We narrowed to 7,632 results for: ALP

Showing: 5701 - 5750 of 7632 results

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pCS4

Plasmid

#55751

Purpose

Produces one half (alpha) of aminoglycoside phosphotransferase (3′)-IIa when in the presence of T7 RNA Polymerase.

Depositor

Matthew Bennett

Article

Schmidt et al ACS Synth Biol. 2012 Oct 19;1(10):445-50

Insert

KanR

Expression

Bacterial

Mutation

Residues 1-59 of KanR

Promoter

T7-lac

Available Since

July 15, 2014

Availability

Academic Institutions and Nonprofits only

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pMA2867

Plasmid

#25439

Depositor

Mikhail Alexeyev

Article

Alexeyev et al Mol Biol Rep. 2010 Apr . 37(4):1987-91.

Insert

mCherry-miR155-lacZalpha (Mir155hg Mouse, Discosoma-mouse-E. coli)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Sept. 13, 2010

Availability

Academic Institutions and Nonprofits only

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pWKS1583

Plasmid

#70188

Purpose

Modular vector for secreted amylase reporter expression

Depositor

Wiep Klaas Smits

Article

Oliveira Paiva et al ACS Synth Biol. 2016 Jun 23.

Insert

amyEopt

Use

Synthetic Biology

Expression

Bacterial

Promoter

Ptet

Available Since

June 27, 2016

Availability

Academic Institutions and Nonprofits only

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pNLRP11major.MYC

Plasmid

#131196

Purpose

Mammalian expression of human NLRP11 188S

Depositor

Thomas Kufer

Article

Ellwanger et al J Biol Chem. 2018 Jan 4. pii: RA117.0001

Insert

NLRP11 188S (NLRP11 Human)

Tags

myc

Expression

Mammalian

Mutation

188S

Promoter

pCMV

Available Since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

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FRB-polySH33-EGFP

Plasmid

#175792

Purpose

Bacterial expression and purification, fluorescently tagged scaffold for droplet visualization and volume quantification

Depositor

Michael Rosen

Article

Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

Insert

NCK1 (NCK1 Human)

Tags

His6 and MBP

Expression

Bacterial

Promoter

tac

Available Since

Nov. 3, 2021

Availability

Academic Institutions and Nonprofits only

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SGL40C-H1.EFS.RFP657

Plasmid

#69148

Purpose

Advanced lentiviral Vector for sgRNA (H1 Promoter) delivery with RFP657 expression (EFS Promoter)

Depositor

Dirk Heckl

Article

Reimer et al Haematologica. 2017 Jun 1. pii: haematol

Inserts

sgRNA
EFS
tagRFP657

Available Since

June 5, 2017

Availability

Academic Institutions and Nonprofits only

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OMM PKA Reporter

Plasmid

#104550

Purpose

Expresses Tom20-mTurquoise2-VASP at the outer mitochondrial membrane of mammalian cells.

Depositor

David Lawrence

Article

O'Banion et al Cell Chem Biol. 2017 Oct 25. pii: S2451-

Insert

Tom20 - mTurquoise2 - VASP 148-164

Tags

mTurqouise2

Expression

Mammalian

Promoter

EF1 alpha

Available Since

March 6, 2018

Availability

Academic Institutions and Nonprofits only

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pME-puro-CLIP-FLAG-CD59

Plasmid

#50375

Purpose

Express CLIP and FLAG-tagged human CD59, a GPI-AP in mammmalian cells

Depositor

Reika Watanabe

Article

Castillon et al Mol Biol Cell. 2013 Jun;24(12):2021-33.

Insert

CLIP

Expression

Mammalian

Promoter

SR alpha

Available Since

July 15, 2014

Availability

Academic Institutions and Nonprofits only

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pAS1_4x7SKPylT_EF1_Ub-BsmBI_IRES_BSD

Plasmid

#162804

Purpose

STELLA destination plasmid containing restriction sites for golden gate cloning. Suitable for amber suppression (4xMmaPylT cassette); for transient or stable piggyBac-mediated integration

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

Ub-BsmBI_IRES_Blasticidin

Use

Piggybac

Expression

Mammalian

Promoter

EF1alpha

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pEBG-hBEN

Plasmid

#22160

Depositor

Ananda Roy

Article

Tussie-Luna et al Proc Natl Acad Sci U S A. 2001 Jul 3. 98

Insert

hBEN (GTF2IRD1 Human)

Tags

GST and His

Expression

Mammalian

Available Since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only

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mPapaya1-PDHA1-N-10

Plasmid

#56356

Purpose

Localization: Mitochondria, Excitation: 530, Emission: 541

Depositor

Michael Davidson

Article

Hoi et al Chem Biol. 2013 Oct 24;20(10):1296-304.

Insert

PDHA1 (PDHA1 Human)

Tags

mPapaya1

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pCaSper4 cyto roGFP2-Orp1

Plasmid

#64998

Purpose

Expression of cytosolic roGFP2-Orp1 under the alphaTub84B promoter

Depositor

Tobias Dick

Article

Albrecht et al Cell Metab. 2011 Dec 7;14(6):819-29. doi

Insert

Orp1 (HYR1 Budding Yeast)

Tags

roGFP2

Expression

Insect

Available Since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

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GFP-mROCK1-delta4

Plasmid

#101292

Purpose

Mammalian expression of Rock1-GFP for the study of localisation signaling.

Depositor

Alpha Yap

Article

Priya et al Nat Cell Biol. 2015 Oct;17(10):1282-93.

Insert

Rock1 (Rock1 Mouse)

Expression

Mammalian

Mutation

76-1354 aa

Promoter

CMV

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pEBB-GFP-hBEN

Plasmid

#22157

Depositor

Ananda Roy

Article

Tussie-Luna et al Proc Natl Acad Sci U S A. 2001 Jul 3. 98

Insert

hBEN (GTF2IRD1 Human)

Tags

GFP

Expression

Mammalian

Available Since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only

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pMpGWB310

Plasmid

#68638

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

3xFLAG

Expression

Plant

Promoter

MpEF1alpha promoter

Available Since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

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TRF2-HA

Plasmid

#200209

Purpose

Expression of full length mTRF2

Depositor

Sheila Teves

Article

Kwan et al Elife. 2023 Mar 30;12:e83810. doi: 10.75

Insert

TRF2-HA (Terf2 Mouse)

Tags

HA

Expression

Mammalian

Mutation

None

Promoter

EF_1alpha

Available Since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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pCEP4_OCT4_T234A_S235A

Plasmid

#40630

Depositor

James Thomson

Article

Brumbaugh et al Proc Natl Acad Sci U S A. 2012 May 8;109

Insert

POU5F1_S234A_T235A (POU5F1 Human)

Expression

Mammalian

Mutation

S234A_T235A

Promoter

EF1-alpha

Available Since

July 26, 2013

Availability

Academic Institutions and Nonprofits only
GFP-mROCK1-delta6

Plasmid

#101294

Purpose

Mammalian expression of Rock1-GFP for the study of localisation signaling.

Depositor

Alpha Yap

Article

Priya et al Nat Cell Biol. 2015 Oct;17(10):1282-93.

Insert

Rock1 (Rock1 Mouse)

Expression

Mammalian

Mutation

375-1354 aa

Promoter

CMV

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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TRAPa-3xFLAG-pCDNA

Plasmid

#201963

Purpose

Mammalian expression of Translocon-associated protein subunit alpha

Depositor

Ville Paavilainen

Article

Karki et al EMBO Rep. 2023 Dec 6;24(12):e57910. doi:

Insert

SSR1 (SSR1 Human)

Tags

3xFLAG-tag

Expression

Mammalian

Available Since

Jan. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pCEP4_OCT4_T234E_S235E

Plasmid

#40631

Depositor

James Thomson

Article

Brumbaugh et al Proc Natl Acad Sci U S A. 2012 May 8;109

Insert

POU5F1 (POU5F1 Human)

Expression

Mammalian

Mutation

T234E_S235E

Promoter

EF1-alpha

Available Since

July 26, 2013

Availability

Academic Institutions and Nonprofits only
Nup54 no FG-mEGFP494(0)

Plasmid

#164269

Purpose

Mammalian expression of Nup54 without the FG-Nup domain, with mEGFP (missing the first 5 amino acids) at amino acid position 494 with a rigid alpha helix of 5 amino acids at the carboxyl end of mEGFP

Depositor

Sanford Simon

Article

Pulupa et al Elife. 2020 Dec 21;9. pii: 60654. doi: 1

Insert

Nup54 (NUP54 Human)

Tags

mEGFP

Expression

Mammalian

Mutation

deleted the FG region of Nup54

Promoter

CMV

Available Since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221-OSF-TRIM5-21R

Plasmid

#79063

Purpose

Gateway entry vector for cloning and propagation in DH5alpha

Depositor

Wesley Sundquist

Article

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

OSF-TRIM5-21R

Tags

OneSTrEP-FLAG

Expression

Insect

Promoter

T7

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pET11a-CA(A14C, E45C, A92E)

Plasmid

#79046

Purpose

Bacterial vector for cloning and propagation in DH5alpha

Depositor

Wesley Sundquist

Article

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

CA (A14C, E45C, A92E)

Expression

Bacterial

Mutation

A14C, E45C, A92E mutations in HIV-1 CA protein

Promoter

T7

Available Since

March 22, 2017

Availability

Academic Institutions and Nonprofits only

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pBGSA FPR1

Plasmid

#62600

Purpose

Constitutive expression of FPR1 in mammalian cells

Depositor

Heini Miettinen

Article

Miettinen et al J Immunol. 1997 Oct 15;159(8):4045-54.

Insert

FPR1 (FPR1 Human)

Expression

Mammalian

Promoter

SRalpha

Available Since

Feb. 24, 2015

Availability

Academic Institutions and Nonprofits only
pLV-REPORT(EF1a no GFP) mnuctdTomato

Plasmid

#172342

Purpose

mnuctdTomato

Depositor

David Bersten

Article

Allen et al bioRxiv 2023.11.21.568191

Inserts

monomeric nuclear Tomato
HygR

Use

Lentiviral and Synthetic Biology

Tags

SV40 NLS

Expression

Mammalian

Promoter

EF1alpha

Available Since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pIIS18-SapI

Plasmid

#128599

Purpose

Entry vector useful for golden gate or other seamless cloning technique based on Type IIS restriction enzymes

Depositor

Tohru Suzuki

Article

Altaib et al Microbiol Resour Announc. 2019 Oct 10;8(

Type

Empty backbone

Use

Unspecified

Available Since

Feb. 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pIIS18-BsmBI

Plasmid

#128648

Purpose

Entry vector useful for golden gate or other seamless cloning technique based on Type IIS restriction enzymes

Depositor

Tohru Suzuki

Article

Altaib et al Microbiol Resour Announc. 2019 Oct 10;8(

Type

Empty backbone

Use

Unspecified

Available Since

Feb. 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-mROCK1-delta7

Plasmid

#101295

Purpose

Mammalian expression of Rock1-GFP for the study of localisation signaling.

Depositor

Alpha Yap

Article

Priya et al Nat Cell Biol. 2015 Oct;17(10):1282-93.

Insert

Rock1 (Rock1 Mouse)

Expression

Mammalian

Mutation

727-1354 aa

Promoter

CMV

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
F5A deltaCT S6K1 MSCV puro

Plasmid

#8464

Depositor

David Sabatini

Article

Ali et al J Biol Chem 2005 Apr 4;.

Insert

f5a delta ctp70 S6K1 (Rps6kb1 Rat)

Use

Retroviral

Tags

myc

Expression

Mammalian

Mutation

deletion of 104 c-terminal amino acids mutation …

Available Since

April 4, 2006

Availability

Academic Institutions and Nonprofits only
SGL40C.EFS.RFP657

Plasmid

#69147

Purpose

Advanced lentiviral Vector for sgRNA (hU6 Promoter) delivery with RFP657 expression (EFS Promoter)

Depositor

Dirk Heckl

Article

Reimer et al Haematologica. 2017 Jun 1. pii: haematol

Inserts

sgRNA
EFS
tagRFP657

Available Since

Aug. 2, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pMX155

Plasmid

#29481

Depositor

Linda Wordeman

Article

Moore et al J Cell Biol. 2005 May 9. 169(3):391-7.

Insert

Kif2A-alpha (Kif2a Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

E140K

Available Since

April 7, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pMpGWB436

Plasmid

#68701

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

TagRFP

Expression

Plant

Promoter

MpEF1alpha promoter

Available Since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pEF1α-ancestral-retroCHMP3-HA

Plasmid

#154248

Purpose

expresses ancestral New World monkey retroCHMP3 in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al Curr Biol. 2022 Feb 25. pii: S0960-9822(

Insert

reconstructed ancestral sequence of New World monkey retroCHMP3

Tags

HA

Expression

Mammalian

Promoter

EF1-alpha

Available Since

April 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pEF1α-owl-monkey-retroCHMP3-HA

Plasmid

#154249

Purpose

expresses owl monkey retroCHMP3 in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al Curr Biol. 2022 Feb 25. pii: S0960-9822(

Insert

retroCHMP3

Tags

HA

Expression

Mammalian

Promoter

EF1-alpha

Available Since

April 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pDownstream1Lox

Plasmid

#164574

Purpose

Integration of 1 LoxN site in the genome of P. berghei

Depositor

Olivier Silvie

Type

Empty backbone

Use

Cre/Lox

Promoter

Bidirectional PbEF1alpha promoter (PBANKA_1133300…

Available Since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pMpGWB224

Plasmid

#68615

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

Triple repeats of Citrine (3xCitrine)

Expression

Plant

Promoter

MpEF1alpha promoter

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pMpGWB324

Plasmid

#68652

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

Triple repeats of Citrine (3xCitrine)

Expression

Plant

Promoter

MpEF1alpha promoter

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pMpGWB213

Plasmid

#68604

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

Glucocorticoid receptor (GR)

Expression

Plant

Promoter

MpEF1alpha promoter

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
Col7A1-CRISPR-gRNA#exon3-(pSpCas9(BB)-2A-Puro (PX459) V2.0)

Plasmid

#124287

Purpose

sgRNA against human Col7A1

Depositor

Katalin Josvay, Csaba Vizler

Insert

Collagen type VII alpha 1 chain (COL7A1 Human)

Use

CRISPR

Available Since

April 3, 2019

Availability

Academic Institutions and Nonprofits only
pWPI-NP1

Plasmid

#65060

Purpose

Constitutive expression of NP1

Depositor

Ramon Trullas

Article

Figueiro-Silva et al J Neurosci. 2015 Apr 8;35(14):5504-21. d

Insert

Neuronal Pentraxin 1 (Nptx1 Rat)

Use

Lentiviral

Expression

Mammalian

Mutation

* see comments

Promoter

EF1alpha

Available Since

July 29, 2015

Availability

Academic Institutions and Nonprofits only
pPKm-145

Plasmid

#90505

Purpose

Empty plasmid, pSIN-EF1-alpha-IRES-puro

Depositor

Todd Coleman

Article

Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

Type

Empty backbone

Use

Lentiviral

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-mROCK1-delta5

Plasmid

#101293

Purpose

Mammalian expression of Rock1-GFP for the study of localisation signaling.

Depositor

Alpha Yap

Article

Priya et al Nat Cell Biol. 2015 Oct;17(10):1282-93.

Insert

Rock1 (Rock1 Mouse)

Expression

Mammalian

Mutation

375-727 aa

Promoter

CMV

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
acta 2

Plasmid

#78711

Purpose

zebrafish acta2 3-UTR–specific probe

Depositor

Sarah Childs

Article

Georgijevic et al Dev Dyn. 2007 Jun;236(6):1623-32.

Insert

acta2 (acta2 Zebrafish)

Expression

Bacterial

Mutation

contains bp 1,243–1,591

Available Since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only
pACT2 V1H (P#663)

Plasmid

#14665

Depositor

Matija Peterlin

Article

Geyer et al Mol Biol Cell. 2002 Jun . 13(6):2045-56.

Insert

V1H (ATP6V1H Human)

Tags

Gal4 AD

Expression

Yeast

Available Since

June 30, 2008

Availability

Academic Institutions and Nonprofits only
pAS1_4x7SKPylT_EF1_Ub-BsmBI

Plasmid

#162801

Purpose

STELLA destination plasmid containing restriction sites for golden gate cloning. Suitable for amber suppression (4xMmaPylT cassette); for transient expression

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

Ub-BsmBI

Use

Piggybac

Expression

Mammalian

Promoter

EF1alpha

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pDM3193

Plasmid

#113390

Purpose

Alpha factor inducible Ty3 with his3AI

Depositor

Suzanne Sandmeyer

Article

Bilanchone et al PLoS Genet. 2015 Sep 30;11(9):e1005528.

Insert

Ty3-his3AI

Tags

his3AI

Expression

Yeast

Available Since

Aug. 17, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pCellFree_G03 TCF3

Plasmid

#67110

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

TCF3 (TCF3 Human)

Use

Cell-free expression

Tags

EGFP and His

Mutation

Truncated isoform

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pUPD2-ODPAMTS (12)

Plasmid

#101694

Purpose

ODPAMTS in domestication vector

Depositor

Elena Caro Bernat

Article

Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

Insert

CCAT-[MTS from S. cerevisiae Pyruvate dehydrogenase E1 component subunit alpha (YER178W)]-AATG

Expression

Yeast

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
mPapaya-PDHA1-N-10

Plasmid

#56349

Purpose

Localization: Mitochondria, Excitation: 530, Emission: 541

Depositor

Michael Davidson

Article

Hoi et al Chem Biol. 2013 Oct 24;20(10):1296-304.

Insert

PDHA1 (PDHA1 Human)

Tags

mPapaya

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 18, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pNLRP11minor.MYC

Plasmid

#131195

Purpose

Mammalian expression of human NLRP11 188A

Depositor

Thomas Kufer

Article

Ellwanger et al J Biol Chem. 2018 Jan 4. pii: RA117.0001

Insert

NLRP11 188A (NLRP11 Human)

Tags

myc

Expression

Mammalian

Mutation

188A

Promoter

pCMV

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge

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We narrowed to 7,632 results for: ALP