We narrowed to 7,125 results for: trac

1. pTBL322 GC

   Plasmid

   #126950

   Purpose

   To target a protospacer with GC at the cut site.

   Depositor

   Chang Liu

   Article

   Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

   Insert

   spacer against human genome with GC at cut site
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human U6

   Available since

   May 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pTBL581 GA

   Plasmid

   #126951

   Purpose

   To target a protospacer with GA at the cut site.

   Depositor

   Chang Liu

   Article

   Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

   Insert

   spacer against human genome with GA at cut site
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human U6

   Available since

   May 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pTBL601 AG

   Plasmid

   #126952

   Purpose

   To target a protospacer with AG at the cut site.

   Depositor

   Chang Liu

   Article

   Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

   Insert

   spacer against human genome with AG at cut site
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human U6

   Available since

   May 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pTBL602 CG

   Plasmid

   #126953

   Purpose

   To target a protospacer with CG at the cut site.

   Depositor

   Chang Liu

   Article

   Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

   Insert

   spacer against human genome with CG at cut site
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human U6

   Available since

   May 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGEX-4T-3-cytXlATL2-R232Q

   Plasmid

   #124064

   Purpose

   Control protein. The R232Q mutation abolishes dimerization activity, so this construct is inactive compared to wild type cytATL

   Depositor

   Tom Rapoport

   Article

   Wang et al J Cell Biol. 2013 Dec 9;203(5):801-14. d

   Insert

   cytXlATL2-R232Q
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   May 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pFA6a-kanMX6-P3nmt1-mECitrine

   Plasmid

   #105151

   Purpose

   Yeast gene targeting

   Depositor

   Jian-Qiu Wu

   Article

   Ye et al Mol Biol Cell. 2012 Apr;23(7):1181-95. d

   Insert

   kanMX6-P3nmt1-mECitrine
   

   Use

   Tags

   3nmt1-mECitrine

   Expression

   Bacterial and Yeast

   Mutation

   F64L, Q69M, A206K

   Promoter

   3nmt1

   Available since

   March 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pVH009

   Plasmid

   #80399

   Purpose

   Control plasmid with no anti-scaffold feedback. Contains pBAD-CusR (response regulator) and pCusR-YFP-AAV.

   Depositor

   Richard Murray

   Article

   Hsiao et al ACS Synth Biol. 2015 Feb 20;4(2):150-61.

   Inserts

   3xFLAG-Venus YFP-AAV
   CusR (cusR )
   AraC (araC )
   

   Use

   Synthetic Biology

   Tags

   C-terminal LZx domain and N-terminal 3xFLAG tag, …

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pVH015

   Plasmid

   #80398

   Purpose

   Contains pBAD-CusR (response regulator) and pCusR-antiscaffold-GFP(3xFLAG). Used for Western blots

   Depositor

   Richard Murray

   Article

   Hsiao et al ACS Synth Biol. 2015 Feb 20;4(2):150-61.

   Inserts

   Anti-scaffold-ffGFP-3xFLAG
   CusR (cusR )
   AraC (araC )
   

   Use

   Synthetic Biology

   Tags

   C-terminal 3xFLAG tag and C-terminal LZx domain

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET30-SBL1(160S,HisTagDel)

   Plasmid

   #66880

   Purpose

   Expresses a version of pET30Xa-SBL1(160S) with 41 bp Histag removed

   Depositor

   Betty Gaffney

   Article

   Coffa et al J Biol Chem. 2005 Nov 18;280(46):38756-6

   Insert

   soybean lipoxygenase-1 (S160S)
   

   Use

   Tags

   none

   Expression

   Bacterial

   Mutation

   none

   Promoter

   T7lac

   Available since

   Aug. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET30-SBL1(160E,HisTagDel)

    Plasmid

    #66879

    Purpose

    Expresses a version of pET30Xa-SBL1(160E) with 41 bp Histag removed

    Depositor

    Betty Gaffney

    Article

    Coffa et al J Biol Chem. 2005 Nov 18;280(46):38756-6

    Insert

    soybean lipoxygenase-1 (S160E)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    S160E

    Promoter

    T7lac

    Available since

    Aug. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAV10

    Plasmid

    #63213

    Purpose

    pUC19 backbone (Amp), contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. NotI or FseI sites for TU digest.

    Depositor

    Jef Boeke

    Article

    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET-28a_6H-TAQ_E602D

    Plasmid

    #166944

    Purpose

    Bacterial expression of Taq DNA polymerase

    Depositor

    Robert Tjian

    Article

    Graham et al PLoS One. 2021 Feb 3;16(2):e0246647. doi

    Insert

    Thermus aquaticus DNA polymerase
    

    Use

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    E602D

    Promoter

    T7

    Available since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. AAV-hSYN-eGFP

    Plasmid

    #162512

    Purpose

    AAV vector plasmid expressing enhanced green fluorescent protein (eGFP) under the human synapsin (hSYN) promoter

    Depositor

    Bart Nieuwenhuis

    Article

    Nieuwenhuis et al Gene Ther. 2020 Jun 23. pii: 10.1038/s41

    Insert

    eGFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSYN

    Available since

    April 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCFJ2437 - [1-2] ENTR - Fluor - ce-tagRFP-T(PATCs(900bp), NLS, no_atg, no stop)

    Plasmid

    #159857

    Purpose

    Three-fragment Gateway compatible flourophore for expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [1-2] ENTR - Fluor - ce-tagRFP-T(PATCs(900bp), NLS, no_atg, no stop)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCFJ2451 - [1-2] ENTR - Fluor - ce-tagBFP2(PATCs(900bp), NLS, no_atg, no stop)

    Plasmid

    #159845

    Purpose

    Three-fragment Gateway compatible flourophore for expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [1-2] ENTR - Fluor - ce-tagBFP2(PATCs(900bp), NLS, no_atg, no stop)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEGFP.90 beta

    Plasmid

    #108221

    Purpose

    Mammalian expression vector for EGFP fusion to human Hsp90 beta

    Depositor

    Didier Picard

    Article

    Picard et al Exp Cell Res. 2006 Nov 15. 312(19):3949-

    Insert

    EGFP-Hsp90b (HSP90AB1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDestTol2pA2-9xGRE

    Plasmid

    #74736

    Purpose

    GRE-dependent EGFP reporter construct flanked by the minimal Tol2 transposon elements

    Depositor

    Luisa Dalla Valle

    Article

    Benato et al Mol Cell Endocrinol. 2014 Jul 5;392(1-2)

    Insert

    EGFP
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    nine GC-responsive elements (9xGRE) from the rat …

    Available since

    April 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRK5 alpha6

    Plasmid

    #16036

    Depositor

    Filippo Giancotti

    Article

    Dans et al J Biol Chem. 2001 Jan 12. 276(2):1494-50

    Insert

    alpha6 (ITGA6 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. PGK-H2BmCherry

    Plasmid

    #21217

    Depositor

    Mark Mercola

    Article

    Kita-Matsuo et al PLoS ONE. 2009 . 4(4):e5046.

    Insert

    Phosphoglycerate kinase promoter (PGK1 Human)
    

    Use

    Lentiviral

    Tags

    H2B-mCherry

    Expression

    Mutation

    Promoter

    Available since

    July 1, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3-N-mSOG-FRB-IRES-mCherry

    Plasmid

    #136602

    Purpose

    Encodes FRB tagged with the N-terminal fragment of split-miniSOG (mSOG1-94).

    Depositor

    John Ngo

    Article

    Boassa et al Cell Chem Biol. 2019 Oct 17;26(10):1407-

    Insert

    N-mSOG-FRB
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge