174,739 results
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Plasmid#83917PurposeEncodes an E. coli codon optimized version of dAChE4 - a designed variant of human acetylcholinesterase bearing 51 mutations with ~1800 fold higher functional expression levels in E. coliDepositorInsertDesigned hAChE, codon optimised for E.coli expression (ACHE Human, Synthetic)
TagsHis, S-tag, and TRXExpressionBacterialMutationV13T,K24T,M43V,L49R,V61W,S68N,E92N,T110K,S111N,T1…PromoterT7 promoterAvailable SinceMarch 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-mTubb3
Plasmid#87116PurposeAAV backbone plasmid including GFP knock-in donor and Tubb3gRNA for HITIDepositorInsertU6-mTubb3sgRNA-GFP-EF1a-mCherryKASH-hGHpA
UseAAV and CRISPRAvailable SinceMarch 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_V5-APEX2-Sec61B
Plasmid#83412PurposeExpressed N-term tagged APEX2 on Sec61B in mammalian cellDepositorInsertV5-APEX2-Sec61B (SEC61B Human, Synthetic)
TagsAPEX2 and V5ExpressionMammalianPromoterCMV-FAvailable SinceMarch 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGL4.0-TERT G250A
Plasmid#84925Purposeluciferase reporter for mutant TERT promoterDepositorInsertTERT promoter (TERT Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationG250APromoterTERTAvailable SinceMarch 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2 GFP-Rtn4a
Plasmid#86684PurposeLentivirus to stably express fluorescent human protein in mammalian cellsDepositorAvailable SinceFeb. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 - BirA* (AA141-321) - HA - NIPP1
Plasmid#86885PurposeC-terminal part of the split-BioIDDepositorInsertBirA* (AA141-321)- HA- NIPP1]
TagsHA tagExpressionMammalianMutationBirA Amino Acids 141-321Available SinceFeb. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
T-2A-EGFP-PGK-Puro
Plasmid#83344PurposeDonor vector to knocin EGFP into human Brachyury(T) locusDepositorInsertHuman Brachyury knockin homology arms (TBXT Human)
Tags2A-EGFPAvailable SinceFeb. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBA407
Plasmid#85970PurposeUPRE-mCh-Ubc-NeoDepositorInsertsmCherry
Neo
UseLentiviralExpressionMammalianAvailable SinceFeb. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSB1A3 EcoRI-RTX with EcoRI Methylase-AmilCP
Plasmid#85165PurposeProvides the coding region to express the EcoRI restriction enzyme. The RTX tag allows the EcoRI enzyme to be isolated via calcium-precipitation.DepositorInsertEcoRI-RTX and EcoRI Methylase-AmilCP coding sequences
TagsAmilCP and RTXExpressionBacterialPromoterJ23100 promoterAvailable SinceDec. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
Dazl-CPG-TV
Plasmid#70144Purposea targeting vector, to target the endogenous DAZL CPG island with Snrp-GFP-polyADepositorInsert5'hDAZL-snrp-GFP-polyA-pgK-PURO-3'hDAZL
UseMouse Targeting and Synthetic BiologyAvailable SinceDec. 16, 2016AvailabilityAcademic Institutions and Nonprofits only