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pHAGE-BRAF-V600E

Plasmid

#116204

Purpose

Lentiviral expression of BRAF V600E

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

BRAF (BRAF Human)

Use

Lentiviral

Mutation

V600E

Available Since

Jan. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-sACE2v1-Fc(IgG1)

Plasmid

#145164

Purpose

Mammalian expression plasmid for soluble ACE2-Fc fusion (IgG1) high affinity variant 1

Depositor

Erik Procko

Article

Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

Insert

Angiotensin-converting enzyme 2 (ACE2 Human)

Tags

Fc region of human IgG1

Expression

Mammalian

Mutation

Extracellular, soluble protease domain (a.a. M1-D…

Promoter

CMV

Available Since

April 29, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-puro /BRAF-V600E

Plasmid

#131724

Purpose

Mammalian Expression of Flag tagged BRAF

Depositor

Jacques De Grève

Article

Noeparast et al Oncogene. 2019 Aug;38(31):5933-5941. doi

Insert

BRAF (BRAF Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

V600E

Promoter

CMV

Available Since

Feb. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Lyso-INPP5E

Plasmid

#236004

Purpose

Lysosome-targeted inositol polyphosphate 5 phosphatase (INPP5E); hydrolyzes 5-phosphate in PI(3,4,5)P3 and PI(4,5)P2.

Depositor

Jin Zhang

Article

Sahan et al Proc Natl Acad Sci U S A. 2025 Apr;122(1

Insert

Lyso-INPP5E (LAMP1, INPP5E Synthetic, Human)

Tags

Full-length LAMP1 and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pET-His6-FnCas9GFP

Plasmid

#130966

Purpose

expression of FnCas9-GFP in bacterial cells

Depositor

Debojyoti Chakraborty

Article

Acharya et al Proc Natl Acad Sci U S A. 2019 Oct 15;11

Insert

HA-NLS-FnCas9

Use

CRISPR

Tags

6xHis-mEGFP-TEV-HA-NLS

Expression

Bacterial

Promoter

T7

Available Since

Nov. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC25A13

Plasmid

#132038

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC25A13 (SLC25A13 Human)

Expression

Mammalian

Available Since

Nov. 1, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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CL20_mEGFP_KIAA1549_BRAF

Plasmid

#205838

Purpose

Express mEGFP-tagged fusion protein, KIAA1549_BRAF from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

KIAA1549_BRAF (KIAA1549, BRAF Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-FBXL5-SKP1

Plasmid

#226620

Purpose

Co-expression of FBXL5-SKP1 complex in E.coli

Depositor

Ning Zheng

Article

Wang et al Mol Cell. 2020 Apr 2;78(1):31-41.e5. doi

Inserts

FBXL5 (FBXL5 Human)
SKP1 (SKP1 Human)

Tags

GB1

Expression

Bacterial

Mutation

Amino acids 1-198 was deleted, and 420-596 was re…

Promoter

T7

Available Since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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Beta-2-adrenergic receptor-mRFP

Plasmid

#67145

Purpose

Beta-2-adrenergic receptor tagged with mRFP on carboxyl terminus

Depositor

Catherine Berlot

Article

Hynes et al J Biol Chem. 2004 Oct 15;279(42):44101-1

Insert

Beta-2-adrenergic receptor-mRFP (ADRB2 Human, Discosoma sp.)

Tags

mRFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only

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LgBiT-hACE2

Plasmid

#173431

Purpose

protein expression plasmid of LgBiT-hACE2-IgG1 Fc

Depositor

David Nemazee

Article

Huang et al J Immunol. 2021 Jul 1;207(1):344-351. do

Insert

LgBiT-hACE2-IgG1 Fc (ACE2 Human)

Use

Luciferase

Tags

IgG1

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-BRAF

Plasmid

#116719

Purpose

Lentiviral expression of BRAF

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

BRAF (BRAF Human)

Use

Lentiviral

Mutation

WT

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pGMC00030 (aka pRC0707)

Plasmid

#209673

Purpose

Lentiviral vector expressing PP7-P65-HSF1

Depositor

Raj Chari

Article

Yang et al Sci Adv. 2024 Feb 16;10(7):eadj2445. doi

Insert

PP7-P65-HSF1 (HSF1, RELA Human)

Use

Lentiviral

Expression

Mammalian

Available Since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-U6-dgRNA-CAG-MPH

Plasmid

#106259

Purpose

Expresses MPH co-activator from the CAG promoter and one U6-driven dgRNA in AAV backbone

Depositor

Juan Belmonte

Article

Liao et al Cell. 2017 Dec 14;171(7):1495-1507.e15.

Insert

MS2-P65-HSF1 (HSF1 Synthetic, Human)

Use

AAV and CRISPR

Expression

Mammalian

Promoter

CAG

Available Since

May 2, 2018

Availability

Academic Institutions and Nonprofits only

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pIG-702pUC19-tNGFR-P2A-LRBA

Plasmid

#186096

Purpose

Knockin of truncated NGFR to target gene

Depositor

Alexander Marson

Article

Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

Insert

LRBA (LRBA Human)

Use

CRISPR

Mutation

WT with tNGFR sequence

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR223_BRAF_p.V600E

Plasmid

#81700

Purpose

Gateway Donor vector containing BRAF, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

BRAF (BRAF Human)

Use

Gateway Cloning

Mutation

V600E

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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mRFP-FKBP12-5ptpase domain

Plasmid

#67516

Purpose

Recruitable human type IV 5-phosphatase domain (214-644)

Depositor

Tamas Balla

Article

Varnai et al J Cell Biol. 2006 Nov 6;175(3):377-82.

Insert

FKBP12-5ptpase enzyme (INPP5E, FKBP1A Human)

Tags

mRFP

Mutation

INPP5E has C641A to destroy the C-terminal CAAX d…

Available Since

Sept. 9, 2015

Availability

Academic Institutions and Nonprofits only
pHAGE-BRAF-V600K

Plasmid

#116206

Purpose

Lentiviral expression of BRAF V600K

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

BRAF (BRAF Human)

Use

Lentiviral

Mutation

V600K

Available Since

Jan. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pscALPSpuro-RsACE2

Plasmid

#159665

Purpose

Expresses Rhinolophus sinicus ACE2 in mammalian cells

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

Insert

rhinolophus sinicus ACE2 (ACE2 rhinolophus sinicus)

Use

Lentiviral

Tags

Myc tag

Expression

Mammalian

Promoter

SFFV

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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Beta-2-adrenergic receptor-YFP

Plasmid

#67146

Purpose

Beta-2-adrenergic receptor tagged with YFP on carboxyl terminus

Depositor

Catherine Berlot

Article

Hynes et al J Biol Chem. 2004 Oct 15;279(42):44101-1

Insert

Beta-2-adrenergic receptor-YFP (ADRB2 Aequorea victoria, Human)

Tags

YFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pHAGE-BRAF-K601E

Plasmid

#116168

Purpose

Lentiviral expression of BRAF K601E

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

BRAF (BRAF Human)

Use

Lentiviral

Mutation

K601E

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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