We narrowed to 3,796 results for: Mak
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pcDNA3-PKAsub-YPet-NES
Plasmid#138203PurposeEncodes C-terminal (substrate) fragment of FRET-based bimolecular PKA activity reporter (BimAKAR); cytosol targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES.DepositorInsertPKAsub-YPet-NES
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianPromoterCMVAvailable SinceApril 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
HOXA9 (murine) FLAG pET
Plasmid#8494DepositorInsertHOXA9 (Hoxa9 Mouse)
TagsFLAG and HisExpressionBacterialMutationFLAG epitope tag replaces T7 tag in the pET back…Available SinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
pVH028
Plasmid#80393PurposeContains constitutive histidine kinase (pCon-Taz) with 4x SH3 scaffold domains + salicylate inducible phosphatase (Psal-CusSmut)DepositorUseSynthetic BiologyTagsFused by GS linkers to 4 SH3 domainsExpressionBacterialMutationGlycine 448 changed to Alanine, makes it primaril…Available SinceNov. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
HOXB13 (human) HIS-tag pET
Plasmid#8543DepositorInsertHOXB13 (HOXB13 Human)
TagsHis and T7ExpressionBacterialMutationmakes protein by Western and by transcription/tra…Available SinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mCherry-PKAsub-RLuc8N-NES
Plasmid#138211PurposeEncodes substrate fragment of luminescence-based bimolecular PKA activity reporter (LumAKAR); cytosol targeted. Use in conjunction with pcDNA3-RLuc8C-FHA1-NES.DepositorInsertmCherry-PKAsub-RLuc8N-NES
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianPromoterCMVAvailable SinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBEST_OR2-OR1-Pr_UTR1-deGFP-T500 BsaI, BbsI-safe (105p)
Plasmid#70194PurposeDerived from 21 with silent mutations to make BsaI, BbsI compatibleDepositorInsertdeGFP
Available SinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
HOXB13 (human) Myc-tag-GST
Plasmid#8683DepositorInsertHOXB13 (HOXB13 Human)
TagsGST and MycExpressionBacterialMutationmakes protein by westernAvailable SinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
HOXC13(human) GSTfusion-pET
Plasmid#8680DepositorInsertHOXC13 (HOXC13 Human)
TagsGSTExpressionBacterialMutationmakes protein by Coomassie blue stainingAvailable SinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
HOXD13 (chicken) HIS-tag pET
Plasmid#8555DepositorInsertHOXD13 (HOXD13 )
TagsHis and T7ExpressionBacterialMutationcontains 10 nt 5' to ATG start; makes prote…Available SinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
pcDNA 5/FR/TO-Ubl4A K48R
Plasmid#61811Purposeexpress C-terminal Ubl4A K48R, for making the stable cell lineDepositorAvailable SinceFeb. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
HOXB4-NA-MSCV
Plasmid#20987DepositorInsertHOXB4 (HOXB4 Human)
UseRetroviralExpressionMammalianMutationN51A (makes DNA binding mutant protein)Available SinceAug. 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
pLV-Puro-CMV-EPB41L4A-AS1-unspliced-MUT- deltaACAbox
Plasmid#242753PurposeExpresses unspliced EPB41L4A-AS1 in mammalian cellsDepositorInsertEPB41L4A-AS1 (EPB41L4A-AS1 Human)
UseLentiviralExpressionMammalianMutationAACTTAAAAGCAGCGT → ACCTTAGAAGTAGAGT making it res…PromoterCMVAvailable SinceSept. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV-Puro-CMV-EPB41L4A-AS1-unspliced-MUT- deltaHbox
Plasmid#242752PurposeExpresses unspliced EPB41L4A-AS1 in mammalian cellsDepositorInsertEPB41L4A-AS1 (EPB41L4A-AS1 Human)
UseLentiviralExpressionMammalianMutationAACTTAAAAGCAGCGT → ACCTTAGAAGTAGAGT making it res…PromoterCMVAvailable SinceSept. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLV-Puro-CMV-EPB41L4A-AS1 unspliced-MUT- deltaSNORA13
Plasmid#242751PurposeExpresses unspliced EPB41L4A-AS1 in mammalian cellsDepositorInsertEPB41L4A-AS1 (EPB41L4A-AS1 Human)
UseLentiviralExpressionMammalianMutationAACTTAAAAGCAGCGT → ACCTTAGAAGTAGAGT making it res…PromoterCMVAvailable SinceSept. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pIVT-Bxb1-I87L, A369P, E434G
Plasmid#232750PurposeIVT template for making modRNA encoding mutant Bxb1modRNA (I87L, A369P, E434G)DepositorInsertBxb1
UseIn vitro transcriptionTagsHA and SV40 NLSMutationI87L, A369P, E434GAvailable SinceMarch 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
SiRES COR1C DCC-GFP
Plasmid#195149Purposeimage COR1CDepositorInsertCORO1C (CORO1C Human)
ExpressionMammalianMutationmutations to make si RNA resistant and remove coi…Available SinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
SiRES COR1C DCC -Halo
Plasmid#195150Purposeimage COR1CDepositorInsertCORO1C (CORO1C Human)
ExpressionMammalianMutationmutations to make si RNA resistant and remove coi…Available SinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
SiRES COR1C ACT--GFP
Plasmid#195151Purposeimage COR1CDepositorInsertCORO1C (CORO1C Human)
ExpressionMammalianMutationmutations to make si RNA resistant and remove act…Available SinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
SiRES COR1C ACT--Halo
Plasmid#195152Purposeimage COR1CDepositorInsertCORO1C (CORO1C Human)
ExpressionMammalianMutationmutations to make si RNA resistant and remove act…Available SinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-ATG101
Plasmid#189590PurposeTo make ATG101/ATG13 HORMA complexDepositorAvailable SinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
NeuA.pJL1
Plasmid#199115PurposeIn vitro expression of N-acylneuraminate cytodiylyltransfease (NeuA), which conjugates sialic acid and CTP to make activated sugar donor, CMP-sialic acid (see CSTI.PJL1 and PdST6.PJL1)DepositorInsertN-acylneuraminate cytodiylyltransfease (NeuA)
TagsStrep-tagExpressionBacterialPromoterT7Available SinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYL237
Plasmid#173194PurposeCRISPR donor plasmid for making the RNA-binding mutant EZH2 control cell line. It contains a puromycin resistance gene and an mCherry geneDepositorInsertEZH2 RNA-binding mutant
UseCRISPRExpressionMammalianAvailable SinceApril 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDSARN-vasa-eSpCas9sv40
Plasmid#173670PurposeeSpCas9 expression vector for AnophelesDepositorInserteSpCas9 with Anopheles vasa promoter and SV40 terminator
UseCRISPRExpressionInsectMutationmutations in Cas9 reducing off-target activity (S…PromotervasaAvailable SinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-myc-Sema4A RNAiR
Plasmid#115172Purposeexpresses RNAi-resistant Sema4A with N-terminal myc tagDepositorInsertSema4A (Sema4a Mouse)
TagsmycExpressionMammalianMutationSilent mutation between amino acids in ORF (401-4…PromoterCMVAvailable SinceDec. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
MSCV-6NBC-GFP-PGK-Puro-Barcode81
Plasmid#83758Purposeindividual 6N barcode to make barcoded cell line variantsDepositorInsertBarcode81
UseRetroviralMutationdeletion of T in starting ATG of GFP (please see …Available SinceNov. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
MSCV-6NBC-GFP-PGK-Puro-Barcode71
Plasmid#83748Purposeindividual 6N barcode to make barcoded cell line variantsDepositorInsertBarcode71
UseRetroviralMutationdeletion of AA5-233 in GFP (please see depositor …Available SinceNov. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-PKAsub-YPet-Kras
Plasmid#138206PurposeEncodes C-terminal (substrate) fragment of FRET-based bimolecular PKA activity reporter (bimAKAR); plasma membrane targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES.DepositorInsertPKAsub-YPet-Kras
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pLenti-DsRed_IRES_EGFP
Plasmid#92194PurposeLentiviral overexpression vector to make stable bicistronic cell line for control (EGFP) screenDepositorInsertDsRed-IRES-EGFP
UseLentiviralExpressionMammalianAvailable SinceJune 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLC242-GFP-metap2
Plasmid#100518PurposeExpresses GFP tagged metap2 in mammalian cells to make lentivirusDepositorAvailable SinceNov. 10, 2017AvailabilityAcademic Institutions and Nonprofits only
