We narrowed to 44,084 results for: cha

1. pLenti-BE3RA-P2A-Puro

   Plasmid

   #110839

   Purpose

   Lentiviral vector for constitutive expression of BE3RA in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   BE3RA
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   D10A

   Promoter

   EF1s

   Available Since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. LIC-Z

   Plasmid

   #153543

   Purpose

   perform light induced optogenetic clustering of TCR ζ-Chain as described in the associated publication

   Depositor

   Katharina Gaus

   Article

   Ma et al Int J Mol Sci. 2020 May 15;21(10). pii:

   Insert

   human TCR ζ-Chain (CD247 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   NA

   Promoter

   CMV

   Available Since

   July 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3.1-Muc5b D1 (murine) with cleavable His tag

   Plasmid

   #181814

   Purpose

   Expresses murine Muc5b D1 with cleavable His tag, residues 50-426

   Depositor

   Deborah Fass

   Article

   Reznik et al Cell. 2022 Oct 27;185(22):4206-4215.e11.

   Insert

   Muc5b D1 (Muc5b Mouse)
   

   Use

   Tags

   TEV cleavage site followed by 6xHistidine tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pEVA1

   Plasmid

   #58953

   Purpose

   Contains a synthetic DNA sequence, VAR1u, that specifies the Var1 protein. The Cox4 targeting signal is fused to the N-terminus to direct import of Var1. Complements var1 mutations in mtDNA.

   Depositor

   Thomas Mason, Thomas Fox

   Article

   Sanchirico et al EMBO J. 1998 Oct 1;17(19):5796-804.

   Insert

   VAR1u, VAR1 recoded for expression from the nucleus
   

   Use

   Yeast ecoli shuttle vector

   Tags

   preCox4 mitochondrial matrix targeting signal

   Expression

   Yeast

   Mutation

   Synthetic gene in standard genetic code

   Promoter

   ADH1

   Available Since

   Aug. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pET28a-ybbr-HIS-ddFLN4-K10

   Plasmid

   #101718

   Purpose

   K10 peptide with ddFLN4 fingerprint domain and N-terminal ybbr tag for covalent immobilization - resulting in high forces

   Depositor

   Hermann Gaub

   Article

   Milles et al Science. 2018 Mar 30;359(6383):1527-1533

   Insert

   ddFLN4 fingerprint, K10 peptide (KRT10 Dictyostelium discoideum, Human)
   

   Use

   Tags

   HIS and ybbr

   Expression

   Bacterial

   Mutation

   C18S in ddFLN4

   Promoter

   T7

   Available Since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. sgRNA BRCA1 E638K

   Plasmid

   #139327

   Purpose

   Plasmid expressing a sgRNA to introduce BRCA1 E638K using base editing

   Depositor

   Alberto Ciccia

   Article

   Billon et al Cell Rep. 2020 Mar 10;30(10):3280-3295.e

   Insert

   sgRNA to insert BRCA1 E638K using base editing
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   May 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLenti-BE3RA-PGK-Puro

   Plasmid

   #110843

   Purpose

   Lentiviral vector for constitutive expression of BE3RA in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   BE3RA
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   D10A

   Promoter

   EF1s

   Available Since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. sgRNA BRCA2 V572I

   Plasmid

   #139321

   Purpose

   Plasmid expressing a sgRNA to introduce BRCA2 V572I using base editing

   Depositor

   Alberto Ciccia

   Article

   Billon et al Cell Rep. 2020 Mar 10;30(10):3280-3295.e

   Insert

   sgRNA to insert BRCA2 V572I using base editing
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   May 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pRF+275Dux4

   Plasmid

   #21293

   Depositor

   Stephen Tapscott

   Article

   Snider et al Hum Mol Genet. 2009 Jul 1;18(13):2414-30

   Insert

   DUX 4 (DUX4 DUX 4, Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 22, 2009

   Availability

   Academic Institutions and Nonprofits only

10. pcDNA3.1-MUC2 D1D2D'D3 with cleavable His tag

    Plasmid

    #187994

    Purpose

    Expresses MUC2 D1D2D'D3 assembly with cleavable His tag in mammalian cells

    Depositor

    Deborah Fass

    Article

    Reznik et al Cell. 2022 Oct 27;185(22):4206-4215.e11.

    Insert

    mucin2 D1D2D'D3 (MUC2 Human)
    

    Use

    Tags

    6xHistadine followed by TEV cleavage site

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pmCherry-miR-125b-1

    Plasmid

    #58990

    Purpose

    MSCV based expression vector - co-expresses mCherry and human miR-125b-1

    Depositor

    David Baltimore

    Article

    So et al Blood. 2014 Aug 28;124(9):1502-12. doi:

    Inserts

    mCherry fluorescent protein
    miR-125b-1 (MIR125B1 Human)
    

    Use

    Retroviral; Mscv-based retroviral system

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    MSCV

    Available Since

    Oct. 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET28a-ybbr-HIS-ddFLN4-Fgß

    Plasmid

    #101719

    Purpose

    wildtype Fgß peptide with ddFLN4 fingerprint domain with N-terminal ybbr tag for covalent immobilization - resulting in weak forces

    Depositor

    Hermann Gaub

    Article

    Milles et al Science. 2018 Mar 30;359(6383):1527-1533

    Insert

    ddFLN4 fingerprint, Fgß peptide (FGB Dictyostelium discoideum, Human)
    

    Use

    Tags

    HIS and ybbr

    Expression

    Bacterial

    Mutation

    C18S in ddFLN4

    Promoter

    T7

    Available Since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. nuc-yHS1-M7A

    Plasmid

    #159167

    Purpose

    Nuclear labile heme reporter

    Depositor

    Amit Reddi

    Article

    Hanna et al Proc Natl Acad Sci U S A. 2016 Jul 5;113

    Insert

    nuc-yHS1-M7A
    

    Use

    Tags

    SV40 NLS

    Expression

    Yeast

    Mutation

    Mutated Met 7 of the cyt b562 module of nuc-yHS1 …

    Promoter

    GPD

    Available Since

    Sept. 21, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. NET37 pEGFP-N2 (607)

    Plasmid

    #61990

    Purpose

    mammalian expression of nuclear envelope transmembrane protein

    Depositor

    Eric Schirmer

    Article

    Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

    Insert

    NET37 (KIAA1161 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pCS2-mqgDUX4

    Plasmid

    #21176

    Depositor

    Stephen Tapscott

    Article

    Snider et al Hum Mol Genet. 2009 Jul 1;18(13):2414-30

    Insert

    DUX4 (DUX4 Human)
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available Since

    July 22, 2009

    Availability

    Academic Institutions and Nonprofits only

16. pAWp92

    Plasmid

    #157975

    Purpose

    pBT264-mutH1p-{2xBbsI}-sgRNA scaffold-{MfeI}

    Depositor

    Alan Siu Lun Wong

    Article

    Zhou et al Cell Rep. 2020 Aug 11;32(6):108020. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFUW-TetO-Gfi1b

    Plasmid

    #131592

    Purpose

    doxycycline-inducible expression of mouse GFI1B in mammalian cells

    Depositor

    Kateri Moore, Filipe Pereira

    Article

    Pereira et al Cell Stem Cell. 2013 Aug 1;13(2):205-18.

    Insert

    Gfi1b (Gfi1b Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE-mCMV

    Available Since

    Dec. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. sgRNA BRCA2 V2102I

    Plasmid

    #139322

    Purpose

    Plasmid expressing a sgRNA to introduce BRCA2 V2102I using base editing

    Depositor

    Alberto Ciccia

    Article

    Billon et al Cell Rep. 2020 Mar 10;30(10):3280-3295.e

    Insert

    sgRNA to insert BRCA2 V2102I using base editing
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    May 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. sgRNA BRCA2 E2772K

    Plasmid

    #139323

    Purpose

    Plasmid expressing a sgRNA to introduce BRCA2 E2772K using base editing

    Depositor

    Alberto Ciccia

    Article

    Billon et al Cell Rep. 2020 Mar 10;30(10):3280-3295.e

    Insert

    sgRNA to insert BRCA2 E2772K using base editing
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    May 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.1-MUC2 D1D2D' with cleavable His tag

    Plasmid

    #187993

    Purpose

    Expresses MUC2 D1D2D' assembly with cleavable His tag in mammalian cells

    Depositor

    Deborah Fass

    Article

    Reznik et al Cell. 2022 Oct 27;185(22):4206-4215.e11.

    Insert

    Mucin2 D1D2D' (MUC2 Human)
    

    Use

    Tags

    6xHistadine followed by TEV cleavage site

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAWp100

    Plasmid

    #157977

    Purpose

    pBT264-mutmU6p-{2xBbsI}-sgRNA scaffold-{MfeI}

    Depositor

    Alan Siu Lun Wong

    Article

    Zhou et al Cell Rep. 2020 Aug 11;32(6):108020. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pGEMT-GDNF

    Plasmid

    #119897

    Purpose

    Mouse GDNF In situ hybridization probe

    Depositor

    Wenqin Luo

    Article

    Fleming et al Elife. 2015 Apr 2;4:e06828. doi: 10.7554

    Insert

    glial cell line derived neurotrophic factor (Gdnf Mouse)
    

    Use

    In situ hybridization probe

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. 49535-sgFMR1_E3B

    Plasmid

    #157783

    Purpose

    Expresses a sgRNA targeting the 3rd exon of human FMR1 gene

    Depositor

    Xinyu Zhao

    Article

    Li et al Genome Res. 2020 Mar;30(3):361-374. doi:

    Insert

    FMR1 (FMR1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. FB005

    Plasmid

    #119678

    Purpose

    Coding sequence nptII from E. coli domesticated into pUPD2 with AATG/GCTT barcodes. According to FungalBraid/GoldenBraid modular DNA assembly for A. tumefaciens-mediated genetic transformation

    Depositor

    Jose Marcos

    Article

    Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. d

    Insert

    geneticin resistance marker
    

    Use

    Synthetic Biology; Domestication of dna parts for…

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. BRCA1-(1316-1538)

    Plasmid

    #35079

    Depositor

    Cheryl Arrowsmith

    Article

    Mark et al J Mol Biol. 2005 Jan 14;345(2):275-87.

    Insert

    BRCA1 (BRCA1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains aa 1316-1538

    Promoter

    T7

    Available Since

    April 4, 2012

    Availability

    Academic Institutions and Nonprofits only

26. BRCA1-(498-663)

    Plasmid

    #35070

    Depositor

    Cheryl Arrowsmith

    Article

    Mark et al J Mol Biol. 2005 Jan 14;345(2):275-87.

    Insert

    BRCA1 (BRCA1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains aa 498-663

    Promoter

    T7

    Available Since

    April 6, 2012

    Availability

    Academic Institutions and Nonprofits only

27. sgRNA BRCA2 R2842C

    Plasmid

    #139324

    Purpose

    Plasmid expressing a sgRNA to introduce BRCA2 R2842C using base editing

    Depositor

    Alberto Ciccia

    Article

    Billon et al Cell Rep. 2020 Mar 10;30(10):3280-3295.e

    Insert

    sgRNA to insert BRCA2 R2842C using base editing
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    May 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. tdEos-Farnesyl-5

    Plasmid

    #57613

    Purpose

    Localization: Plasma Membrane, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Michael Davidson

    Article

    Kanchanawong et al Nature. 2010 Nov 25;468(7323):580-4. doi

    Insert

    Farnesyl (HRAS Human)
    

    Use

    Tags

    tdEos

    Expression

    Mammalian

    Mutation

    encodes final 20 aa of NM_001130442.1

    Promoter

    CMV

    Available Since

    Jan. 16, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

29. FLAG-SENP2 C548A

    Plasmid

    #126592

    Purpose

    Expresses siRNA resistant SENP2 (catalytic dead) in mammalian cells, Dox inducible in TetR cell lines

    Depositor

    Joanna Morris

    Article

    Garvin et al Genes Dev. 2019 Mar 1;33(5-6):333-347. d

    Insert

    SENP2 (SENP2 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    C548A

    Promoter

    CMV

    Available Since

    Oct. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. BRCA1-(540-758)

    Plasmid

    #35071

    Depositor

    Cheryl Arrowsmith

    Article

    Mark et al J Mol Biol. 2005 Jan 14;345(2):275-87.

    Insert

    BRCA1 (BRCA1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains aa 540-758

    Promoter

    T7

    Available Since

    May 14, 2012

    Availability

    Academic Institutions and Nonprofits only

31. pCW57.1 tTA SDHA

    Plasmid

    #177976

    Purpose

    All-in-one dox-repressible expression of cDNA

    Depositor

    Jessica Spinelli, David Sabatini

    Article

    Spinelli et al Science. 2021 Dec 3;374(6572):1227-1237.

    Insert

    SDHA (SDHA Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Silent point mutations to SDHA sgRNA target

    Promoter

    Available Since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. BRCA1-(360-498)

    Plasmid

    #35068

    Depositor

    Cheryl Arrowsmith

    Article

    Mark et al J Mol Biol. 2005 Jan 14;345(2):275-87.

    Insert

    BRCA1 (BRCA1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains aa 498-360

    Promoter

    T7

    Available Since

    April 4, 2012

    Availability

    Academic Institutions and Nonprofits only

33. pAWp102-v2 scaffold

    Plasmid

    #157978

    Purpose

    pBT264-mutmU6p-{2xBbsI}-v2 sgRNA scaffold-{MfeI}

    Depositor

    Alan Siu Lun Wong

    Article

    Zhou et al Cell Rep. 2020 Aug 11;32(6):108020. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pAWp40

    Plasmid

    #157980

    Purpose

    pFUGW-EFSp-BFP-{SbfI}

    Depositor

    Alan Siu Lun Wong

    Article

    Zhou et al Cell Rep. 2020 Aug 11;32(6):108020. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. 49535-sgFMR1_E17A

    Plasmid

    #157782

    Purpose

    Expresses a sgRNA targeting stop codon of human FMR1 gene

    Depositor

    Xinyu Zhao

    Article

    Li et al Genome Res. 2020 Mar;30(3):361-374. doi:

    Insert

    FMR1 (FMR1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. BRCA1-(758-1057)

    Plasmid

    #35085

    Depositor

    Cheryl Arrowsmith

    Article

    Mark et al J Mol Biol. 2005 Jan 14;345(2):275-87.

    Insert

    BRCA1 (BRCA1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains aa 758-1057

    Promoter

    T7

    Available Since

    April 4, 2012

    Availability

    Academic Institutions and Nonprofits only

37. FLAG-SENP2 WT

    Plasmid

    #126591

    Purpose

    Expresses siRNA resistant SENP2 (WT) in mammalian cells, Dox inducible in TetR cell lines

    Depositor

    Joanna Morris

    Article

    Garvin et al Genes Dev. 2019 Mar 1;33(5-6):333-347. d

    Insert

    SENP2 (SENP2 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    siRNA resistant to GAGGAGAUAUUCAGACAUU

    Promoter

    CMV

    Available Since

    Oct. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. p3E-Clta

    Plasmid

    #109585

    Purpose

    Multisite gateway vector for 3' tagging with Clathrin light chain a

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    Clathrin light chain a (clta Zebrafish)
    

    Use

    Zebrafish plasmids

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. BRCA1-(663-894)

    Plasmid

    #35072

    Depositor

    Cheryl Arrowsmith

    Article

    Mark et al J Mol Biol. 2005 Jan 14;345(2):275-87.

    Insert

    BRCA1 (BRCA1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains aa 663-894

    Promoter

    T7

    Available Since

    May 14, 2012

    Availability

    Academic Institutions and Nonprofits only

40. BRCA1-(758-936)

    Plasmid

    #35073

    Depositor

    Cheryl Arrowsmith

    Article

    Mark et al J Mol Biol. 2005 Jan 14;345(2):275-87.

    Insert

    BRCA1 (BRCA1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains aa 758-936

    Promoter

    T7

    Available Since

    April 6, 2012

    Availability

    Academic Institutions and Nonprofits only

41. BRCA1-(408-540)

    Plasmid

    #35069

    Depositor

    Cheryl Arrowsmith

    Article

    Mark et al J Mol Biol. 2005 Jan 14;345(2):275-87.

    Insert

    BRCA1 (BRCA1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains aa 408-540

    Promoter

    T7

    Available Since

    April 4, 2012

    Availability

    Academic Institutions and Nonprofits only

42. BRCA1-(219-758)

    Plasmid

    #35081

    Depositor

    Cheryl Arrowsmith

    Article

    Mark et al J Mol Biol. 2005 Jan 14;345(2):275-87.

    Insert

    BRCA1 (BRCA1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains aa 219-758

    Promoter

    T7

    Available Since

    April 4, 2012

    Availability

    Academic Institutions and Nonprofits only

43. BRCA1-(1443-1649)

    Plasmid

    #35080

    Depositor

    Cheryl Arrowsmith

    Article

    Mark et al J Mol Biol. 2005 Jan 14;345(2):275-87.

    Insert

    BRCA1 (BRCA1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Contains aa 1443-1649

    Promoter

    T7

    Available Since

    April 4, 2012

    Availability

    Academic Institutions and Nonprofits only

44. pUDR211

    Plasmid

    #113870

    Purpose

    expression of Cas9 programming sgRNA3 and sgRNA4 targetting HXT8 and HXT1 respectively

    Depositor

    Jack Pronk, Jean-Marc Daran

    Article

    Wijsman et al FEMS Yeast Res. 2019 Jan 1;19(1). pii: 5

    Insert

    sgRNA3-HXT8 / sgRNA4-HXT14
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Sept. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pUDR418

    Plasmid

    #113875

    Purpose

    expression of double Cas9 programming sgRNA11 targetting STL1

    Depositor

    Jack Pronk, Jean-Marc Daran

    Article

    Wijsman et al FEMS Yeast Res. 2019 Jan 1;19(1). pii: 5

    Insert

    doble sgRNA11-STL1
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Sept. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pSP64-hERG1a F656A

    Plasmid

    #53054

    Purpose

    Expresses alpha subunit of F656A hERG1a K channel in Xenopus oocytes

    Depositor

    Michael Sanguinetti

    Article

    Mitcheson et al Proc Natl Acad Sci U S A. 2000 Oct 24;97

    Insert

    Potassium voltage-gated channel subfamily H member 2 (KCNH2 Human)
    

    Use

    Xenopus oocyte expression

    Tags

    Expression

    Mutation

    changed Phenylalanine 656 to Alanine

    Promoter

    SP6

    Available Since

    June 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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47. pSP64-hERG1a Y652A

    Plasmid

    #53053

    Purpose

    Expresses alpha subunit of Y652A hERG1a K channel in Xenopus oocytes

    Depositor

    Michael Sanguinetti

    Article

    Mitcheson et al Proc Natl Acad Sci U S A. 2000 Oct 24;97

    Insert

    Potassium voltage-gated channel subfamily H member 2 (KCNH2 Human)
    

    Use

    Xenopus oocyte expression

    Tags

    Expression

    Mutation

    changed Tyrosine 652 to Alanine

    Promoter

    SP6

    Available Since

    June 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pLenti-BE3-PGK-Puro

    Plasmid

    #110842

    Purpose

    Lentiviral vector for constitutive expression of BE3 (not codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    BE3
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    D10A

    Promoter

    EF1s

    Available Since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pLenti-BE3-P2A-Puro

    Plasmid

    #110838

    Purpose

    Lentiviral vector for constitutive expression of BE3 (not codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    BE3
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    D10A

    Promoter

    EF1s

    Available Since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pCMV-BE3-FNLS

    Plasmid

    #110836

    Purpose

    CMV expression vector for BE3-FNLS construct (not codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    BE3-FNLS
    

    Use

    Tags

    3X FLAG

    Expression

    Mammalian

    Mutation

    NLS sequence at the N-terminus

    Promoter

    CMV

    Available Since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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