAB-DHFR
    

    Use

    TALEN

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Aug. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDusk

    Plasmid

    #43795

    Purpose

    Empty backbone for blue-light-regulated expression of a gene of interest. Expression is repressed under blue illumination.

    Depositor

    Andreas Moeglich

    Article

    Ohlendorf et al J Mol Biol. 2012 Mar 2;416(4):534-42. do

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    April 9, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. CMV-Hylight

    Plasmid

    #193447

    Purpose

    A fluorescent biosensor for fructose 1,6-bisphosphate (FBP). Changes in FBP concentration strongly correlate with changes in glycolytic flux. (mammalian expression vector)

    Depositor

    Richard Goodman

    Article

    Koberstein et al Proc Natl Acad Sci U S A. 2022 Aug 2;119

    Insert

    Hylight Biosensor
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCytochrome C-GFP

    Plasmid

    #41182

    Depositor

    Douglas Green

    Article

    Goldstein et al Nat Cell Biol. 2000 Mar;2(3):156-62.

    Insert

    Cytochrome C
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pT3-EF1a-c-Met

    Plasmid

    #31784

    Depositor

    Xin Chen

    Article

    Tward et al Proc Natl Acad Sci U S A. 2007 Sep 11;10

    Insert

    Met (MET Human)
    

    Expression

    Mammalian

    Promoter

    EF1alpha

    Available Since

    Oct. 21, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. shCD44-2 pRRL

    Plasmid

    #19123

    Depositor

    Bob Weinberg

    Article

    Godar et al Cell. 2008 Jul 11. 134(1):62-73.

    Insert

    small hairpin RNA against CD44 (CD44 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Oct. 2, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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