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We narrowed to 6,530 results for: nls

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Showing: 4601 - 4640 of 6530 results

  2. pGreenIIM DR5v2-ntdTomato/DR5-n3GFP

    Plasmid
    #61628
    Purpose
    Expresses nuclear tdTomato from auxin-dependent DR5v2 (TGTCGG) promoter and nuclear 3xGFP from the DR5 (TGTCTC) promoter
    Depositor
    Dolf Weijers
    Article
    Liao et al Nat Methods. 2015 Mar;12(3):207-10. doi:
    Inserts
    ntdTomato
    n2xEGFP
    Expression
    Plant
    Mutation
    2 copies of EGFP with N-terminal SV40 NLS
    Promoter
    Auxin-dependent DR5 promoter containing 9 reverse…
    Available Since
    March 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    61628-plasmid-map-sequence-id-396550

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  10. dCas9-hHDAC3

    Plasmid
    #98591
    Purpose
    3rd generation lenti vector encoding dCas9-hHDAC3 (EF1a-NLS-dCas9(N863)-hHDAC3). Expresses dCas9 fused to human HDAC3.
    Depositor
    Zhaolan Zhou
    Article
    Kwon et al Nat Commun. 2017 May 12;8:15315. doi: 10
    Insert
    dCAS9-HDAC3 (HDAC3 Synthetic, S. pyogenes, Human)
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A and N863A in Cas9
    Promoter
    EF1A
    Available Since
    Oct. 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    98591-plasmid-map-sequence-id-339879

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  11. pBG-ttLbUV2

    Plasmid
    #231386
    Purpose
    LbCas12a variant with D156R and E795L mutations and harboring same NLS sequence as PEmax but codon-optimized for dicot species; for Agrobacterium-mediated Arabidopsis transformation.
    Depositor
    Qi-Jun Chen
    Article
    Xin et al aBIOTECH. 2024 Mar 26;5(2):117-126. doi:
    Insert
    ttLbCas12a Ultra V2
    Use
    CRISPR
    Expression
    Plant
    Mutation
    D156R and E795L
    Available Since
    Feb. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    231386-plasmid-map-sequence-id-461803

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  14. BB3cK_pGAP_23*_pTEF_Cas9

    Plasmid
    #104909
    Purpose
    hCas9 under control of Tef1 for direct cloning of HH-sgRNA-HDV PCR products and episomal expression in P. pastoris and G418 selection
    Depositor
    Brigitte Gasser
    Article
    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi
    Inserts
    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    Use
    CRISPR
    Expression
    Yeast
    Available Since
    June 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    104909-plasmid-map-sequence-id-206071

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  23. pLV-REPORT(PGK/CMV)

    Plasmid
    #172330
    Purpose
    LV REPORT containing minimal promoter driving monomeric nuclear Tomato 2a HSVtk 2a Neo followed by an PGK/CMV promoter driving nuclear d2eGFP E2A Hygromycin
    Depositor
    David Bersten
    Article
    Allen et al bioRxiv 2023.11.21.568191
    Inserts
    monomeric nuclear Tomato
    d2eGFP
    HygR
    Use
    Lentiviral and Synthetic Biology
    Tags
    3x SV40 NLS
    Expression
    Mammalian
    Promoter
    PGK/CMV
    Available Since
    June 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    172330-plasmid-map-sequence-id-497743

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  24. BB3cK_pGAP_23*_pPFK300_Cas9

    Plasmid
    #104910
    Purpose
    hCas9 under control of pPFK300 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on G418
    Depositor
    Brigitte Gasser
    Article
    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi
    Inserts
    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    Use
    CRISPR
    Expression
    Yeast
    Available Since
    April 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    104910-plasmid-map-sequence-id-206061

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  29. BB3cK_pGAP_23*_pLAT1_Cas9

    Plasmid
    #104908
    Purpose
    hCas9 under control of LAT1 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on G418
    Depositor
    Brigitte Gasser
    Article
    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi
    Inserts
    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    Use
    CRISPR
    Expression
    Yeast
    Available Since
    Oct. 1, 2019
    Availability
    Academic Institutions and Nonprofits only
    104908-plasmid-map-sequence-id-206067

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  33. pYPQ230-RRV

    Plasmid
    #188543
    Purpose
    Gateway entry clone (attL1 & attR5) for LbCas12a-RRV mediated genome editing
    Depositor
    Yiping Qi
    Article
    Zhang et al Genome Biol. 2023 Apr 30;24(1):102. doi:
    Insert
    LbCas12a-RRV
    Use
    CRISPR; Gateway compatible lbcas12a-rrv entry clo…
    Tags
    NLS
    Expression
    Plant
    Mutation
    G146R, D156R, R182V
    Available Since
    May 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    188543-plasmid-map-sequence-id-369473

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  34. pHS1993

    Plasmid
    #239836
    Purpose
    OMEGAOff-KRK mammalian expression
    Depositor
    Feng Zhang
    Article
    Kannan et al Nat Biotechnol. 2025 May 7. doi: 10.1038
    Insert
    DNMT3A-DNMT3L-OrufIscB-KRK-KRAB
    Tags
    HA and SV40 NLS
    Expression
    Mammalian
    Mutation
    Insertion of REC domain from NbaCas9-1 + E137K/E4…
    Promoter
    CMV
    Available Since
    July 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    239836-plasmid-map-sequence-id-479108

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  35. dpYPQ230-D156R-ABE8

    Plasmid
    #195569
    Purpose
    dLbCas12a-D156R based adenine base editor, ecTadA8e fused to the N terminal of dLbCas12a-D156R with the linker of (GSAGSAAGSGEF)x3
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Commun. 2023 Apr 13:100601. doi: 1
    Insert
    ecTadA8e-(GSAGSAAGSGEF)x3-dLbCas12a-D156R
    Use
    CRISPR; Gateway compatible ectada8e-(gsagsaagsgef…
    Tags
    5' and 3' NLS
    Expression
    Plant
    Promoter
    ZmUBI1
    Available Since
    May 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    195569-plasmid-map-sequence-id-392989

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  36. pYPQ230-RRVQ

    Plasmid
    #200188
    Purpose
    Gateway entry clone (attL1 & attR5) for LbCas12a-RRVQ mediated genome editing
    Depositor
    Yiping Qi
    Article
    Zhang et al Genome Biol. 2023 Apr 30;24(1):102. doi:
    Insert
    LbCas12a-RRVQ
    Use
    CRISPR; Gateway compatible lbcas12a-rrv entry clo…
    Tags
    NLS
    Expression
    Plant
    Mutation
    G146R, D156R, R182V, E795Q
    Available Since
    June 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    200188-plasmid-map-sequence-id-396764

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  37. pLV-REPORT(EF1a)-TTN

    Plasmid
    #172327
    Purpose
    LV REPORT containing minimal promoter driving monomeric nuclear Tomato 2a HSVtk 2a Neo followed by an EF1a promoter driving nuclear d2eGFP E2A Hygromycin
    Depositor
    David Bersten
    Article
    Allen et al bioRxiv 2023.11.21.568191
    Inserts
    monomeric nuclear Tomato
    d2eGFP
    HygR
    Use
    Lentiviral and Synthetic Biology
    Tags
    3x SV40 NLS
    Expression
    Mammalian
    Promoter
    EF1-alpha
    Available Since
    June 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    172327-plasmid-map-sequence-id-354684

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  38. pUBC_stdMCP_serinemod_E488QADAR_p2A_yGFP

    Plasmid
    #154787
    Purpose
    Expresses synonymized MS2 coat protein dimer (MCP) fused to hyperactive ADAR catalytic domain
    Depositor
    Robert Singer
    Article
    Biswas et al iScience. 2020 Jun 28;23(7):101318. doi:
    Insert
    Adenosine deaminse (Adar Synthetic, Fly)
    Use
    Lentiviral
    Tags
    GFP, V5, and stdMCP
    Expression
    Mammalian
    Mutation
    Synonymized serine to prevent autoediting of ADAR…
    Promoter
    UBC
    Available Since
    July 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    154787-plasmid-map-sequence-id-306329

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  40. pGfa2-nLac

    Plasmid
    #53126
    Purpose
    Expression of LacZ driven by the human Gfa2 promoter. LacZ can also be replaced with the gene of interest for targeting transgene expression to astrocytes in mice.
    Depositor
    Michael Brenner
    Article
    Brenner et al J Neurosci. 1994 Mar;14(3 Pt 1):1030-7.
    Insert
    Gfa2 (GFAP Human)
    Use
    Mouse Targeting
    Tags
    LacZ, NLS, and mP1
    Expression
    Mammalian
    Mutation
    contains -2163 to +47 (the human gfa2 segment), w…
    Promoter
    human GFAP
    Available Since
    May 8, 2014
    Availability
    Academic Institutions and Nonprofits only
    53126-plasmid-map-sequence-id-82755

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Showing: 4601 - 4640 of 6530 results