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pLV UBC msNLRP3(LRRm2)-APEX2-3xFLAG-IRES-eGFP (N1008R_R1009E_E1010R_R1013E)

Plasmid

#218635

Purpose

Proximity biotinylation of msNLRP3(LRRm2, N1008R_R1009E_E1010R_R1013E)

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

NLRP3 (Nlrp3 Mouse)

Use

Lentiviral

Tags

APEX2-3xFLAG

Expression

Mammalian

Mutation

N1008R, R1009E, E1010R, R1013E

Promoter

UBC

Available since

May 1, 2024

Availability

Academic Institutions and Nonprofits only

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pmCherry2-GNB1-T2A-mCherry2-GNG2-IRES-GNAI1-mEYFP(Q69K)

Plasmid

#190755

Purpose

Expression of trimeric G protein with mCherry2 (GNB1 and GNG2) or mEYFP(Q69K) (GNAI1) tags.

Depositor

Dave Piston

Article

Foust et al bioRxiv 2021.11.03.467169

Inserts

GNB1 (GNB1 Human)
GNG2 (GNG2 Human)
GNAI1 (GNAI1 Human)

Use

Tags

mCherry2 and mEYFP(Q69K)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 18, 2022

Availability

Academic Institutions and Nonprofits only

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AiP1070-Ai211 (TITL-ChrimsonR-tdT-Kv2.1-ICF-IRES-tTA2)

Plasmid

#181764

Purpose

targeting vector

Depositor

Tanya Daigle

Insert

Kv2.1-tagged ChrimsonR-tdTomato and tTA2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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pRDAX-491 GFP[1] L3 Stuffer/Intein3 L4 GFP[2] IRES mCherry GFP(1-9)

Plasmid

#130174

Purpose

PPI (BiFC/TriFC) assay vector

Depositor

Gary Lewin

Insert

GFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pRDAX-490 GFP[1] L3 Stuffer/Intein2 L4 GFP[2] IRES mCherry GFP(1-9)

Plasmid

#130173

Purpose

PPI (BiFC/TriFC) assay vector

Depositor

Gary Lewin

Insert

GFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pRDAX-489 GFP[1] L3 Stuffer/Intein1 L4 GFP[2] IRES mCherry GFP(1-9)

Plasmid

#130172

Purpose

PPI (BiFC/TriFC) assay vector

Depositor

Gary Lewin

Insert

GFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pRDAX-488 GFP[1] L6 Stuffer/Intein4 L7 GFP[2] IRES mCherry GFP(1-9)

Plasmid

#130171

Purpose

PPI (BiFC/TriFC) assay vector

Depositor

Gary Lewin

Insert

GFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pRDAX-487 GFP[1] L6 Stuffer/Intein3 L7 GFP[2] IRES mCherry GFP(1-9)

Plasmid

#130170

Purpose

PPI (BiFC/TriFC) assay vector

Depositor

Gary Lewin

Insert

GFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pRDAX-486 GFP[1] L6 Stuffer/Intein2 L7 GFP[2] IRES mCherry GFP(1-9)

Plasmid

#130169

Purpose

PPI (BiFC/TriFC) assay vector

Depositor

Gary Lewin

Insert

GFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pRDAX-485 GFP[1] L6 Stuffer/Intein1 L7 GFP[2] IRES mCherry GFP(1-9)

Plasmid

#130168

Purpose

PPI (BiFC/TriFC) assay vector

Depositor

Gary Lewin

Insert

GFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pRDAX-492 GFP[1] L3 Stuffer/Intein4 L4 GFP[2] IRES mCherry GFP(1-9)

Plasmid

#130175

Purpose

PPI (BiFC/TriFC) assay vector

Depositor

Gary Lewin

Insert

GFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 9, 2020

Availability

Academic Institutions and Nonprofits only

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piggyBac-rtTA (4th_Gen)-SNCA(A53T)-sfGFP-IRES-NGN2-puro (VK_1123)

Plasmid

#209080

Purpose

Ngn2 mediated cortical neuron induction, along with SNCA(A53T)-sfGFP over-expression

Depositor

Vikram Khurana

Article

Lam et al bioRxiv 2022.11.08.515615

Inserts

SNCA (SNCA Human)
NGN2 (Neurog2 Mouse)

Use

Tags

sfGFP

Expression

Mammalian

Mutation

A53T

Promoter

TRE4G

Available since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pK038.CAG-loxP-stop-loxP-EGFP-ires-tTA-WPRE (Supernova)

Plasmid

#85006

Purpose

For Cre/loxP-based Supernova-GFP, pK038 should be used with pK031. The GFP fragment of pK038 can be replaced with another gene by using SalI and EcoRV sites.

Depositor

Takuji Iwasato

Article

Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

Insert

EGFP-ires-tTA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pK029.CAG-loxP-stop-loxP-RFP-ires-tTA-WPRE (Supernova)

Plasmid

#69138

Purpose

For the Supernova, a series of vector systems for single-cell labeling and gene function sudies in vivo

Depositor

Takuji Iwasato

Article

Mizuno et al Neuron. 2014 Apr 16;82(2):365-79. doi: 1

Insert

CAG-loxP-stop-loxP-RFP-ires-tTA-WPRE

Use

Tags

none

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pLL3.7m-rsGreenF-E-Geminin(1-110)-2A-Dronpa-Cdt-IRES-H1-Skylan62A-2A-YFP-SLBP

Plasmid

#205762

Purpose

Single-color FUCCI4 senor for transitions of all four cell cylce phases

Depositor

Edward Boyden

Article

Qian et al Cell. 2023 Dec 7;186(25):5656-5672.e21.

Insert

rsGFE-Geminin(1-110)-2A-Dronpa-Cdt-IRES-H1-SK62A-2A-YFP-SLBP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

wt

Promoter

Available since

Oct. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pK068.CAG-FRT-stop-FRT-EGFP-ires-tTA-WPRE (Supernova)

Plasmid

#85008

Purpose

For Flpe/FRT-based Supernova-GFP, pK068 should be used with pK036. The GFP fragment of pK068 can be replaced with another gene by SalI and EcoRV.

Depositor

Takuji Iwasato

Article

Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

Insert

EGFP-ires-tTA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pPB-Ins-TRE3Gp-KRAB-dCas9-ecDHFR-IRES-GFP-EF1Ap-Puro

Plasmid

#183410

Purpose

piggyBAC-based doxycycline- and trimethoprim-inducible KRAB-dCas9 CRISPR interference plasmid for enhancer and promoter repression in mammalian cells

Depositor

Azim Surani

Article

Tang et al Nat Cell Biol. 2022 Apr;24(4):448-460. d

Insert

KRAB-dCas9-ecDHFR

Use

CRISPR and Synthetic Biology; Piggybac

Tags

Expression

Mammalian

Mutation

Promoter

TRE3G

Available since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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pK037.CAG-FRT-stop-FRT-RFP-ires-tTA-WPRE (Supernova)

Plasmid

#85038

Purpose

For Flpe/FRT-based Supernova-RFP, pK037 should be used with pK036.

Depositor

Takuji Iwasato

Article

Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

Insert

FRT-stop-FRT

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pLV-pGK-Nluc-Fluc-NLS-P2A-H2B-mCherry-IRES-Hygro

Plasmid

#115684

Purpose

NanoLuc-Firefly Luciferase fusion with constitutive nuclear expression for calibration of Nanoluc signal to Firefly Luciferase signal. H2B-mCherry marks chromatin with red fluorescence.

Depositor

David Suter

Article

Mandic et al Methods. 2017 May 1;120:65-75. doi: 10.1

Insert

Nluc-Fluc-NLS-P2A-H2B-mCherry-IRES-Hygro

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV hSyn mCh-IRES-SspB(milli)-BoNT/B(147-441, Y365A)

Plasmid

#122985

Purpose

AAV plasmid with human synapsin promoter driving mCh and SspB(milli)-BoNT/B amino acids 147-441 separated with an IRES element. Co-express with BoNT/B(1-146)-iLID construct for PA-BoNT

Depositor

Matthew Kennedy

Article

Liu et al Neuron. 2019 Jan 15. pii: S0896-6273(19)

Insert

mCh-IRES-SspB(milli)-BoNT/B(147-441)

Use

AAV

Tags

mCh

Expression

Mammalian

Mutation

SspB contains A58V,R73Q "milli" mutatio…

Promoter

human synapsin

Available since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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