We narrowed to 7,223 results for: ALP

1. pnYC-NvHN-DmBam_13-36_AF

   Plasmid

   #148742

   Purpose

   Bacterial Expression of DmBam_13-36

   Depositor

   Elisa Izaurralde

   Article

   Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

   Insert

   DmBam_13-36 (bam Fly)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. GB1839

   Plasmid

   #193103

   Purpose

   GB-cassette for the expression of guide RNA targeting the DFR promoter in -376 position (gRNA2), with 2.1 Ms2 aptamer in the 3' of the scaffold.

   Depositor

   Diego Orzaez

   Article

   Moreno-Gimenez et al ACS Synth Biol. 2022 Sep 16;11(9):3037-3

   Insert

   GB_SynP gRNA2
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. p663-UBC-miniTurbo-V5-CSNK2A2_IDG-K

   Plasmid

   #189870

   Purpose

   Gateway destination clone of CSNK2A2 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   CSNK2A2 (CSNK2A2 Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   miniTurbo-V5

   Expression

   Mammalian

   Mutation

   Promoter

   Ubiquitin

   Available since

   Oct. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. MAC_N_KLF10

   Plasmid

   #187734

   Purpose

   MAC-tagged gene expression of human KLF10

   Depositor

   Markku Varjosalo

   Article

   Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

   Insert

   KLF10 (KLF10 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCSIIpuro-miRFP670-eDHFR(69K6)

   Plasmid

   #178852

   Purpose

   Mammalian expression of miRFP670-eDHFR(69K6)

   Depositor

   Shinya Tsukiji

   Article

   Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

   Insert

   miRFP670-eDHFR(69K6)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1-alpha

   Available since

   July 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCSIIpuro-mNG-eDHFR(69K6)

   Plasmid

   #178853

   Purpose

   Mammalian expression of mNeonGreen-eDHFR(69K6)

   Depositor

   Shinya Tsukiji

   Article

   Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

   Insert

   mNeonGreen-eDHFR(69K6)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1-alpha

   Available since

   July 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. hCMV-IE1:RoLuc:bgH

   Plasmid

   #135290

   Purpose

   Transcriptional unit for constitutive expression of RoLuc in mammalian cells

   Depositor

   Koen Venken

   Article

   Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

   Insert

   CMV:RoLuc:bGH
   

   Use

   Luciferase and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. hCMV-IE1:MetLuc:bgH

   Plasmid

   #135292

   Purpose

   Transcriptional unit for constitutive expression of MetLuc in mammalian cells

   Depositor

   Koen Venken

   Article

   Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

   Insert

   CMV:MetLuc:bGH
   

   Use

   Luciferase and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pEF1α-woolly-monkey-retroCHMP3(148)-HA

   Plasmid

   #154250

   Purpose

   expresses prematurely truncated woolly monkey retroCHMP3 in mammalian cells

   Depositor

   Wesley Sundquist

   Article

   Rheinemann et al Curr Biol. 2022 Feb 25. pii: S0960-9822(

   Insert

   retroCHMP3
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   C-terminal truncation of retroCHMP3 at amino acid…

   Promoter

   EF1-alpha

   Available since

   April 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pEF1α-pygmy-marmoset-retroCHMP3(148)-HA

    Plasmid

    #154251

    Purpose

    expresses prematurely truncated pygmy marmoset retroCHMP3 in mammalian cells

    Depositor

    Wesley Sundquist

    Article

    Rheinemann et al Curr Biol. 2022 Feb 25. pii: S0960-9822(

    Insert

    retroCHMP3
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    C-terminal truncation of retroCHMP3 at amino acid…

    Promoter

    EF1-alpha

    Available since

    April 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. ST*-GFP

    Plasmid

    #163667

    Purpose

    Expresses partial length ST in mammalian cells

    Depositor

    Lei Lu

    Article

    Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

    Insert

    partial length ST6 beta-galactoside alpha-2,6-sialyltransferase 1 (first 113 amino acids) (ST6GAL1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    This chimera contains the first 113 amino acids o…

    Promoter

    Available since

    Nov. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pEF1α-squirrel-monkey-full-length-CHMP3-HA

    Plasmid

    #154172

    Purpose

    expresses squirrel monkey CHMP3 in mammalian cells

    Depositor

    Wesley Sundquist

    Article

    Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

    Insert

    CHMP3
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-alpha

    Available since

    Oct. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLX208-SAE1

    Plasmid

    #171012

    Purpose

    Ectopic expression of SAE1

    Depositor

    Alice Ting

    Article

    Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

    Insert

    untagged SAE1
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1-alpha

    Available since

    Aug. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLX208-nfBFP

    Plasmid

    #171013

    Purpose

    Ectopic expression of nonfluorescent BFP

    Depositor

    Alice Ting

    Article

    Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

    Insert

    untagged nfBFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    BFP[H66A]

    Promoter

    Ef1-alpha

    Available since

    Aug. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDGB1a2+pNOS_Red-F_NOSt

    Plasmid

    #170887

    Purpose

    GoldenBraid Transcriptional Unit - pNOS_Red-F_NOSt Forward orientation

    Depositor

    Markita Landry

    Article

    Gonzalez-Grandio et al ACS Synth Biol. 2021 Sep 14. doi: 10.102

    Insert

    Red-F
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    p.S286Y Red mutant

    Promoter

    pNOS

    Available since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. p1.2-Zeo-hVKORC1

    Plasmid

    #162776

    Purpose

    Human vitamin K oxidoreductase expression

    Depositor

    Ivan Vorobiev

    Article

    Kovnir et al Acta Naturae. 2018 Jan-Mar;10(1):51-65.

    Insert

    VKORC1 vitamin K epoxide reductase complex subunit 1 (VKORC1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CHO EEF1A1 (Translation Elongation Factor 1 Alpha…

    Available since

    July 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pKB235

    Plasmid

    #170024

    Purpose

    Encodes LacI-controlled expression of surface-displayed CCL20 and PvirK-mNeonGreen reporter

    Depositor

    Jeffrey Tabor

    Article

    Brink et al bioRxiv

    Inserts

    mNeonGreen
    CCL20 (CCL20 Human, Escherichia coli, Synthetic)
    

    Use

    Synthetic Biology

    Tags

    Lpp, OmpA, and Tether

    Expression

    Bacterial

    Mutation

    Promoter

    Ptac and PvirK

    Available since

    July 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Tol2-aACry::EGFP-zebrafish Plaat1 (WT)

    Plasmid

    #168772

    Purpose

    Express EGFP-zebrafish Plaat1 (WT) in zebrafish lens

    Depositor

    Noboru Mizushima

    Article

    Morishita et al Nature. 2021 Apr;592(7855):634-638. doi:

    Insert

    Hrasls (plaat1 Zebrafish)
    

    Use

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    alphaA-crystallin promoter

    Available since

    May 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDONR221 Ub-NLRP1 L1215

    Plasmid

    #166828

    Purpose

    pDONR221 encoding ubiquitin-tagged NLRP1-CT to generate a L1215 N-terminus (no stop)

    Depositor

    Daniel Bachovchin

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Gateway shuttling vector

    Tags

    Ubiquitin

    Expression

    Mutation

    Promoter

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDONR221 Ub-NLRP1 L1218

    Plasmid

    #166829

    Purpose

    pDONR221 encoding ubiquitin-tagged NLRP1-CT to generate a L1218 N-terminus (no stop)

    Depositor

    Daniel Bachovchin

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Gateway shuttling vector

    Tags

    Ubiquitin

    Expression

    Mutation

    Promoter

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. dTAG NLRP1 P1214R

    Plasmid

    #166826

    Purpose

    dTAG (degron tag) NLRP1 for ligand-controlled N-terminal degradation

    Depositor

    Daniel Bachovchin

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    P1214R

    Promoter

    hPGK

    Available since

    March 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pHH0103_RARA_Hormone-recep

    Plasmid

    #109836

    Purpose

    Protein expression and purification of RARA_Hormone-recep

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    RARA_Hormone-recep (RARA Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pTCW018

    Plasmid

    #83531

    Purpose

    Aromatic amino acid inducible expression of alpha pheromone, and pheromone inducible exression of an ARO7 RNA interference hairpin

    Depositor

    Claudia Vickers

    Article

    Williams et al Metab Eng. 2015 May;29:124-134. doi: 10.

    Insert

    pARO9-mf_2-pFUS1J2-ARO7i-CYC1t
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    pARO9/pFUS1J2

    Available since

    Oct. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pEGB 1alfa1R Tnos:Hygromycin:PNos (GB0235)

    Plasmid

    #68213

    Purpose

    Transcriptional unit for Hygromycin resistance gene plant expression

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    Hyg
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Pnos

    Available since

    Oct. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pDGB1omega1_SF (GB0158)

    Plasmid

    #68242

    Purpose

    Twister plasmid to swap inserts from an omega2 or omega2R vector to any alpha level vector

    Depositor

    Diego Orzaez

    Article

    Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

    Insert

    Solanum lycopersicum intergenic region
    

    Use

    Synthetic Biology; Unspecified

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    Oct. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pEBB-GFP-hBEN-deltaSS

    Plasmid

    #22159

    Depositor

    Ananda Roy

    Article

    Tussie-Luna et al Proc Natl Acad Sci U S A. 2001 Jul 3. 98

    Insert

    hBEN (GTF2IRD1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Deletion of amino acids 897-908

    Promoter

    Available since

    Nov. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

27. pEBG-hBEN-deltaNLS3

    Plasmid

    #22161

    Depositor

    Ananda Roy

    Article

    Tussie-Luna et al Proc Natl Acad Sci U S A. 2001 Jul 3. 98

    Insert

    hBEN (GTF2IRD1 Human)
    

    Use

    Tags

    GST and His

    Expression

    Mammalian

    Mutation

    Deletion of amino acids 883-889

    Promoter

    Available since

    Nov. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

28. pEBG-hBEN-deltaSS

    Plasmid

    #22162

    Depositor

    Ananda Roy

    Article

    Tussie-Luna et al Proc Natl Acad Sci U S A. 2001 Jul 3. 98

    Insert

    hBEN (GTF2IRD1 Human)
    

    Use

    Tags

    GST and His

    Expression

    Mammalian

    Mutation

    Deletion of amino acids 897-908

    Promoter

    Available since

    Nov. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

29. 7xSMAD_RE::FLuc

    Plasmid

    #124531

    Purpose

    SMAD2 FLuc luciferase reporter

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

    Insert

    SMAD FLuc reporter
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. 7xE-Box::Renilla

    Plasmid

    #124532

    Purpose

    c-Myc Renilla luciferase reporter

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

    Insert

    c-Myc Renilla reporter
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pLKO5.sgRNA.EFS.GFP

    Plasmid

    #57822

    Purpose

    3rd generation lentiviral Vector for Sp sgRNA delivery without SpCas9, GFP, EFS Promoter driven

    Depositor

    Benjamin Ebert

    Article

    Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

    Inserts

    sgRNA
    EFS
    eGFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pcDNA3.1 6A NLRP1 CARD-FLAG WT

    Plasmid

    #163978

    Purpose

    Mammalian expression vector encoding human NLRP1 CARD with a C-terminal FLAG tag

    Depositor

    Hao Wu

    Article

    Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pT3-EF1a-c-Met

    Plasmid

    #31784

    Depositor

    Xin Chen

    Article

    Tward et al Proc Natl Acad Sci U S A. 2007 Sep 11;10

    Insert

    Met (MET Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    Oct. 21, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pmirGLO-3'UTR-TNF

    Plasmid

    #153069

    Purpose

    Human TNF 3'UTR region cloned downstream of luciferase reporter gene

    Depositor

    Silvia Monticelli

    Article

    Emming et al Nat Immunol. 2020 Apr;21(4):388-399. doi

    Insert

    3' UTR region of TNF gene (TNF Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    July 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. PB_tre_dCas9_KRAB

    Plasmid

    #126030

    Purpose

    PiggyBac cargo vector expressing doxycycline inducible dCas9-KRAB fusion

    Depositor

    Mauro Calabrese

    Article

    Schertzer et al RNA. 2019 May 17. pii: rna.068932.118. d

    Inserts

    dCas9 fusion with KRAB
    Hygromycin resistance
    

    Use

    CRISPR; Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A (catalytically inactive Cas9)

    Promoter

    EF1 alpha and TRE

    Available since

    June 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pEGFP-C2-NLRP3

    Plasmid

    #73955

    Purpose

    Express EGFP-tagged human NLRP3 in mammalian cells

    Depositor

    Christian Stehlik

    Article

    Khare et al Immunity. 2012 Mar 23;36(3):464-76. doi:

    Insert

    NLR family, pyrin domain containing 3 (NLRP3) (NLRP3 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV iE

    Available since

    April 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. PB_tre_dCas9_VP160

    Plasmid

    #126031

    Purpose

    PiggyBac cargo vector expressing doxycycline inducible dCas9-VP160 fusion

    Depositor

    Mauro Calabrese

    Article

    Schertzer et al RNA. 2019 May 17. pii: rna.068932.118. d

    Inserts

    dCas9 fusion with VP160
    Hygromycin resistance
    

    Use

    CRISPR; Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A (catalytically inactive Cas9)

    Promoter

    EF1 alpha and TRE

    Available since

    June 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pAcGFP1-N1-SialT

    Plasmid

    #87324

    Purpose

    To express SialT-AcGFP1 in mammalian cells, SialT encodes the full length coding sequence of β-galactosamide α-2,6-sialyltransferase

    Depositor

    Lei Lu

    Article

    Tie et al Mol Biol Cell. 2016 Jan 13. pii: mbc.E15

    Insert

    ST 6 beta-galactoside alpha-2,6-sialyltransferase 1 (ST6GAL1 Human)
    

    Use

    Tags

    AcGFP1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pX330-PITCh-TUBA1B

    Plasmid

    #207763

    Purpose

    Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the N-terminus of TUBA1B for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting N-terminus of TUBA1B (TUBA1B Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pLX_lenti_osTir1_9Myc_P2A_Bsd

    Plasmid

    #139746

    Purpose

    lentiviral vector expressing rice E3 ligase Tir1 in mammalian cells with selection marker

    Depositor

    Robert Tjian

    Article

    Xie et al Nat Methods. 2020 Apr;17(4):430-436. doi

    Insert

    rice E3 ligase Tir1 (osTir1)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. Apple-53BP1trunc

    Plasmid

    #69531

    Purpose

    Fluorescent reporter for DNA damage

    Depositor

    Ralph Weissleder

    Article

    Yang et al Sci Rep. 2015 May 18;5:10129. doi: 10.10

    Insert

    53BP1 (TP53BP1 Human)
    

    Use

    Lentiviral

    Tags

    Apple fluorescent protein

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. hCMV-IE1:Renilla

    Plasmid

    #118066

    Purpose

    Transcriptional unit for constitutive expression of Renilla in mammalian cells

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

    Insert

    CMV:GreenRenilla:bGHF
    

    Use

    Luciferase and Synthetic Biology; Assembly of gb2…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pLKO5.sgRNA.EFS.PAC

    Plasmid

    #57825

    Purpose

    Lentiviral Vector for Sp sgRNA delivery without SpCas9, Puromycin resistance, EFS Promoter driven

    Depositor

    Benjamin Ebert

    Article

    Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

    Inserts

    sgRNA
    EFS
    PAC
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pT/Caggs-NRASV12

    Plasmid

    #20205

    Depositor

    John Ohlfest

    Article

    Wiesner et al Cancer Res. 2009 Jan 15. 69(2):431-9.

    Insert

    NRas (NRAS Human)
    

    Use

    Transposon

    Tags

    Expression

    Mutation

    G12V

    Promoter

    Available since

    Feb. 4, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. piggyBAC-H2B FR-MQV

    Plasmid

    #194079

    Purpose

    Expresses nuclear-localized FR-MQV in mammalian cells

    Depositor

    Ralph Jimenez

    Article

    Mukherjee et al Biochemistry. 2020 Oct 6;59(39):3669-368

    Insert

    FR-MQV
    

    Use

    Tags

    Histone H2B

    Expression

    Mammalian

    Mutation

    Methionine 42 to Glutamine, Cystiene 159 to Valin…

    Promoter

    CMV7

    Available since

    Jan. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pLKO5.sgRNA.EFS.tRFP

    Plasmid

    #57823

    Purpose

    3rd generation Lentiviral Vector for Sp sgRNA delivery without SpCas9, tagRFP, EFS Promoter driven

    Depositor

    Benjamin Ebert

    Article

    Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

    Inserts

    sgRNA
    EFS
    tagRFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pCAGGS-PKCdelta-EGFP

    Plasmid

    #178856

    Purpose

    Mammalian expression of PKCδ fused to EGFP

    Depositor

    Shinya Tsukiji

    Article

    Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

    Insert

    PKCδ (PRKCD Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    July 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pQTEV-LRPAP1

    Plasmid

    #31327

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5. 4():21.

    Insert

    low density lipoprotein receptor-related protein associated (LRPAP1 Human)
    

    Use

    Tags

    His and TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pEF5B-FRT-control-APEX

    Plasmid

    #73187

    Purpose

    Expresses "control"-APEX (cytosolic) in mammalian cells

    Depositor

    Maxence Nachury

    Article

    Mick et al Dev Cell. 2015 Nov 23;35(4):497-512. doi

    Insert

    control-APEX (Nphp3 Glycine max, Mouse)
    

    Use

    Tags

    internal GFP tag

    Expression

    Mammalian

    Mutation

    changed glycine 2 to alanine

    Promoter

    EF1alpha

    Available since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pEBB Flag GCN5

    Plasmid

    #74784

    Purpose

    express of GCN5 in mammalian cells

    Depositor

    Ezra Burstein

    Article

    Mao et al Genes Dev. 2009 Apr 1;23(7):849-61. doi:

    Insert

    GCN5 (KAT2A Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1alpha promoter

    Available since

    June 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge