We narrowed to 17,206 results for: puro

1. pPB-EF1A-Puro-mCherry-RAB7

   Plasmid

   #221555

   Purpose

   Expresse Cherry-tagged human Rab7 in mammalian cells. Compatible with piggybac transposase for genome integration.

   Depositor

   Shawn Ferguson

   Article

   Talaia et al bioRxiv [Preprint]. 2023 Dec 17:2023.12.

   Insert

   Rab7A (RAB7A Human)
   

   Use

   Piggybac

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   July 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDNR-mCherry-T2A-3xPuroR-Frame 1

   Plasmid

   #206992

   Purpose

   Donor plasmid for Frame 1 with mCherry tag and triple Puromycin resistant marker (3xPuroR)

   Depositor

   Alejandro Chavez

   Article

   Kim et al Sci Adv. 2024 May 3;10(18):eadg8771. doi

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   Available since

   April 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDNR-mCherry-T2A-3xPuroR-Frame 2

   Plasmid

   #206993

   Purpose

   Donor plasmid for Frame 2 with mCherry tag and triple Puromycin resistant marker (3xPuroR)

   Depositor

   Alejandro Chavez

   Article

   Kim et al Sci Adv. 2024 May 3;10(18):eadg8771. doi

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   Available since

   April 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSLQ5465_pHR_hU6-crScaffold_EF1a-PuroR-T2A-BFP

   Plasmid

   #155307

   Purpose

   Rfx Cas13d guide cloning lentiviral vector with constitutively expressed puromycin resistance 2A-tagged with tagBFP

   Depositor

   Stanley Qi

   Article

   Abbott et al Cell. 2020 May 14;181(4):865-876.e12. do

   Insert

   Rfx Cas13d CRISPR RNA puromycin resistance 2A-tagged BFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6, EF1a

   Available since

   July 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLV-MPAct-mRuby3-IRES-Puro

   Plasmid

   #155231

   Purpose

   lentiviral MPAct construct

   Depositor

   Tobias Meyer

   Article

   Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

   Insert

   Lentiviral expression of MPAct-mRuby sensor
   

   Use

   Lentiviral

   Tags

   mRuby3

   Expression

   Mutation

   Promoter

   Available since

   Aug. 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. GG-EBNA-EEA-5guides-PGK-Puro

   Plasmid

   #102898

   Purpose

   EBNA episome plasmid for U6 promoter-driven expression of 5 gRNAs targeting EEA-motif. Includes PGK-puro selection cassette.

   Depositor

   Timo Otonkoski

   Article

   Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

   Insert

   EEA-5guides-PGK-Puro
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. Lv EF1a SS18-Frb PGK Puro

   Plasmid

   #102811

   Purpose

   Lentiviral plasmid from FIRE-Cas9 system to express SS18-Frb fusion protein

   Depositor

   Jerry Crabtree

   Article

   Braun et al Nat Commun. 2017 Sep 15;8(1):560. doi: 1

   Insert

   SS18-Frb
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Dec. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti CMVtight Puro DEST (w768-1)

   Plasmid

   #26430

   Depositor

   Eric Campeau

   Type

   Empty backbone

   Use

   Lentiviral; Tet-on advanced destination vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 17, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMSCV(gfp)U6sgRNA5(BbsI)-PGKpuroBFP

   Plasmid

   #102798

   Purpose

   Retrovirus for delivery of one sgRNA (self-targeting) - GFP-targeting control plasmid contains GFP instead of PuroR

   Depositor

   Sarah Teichmann

   Article

   Henriksson et al Cell. 2019 Jan 7. pii: S0092-8674(18)315

   Inserts

   sgRNA
   EGFP
   

   Use

   CRISPR and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   H231L

   Promoter

   Available since

   Aug. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti CMVtight eGFP Puro (w771-1)

    Plasmid

    #26431

    Purpose

    2nd generation lentiviral vector

    Depositor

    Eric Campeau

    Insert

    Enhanced Green Fluorescent Protein
    

    Use

    Lentiviral; Tet-on advanced vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-SlugABE-NNG-P2A-puro

    Plasmid

    #214354

    Purpose

    Expresses SlugABE-NNG and puromycin resistance genes

    Depositor

    Yongming Wang

    Article

    Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

    Insert

    SlugABE-NNG and puromycin resistance genes
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Q782R/S888R/L906R/N984S/E1012K/K1016I

    Promoter

    Available since

    Feb. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. TRIPZ-CTID195-GSQ-SUMO1nc-PURO

    Plasmid

    #208035

    Purpose

    Enables inducible expression of C-terminal Split-TurboID-fused SUMO1nc, to perform SUMO-ID; nc = non-cleavable mutation; selection with puromycin

    Depositor

    James Sutherland, Rosa Barrio

    Article

    Barroso-Gomila et al Nat Commun. 2021 Nov 18;12(1):6671. doi:

    Insert

    SUMO1nc (SUMO1 Human)
    

    Use

    Lentiviral

    Tags

    Myc, C-terminal Split-TurboID

    Expression

    Mutation

    Q94P Mutation near C-terminus suppresses cleavage…

    Promoter

    tetO/UBC

    Available since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. TRIPZ-CTID195-GSQ-UBnc-PURO

    Plasmid

    #208037

    Purpose

    Enables inducible expression of C-terminal Split-TurboID-fused UBnc, to perform Ubiquitin-ID; nc = non-cleavable mutation; selection with puromycin

    Depositor

    James Sutherland, Rosa Barrio

    Article

    Barroso-Gomila et al Nat Commun. 2021 Nov 18;12(1):6671. doi:

    Insert

    Ubnc (UBC Human)
    

    Use

    Lentiviral

    Tags

    Myc, C-terminal Split-TurboID

    Expression

    Mutation

    L73P Mutation near C-terminus suppresses cleavage…

    Promoter

    tetO/UBC

    Available since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pKLV2-U6gRNASAM(gEmpty)-TREGFP-PGKpuroBFP-W

    Plasmid

    #112926

    Purpose

    CRISPR-a reporter assay lentiviral vector (empty gRNA control)

    Depositor

    Kosuke Yusa

    Article

    Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

    Insert

    hU6-gRNASAM(empty), TREGFP, PGKpuroGFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLV[TetOn]-Puro-TRE3G>mAscl1

    Plasmid

    #198755

    Purpose

    Vector encodes the gene for murine Ascl1 under control of a Tet-controlled promoter and the puromycin resistance gene under control of a constitutive PGK promoter

    Depositor

    Nael Nadif Kasri

    Article

    Mossink et al Mol Psychiatry. 2022 Jan;27(1):1-18. doi

    Insert

    Ascl1 (Ascl1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    TRE3G

    Available since

    July 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. AttB_ACE2_IRES-mCherry-H2A-P2A-PuroR

    Plasmid

    #171594

    Purpose

    Strong human ACE2 abundance from Bxb1 landing pad

    Depositor

    Kenneth Matreyek

    Article

    Shukla et al PLoS Pathog. 2021 Jul 16;17(7):e1009715.

    Inserts

    ACE2 (ACE2 Human)
    mCherry
    H2A
    Pac
    

    Use

    Synthetic Biology; Promoterless bxb1 dna recombin…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEpicVector3mC_Puro-T2A-mCherry-StrepTagII-ProtC-HA

    Plasmid

    #162109

    Purpose

    mCherry linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

    Depositor

    Julien Sage

    Article

    Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

    Insert

    Puromycin resistant; mCherry linked to StrepTagII-ProtC-HA
    

    Use

    Lentiviral

    Tags

    StrepTagII-ProtC-HA

    Expression

    Mutation

    WT

    Promoter

    EF1A

    Available since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMSCV-FLAG-2xSTREP-CDKN1B-Puro

    Plasmid

    #172611

    Purpose

    Retroviral vector for the constitutive expression of FLAG-2xSTREP-tagged p27 and a puromycin resistance marker in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    CDKN1B (CDKN1B Human)
    

    Use

    Retroviral

    Tags

    FLAG-2xSTREP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEpicVector3mC_Puro-T2A-mCherry-ProtC-HA-FLAG

    Plasmid

    #162115

    Purpose

    mCherry linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

    Depositor

    Julien Sage

    Article

    Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

    Insert

    Puromycin resistant; mCherry linked to ProtC-HA-FLAG
    

    Use

    Lentiviral

    Tags

    ProtC-HA-FLAG

    Expression

    Mutation

    WT

    Promoter

    EF1A

    Available since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEpicVector3G_Puro-T2A-EGFP-VSVg-HA-FLAG

    Plasmid

    #162086

    Purpose

    EGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.

    Depositor

    Julien Sage

    Article

    Rovira-Clave et al Cancer Cell. 2022 Nov 14;40(11):1423-143

    Insert

    Puromycin resistant; EGFP linked to VSVg-HA-FLAG
    

    Use

    Lentiviral

    Tags

    VSVg-HA-FLAG

    Expression

    Mutation

    WT

    Promoter

    EF1A

    Available since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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