We narrowed to 30,834 results for: Eng

1. KAT5 sgRNA2

   Plasmid

   #138188

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human KAT5

   Depositor

   Wen Xue

   Article

   Kwan et al Hepatology. 2019 Jun 12. doi: 10.1002/he

   Insert

   KAT5 (KAT5 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   March 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. lentiCRISPR v2-sgBAP1-1

   Plasmid

   #125837

   Purpose

   KO BAP1 gene

   Depositor

   Boyi Gan

   Article

   Zhang et al Nat Cell Biol. 2018 Oct;20(10):1181-1192

   Insert

   BAP1 (BRCA1 associated protein 1) (BAP1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pTT3 Flrt2-myc

   Plasmid

   #72192

   Purpose

   Express full-length Flrt2with a C-terminal myc tag

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Flrt2 (Flrt2 Mouse)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. lentiCRISPR v2-sgBAP1-2

   Plasmid

   #125838

   Purpose

   KO BAP1 gene

   Depositor

   Boyi Gan

   Article

   Zhang et al Nat Cell Biol. 2018 Oct;20(10):1181-1192

   Insert

   BAP1 (BRCA1 associated protein 1) (BAP1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. PH-Btk-mRuby2

   Plasmid

   #124657

   Purpose

   PI(3,4,5)P3 probe. PH domain of BTK fused with mRuby2

   Depositor

   Cheng-han Yu

   Article

   Zhang et al Mol Biol Cell. 2019 Mar 1;30(5):622-635.

   Insert

   BTK (BTK Human)
   

   Use

   Tags

   mRuby2

   Expression

   Mammalian

   Mutation

   contains only the PH domain of Bruton tyrosine ki…

   Promoter

   Available since

   May 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. miniCRISPRoff-v1

   Plasmid

   #197030

   Purpose

   Vector encoding a human codon-optimized miniCRISPRoff-v1 driven by CBh promoter, guide RNAs compatible with enOsCas12f1 driven by hU6, and mCherry driven by CMV promoter

   Depositor

   Xiangfeng Kong

   Article

   Kong et al Nat Commun. 2023 Apr 11;14(1):2046. doi:

   Insert

   humanized miniCRISRPoff-v1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   D52R / T132R / D228A / D406A

   Promoter

   CBh, CMV, hU6

   Available since

   May 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. dCas12Max-CBE

   Plasmid

   #195337

   Purpose

   vector for encoding a human codon-optimized dCas12Max-CBE driven by CBh promoter, guide RNAs compatible with xCas12i driven by hU6, and mCherry driven by CMV promoter

   Depositor

   Yingsi Zhou

   Article

   Zhang et al Protein Cell. 2023 Jun 28;14(7):538-543.

   Insert

   human codon-optimized dCas12Max-CBE
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   N243R, E336R, D1049A

   Promoter

   CBh, CMV, hU6

   Available since

   Jan. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pHIV-INK4A-mOrange2

   Plasmid

   #110730

   Purpose

   Mammalian Expression of p16INK4A IRES H2B-mOrange2

   Depositor

   Robert Judson-Torres

   Article

   Zeng et al Cancer Cell. 2018 Jul 9;34(1):56-68.e9.

   Insert

   p16INK4A IRES H2B-mOrange2 (CDKN2A Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Cas12Max

   Plasmid

   #195334

   Purpose

   vector for encoding a human codon-optimized Cas12Max driven by CBh promoter, guide RNAs compatible with xCas12i driven by hU6, and mCherry driven by CMV promoter

   Depositor

   Yingsi Zhou

   Article

   Zhang et al Protein Cell. 2023 Jun 28;14(7):538-543.

   Insert

   human codon-optimized Cas12Max
   

   Use

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   N243R

   Promoter

   CBh, CMV, hU6

   Available since

   Jan. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV2-TBG-FLAG-mRIPK1

    Plasmid

    #115341

    Purpose

    Liver-specific adeno-associated viral delivery and mammalian expression of Flag-mRIPK1

    Depositor

    Junying Yuan

    Article

    Geng et al Nat Commun. 2017 Aug 25;8(1):359. doi: 1

    Insert

    RIPK1 (Ripk1 Mouse)
    

    Use

    AAV

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMT-sox10-NLS-BirA-2A-mCherry_Ras

    Plasmid

    #80063

    Purpose

    Sox10 promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
    

    Use

    Zebrafish biotagging

    Tags

    3x HA

    Expression

    Bacterial

    Mutation

    Promoter

    Sox10 promoter

    Available since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pTT3 Flrt1-myc

    Plasmid

    #72191

    Purpose

    Express full-length Flrt1 with a C-terminal myc tag

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Flrt1 (Flrt1 Mouse)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEN35-CDKN2A-Ex2-R

    Plasmid

    #110737

    Purpose

    Encodes Cas9 nickase and gRNA targeting CDKN2A Exon2

    Depositor

    Robert Judson-Torres

    Article

    Zeng et al Cancer Cell. 2018 Jul 9;34(1):56-68.e9.

    Insert

    CDKN2A gRNA (CDKN2A Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. K9L mutant of H3K9me3 biosensor

    Plasmid

    #120807

    Purpose

    FRET biosensor. Eliminating the H3 lysine 9 methylation site in H3K9me3 biosensor to abolish the detection capability

    Depositor

    Yingxiao Wang

    Article

    Peng et al Proc Natl Acad Sci U S A. 2018 Nov 26. p

    Insert

    Truncated YPet-HP1-EV linker-ECFP-mouse histone H3(K9L mutation)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    lysine 9 is mutated to leucine 9 on histone H3

    Promoter

    CMV

    Available since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pEN35-CDKN2A-Ex2-L

    Plasmid

    #110736

    Purpose

    Encodes Cas9 nickase and gRNA targeting CDKN2A Exon2

    Depositor

    Robert Judson-Torres

    Article

    Zeng et al Cancer Cell. 2018 Jul 9;34(1):56-68.e9.

    Insert

    CDKN2A gRNA (CDKN2A Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDB-His-MBP-mGSDMD

    Plasmid

    #123365

    Purpose

    Expression of full-length mouse GSDMD in E. coli

    Depositor

    Hao Wu

    Article

    Orning et al Science. 2018 Nov 30;362(6418):1064-1069

    Insert

    GSDMD (Gsdmd Mouse)
    

    Use

    Tags

    His6-MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDisplay-DNER

    Plasmid

    #51053

    Purpose

    expression of HA-DNER in mammalian cells

    Depositor

    Mineko Kengaku

    Article

    Eiraku et al J Biol Chem. 2002 Jul 12;277(28):25400-7

    Insert

    DNER (Dner Mouse)
    

    Use

    Tags

    HA and signal peptide

    Expression

    Mammalian

    Mutation

    without endogenous signal peptide (1-221bp)

    Promoter

    CMV

    Available since

    Feb. 19, 2014

    Availability

    Academic Institutions and Nonprofits only

18. KAT2A sgRNA2

    Plasmid

    #138186

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human KAT2A

    Depositor

    Wen Xue

    Article

    Kwan et al Hepatology. 2019 Jun 12. doi: 10.1002/he

    Insert

    KAT2A (KAT2A Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. KAT2A sgRNA1

    Plasmid

    #138185

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human KAT2A

    Depositor

    Wen Xue

    Article

    Kwan et al Hepatology. 2019 Jun 12. doi: 10.1002/he

    Insert

    KAT2A (KAT2A Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBabe 3XFLAG-wtGATA6-3XAU1 puro

    Plasmid

    #72607

    Purpose

    Expresses an N-terminally triple FLAG-tagged and C-terminally triple AU1-tagged full length human wtGATA6 in mamalian cells

    Depositor

    Kevin Janes

    Article

    Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:

    Insert

    GATA binding protein 6 (GATA6 Human)
    

    Use

    Retroviral

    Tags

    3XAU1 and 3XFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. C1ORF43-GFP

    Plasmid

    #130879

    Purpose

    Expresses human C1ORF43 with C-terminal GFP tag

    Depositor

    Michael Bassik

    Article

    Jeng et al Cell Host Microbe. 2019 Oct 9;26(4):551-

    Insert

    C1ORF43 (C1orf43 Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pHIV-ARF-mOrange2

    Plasmid

    #110731

    Purpose

    Mammalian Expression of p14ARF IRES H2B-mOrange2

    Depositor

    Robert Judson-Torres

    Article

    Zeng et al Cancer Cell. 2018 Jul 9;34(1):56-68.e9.

    Insert

    p14ARF IRES H2B-mOrange2 (CDKN2A Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. Clone 16 - 3 HK02 pFUSE-IgG-human kappa VL-CH1 hole-clone2

    Plasmid

    #192185

    Purpose

    Clone 16 (HK02) pFUSE-IgG-human kappa VL-CH1 hole-clone2

    Depositor

    Sidi Chen

    Article

    Peng et al Nat Commun. 2022 Mar 28;13(1):1638. doi:

    Insert

    Clone 2 light chain (HK02)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    NA

    Promoter

    Available since

    Oct. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pMTB2-Avi-Cerulean-RanGAP

    Plasmid

    #79878

    Purpose

    Beta-actin2 promoter driving N-term Avi-tagged protein containing Cerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1); flanked by Tol2 sequences

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    Cerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1) (RANGAP1 Chicken)
    

    Use

    Tags

    Avi

    Expression

    Bacterial

    Mutation

    Promoter

    Beta-actin2

    Available since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. GFP-RAB10 K154A

    Plasmid

    #130886

    Purpose

    Expresses human RAB10 K154A mutant with N-terminal GFP tag

    Depositor

    Michael Bassik

    Article

    Jeng et al Cell Host Microbe. 2019 Oct 9;26(4):551-

    Insert

    RAB10 (RAB10 Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    K154A

    Promoter

    Available since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. tdEos-LaminB1-10

    Plasmid

    #57632

    Purpose

    Localization: Nuclear Envelope, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Michael Davidson

    Article

    Shtengel et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

    Insert

    LaminB1 (LMNB1 Human)
    

    Use

    Tags

    tdEos

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 16, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

27. pMT-ubb-Avi-Cerulean-Rpl10a

    Plasmid

    #79882

    Purpose

    Ubiquitin promoter driving Avi-tagged protein containing Cerulean protein fused to zebrafish Rpl10a ribosomal unit (Tryon et al. 2012); flanked by Tol2 sequences

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    Cerulean protein fused to zebrafish Rpl10a ribosomal unit (rpl10a Zebrafish)
    

    Use

    Tags

    Avi

    Expression

    Bacterial

    Mutation

    Promoter

    Ubiquitin

    Available since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLV Myc-LBR

    Plasmid

    #175102

    Purpose

    Lentiviral expression of Myc-tagged mouse Lbr

    Depositor

    Larry Gerace

    Article

    Cheng et al J Proteome Res. 2022 Sep 2;21(9):2197-22

    Insert

    Lbr (Lbr Mouse)
    

    Use

    Lentiviral

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. tdEos-Mito-7

    Plasmid

    #57644

    Purpose

    Localization: Mitochondria, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Michael Davidson

    Article

    Shtengel et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

    Insert

    Mito (COX8A Human)
    

    Use

    Tags

    tdEos

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 5, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

30. tdEos-Golgi-7

    Plasmid

    #57619

    Purpose

    Localization: Golgi Complex, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Michael Davidson

    Article

    Shtengel et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

    Insert

    Golgi (B4GALT1 Human)
    

    Use

    Tags

    tdEos

    Expression

    Mammalian

    Mutation

    aa 1-82 of NM_001497.3

    Promoter

    CMV

    Available since

    Feb. 5, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

31. pcDNA3.1 myc-mβTrCP WD1-7 (241-569)

    Plasmid

    #62977

    Purpose

    Expresses myc-tagged mouse βTrCP residues 241-569

    Depositor

    Michael Ruppert

    Article

    Deng et al J Cell Sci. 2015 Jan 27. pii: jcs.162164

    Insert

    BTRC (Btrc Mouse)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    codes for residues 241-569

    Promoter

    Available since

    May 28, 2015

    Availability

    Academic Institutions and Nonprofits only

32. pMT-ubb-Avi-Cerulean-RanGAP

    Plasmid

    #79881

    Purpose

    Ubiquitin promoter driving Avi-tagged protein containing Cerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1); flanked by Tol2 sequences

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    Cerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1) (RANGAP1 Chicken)
    

    Use

    Tags

    Avi

    Expression

    Bacterial

    Mutation

    Promoter

    Ubiquitin promoter

    Available since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pLV V5-TbID-LBR

    Plasmid

    #175100

    Purpose

    Lentiviral expression of V5-TbID-tagged mouse Lbr

    Depositor

    Larry Gerace

    Article

    Cheng et al J Proteome Res. 2022 Sep 2;21(9):2197-22

    Insert

    Lbr (Lbr Mouse)
    

    Use

    Lentiviral

    Tags

    V5-TbID

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pcDNA3.1 myc-mβTrCP F-box (1-247)

    Plasmid

    #62976

    Purpose

    Expresses myc-tagged mouse βTrCP residues 1-247

    Depositor

    Michael Ruppert

    Article

    Deng et al J Cell Sci. 2015 Jan 27. pii: jcs.162164

    Insert

    BTRC (Btrc Mouse)
    

    Use

    Tags

    3x myc

    Expression

    Mammalian

    Mutation

    codes for residues 1-247

    Promoter

    Available since

    May 28, 2015

    Availability

    Academic Institutions and Nonprofits only

35. pET-pro-siRNA-V2-LMNA

    Plasmid

    #111088

    Purpose

    pET-pro-siRNA-V2 based plasmid for production of recombinant siRNAs (pro-siRNAs) against human Lamin A/C gene.

    Depositor

    Linfeng HUANG

    Article

    Kaur et al Biotechnol Bioeng. 2018 Jun 5. doi: 10.1

    Insert

    Lamin A/C (LMNA Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pMT-zic2a-NLS-BirA-2A-mCherry_Ras

    Plasmid

    #80065

    Purpose

    Zic2a promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
    

    Use

    Zebrafish biotagging

    Tags

    3x HA

    Expression

    Bacterial

    Mutation

    Promoter

    Zic2a enhancer driving c-fos promoter

    Available since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. tdEos-Actin-C-18

    Plasmid

    #57571

    Purpose

    Localization: Actin, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Michael Davidson

    Article

    Shtengel et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

    Insert

    Actin (ACTB Human)
    

    Use

    Tags

    tdEos

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 16, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

38. pMTB2-Avi-Cerulean-Rpl10a

    Plasmid

    #79880

    Purpose

    Beta-actin2 promoter driving Avi-tagged protein containing Cerulean protein fused to zebrafish Rpl10a ribosomal unit (Tryon et al. 2012); flanked by Tol2 sequences

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    Cerulean protein fused to zebrafish Rpl10a ribosomal unit (rpl10a Zebrafish)
    

    Use

    Tags

    Avi

    Expression

    Bacterial

    Mutation

    Promoter

    Beta-actin2

    Available since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. p3xFLAG-CMV-Drosha (NLS-P426)

    Plasmid

    #171538

    Purpose

    Encodes mutant human Drosha protein

    Depositor

    Yan Zeng

    Article

    Zhang et al Biochim Biophys Acta Gene Regul Mech. 20

    Insert

    DROSHA (DROSHA Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    an SV40 NLS sequence (PKKKRKVGI) insertion into t…

    Promoter

    Available since

    Oct. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pGEM-NLS-BirA-2A-mCherry-SV40pA-FKF

    Plasmid

    #79889

    Purpose

    Donor cassette containing HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), mCherry protein and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    HA-tagged NLS-BirA, 2A, mCherry protein and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pMT-myl7-NLS-BirA-2A-mCherry_Ras

    Plasmid

    #80061

    Purpose

    Myl7 promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
    

    Use

    Zebrafish biotagging

    Tags

    3x HA

    Expression

    Bacterial

    Mutation

    Promoter

    Myl7 promoter

    Available since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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42. pMTB2-RanGAP-Cerulean-Avi

    Plasmid

    #80068

    Purpose

    Beta-actin2 promoter driving C-term Avi-tagged protein containing Cerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1); flanked by Tol2 sequences

    Depositor

    Tatjana Sauka-Spengler

    Article

    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

    Insert

    Cerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1) (RANGAP1 Chicken)
    

    Use

    Zebrafish biotagging

    Tags

    Avi

    Expression

    Bacterial

    Mutation

    Promoter

    Beta-actin2

    Available since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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43. pGEM BirA-2A-Citrine-SV40pA-FRT-Kan-FRT

    Plasmid

    #89890

    Purpose

    BAC donor construct containing 3XHA-tagged BirA, a ribosome skipping motif - 2A, Citrine reporter, polyadenyation signal, followed by FRT recombination sites flanking kanamycin selection cassette.

    Depositor

    Tatjana Sauka-Spengler

    Article

    Kenyon et al Dis Model Mech. 2018 Apr 6;11(4). pii: d

    Insert

    HA-BirA-2A-citrine
    

    Use

    Unspecified

    Tags

    Citrine

    Expression

    Mutation

    Promoter

    Available since

    Aug. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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44. GFP(N-glyc)-Tac-TC

    Plasmid

    #162496

    Purpose

    Expresses partial length Tac (TMD and cytosolic tail) with GFP tag in the N-terminus in mammalian cells, and there is one N-glycosylation site in GFP tag.

    Depositor

    Lei Lu

    Article

    Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014

    Insert

    partial length Tac (TMD and cytosolic tail) (IL2RA Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Tac is in partial length with its TMD and cysotol…

    Promoter

    Available since

    Jan. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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45. pAWp28-v1 scaffold

    Plasmid

    #157976

    Purpose

    pBT264-U6p-{2xBbsI}-v1 sgRNA scaffold-{MfeI} Note: This plasmid is unstable. Screen multiple colonies to identify full length clones.

    Depositor

    Alan Siu Lun Wong

    Article

    Zhou et al Cell Rep. 2020 Aug 11;32(6):108020. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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46. pcDNA3.1 myc-mβTrCP WD2-7 (292-569)

    Plasmid

    #62978

    Purpose

    Expresses myc-tagged mouse βTrCP residues 292-569

    Depositor

    Michael Ruppert

    Article

    Deng et al J Cell Sci. 2015 Jan 27. pii: jcs.162164

    Insert

    BTRC (Btrc Mouse)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    codes for residues 292-569

    Promoter

    Available since

    May 28, 2015

    Availability

    Academic Institutions and Nonprofits only

47. human ETV6 gRNA-4

    Plasmid

    #133404

    Purpose

    human ETV6 gRNA-3 and 4 is a pair of gRNAs. Four ETV6 gRNA plasmids used the same backbone(Addgene plasmid#41824). ETV6 gRNA-4 target the second ETV6 exon.

    Depositor

    Linzhao Cheng

    Article

    Wu et al Stem Cell Res. 2018 May;29:6-14. doi: 10

    Insert

    ETV6 (ETV6 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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48. pAAV-TNT4-Zfr-P2A-HA-luciferase

    Plasmid

    #223188

    Purpose

    Plasmid containing the Zfr/Firefly luciferase cassette. Translation of luciferase is controlled by splicing modulation of the Zfr by risdiplam.

    Depositor

    Yuxuan Guo

    Article

    Chen et al Nat Cardiovasc Res. 2025 Jun 13. doi: 10

    Insert

    luciferase (LOC116160065 firefly)
    

    Use

    AAV

    Tags

    HA and engineered P2A

    Expression

    Mutation

    Promoter

    TNNT2

    Available since

    June 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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49. pCDH-CB-Cdkn1a

    Plasmid

    #228914

    Purpose

    Expression CDKN1A in mammalian cells

    Depositor

    Sika Zheng

    Article

    Lin et al Neuron. 2024 Jul 3;112(13):2157-2176.e12

    Insert

    Cdkn1a (Cdkn1a Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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50. pCAGIG-CDKN1A

    Plasmid

    #228912

    Purpose

    Expression CDKN1A in mammalian cells

    Depositor

    Sika Zheng

    Article

    Lin et al Neuron. 2024 Jul 3;112(13):2157-2176.e12

    Insert

    Cdkn1a (Cdkn1a Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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