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pDONR223_BRAF_p.V600E

Plasmid

#82186

Purpose

Gateway Donor vector containing BRAF , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

BRAF (BRAF Human)

Use

Gateway entry vector

Mutation

V600E

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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mRFP-FKBP12-5ptpase domain

Plasmid

#67516

Purpose

Recruitable human type IV 5-phosphatase domain (214-644)

Depositor

Tamas Balla

Article

Varnai et al J Cell Biol. 2006 Nov 6;175(3):377-82.

Insert

FKBP12-5ptpase enzyme (INPP5E, FKBP1A Human)

Tags

mRFP

Mutation

INPP5E has C641A to destroy the C-terminal CAAX d…

Available Since

Sept. 9, 2015

Availability

Academic Institutions and Nonprofits only
pHAGE-BRAF-V600K

Plasmid

#116206

Purpose

Lentiviral expression of BRAF V600K

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

BRAF (BRAF Human)

Use

Lentiviral

Mutation

V600K

Available Since

Jan. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-sACE2v2.4(732)-8h

Plasmid

#154103

Purpose

Mammalian expression plasmid for dimeric soluble ACE2 high affinity variant 2.4 (8his tag)

Depositor

Erik Procko

Article

Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

Insert

Angiotensin-converting enzyme 2 (ACE2 Human)

Tags

Short GSG linker and 8his tag

Expression

Mammalian

Mutation

Extracellular, soluble protease domain (a.a. M1-G…

Promoter

CMV

Available Since

June 24, 2020

Availability

Academic Institutions and Nonprofits only

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EGFP-INPP5E-CAAX

Plasmid

#202746

Purpose

express plasma membrane targeted fluorescent protein-tagged enzyme

Depositor

Gerry Hammond

Article

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

EGFP:INPP5E(214-644):HRAS(172-189) (HRAS, INPP5E Human)

Expression

Mammalian

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pHAGE-BRAF-S467L

Plasmid

#116194

Purpose

Lentiviral expression of BRAF S467L

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

BRAF (BRAF Human)

Use

Lentiviral

Mutation

S467L

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223_BRAF_p.N581S

Plasmid

#81687

Purpose

Gateway Donor vector containing BRAF, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

BRAF (BRAF Human)

Use

Gateway entry vector

Mutation

N581S

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR221-SLC25A22_STOP

Plasmid

#161217

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC25A22 (SLC25A22 Human)

Expression

Mammalian

Available Since

June 21, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR223_BRAF_p.G466V

Plasmid

#81710

Purpose

Gateway Donor vector containing BRAF, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

BRAF (BRAF Human)

Use

Gateway entry vector

Mutation

G466V

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223_BRAF_p.L597Q

Plasmid

#81684

Purpose

Gateway Donor vector containing BRAF, part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

BRAF (BRAF Human)

Use

Gateway entry vector

Mutation

L597Q

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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CMV-NanoLuc-BRAF-CAT

Plasmid

#194772

Purpose

Mammalian expression construct encoding the BRAF catalytic (CAT) domain (AA 435-766) with an N-terminal NanoLuc tag.

Depositor

Deborah Morrison

Article

Spencer-Smith et al Mol Cell. 2022 Nov 17;82(22):4262-4276.e

Insert

BRAF catalytic (CAT) domain (AA 435-766) (BRAF Human)

Tags

NanoLuc

Expression

Mammalian

Promoter

CMV51

Available Since

June 20, 2023

Availability

Academic Institutions and Nonprofits only

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CMV-BRAF-REG-Halo

Plasmid

#194771

Purpose

Mammalian expression construct encoding the BRAF regulatory (REG) domain (AA 1-435) with a C-terminal HaloTag.

Depositor

Deborah Morrison

Article

Spencer-Smith et al Mol Cell. 2022 Nov 17;82(22):4262-4276.e

Insert

BRAF regulatory (REG) domain (AA 1-435) (BRAF Human)

Tags

HaloTag

Expression

Mammalian

Promoter

CMV51

Available Since

Feb. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pHAGE-BRAF-G596S

Plasmid

#116159

Purpose

Lentiviral expression of BRAF G596S

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

BRAF (BRAF Human)

Use

Lentiviral

Mutation

G596S

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-FBXL5-SKP1

Plasmid

#226620

Purpose

Co-expression of FBXL5-SKP1 complex in E.coli

Depositor

Ning Zheng

Article

Wang et al Mol Cell. 2020 Apr 2;78(1):31-41.e5. doi

Inserts

FBXL5 (FBXL5 Human)
SKP1 (SKP1 Human)

Tags

GB1

Expression

Bacterial

Mutation

Amino acids 1-198 was deleted, and 420-596 was re…

Promoter

T7

Available Since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE-BRAF-G469A

Plasmid

#116153

Purpose

Lentiviral expression of BRAF G469A

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

BRAF (BRAF Human)

Use

Lentiviral

Mutation

G469A

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-BRAF-K601E

Plasmid

#116168

Purpose

Lentiviral expression of BRAF K601E

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

BRAF (BRAF Human)

Use

Lentiviral

Mutation

K601E

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223_EIF1AX_p.G9R

Plasmid

#81282

Purpose

Gateway Donor vector containing EIF1AX , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

EIF1AX (EIF1AX Human)

Use

Gateway entry vector

Mutation

N4D, G9R

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pHAGE-BRAF-W531C

Plasmid

#116210

Purpose

Lentiviral expression of BRAF W531C

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

BRAF (BRAF Human)

Use

Lentiviral

Mutation

W531C

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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Beta-2-adrenergic receptor-mCerulean

Plasmid

#66957

Purpose

Beta-2-adrenergic receptor tagged with mCerulean on carboxyl terminus

Depositor

Catherine Berlot

Article

Hynes et al J Biol Chem. 2004 Oct 15;279(42):44101-1

Insert

Beta-2-adrenergic receptor-mCerulean (ADRB2 Aequorea victoria, Human)

Tags

mCerulean

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_MKRN1_BRAF

Plasmid

#205857

Purpose

Express mEGFP-tagged fusion protein, MKRN1_BRAF from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

MKRN1_BRAF (MKRN1, BRAF Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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