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pPB_TRE3G_scFV-sfGFP-Ezh2FL-CatMut

Plasmid

#235584

Purpose

Dox-inducible expression of control catalytic-mutant Ezh2 fused with scFV

Depositor

Jamie Hackett

Article

Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

Insert

Ezh2 (Ezh2 Mouse)

Use

CRISPR

Mutation

Y726D

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pHR-PD-1-SNAP

Plasmid

#223576

Purpose

Lentiviral expression of human PD-1 with SNAP tag in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Cell Rep. 2018 Jul 10;24(2):379-390.e6.

Insert

PD-1 (PDCD1 Human)

Use

Lentiviral; mammalian expression

Tags

SNAP

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG_Xph20-mEos3.2-CCR5TC

Plasmid

#135532

Purpose

Encodes a specific PSD-95 binder (Xph20) fused to mEos3.2, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain

Depositor

Matthieu Sainlos

Article

Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

Insert

Xph20

Tags

mEos3.2

Expression

Mammalian

Promoter

CAG

Available Since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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SuperNova/pRSETB

Plasmid

#53234

Purpose

monomeric photosensitizing fluorescent protein for chromophore-assisted light inactivation

Depositor

Takeharu Nagai

Article

Takemoto et al Sci Rep. 2013;3:2629. doi: 10.1038/srep0

Insert

monomeric photosensitizing fluorescent protein for chromophore-assisted light inactivation

Tags

6xHis, T7 tag, and Xpress tag

Expression

Bacterial

Available Since

June 9, 2014

Availability

Academic Institutions and Nonprofits only

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AAV:ITR-U6-sgRNA(backbone)-hSyn-Cre-2A-EGFP-KASH-WPRE-shortPA-ITR

Plasmid

#60231

Purpose

Expresses Cre recombinase and KASH-tagged EGFP from the hSyn promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.

Depositor

Feng Zhang

Article

Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

Inserts

sgRNA
Cre recombinase
EGFP

Use

AAV, CRISPR, Cre/Lox, and Mouse Targeting

Tags

Cre-HA-P2A and EGFP-KASH

Expression

Mammalian

Promoter

U6 and hSyn

Available Since

Oct. 9, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.Flex.GCaMP6s.WPRE.SV40

Plasmid

#100842

Purpose

AAV expression of Cre recombinase-activated ultrasensitive protein calcium sensor from CAG promoter

Depositor

Douglas Kim, GENIE Project

Article

Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

Has Service

AAV1, AAV5, and AAV9

Insert

GCaMP6s

Use

AAV

Expression

Mammalian

Mutation

GCaMP3-K78H T302L R303P D380Y T381R S383T R392G

Promoter

CAG

Available Since

Feb. 28, 2018

Availability

Academic Institutions and Nonprofits only

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SSB-wt

Plasmid

#197260

Purpose

wild type E. coli SSB in pET21 expresses in BL21 under T7 control with lactose-amp resistance

Depositor

Timothy Lohman

Article

Antony et al J Mol Biol. 2013 Nov 29;425(23):4802-19.

Insert

wt E. coli SSB

Tags

none

Expression

Bacterial

Promoter

T7

Available Since

March 22, 2023

Availability

Academic Institutions and Nonprofits only

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pAW019-2

Plasmid

#85614

Purpose

Inducible dCas9 integration vector for Bacillus subtilis

Depositor

Perry Chou

Article

Westbrook et al Appl Environ Microbiol. 2016 Jun 3. pii:

Insert

dcas9

Expression

Bacterial

Promoter

PxylA (B. megaterium)

Available Since

Jan. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pLX304-EV-IRES-GFP

Plasmid

#187640

Purpose

Empty Vector control modified to express GFP

Depositor

William Kaelin

Article

Nicholson et al Sci Signal. 2019 Oct 1;12(601). pii: 12/

Insert

EV

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pBAD-LacI

Plasmid

#46394

Purpose

Overproduction of LacI

Depositor

Florian Hollfelder

Article

Gatti-Lafranconi et al Microb Cell Fact. 2013 Jul 8;12:67. doi:

Insert

LacI

Tags

6xHIS tag

Expression

Bacterial

Mutation

silent T to C mutation at position 19

Promoter

arabinose promoter

Available Since

Aug. 12, 2013

Availability

Academic Institutions and Nonprofits only

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