We narrowed to 18,704 results for: Met;
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Plasmid#65624PurposeFluorescent reporter to monitor the activty of Bxb1; If Bxb1 and attB donor plasmid containing GFP is present in the cell, recombination of the donor into the reporter leads to expression of GFP.DepositorInsertattP
UseBxb1ExpressionMammalianAvailable SinceAug. 31, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET23a-His-hRanGAP1tail
Plasmid#53139PurposeE.coli expression of Cterminal domain of human RanGAP1 with Nterminal His-tagDepositorInsertRanGAP1 (RANGAP1 Human)
TagsHisExpressionBacterialMutationC-terminal fragment (amino acids 398-587)PromoterT7Available SinceMay 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
CIBN-GFP-TMD
Plasmid#79571Purposeexpression of CIBN domain fused to GFP tagged mouse Syntaxin1 TMD domain (transmembrane domain and polybasic region)DepositorInsertsyntaxin 1A (Stx1a Mouse)
TagsCIBN and EGFPExpressionMammalianMutationaa 249_288 (C-terminal region (polybasic stretch …PromoterCMVAvailable SinceJuly 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-CBX1mut-PA-mCherry
Plasmid#138251PurposeTriple-mutant CBX1 chromodomain (residues 20-73) tagged with photoactivatable mCherry (PAmCherry). Mutations V3E/K6E/D40S correspond to positions V22/K25/D59 in full-length CBX1.DepositorInsertCBX1mut-PAmCherry
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianPromoterCMVAvailable SinceJuly 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-RIPK3[V458A][Q459A][V460A][G461A]-FLAG
Plasmid#199328Purposemutated RHIM motive (interaction inhibited)DepositorInsertReceptor-interacting serine/threonine-protein kinase 3 (RIPK3 Human)
TagsFLAGExpressionMammalianMutationchanged V458, Q459,V460 and G461 to alanineAvailable SinceJune 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hSyn_SpikeyGi2_kv2.1
Plasmid#195313PurposeGenetically-encoded voltage indicator SpikeyGi2, soma and AIS targeted, in AAV vector under the neuronal-specific promoter hSynDepositorInsertSpikeyGi2
UseAAVPromoterhSynAvailable SinceMarch 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV hSyn hPGK1-mRuby
Plasmid#220911PurposeNeuronal specific expression of the fusion construct PGK1-mRubyDepositorAvailable SinceJune 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
TIMP1-bio-His
Plasmid#52089PurposeExpresses full-length Metalloproteinase inhibitor 1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertTIMP1 (TIMP1 Human)
TagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianPromoterCMVAvailable SinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
CRY2-OCRL(D523G)
Plasmid#79567Purposeexpression of CRY2PHR fused to phosphatase dead mutant of OCRLDepositorInsertOCRL (OCRL Human)
TagsCRY2 (photolyase homology region domain of Arabid…ExpressionMammalianMutationaa 234_539 and phosphatase mutation, D523GPromoterCMVAvailable SinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE-mCherry-mTrim21ΔRING-Box
Plasmid#105523PurposeT7 promotor drives in vitro transcription of mCherry-tagged mouse TRIM21 with deleted RING-box mRNADepositorInsertTRIM21 (Trim21 Mouse)
UseIn vitro transcriptionTagsmCherryMutationdeletion of RING domain (aa124-462)PromoterT7Available SinceMarch 7, 2018AvailabilityAcademic Institutions and Nonprofits only