We narrowed to 4,347 results for: ARA-2

1. pJL1-AcrA

   Plasmid

   #198214

   Purpose

   pJL1 plasmid encoding wildtype AcrA from C. jejuni containing 2 native internal glycosylation sites recognized by PglB and a C-terminal 6x-His tag

   Depositor

   Michael Jewett

   Article

   Hershewe et al Nat Commun. 2021 Apr 22;12(1):2363. doi:

   Insert

   AcrA
   

   Use

   Tags

   6x His tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. SP_H6_Halo_L172Q_KDEL_pBABEpu

   Plasmid

   #183690

   Purpose

   Retroviral expression of ER-targeted metastable Halotag/folding sensor (ER HaloL172Q)

   Depositor

   Edward Avezov

   Article

   Melo et al Nat Commun. 2022 May 6;13(1):2501. doi:

   Insert

   ER Halo(L172Q)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   L72Q in Halotag

   Promoter

   Available since

   April 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. SP_H6_Halo_M21K_F86L_KDEL_pBABEpu

   Plasmid

   #183691

   Purpose

   Retroviral expression of ER-targeted metastable Halotag/folding sensor (ER HaloM21K_F86L)

   Depositor

   Edward Avezov

   Article

   Melo et al Nat Commun. 2022 May 6;13(1):2501. doi:

   Insert

   ER Halo(M21K_F86L)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   M21K_F86L in Halotag

   Promoter

   Available since

   April 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET30_Halotag_K73T_Jdomain

   Plasmid

   #183693

   Purpose

   Bacterial expression of ER-targeted metastable Halotag/folding sensor (HaloK73T) fused with J domain

   Depositor

   Edward Avezov

   Article

   Melo et al Nat Commun. 2022 May 6;13(1):2501. doi:

   Insert

   ER Halo(K73T)-J domain
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   K73T in Halotag

   Promoter

   Available since

   April 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLz77_CB_operon_CsoS2_N3

   Plasmid

   #140859

   Purpose

   Same as pLz37 but with CsoS2 discontinuously truncated such that repeats N1, N2, and N4 are removed

   Depositor

   David Savage

   Article

   Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

   Insert

   CbbL, CbbS, CsoS2 (delN1-N2, N4), CsoSCA, CsoS4A, CsoS4B, CsoS1C, CsoS1A, CsoS1B, CsoS1D
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-108 and 220-235 of CsoS2

   Promoter

   Available since

   June 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLz78_CB_operon_CsoS2_delNTD

   Plasmid

   #140860

   Purpose

   Same as pLz37 but with CsoS2 truncated to remove the entire NTD (i.e. N1, N2, N3, and N4 are removed)

   Depositor

   David Savage

   Article

   Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

   Insert

   CbbL, CbbS, CsoS2 (delNTD), CsoSCA, CsoS4A, CsoS4B, CsoS1C, CsoS1A, CsoS1B, CsoS1D
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-235 of CsoS2

   Promoter

   Available since

   June 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDONR-IAA19_no stop

   Plasmid

   #83857

   Purpose

   Gateway donor vector for IAA19 with no stop codon

   Depositor

   Jennifer Nemhauser

   Article

   Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

   Insert

   IAA19 (IAA19 Mustard Weed)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   no stop codon

   Promoter

   Available since

   Oct. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDONR-IAA5

   Plasmid

   #83867

   Purpose

   Gateway donor vector for IAA5

   Depositor

   Jennifer Nemhauser

   Article

   Havens et al Plant Physiol. 2012 Sep;160(1):135-42. d

   Insert

   NP_173011.1 (IAA5 Mustard Weed)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. psiCHECK RHEBL1 3'UTR

   Plasmid

   #19858

   Depositor

   Thomas Tuschl

   Article

   Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

   Insert

   RHEBL1 3'UTR (RHEBL1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUDR295

    Plasmid

    #113874

    Purpose

    expression of Cas9 programming sgRNA2 and sgRNA1 targetting GAL2 and HXT4-1-5/ HXT3-6-7 respectively

    Depositor

    Jean-Marc Daran, Jack Pronk

    Article

    Wijsman et al FEMS Yeast Res. 2019 Jan 1;19(1). pii: 5

    Insert

    sgRNA2 GAL2 sgRNA1-HXT4-1-5;HXT3-6-7
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pICH47742::p35S-PE2max-NC::tEURb7

    Plasmid

    #227693

    Purpose

    Golden Gate level 1, position 2, PE2max-NC expression cassette.

    Depositor

    Jae-Yean Kim

    Article

    Vu et al Nat Plants. 2024 Sep 6. doi: 10.1038/s41

    Insert

    PE2max-NC being driven by long CaMV 35S (p35S) and terminated by a dual terminator (EURb7).
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV 35S

    Available since

    Nov. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. HDAC3 Flag

    Plasmid

    #13819

    Purpose

    Mammalian expression of human histone deacetylase 3 with flag tag

    Depositor

    Eric Verdin

    Article

    Emiliani et al Proc Natl Acad Sci U S A. 1998 Mar 17. 9

    Insert

    HDAC3 (HDAC3 Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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13. -2.5Kb MMP10

    Plasmid

    #53973

    Purpose

    Luciferase reporter for mouse MMP10 2.5kb promoter

    Depositor

    Michael Chin

    Article

    Wu et al Biochem Biophys Res Commun. 2011 Dec 2;4

    Insert

    mouse MMP10 2.5Kb promoter (Mmp10 Mouse)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PCR from C57BL6/J

    Available since

    June 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. piRFP-N3-Tyrosinase

    Plasmid

    #80152

    Purpose

    Melanosome marker

    Depositor

    Santiago Di Pietro

    Article

    Ambrosio et al Proc Natl Acad Sci U S A. 2016 May 17;11

    Insert

    Tyrosinase (TYR Human)
    

    Use

    Tags

    iRFP682

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 29, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pFCIV-CEACAM6

    Plasmid

    #149303

    Purpose

    lentiviral packaging vector for CEACAM6 expression.

    Depositor

    James Fleckenstein

    Article

    Sheikh et al Proc Natl Acad Sci U S A. 2020 Nov 17;11

    Insert

    CEACAM6 (CEACAM6 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    ubiquitin C

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. linker-mCitrine-PIF6-NAT

    Plasmid

    #51576

    Purpose

    Tag protein of interest with PIF6 in Saccharomyces cerevisiae

    Depositor

    Chao Tang

    Article

    Yang et al Mol Biol Cell. 2013 Aug;24(15):2419-30.

    Insert

    PIF6 (PIL2 Mustard Weed)
    

    Use

    Tags

    linker and mCitrine

    Expression

    Yeast

    Mutation

    1-100aa of PIF6

    Promoter

    none

    Available since

    Oct. 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. MELOPS

    Plasmid

    #80151

    Purpose

    Genetically encoded melanosome-localized pH sensor.

    Depositor

    Santiago Di Pietro

    Article

    Ambrosio et al Proc Natl Acad Sci U S A. 2016 May 17;11

    Insert

    OCA2 (OCA2 Human)
    

    Use

    Tags

    mNectarine in first luminal loop

    Expression

    Mammalian

    Mutation

    nonpathologic polymorphism D257A, pore mutation V…

    Promoter

    CMV

    Available since

    Sept. 20, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

18. DR5rev::erRFP

    Plasmid

    #61011

    Purpose

    Auxin signalling reporter (DR5rev) driving RFP (ER-targeted)

    Depositor

    Alexis Maizel

    Article

    Marin et al Plant Cell. 2010 Apr;22(4):1104-17. doi:

    Insert

    DR5rev::erRFP:35S_term
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    DR5rev::erRFP:35Sterm

    Available since

    Feb. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCIFR-3

    Plasmid

    #233295

    Purpose

    Integration of msfGFP (or your Module Of Interest) in a random fashion via Tn5 insertion. The antibiotic cassette used for selecting for the integration can be removed in a later stage with pFNC.

    Depositor

    Pablo Ivan Nikel

    Article

    Federici et al Metab Eng. 2025 Jan 6;88:180-195. doi: 1

    Insert

    msfGFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    p14G

    Available since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSLQ7334: pHR-PGK-SV40_NLS-dCas12f-VPR-c- Myc_NLS-mCherry-WPRE

    Plasmid

    #176268

    Purpose

    Expression of dCas12f-VPR-mCherry in mammalian cells

    Depositor

    Stanley Qi

    Article

    Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

    Insert

    dCas12f
    

    Use

    Lentiviral

    Tags

    HA-VPR-mCherry

    Expression

    Mammalian

    Mutation

    D326A, D510A

    Promoter

    PGK

    Available since

    Oct. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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21. pKK233-hCaM

    Plasmid

    #47598

    Depositor

    Emanuel Strehler

    Article

    Rhyner et al Biochemistry. 1992 Dec 29;31(51):12826-3

    Insert

    CaM (CALM3 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    contains 469 bp fragment (nt 92-559)

    Promoter

    trc

    Available since

    Aug. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pTpal-fdc

    Plasmid

    #78288

    Purpose

    Expresses A. thaliana PAL2 and S. cerevisiae FDC1 in E. coli

    Depositor

    David Nielsen

    Article

    McKenna et al Biotechnol J. 2013 Dec;8(12):1465-75. do

    Inserts

    PAL2 (PAL2 Mustard Weed)
    FDC1 (FDC1 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    *see below

    Promoter

    trc

    Available since

    June 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET28-At_PRORP2-His

    Plasmid

    #67870

    Purpose

    bacterial expression of PRORP2 (At2g16650), full coding sequence + C-term. His tag

    Depositor

    Walter Rossmanith

    Article

    Pavlova et al Chembiochem. 2012 Oct 15;13(15):2270-6.

    Insert

    PRORP2 (PRORP2 Mustard Weed)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA3-HA-ERK2 L198A L232A

    Plasmid

    #8978

    Depositor

    John Blenis

    Article

    Dimitri et al Curr Biol. 2005 Jul 26. 15(14):1319-24.

    Insert

    ERK2 (Mapk1 Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    L198A, L232A

    Promoter

    Available since

    Nov. 2, 2005

    Availability

    Academic Institutions and Nonprofits only

25. pAFMW-AthHEN1

    Plasmid

    #104980

    Purpose

    Expression of FLAG Myc tagged A.thaliana-Hen1 (codon optimised for Drosophila) in Drosophila S2 cells

    Depositor

    Stefan Ameres

    Article

    Alberti et al Nat Methods. 2018 Feb 26. pii: nmeth.461

    Insert

    Hen1 (HEN1 Mustard Weed)
    

    Use

    Tags

    FLAG Myc

    Expression

    Insect

    Mutation

    Promoter

    Actin5C

    Available since

    Jan. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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26. pLBM0090

    Plasmid

    #172824

    Purpose

    Level 2 module containing mCHERRY-nVENUS HSP21-cVENUS

    Depositor

    Benjamin Field

    Article

    Velay et al Plant Methods. 2022 May 26;18(1):69. doi

    Insert

    [35S-P19][35S-CTPSSU-mHSP21-nVENUS][35S-CTPSSU-mHSP21-X3FLAGcVENUS][35S-SP_SV40-CFP]
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pEPQD1CB0121

    Plasmid

    #177053

    Purpose

    MoClo Level 1, position 2, transcriptional unit for transient expression of iridoid oxidase; CYP76A26 (CrIO) from Catharanthus roseus driven by 35S promoter

    Depositor

    Nicola Patron

    Article

    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

    Insert

    iridoid oxidase; CYP76A26 (CrIO) from Catharanthus roseus
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pPN022

    Plasmid

    #91668

    Purpose

    Express sgRNA targeting human SHANK3

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

    Insert

    SHANK3 (SHANK3 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pPN021

    Plasmid

    #91667

    Purpose

    Express sgRNA targeting human SHANK3

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

    Insert

    SHANK3 (SHANK3 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLz74_Rubisco_CsoS2_Npeptide_fusion

    Plasmid

    #140840

    Purpose

    Same as pBz15 but with N*-peptide fusion on CbbL, construct used for protein crystallization

    Depositor

    David Savage

    Article

    Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

    Insert

    Form IA Rubisco with CsoS2 N-peptide fusion; CbbL-Npeptide, CbbS
    

    Use

    Tags

    CsoS2 N-peptide consensus to CbbL C-terminus and …

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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