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We narrowed to 6,447 results for: nls

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Showing: 2321 - 2340 of 6447 results

  5. pAAV_hSyn_HA-TALE(HD)-Tet1S-NLS_2A_EGFP_W3SL

    Plasmid
    #172200
    Purpose
    AAV sequence-specific control vector for TALE-Tet1S-SID4X. Synapsin promoter for neuronal expression. C-terminal, self-cleaving EGFP marker.
    Depositor
    Garrett Kaas
    Article
    Greer et al J Neurosci. 2021 Jan 27;41(4):578-593. d
    Insert
    HA-TALE(HD)-Tet1S-NLS_2A_EGFP_W3SL
    Use
    AAV and Mouse Targeting; Tale
    Tags
    HA, NLS, and T2A-EGFP
    Expression
    Mammalian
    Mutation
    transcription regulatory domain removed
    Promoter
    hSyn human synapsin (mammalian neuronal expressio…
    Available Since
    Aug. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    172200-plasmid-map-sequence-id-338847

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  6. pRN3P-NLS-EGFP-deadBtgh-3NLS

    Plasmid
    #194470
    Purpose
    Encodes for catalytically inactive bacterial O-GlcNAcase BtGH84 fused to enhanced GFP and NLSs for effective nuclear localization, in backbone for T3 In Vitro Transcription.
    Depositor
    Mathieu Boulard
    Article
    Formichetti et al Dev Cell 10 Jan 2024
    Insert
    BtGH84
    Use
    For in vitro transcription from t3 promoter after…
    Tags
    SV40 Nuclear Localization Signal, SV40 Nuclear Lo…
    Mutation
    Aspartic Acid 242 (in Dennis et al.) changed to A…
    Available Since
    Jan. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    194470-plasmid-map-sequence-id-384877

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  7. pCMV-T7-nSpCas9(H840A)-BPNLS(SV40)-3xFLAG-P2A-EGFP (HES140)

    Plasmid
    #185490
    Purpose
    CMV and T7 promoter expression plasmid for human codon usage nSpCas9(H840A) with a C-terminal bi-partite NLS, 3x FLAG, and P2A-eGFP
    Depositor
    Benjamin Kleinstiver, Raul Perez-Jimenez
    Article
    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:
    Insert
    human codon usage nSpCas9(H840A)-BPNLS-P2A-eGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-eGFP
    Expression
    Mammalian
    Mutation
    H840A
    Promoter
    CMV and T7
    Available Since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    185490-plasmid-map-sequence-id-362676

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  8. pCMV-SV40NLS-Stab-exin21-Bxb1(A315R)-Stab (CJT604)

    Plasmid
    #248210
    Purpose
    SV40 NLS-, stabilon-, and exin21-tagged Bxb1 (A315R) recombinase, expressed from a CMV promoter.
    Depositor
    Benjamin Kleinstiver
    Article
    Tou et al Nature. 2026 Mar 11. doi: 10.1038/s41586
    Insert
    SV40NLS-Stab-exin21-Bxb1(A315R)-Stab
    Use
    CRISPR; In vitro transcription
    Tags
    SV40NLS
    Expression
    Mammalian
    Mutation
    Bxb1(A315R)
    Promoter
    CMV and T7
    Available Since
    April 15, 2026
    Availability
    Academic Institutions and Nonprofits only
    248210-plasmid-map-sequence-id-506606

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  9. NLS-R-NmeCas9 (D16A)-HA-NLS_sgRNA EZH guide

    Plasmid
    #246438
    Purpose
    All-in-one plasmid. Expresses R-NmeCas9 in mammalian cells for RNA knockdown. Spacer targeting EZH2 gene.
    Depositor
    Ailong Ke
    Article
    Xu et al Cell. 2025 Aug 18:S0092-8674(25)00854-2.
    Inserts
    NmeCas9
    Nme-sgRNA
    Tags
    HA tag, NLS, and SV40 NLS
    Expression
    Mammalian
    Mutation
    changed Aspartic acid 16 to Alanine, deleted amin…
    Promoter
    EF-1 alpha and U6
    Available Since
    Oct. 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    246438-plasmid-map-sequence-id-490930

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  16. pCAG-hAsCas12a-RVR(S542R/K548V/N552R)-NLS(nucleoplasmin)-3xHA (AAS1065)

    Plasmid
    #114092
    Purpose
    CAG promoter expression plasmid for human codon optimized AsCas12a-RVR(S542R/K548V/N552R) nuclease with C-terminal NLS and HA tag
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized AsCas12a (S542R/K548V/N552R)
    Tags
    NLS(nucleoplasmin) and 3xHA
    Expression
    Mammalian
    Mutation
    S542R, K548V and N552R
    Available Since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114092-plasmid-map-sequence-id-230539

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  18. TV_EMPTY-ires-mNeon-nls-p2a-dtr-wpre_pgk-puro

    Plasmid
    #251174
    Purpose
    Empty targeting vector (SapI sites intact) for C-terminal knockin at desired locus to enable reporting of expression by mNeonGreen and depletion of positive cells with DTR
    Depositor
    Hugo Snippert
    Insert
    double SapI flanked ires-mNeon-nls-p2a-dtr-wpre_pgk-puro
    Use
    CRISPR
    Tags
    ires-mNeon-nls-p2a-dtr
    Available Since
    Feb. 20, 2026
    Availability
    Academic Institutions and Nonprofits only
    251174-plasmid-map-sequence-id-500685

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  20. pL6puro-JeT-pmRevErbA-mClover3-NLS-Pest

    Plasmid
    #240109
    Purpose
    A lentiviral (yellow-green) fluorescent reporter of circadian rhythm, based on the murine Nr1d1-gene (REVERBa protein), expression enhanced by synthtic JeT promoter.
    Depositor
    Achim Kramer
    Article
    Gabriel et al bioRxiv 2025.06.30.661926
    Insert
    murine Nr1d1 promoter+mClover3-NLS-Pest-Reporter (Nr1d1 Mouse)
    Use
    Lentiviral
    Mutation
    added JeT Promoter
    Available Since
    Aug. 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    240109-plasmid-map-sequence-id-481752

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Showing: 2321 - 2340 of 6447 results