We narrowed to 13,721 results for: CRISPR-Cas9

Showing: 2281 - 2300 of 13721 results

Results per page:

Sort results by:

pAAV-pMecp2-dSaCas9-VP64-pU6-sgRNA

Plasmid

#158972

Purpose

Vector C encodes pAAV-pMecp2-dSaCas9-VP64-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of CRISPR activator in neurons

Depositor

Chung Tin

Article

Lau et al Mol Ther Nucleic Acids. 2019 Jun 7;16:63

Insert

dSaCas9-VP64

Use

AAV and CRISPR

Expression

Mammalian

Promoter

pMecp2

Available Since

Sept. 8, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-pMecp2-dSaCas9-KRAB-pU6-sgRNA

Plasmid

#158988

Purpose

Vector E encodes pAAV-pMecp2-dSaCas9-KRAB-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of CRISPR interference in neurons

Depositor

Chung Tin

Article

Lau et al Mol Ther Nucleic Acids. 2019 Jun 7;16:63

Insert

dSaCas9-KRAB

Use

AAV and CRISPR

Expression

Mammalian

Promoter

pMecp2

Available Since

Aug. 29, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-pMecp2-dSaCas9-VP160-pU6-sgRNA

Plasmid

#158987

Purpose

Vector D encodes pAAV-pMecp2-dSaCas9-VP160-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of CRISPR activator in neurons

Depositor

Chung Tin

Article

Lau et al Mol Ther Nucleic Acids. 2019 Jun 7;16:63

Insert

dSaCas9-VP160

Use

AAV and CRISPR

Expression

Mammalian

Promoter

pMecp2

Available Since

Aug. 29, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-pMecp2-dSaCas9-SID4X-pU6-sgRNA

Plasmid

#158989

Purpose

Vector F encodes pAAV-pMecp2-dSaCas9-SID4X-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of CRISPR interference in neurons

Depositor

Chung Tin

Article

Lau et al Mol Ther Nucleic Acids. 2019 Jun 7;16:63

Insert

dSaCas9-SID4X

Use

AAV and CRISPR

Expression

Mammalian

Promoter

pMecp2

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCSDest2-SpCas9-WT-NLS-3XHA-NLS-ZFP_TS4

Plasmid

#69227

Purpose

Expresses wild type SpCas9 fused to ZFP-TS4 in mammalian cells

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

Insert

TS4 ZFP

Use

CRISPR

Tags

NLS-3XHA-NLS

Expression

Mammalian

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pCSDest2-SpCas9-WT-NLS-3XHA-NLS-ZFP_TS2

Plasmid

#69225

Purpose

Expresses wild type SpCas9 fused to ZFP-TS2 in mammalian cells

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

Insert

TS2 ZFP

Use

CRISPR

Tags

NLS-3XHA-NLS

Expression

Mammalian

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pCSDest2-SpCas9-WT-NLS-3XHA-NLS-ZFP_TS3

Plasmid

#69226

Purpose

Expresses wild type SpCas9 fused to ZFP-TS3 in mammalian cells

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

Insert

TS3 ZFP

Use

CRISPR

Tags

NLS-3XHA-NLS

Expression

Mammalian

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
PX458-3xHA-en31FnCas9

Plasmid

#201949

Purpose

Mammalian expression plasmid of enhanced activity FnCas9 variant en31FnCas9 with T2A-EGFP and cloning backbone for sgRNA

Depositor

Debojyoti Chakraborty

Article

Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

Insert

3xHA-NLS-en31FnCas9-T2A-EGFP

Use

CRISPR

Tags

3xHA, NLS, and T2A-EGFP

Expression

Mammalian

Mutation

G1243T, E1369R, E1603H on FnCas9

Promoter

Cbh

Available Since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
PX458-3xHA-en1FnCas9

Plasmid

#201947

Purpose

Mammalian expression plasmid of enhanced activity FnCas9 variant en1FnCas9 with T2A-EGFP and cloning backbone for sgRNA

Depositor

Debojyoti Chakraborty

Article

Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

Insert

3xHA-NLS-en1FnCas9-T2A-EGFP

Use

CRISPR

Tags

3xHA, NLS, and T2A-EGFP

Expression

Mammalian

Mutation

E1369R on FnCas9

Promoter

Cbh

Available Since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
PX458-3xHA-en15FnCas9

Plasmid

#201948

Purpose

Mammalian expression plasmid of enhanced activity FnCas9 variant en15FnCas9 with T2A-EGFP and cloning backbone for sgRNA

Depositor

Debojyoti Chakraborty

Article

Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

Insert

3xHA-NLS-en15FnCas9-T2A-EGFP

Use

CRISPR

Tags

3xHA, NLS, and T2A-EGFP

Expression

Mammalian

Mutation

E1603H on FnCas9

Promoter

Cbh

Available Since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
PX458-3xHA-FnCas9ABEmax8.17d

Plasmid

#201955

Purpose

Mammalian expression plasmid of FnCas9ABEmax8.17d base editor with T2A-EGFP and cloning backbone for sgRNA

Depositor

Debojyoti Chakraborty

Article

Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

Insert

bpNLS-FnCas9ABEmax8.17d-bpNLS-3xHA-T2A-EGFP

Use

CRISPR

Tags

3xHA, NLS, and T2A-EGFP

Expression

Mammalian

Mutation

D11A on FnCas9, V82S, V106W, Q154R on mutant TadA

Promoter

Cbh

Available Since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
SIN-cPPT-G1B3-spCas9-WPRE-miR124T

Plasmid

#245071

Purpose

CRISPR Editing with SpCas9, with miRNA-124 target sequence to repress transgene expression in neurons

Depositor

Nicole Déglon

Insert

Cas9, miR124T site

Use

Lentiviral

Expression

Mammalian

Promoter

Human G1B3 promoter

Available Since

Dec. 18, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
lenti-EF1a-dCas9-KRAB-Puro

Plasmid

#99372

Purpose

3rd generation lenti vector encoding dCas9-KRAB with 2A puromycin resistance marker (EF1a-dCas9-KRAB-T2A-Puro-WPRE)

Depositor

Kristen Brennand

Article

Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

Insert

dCas9-KRAB-T2A-Puro

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF-1a

Available Since

Aug. 18, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pU6-ACTA2_gRNA1-SpCas9-T2A-GFP

Plasmid

#126706

Purpose

to express Cas9 from S. pyogenes with 2A-EGFP and sgRNA targeting human ACTA2 locus at the end of the endogenous coding sequence

Depositor

Darrell Kotton

Article

Kwong et al Stem Cell Reports. 2019 Sep 10;13(3):499

Insert

ACTA2_gRNA1-SpCas9-T2A-GF (ACTA2 Human)

Use

CRISPR

Available Since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
lenti-EF1a-dCas9-VP64-Puro

Plasmid

#99371

Purpose

3rd generation lenti vector encoding dCas9-VP64 with 2A puromycin resistance marker (EF1a-dCas9-VP64-T2A-Puro-WPRE)

Depositor

Kristen Brennand

Article

Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6

Insert

dCas9-VP64-T2A-Puro

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF-1a

Available Since

Aug. 18, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pACYC-T7-SpCas9-T7-EGFP_gRNA1-T7term (BPK848)

Plasmid

#181745

Purpose

dual T7 promoter vector for bacterial expression of SpCas9 with a c-terminal NLS and 3xFLAG tag, along with an SpCas9 sgRNA targeting EGFP site 1

Depositor

Benjamin Kleinstiver

Article

Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038

Insert

human/zebrafish codon optimized SpCas9

Use

In vitro transcription; t7 promoter

Tags

NLS(SV40)-3xFLAG

Expression

Bacterial

Promoter

T7

Available Since

Feb. 16, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLL7 lenti-dcas9-3xflag-blast

Plasmid

#112133

Purpose

lenti vector encoding dcas9-3xflag with T2A Blastcidin resistance marker (EF1a-NLS-dCas9-3Xflag-T2A-Blast-WPRE)

Depositor

Fei-Long Meng

Article

Liu et al Cell Rep. 2018 Oct 23;25(4):884-892.e3.

Insert

dCas9(D10A, N863A)-T2A-Blast

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D10A and N863A mutants in Cas9

Promoter

EF1a

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
PET-B-Sniper SpCas9-NLS-6xHis

Plasmid

#207377

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-Sniper SpCas9

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

F539S, M763I, K890N, amino acids 1005-1013 replac…

Promoter

T7

Available Since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pSH231-EF1-BLST-dCas9-VPR

Plasmid

#115148

Purpose

Safe harbor site 231 knock-in vector with BlastR-dCas9-VPR expression cassette

Depositor

Raymond Monnat, Michael Phelps

Article

Pellenz et al Hum Gene Ther. 2019 Jul;30(7):814-828. d

Insert

BlastR-P2A-dCas9-VPR

Use

CRISPR

Tags

Nucleoplasmin NLS and SV40NLS

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV hUbC-dCas9-dMQ1-EGFG

Plasmid

#89794

Purpose

Targeted CpG methylation

Depositor

Margaret Goodell

Article

Lei et al Nat Commun. 2017 Jul 11;8:16026. doi: 10

Insert

site-specific DNA-methyltransferase SssI

Use

CRISPR and Lentiviral

Tags

3*FLAG and T2A-EGFP

Expression

Mammalian

Mutation

C141S, TGC-->TCC; S317A, AGC-->GCC

Promoter

hUbC

Available Since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge