We narrowed to 15,043 results for: NOL

1. Lenti-Trono-EFS-Blast-P2A-TurboRFP

   Plasmid

   #228893

   Purpose

   Cloning and expression of gRNAs or pegRNAs with EcoRI/Esp3I insertion.

   Depositor

   Francisco J Sánchez-Rivera

   Article

   Gould et al Nat Biotechnol. 2024 Mar 12. doi: 10.103

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Rc/CMV cyclin E

   Plasmid

   #8963

   Depositor

   Bob Weinberg

   Article

   Hinds et al Cell. 1992 Sep 18. 70(6):993-1006.

   Insert

   cyclin E (CCNE1 Human)
   

   Use

   Tags

   GPI linker

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 5, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCoofy55

   Plasmid

   #122007

   Purpose

   Mammalian vector for parallel SLIC cloning containing N-terminal His10-SumoStar (Life Sensors) with additional PreScission Cleavage site and different C-tag options

   Depositor

   Sabine Suppmann

   Article

   Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

   Type

   Empty backbone

   Use

   Tags

   His10-SumoStar (Life Sensors) and Multi2: dependi…

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. GFP-TREX1(D18N)

   Plasmid

   #27220

   Depositor

   Judy Lieberman

   Article

   Yan et al Nat Immunol. 2010 Nov . 11(11):1005-13.

   Insert

   TREX1 (TREX1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   D18N sequence 52-GAC changed to AAC, resulting i…

   Promoter

   Available since

   Jan. 26, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. nLbCas12a

   Plasmid

   #213745

   Purpose

   For circular RNA-mediated prime editor using nLbCas12a-D156R-R1138A in HEK293T cells

   Depositor

   Caixia Gao

   Article

   Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

   Insert

   nLbCas12a
   

   Use

   CRISPR

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   D156R, R1138A

   Promoter

   CMV

   Available since

   Feb. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pFA6a-mCherry-sfGFP-kanMX (pMaM17)

   Plasmid

   #173452

   Purpose

   Contains mCherry-sfGFP tandem fluorescent protein timer

   Depositor

   Anton Khmelinskii

   Article

   Khmelinskii et al Nat Biotechnol. 2012 Jun 24;30(7):708-14

   Insert

   mCherry-sfGFP-ADH1ter
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   no promoter

   Available since

   Sept. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBbS8c-ddcpf1-rfp

   Plasmid

   #153038

   Purpose

   For CRISPRi. Arabinose-inducible ddcpf1 gene with CRISPR array targeting the mrfp reporter gene.

   Depositor

   Nigel Scrutton

   Article

   Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.

   Insert

   Ascpf1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   E993A

   Promoter

   Available since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pOCC5

   Plasmid

   #118863

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C protease

   Depositor

   PEPC MPI CBG, Marc Bickle

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-HIS6-3C-NotI-ccdB-AscI-stop-HindIII cassette
   

   Use

   Tags

   HIS6, cleavable with 3C protease

   Expression

   Insect

   Mutation

   Promoter

   polH

   Available since

   Jan. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDEST-hisMBP-AsCpf1-EC

   Plasmid

   #79007

   Purpose

   Expressing his-MBP tagged AsCpf1 (E.coli codon optimized)

   Depositor

   Jin-Soo Kim

   Article

   Hur et al Nat Biotechnol. 2016 Jun 6. doi: 10.1038

   Insert

   AsCpf1
   

   Use

   Tags

   6xHis-MBP-NLS and NLS-HA

   Expression

   Bacterial

   Mutation

   Promoter

   tac promoter

   Available since

   July 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. GI-Gal4 DBD

    Plasmid

    #42500

    Purpose

    GIGANTEA fused with Gal4 DNA binding domain (DBD), part of a light-inducible transcription factor

    Depositor

    Ricardo Dolmetsch

    Article

    Yazawa et al Nat Biotechnol. 2009 Oct;27(10):941-5. d

    Insert

    GIGANTEA fused with Gal4 DBD
    

    Use

    Tags

    Gal4 DNA binding domain (DBD)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBABE-puro-DN-hTERT

    Plasmid

    #1775

    Depositor

    Bob Weinberg

    Article

    Hahn et al Nat Med 1999 Oct;5(10):1164-70.

    Insert

    hTERT (TERT Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Dominant negative, 2 base pair mutation, catalyti…

    Promoter

    Available since

    June 23, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMM114

    Plasmid

    #127211

    Purpose

    BeYDV replicon with constitutive Luc expression, WUS2 and IPT, sgRNA targeting PDS

    Depositor

    Daniel Voytas

    Article

    Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

    Insert

    WUS2, IPT, sgRNA, Luc
    

    Use

    Tags

    Expression

    Plant

    Mutation

    codon optimized CDSs

    Promoter

    Available since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. PGAL

    Plasmid

    #60337

    Purpose

    Contains full length native galactose promoter

    Depositor

    Hal Alper

    Article

    Blazeck et al Biotechnol Bioeng. 2012 Nov;109(11):2884

    Inserts

    promoter
    yECitrine
    

    Use

    Synthetic Biology; Yeast synthetic hybrid promote…

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 6, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pJIT163-AFID-3

    Plasmid

    #167359

    Purpose

    For inducing predictable deletions in protoplasts and wheat plants

    Depositor

    Caixia Gao

    Article

    Wang et al Nat Biotechnol. 2020 Dec;38(12):1460-146

    Insert

    A3A-Cas9-UDG-T2A-AP lyase
    

    Use

    Tags

    Expression

    Plant

    Mutation

    WT

    Promoter

    Available since

    March 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. HERV-Kcon-RT for Split-PE (pJUL3120)

    Plasmid

    #190107

    Purpose

    Expresses HERV-Kcon RT (human codon optimization)

    Depositor

    Keith Joung

    Article

    Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

    Insert

    HERV-Kcon-RT
    

    Use

    Tags

    bpNLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDAI-SceI-pheS

    Plasmid

    #111611

    Purpose

    Yeast homing endonuclease I - SceI expression vector. Used for resolution of merodiploids during allelic exchange in Burkholderia sp.

    Depositor

    Tim Tolker-Nielsen

    Article

    Fazli et al Appl Environ Microbiol. 2015 Jun;81(11):

    Insert

    pheS
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFBD-SpyTag-noro-VLP

    Plasmid

    #165989

    Purpose

    Expresses norovirus-like particles with SpyTags presented on their surface for convenient decoration of SpyCatcher fusion proteins

    Depositor

    Vesa Hytönen

    Article

    Lampinen et al J Nanobiotechnology. 2021 Jan 19;19(1):2

    Insert

    SpyTag-noro-VLP (ORF2 Synthetic, norovirus)
    

    Use

    Tags

    SpyTag

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    March 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJBL711

    Plasmid

    #140381

    Purpose

    Expresses OtrR protein under the control of T7-lacO promoter

    Depositor

    Julius Lucks

    Article

    Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

    Insert

    T7-lacO-OtrR-T
    

    Use

    Tags

    TEV-6XHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCR4 hNFATc1-EE

    Plasmid

    #17857

    Depositor

    Jerry Crabtree

    Article

    Northrop et al Nature. 1994 Jun 9. 369(6480):497-502.

    Insert

    NFATc1 (NFATC1 Human)
    

    Use

    Tags

    EE

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 29, 2008

    Availability

    Academic Institutions and Nonprofits only

20. pTH1-SaSrtA

    Plasmid

    #64973

    Purpose

    Expresses the wild type of Staphylococcus aureus sortase A in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

    Insert

    sortase A
    

    Use

    Tags

    His6 tag, Mxe GyrA intein, and chitin-binding pro…

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-59

    Promoter

    T7 promoter

    Available since

    July 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. Sth1Cas9n cytosine base editor

    Plasmid

    #194209

    Purpose

    Sth1Cas9-D9A-nickase_rAPOBEC1_cytosine base editor for RNA recording

    Depositor

    Chase Beisel

    Article

    Jiao et al Nat Biotechnol. 2023 Jan 5. doi: 10.1038

    Insert

    Sth1Cas9-D9A nickase
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Changed Aspartate 9 to Alanine.

    Promoter

    Available since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pUC19/mChe-Px

    Plasmid

    #183170

    Purpose

    Construct for transient expression of peroxisomes-targeted mCherry in plants.

    Depositor

    Yao-Guang Liu, Qinlong Zhu

    Article

    Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

    Insert

    mCherry-Px
    

    Use

    Tags

    10 aa peroxisome targeting signal 1 (IHHPRELSRL) …

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pMK345 (Hygro-P2A-mAID-mClover)

    Plasmid

    #121179

    Purpose

    N-terminal tagging

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

    Insert

    Hygro-P2A-mAID-mClover
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pUC19/Pd-mChe

    Plasmid

    #183172

    Purpose

    Construct for transient expression of plasmodesmata-targeted mCherry in plants.

    Depositor

    Yao-Guang Liu, Qinlong Zhu

    Article

    Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

    Insert

    Pd-mCherry
    

    Use

    Tags

    AtPDCB1, A. thaliana PLASMODESMATA CALLOSE-BINDIN…

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pSL4393 (pClpX_Eco)

    Plasmid

    #200907

    Purpose

    Expresses human codon-optimized E. coli ClpX from a CMV promoter.

    Depositor

    Samuel H. Sternberg

    Article

    Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

    Insert

    EcoClpX
    

    Use

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. GB4080

    Plasmid

    #215249

    Purpose

    Module for the constitutive expression of EGFP, p19 and the N. nambi luminescence pathway genes fused to a SF.

    Depositor

    Diego Orzaez

    Article

    Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

    Insert

    SF-EGFP-p19-LUZ-H3H-HISPS-CPH
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    March 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pUC19/mChe-Cs

    Plasmid

    #183171

    Purpose

    Construct for transient expression of cytoskeleton-targeted mCherry in plants.

    Depositor

    Yao-Guang Liu, Qinlong Zhu

    Article

    Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

    Insert

    mCherry-Cs
    

    Use

    Tags

    363 aa of A. thaliana FIMBRIN 1 (AtFIM1)

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pB-nCas9-PBE

    Plasmid

    #98160

    Purpose

    Expresses CRISPR base editor in maize

    Depositor

    Caixia Gao

    Article

    Zong et al Nat Biotechnol. 2017 May;35(5):438-440.

    Insert

    NLS-APOBEC1-XTEN-nCas9-UGI-NLS
    

    Use

    Maize

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Ubi

    Available since

    Aug. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pBBRBB-eGFP

    Plasmid

    #32549

    Depositor

    Claudia Schmidt-dannert

    Article

    Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

    Insert

    eGFP (eGFP )
    

    Use

    Synthetic Biology

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Consitutive lac

    Available since

    Nov. 8, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pBT2dR3-proB

    Plasmid

    #122595

    Purpose

    BE PACE selection, express T7 RNA polymerase with degron-GTCCA target

    Depositor

    David Liu

    Article

    Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

    Insert

    T7 RNA polymerase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. Circular guide cloning vector

    Plasmid

    #180184

    Purpose

    Cloning vector for expressing circular RNA from a U6 promoter

    Depositor

    Prashant Mali

    Article

    Katrekar et al Nat Biotechnol. 2022 Feb 10. pii: 10.103

    Type

    Empty backbone

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pUC19/CF-mChe

    Plasmid

    #183166

    Purpose

    Construct for transient expression of cytoplasmic foci-targeted mCherry in plants.

    Depositor

    Yao-Guang Liu, Qinlong Zhu

    Article

    Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

    Insert

    CF-mCherry
    

    Use

    Tags

    AtTZF1, A. thaliana TANDEM ZINC FINGER PROTEIN 1

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pUC19/Tp-mChe

    Plasmid

    #183165

    Purpose

    Construct for transient expression of tonoplast-targeted mCherry in plants.

    Depositor

    Yao-Guang Liu, Qinlong Zhu

    Article

    Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

    Insert

    Tp-mCherry
    

    Use

    Tags

    28 aa of A. thaliana CALCINEURIN B-LIKE PROTEIN 2…

    Expression

    Plant

    Mutation

    Promoter

    Available since

    April 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pMM113

    Plasmid

    #127210

    Purpose

    BeYDV replicon with constitutive Luc expression, WUS2 and AtSTM, sgRNA targeting PDS

    Depositor

    Daniel Voytas

    Article

    Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

    Insert

    WUS2, AtSTM, sgRNA, Luc
    

    Use

    Tags

    Expression

    Plant

    Mutation

    codon optimized CDSs

    Promoter

    Available since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pSL3713 (pTnsC_Pse)

    Plasmid

    #200900

    Purpose

    Expresses human codon-optimized Pseudoalteromonas TnsC from a CMV promoter.

    Depositor

    Samuel H. Sternberg

    Article

    Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

    Insert

    PseTnsC
    

    Use

    Tags

    3xNLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pMM134

    Plasmid

    #127215

    Purpose

    BeYDV replicon with constitutive Luc expression, IPT, sgRNA targeting PDS

    Depositor

    Daniel Voytas

    Article

    Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

    Insert

    Luc, IPT, sgRNA
    

    Use

    Tags

    Expression

    Plant

    Mutation

    codon optimized CDSs

    Promoter

    Available since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. sgLenti

    Plasmid

    #105996

    Purpose

    single sgRNA expression vector

    Depositor

    Mike McManus

    Article

    Boettcher et al Nat Biotechnol. 2018 Jan 15. pii: nbt.40

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral; S.pyogenes sgrna expressio…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pHBintE

    Plasmid

    #48136

    Purpose

    Shuttle vector E. coli/B.meg., carrying a temperature sensitive origin of replication for Bacillus megaterium, selection marker erytromycine for B. megaterium, integration vector

    Depositor

    Dieter Jahn

    Article

    Biedendieck et al Microb Biotechnol. 2010 Jan;3(1):24-37.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 21, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. EasyFusion Halo-mAID

    Plasmid

    #112852

    Purpose

    General donor vector with Halo-mAID coding sequence flanking by restriction sites for generating knock-in donor for Auxin inducible degradation of Halo tagged endogenous protein

    Depositor

    Janet Rossant

    Article

    Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

    Insert

    Halo-mAID
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    No

    Available since

    July 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pBT120b

    Plasmid

    #122604

    Purpose

    BE PACE selection, express gIII, luxAB and degron-GTCCA guide RNA

    Depositor

    David Liu

    Article

    Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

    Insert

    gIII, luxAB, guide RNA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. ND4.2-Left TALE-G1397-N-wildtype

    Plasmid

    #179684

    Purpose

    Expression of base editor in mammalian cells

    Depositor

    David Liu

    Article

    Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

    Insert

    COX8a MTS_3xFLAG_ND4.2 Left TALE_2aa_DddA G1397-N_1xUGI_P2A_mCherry_ATP5B 3'UTR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pBT13-splitD

    Plasmid

    #122597

    Purpose

    clone M13 phage by SapI Golden Gate

    Depositor

    David Liu

    Article

    Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

    Insert

    M13 bacteriophage genes
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pEC2861 (pSpy1C-PgyrA_ffluc)

    Plasmid

    #218511

    Purpose

    replicative E. coli-S. pyogenes shuttle plasmid, constitutive expression of firefly luciferase reporter

    Depositor

    Emmanuelle Charpentier

    Article

    Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

    Insert

    ffluc
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    deletion of Plac_mrfp cassette

    Promoter

    PgyrA

    Available since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pCDNA3-LOV-VP16

    Plasmid

    #42499

    Purpose

    LOV (N terminal domain of FKF1) fused to VP16, part of a light-inducible transcription factor

    Depositor

    Ricardo Dolmetsch

    Article

    Yazawa et al Nat Biotechnol. 2009 Oct;27(10):941-5. d

    Insert

    FKF1 (N-LOV, G128D mutant) fused with VP16
    

    Use

    Tags

    F-type VP16 activation domain and HA tag

    Expression

    Mammalian

    Mutation

    G128D 1-168aa

    Promoter

    CMV

    Available since

    March 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. IVT-nCas9(H840A)

    Plasmid

    #207455

    Purpose

    in vitro transcription of mRNA

    Depositor

    Erik Sontheimer

    Article

    Liu et al Nat Biotechnol. 2023 Sep 14. doi: 10.103

    Insert

    H840A
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pMK349 (BSD-P2A-mAID-mCherry)

    Plasmid

    #121183

    Purpose

    N-terminal tagging

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

    Insert

    BSD-P2A-mAID-mCherry
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. pBT44c

    Plasmid

    #122600

    Purpose

    BE PACE selection, express C-intein-Cas9-ugi fusion

    Depositor

    David Liu

    Article

    Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

    Insert

    intein-dCas9-ugi fusion
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. ND4.2-Right DdCBE-G1397-C-wildtype

    Plasmid

    #179687

    Purpose

    Expression of base editor in mammalian cells

    Depositor

    David Liu

    Article

    Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

    Insert

    SOD2 MTS_3xHA_ND4.2 Right TALE_2aa_DddA G1397-C )_1xUGI_SOD2 3'UTR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pR26 iBFP Dest

    Plasmid

    #74284

    Purpose

    Gene targeting vector for the mouse Rosa26 locus, including a loxP flanked stop cassette and BFP, for gateway cloning

    Depositor

    Ralf Kuehn

    Article

    Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

    Insert

    Rosa26 5-homology region
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pMM119

    Plasmid

    #127213

    Purpose

    BeYDV replicon with constitutive GFP expression, WUS2, sgRNA targeting PDS

    Depositor

    Daniel Voytas

    Article

    Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

    Insert

    GFP, WUS2, sgRNA
    

    Use

    Tags

    Expression

    Plant

    Mutation

    codon optimized CDSs

    Promoter

    Available since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge