We narrowed to 18,833 results for: IRE

1. PBRPyCAG-LoxP-ERAS-STOPcassette-Loxp-DsRed-IRES-PURO

   Plasmid

   #52419

   Purpose

   Constitutive mammalian expression of ERAS. Upon CRE mediated deletion of ERAS insert, dsRED expression markers is truned on due to concomittant removal of stop cassette.

   Depositor

   Jacob Hanna

   Article

   Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

   Insert

   ERAS (ERAS Human)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   CAGGS

   Available since

   Sept. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. tetO-MyoD-T2A-GFPnls-mPGK-rtTA-IRES-Puro

   Plasmid

   #69546

   Purpose

   Tet-On system expressing MyoD-2A-GFP-2xNLS.

   Depositor

   Charles Gersbach

   Inserts

   eGFP
   Myod1 (Myod1 Mouse)
   

   Use

   Lentiviral

   Tags

   2x nuclear localization sequence and T2A "sk…

   Expression

   Mutation

   Removed stop codon to fuse it to the 2A-eGFPnls i…

   Promoter

   Available since

   April 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHAGE-IRES-puro-NLS-NLS-dHgm4Cas13b-3xGFP-NLS

   Plasmid

   #191368

   Purpose

   Overexpression of NLS-NLS-dHgm4Cas13b-3xGFP-NLS in human cells

   Depositor

   Ling-Ling Chen

   Article

   Yang et al Cell Insight. 2022 Jun 15;1(4):100044. d

   Insert

   NLS-NLS-dHgm4Cas13b-3xGFP-NLS
   

   Use

   Tags

   3xGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHAGE-IRES-puro-NLS-dAdmCas13d-EGFP-NLS-3xFlag

   Plasmid

   #132412

   Purpose

   overexpression in human cells

   Depositor

   Ling-Ling Chen

   Article

   Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

   Insert

   dAdmCas13b
   

   Use

   Lentiviral

   Tags

   flag

   Expression

   Mammalian

   Mutation

   R269A; H274A; R837A; H842A

   Promoter

   Available since

   April 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMIR-Dsred-IRES-His-Halo-Tev-Keap C288S

   Plasmid

   #62459

   Purpose

   expresses his-tagged C288S human Keap1 protein

   Depositor

   Yimon Aye

   Article

   Parvez et al J Am Chem Soc. 2014 Dec 31.

   Insert

   Keap1 (KEAP1 Human)
   

   Use

   Tags

   His-Halo-Tev

   Expression

   Mammalian

   Mutation

   Changed Cysteine 288 of Keap1 to serine

   Promoter

   CMV

   Available since

   March 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. NLS-GI-VP16-IRES-NLS-GAL4DBD-FKBP-SMBiT-FKF1

   Plasmid

   #183402

   Purpose

   Dual expression of GI-VP16 and GAL4DBD-FKBP-SmBiT-FKF1 in the nucleus

   Depositor

   Alice Ting

   Article

   Erdenee et al ACS Synth Biol. 2022 Mar 18;11(3):1086-1

   Insert

   GI-VP16-IRES-GAL4DBD-FKBP-SMBiT-FKF1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. NLS-GI-VP64-IRES-NLS-GAL4DBD-M13-SmBiT-FKF1

   Plasmid

   #183400

   Purpose

   Dual expression of GI-VP64 and GAL4DBD-M13-SmBiT-FKF1 in the nucleus

   Depositor

   Alice Ting

   Article

   Erdenee et al ACS Synth Biol. 2022 Mar 18;11(3):1086-1

   Insert

   GI-VP64-IRES-GAL4DBD-M13-SmBiT-FKF1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. AttB_[Kozak-mut]ACE2_IRES-mCherry-H2A-P2A-PuroR

   Plasmid

   #171595

   Purpose

   Weak human ACE2 abundance from Bxb1 landing pad

   Depositor

   Kenneth Matreyek

   Article

   Shukla et al PLoS Pathog. 2021 Jul 16;17(7):e1009715.

   Inserts

   ACE2 (ACE2 Human)
   mCherry
   H2A
   Pac
   

   Use

   Synthetic Biology; Promoterless bxb1 dna recombin…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pH2B-CyPet-FHA2-AuroraB Substrate-YPet-IRES-puro2b

   Plasmid

   #40571

   Depositor

   Daniel Gerlich

   Article

   Wurzenberger et al J Cell Biol. 2012 Jul 23;198(2):173-83.

   Insert

   Aurora B FRET-sensor N-terminally fused to histone 2B
   

   Use

   Tags

   CyPet and YPet

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 29, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHAGE-IRES-puro-NLS-dRanCas13b-EGFP-NLS-3xFlag

    Plasmid

    #132409

    Purpose

    overexpression in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

    Insert

    dRanCas13b
    

    Use

    Lentiviral

    Tags

    flag

    Expression

    Mammalian

    Mutation

    H147A; H1044A

    Promoter

    Available since

    Jan. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSLQ2804 pHR: U6-SpsgTRE3G CMV-PYL1-VPR-IRES-mCherry

    Plasmid

    #84258

    Purpose

    Expresses Sp sgTRE3G gRNA with ABA-inducible VPR and mCherry for OR gate

    Depositor

    Stanley Qi

    Article

    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

    Inserts

    Sp sgTRE3G
    PYL1-VPR
    

    Use

    CRISPR and Lentiviral

    Tags

    IRES-mCherry and PYL1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and mouse U6

    Available since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pT2K-CAGGS-U6-stuffer-dCAs9-VP160-T2A-IRES-mCherry

    Plasmid

    #114726

    Purpose

    dCas9-VP160 expression in mammalian cells

    Depositor

    Martine Roussel

    Article

    Vo et al Sci Rep. 2018 Jun 7;8(1):8733. doi: 10.1

    Insert

    dCas9-VP160
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSLQ2827 pHR: U6-SpsgSV40 CMV-PYL1-KRAB-IRES-mCherry

    Plasmid

    #84260

    Purpose

    Expresses Sp sgSV40 gRNA with ABA-inducible KRAB and mCherry for diametric regulation

    Depositor

    Stanley Qi

    Article

    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

    Inserts

    Sp sgSV40
    PYL1-KRAB
    

    Use

    CRISPR and Lentiviral

    Tags

    IRES-mCherry and PYL1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and mouse U6

    Available since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. LV-TRE-MyoD-Stop-LacZ PGK-rtTA-IRES-Puro

    Plasmid

    #61441

    Purpose

    Dox-inducible MyoD with mRNA tag for smFISH

    Depositor

    Charles Gersbach

    Article

    Gibson et al Integr Biol (Camb). 2015 Jun 8;7(6):663-

    Insert

    MyoD (Myod1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. MSCV-TRE-dsRed-miR30_shBrd9_1116-PGK-Venus-IRES-NeoR

    Plasmid

    #75136

    Purpose

    Retroviral expression plasmid encoding inducible shBrd9_1116 (targeting murine Brd9)

    Depositor

    Christopher Vakoc

    Article

    Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

    Insert

    shBrd9_1116
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. MSCV-TRE-dsRed-miR30_shBrd9_1061-PGK-Venus-IRES-NeoR

    Plasmid

    #75135

    Purpose

    Retroviral expression plasmid encoding inducible shBrd9_1061 (targeting murine Brd9)

    Depositor

    Christopher Vakoc

    Article

    Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/

    Insert

    shBrd9_1061
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pPW3761 : CMVd3-HsIRE1(WLLI-GSGS)[359-362]-HaloTag

    Plasmid

    #185683

    Purpose

    Low-level transient expression of IF2 mutant HsIRE1 with a C-terminal HaloTag.

    Depositor

    Peter Walter

    Article

    Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

    Insert

    ERN1 (ERN1 Human, Synthetic)
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    (WLLI-GSGS)[359-362]

    Promoter

    Available since

    March 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHR_UCOE-SFFV-dCas9-XTEN80-KRAB(Kox1)-IRES-mCherry

    Plasmid

    #188770

    Purpose

    dCas9 with a C-term HA-2xNLS-XTEN80(linker)-KRAB(Kox1)

    Depositor

    Jonathan Weissman, Marco Jost

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    dCas9-XTEN80-KRAB(Kox1)
    

    Use

    Lentiviral

    Tags

    HA-2xNLS-XTEN80(linker)-KRAB(Kox1)

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCru5-ACC/ACC/ACC/ACC-mEGFP-IRES-mCherry

    Plasmid

    #49218

    Purpose

    Retroviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.

    Depositor

    Clifford Wang

    Article

    Ferreira et al Proc Natl Acad Sci U S A. 2013 Jul 9;110

    Inserts

    mEGFP
    mCherry
    

    Use

    Retroviral and Synthetic Biology

    Tags

    SfuI site to create C-terminal fusions

    Expression

    Mammalian

    Mutation

    Promoter

    Viral LTR (or CMV)

    Available since

    Dec. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHAGE-IRES-puro-NLS-HyperdFnCas12a-EGFP-NLS-3xFlag

    Plasmid

    #236376

    Purpose

    Overexpression of HyperdFnCas12a in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Nat Methods. 2024 Sep;21(9):1646-1657. d

    Insert

    HyperdFnCas12a
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    E184R, T279R, D340R, D409R

    Promoter

    Available since

    July 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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