161,946 results

1. pKM-U6-reRNA-acceptor-2xBbsI

   Plasmid

   #205633

   Purpose

   Cloning and U6-drive expression of ISDra2 TnpB reRNAs

   Depositor

   Gerald Schwank

   Article

   Marquart et al Nat Methods. 2024 Sep 23. doi: 10.1038/s

   Insert

   ISDra2-TnpB reRNA with placeholder for guide cloning with BbsI
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Sept. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. PB-TO-hNIL

   Plasmid

   #172113

   Purpose

   Piggybac Tet-ON plasmid for differentiating hiPSCs into lower motor neurons via NGN2, ISL1, and LHX3 expression

   Depositor

   iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

   Article

   Pantazis et al Cell Stem Cell. 2022 Dec 1;29(12):1685-1

   Inserts

   hNGN2 (NEUROG2 Human)
   hISL1 (ISL1 Human)
   hLHX3 (LHX3 Human)
   puroR-T2A-mycNLS-mTagBFP2
   

   Use

   Tags

   T2A-mycNLS-mTagBFP2

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a and TRE3G

   Available since

   April 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRDB_270

   Plasmid

   #231111

   Purpose

   C>T base editor

   Depositor

   John Doench, David Root

   Insert

   TadA-CDd-nCas9-SpG-UGI-HA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   D10A

   Promoter

   Available since

   Jan. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. CCR2-DuET

   Plasmid

   #213198

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   CCR2 (CCR2 Human)
   

   Use

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, T7

   Available since

   May 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. mCherry-PIS

   Plasmid

   #119078

   Purpose

   Visualize localization of ER enzyme Phosphatidylinositol Synthase (PIS)

   Depositor

   Gia Voeltz

   Article

   English et al Nat Cell Biol. 2013 Feb;15(2):169-78. do

   Insert

   PIS (CDIPT Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. SaKKH-ABE8e

   Plasmid

   #138502

   Purpose

   A-to-G base editor

   Depositor

   David Liu

   Article

   Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

   Insert

   ecTadA(8e)-nSaCas9 (KKH)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPAGFP-VSVG

   Plasmid

   #11915

   Depositor

   Jennifer Lippincott-Schwartz

   Article

   Presley et al Nature. 1997 Sep 4. 389(6646):81-5.

   Insert

   VSVG
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   (L64F/T65S/V163A/T203H). The PAGFP also contains …

   Promoter

   Available since

   July 3, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.3_SARS2_omicron BA.2

   Plasmid

   #183700

   Purpose

   expressing SARS-CoV-2 BA.2 spike protein for pseudovirus production

   Depositor

   David Nemazee

   Article

   Dacon et al Science. 2022 Jul 12:eabq3773. doi: 10.1

   Insert

   Spike (BA.2) (S SARS-CoV-2)
   

   Use

   Lentiviral and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   c-terminal 18 aa deletion

   Promoter

   CMV

   Available since

   April 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. DP6

   Plasmid

   #140446

   Purpose

   Untargetted bacterial drift

   Depositor

   David Liu

   Article

   Badran et al Nat Commun. 2015 Oct 7;6:8425. doi: 10.1

   Insert

   dnaQ926 dam seqA emrR ugi CDA1 gIII
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCMV-HA-hIFITM3

    Plasmid

    #58397

    Purpose

    Expressed human IFITM3 with an N-terminal HA tag in mammalian cells

    Depositor

    Jacob Yount, Howard Hang

    Article

    Yount et al Nat Chem Biol. 2010 Aug;6(8):610-4. doi:

    Insert

    IFITM3 (IFITM3 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV IE

    Available since

    Sept. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. DsRed-rab11 DN

    Plasmid

    #12680

    Depositor

    Richard Pagano

    Article

    Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

    Insert

    RAB11A (Homo sapiens) (RAB11A Human)
    

    Use

    Tags

    DsRed and HA

    Expression

    Mammalian

    Mutation

    changed serine 25 to asparagine

    Promoter

    Available since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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12. YFC43

    Plasmid

    #89488

    Purpose

    gateway-based Bimolecular Fluorescence Complementation (BiFC) binary vector containing C terminal fragment of YFP

    Depositor

    Alejandro Ferrando

    Article

    Belda-Palazon et al PLoS One. 2012;7(10):e46907. doi: 10.137

    Type

    Empty backbone

    Use

    Tags

    YFP-C (aa 154-240)

    Expression

    Plant

    Mutation

    Promoter

    2x35S

    Available since

    April 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRSF-G1(6/7FTrp)RS

    Plasmid

    #207621

    Purpose

    tRNA synthetase/tRNA pair for the in vivo incorporation of 6-Fluoro-L-Tryptophan (6FTrp) and 7-Fluoro-L-Tryptophan (7FTrp) into proteins in E. coli in response to the amber (TAG) codon

    Depositor

    Thomas Huber

    Article

    Qianzhu et al J Am Chem Soc. 2024 Apr 30. doi: 10.1021

    Insert

    tRNA synthetase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBabe bleo human PPAR gamma 2

    Plasmid

    #11439

    Depositor

    Ronald Kahn

    Insert

    PPAR gamma 2 (PPARG Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 25, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLX304-GW-DCK*-IRES-GFP

    Plasmid

    #176291

    Purpose

    Gateway vector for use in generating POI-DCK* fusion reporter

    Depositor

    William Kaelin

    Article

    Koduri et al Sci Adv. 2021 Feb 5;7(6). pii: 7/6/eabd6

    Insert

    Mutant Deoxycytidine Kinase (DCK*, S74E R104M D133A) (DCK Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    S74E R104M D133A

    Promoter

    CMV

    Available since

    March 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. YFN43

    Plasmid

    #89487

    Purpose

    gateway-based Bimolecular Fluorescence Complementation (BiFC) binary vector containing N terminal fragment of YFP

    Depositor

    Alejandro Ferrando

    Article

    Belda-Palazon et al PLoS One. 2012;7(10):e46907. doi: 10.137

    Type

    Empty backbone

    Use

    Tags

    YFP-N (aa 1-154)

    Expression

    Plant

    Mutation

    Promoter

    2x35S

    Available since

    April 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. PB-tetO-hNIL

    Plasmid

    #197089

    Purpose

    PiggyBac plasmid with tet-inducible expression of transcription factors for motor neuron differentiation

    Depositor

    Brian Wainger

    Article

    Held et al Cell Rep. 2023 Aug 30;42(9):113046. doi:

    Insert

    NGN2-P2A-ISL1-T2A-LHX3 (NEUROG2, ISL1, LHX3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-PE7-P2A-hMLH1dn

    Plasmid

    #222997

    Purpose

    Mammalian expression of SpCas9 PE7 prime editor with P2A-human MLH1dn (codon optimized)

    Depositor

    Brittany Adamson

    Article

    Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

    Insert

    PE7-P2A-hMLH1dn
    

    Use

    Tags

    SV40 bpNLS and c-Myc NLS

    Expression

    Mammalian

    Mutation

    Deletion of residues 754-756 in MLH1 as described…

    Promoter

    CMV

    Available since

    Aug. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAGK62

    Plasmid

    #228122

    Purpose

    Express node 1531 retron with split retron ncRNA and gRNA to edit (10bp barcode) EMX1 gene in human cells

    Depositor

    Seth Shipman

    Article

    Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103

    Insert

    Retron node 1531 split retron ncRNA and gRNA to edit EMX1 gene (10bp barcode) in human cells
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG for RT, U6/H1 for ncRNA/gRNA

    Available since

    Dec. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCIP-AMPKa1_KD

    Plasmid

    #79011

    Purpose

    For mammalian expression of a kinase dead (K47R) version of AMPK alpha1.

    Depositor

    Reuben Shaw

    Article

    Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

    Insert

    AMPK alpha 1 (PRKAA1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    K47R

    Promoter

    pCAGGs

    Available since

    July 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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