We narrowed to 5,311 results for: cycs

1. tetO-ESRRG

   Plasmid

   #170684

   Purpose

   doxycycline-inducible overexpression of ESRRG

   Depositor

   Paul Burridge

   Article

   Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

   Insert

   ESRRG (ESRRG Human)
   

   Use

   Lentiviral; Doxycycline inducible

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE promoter, Tet-ON

   Available since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. natMX-ins7

   Plasmid

   #195044

   Purpose

   pFA6a derived selection cassette 5' flanked with tCYC1 transcription terminator, allows genome modification without disruption of insertion neighboring genes by transcription interference

   Depositor

   Gloria Brar

   Article

   Powers et al Elife. 2022 Dec 12;11:e81086. doi: 10.75

   Insert

   NatR
   

   Use

   Yeast genomic targeting

   Tags

   tCYC1 S. cerevisiae, pPGK1 C. glabrata, NatR, tPG…

   Expression

   Mutation

   Promoter

   Available since

   Feb. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. psiCHECK2-E2F1 3'UTR

   Plasmid

   #29468

   Depositor

   Judy Lieberman

   Article

   Lal et al Mol Cell. 2009 Sep 11. 35(5):610-25.

   Insert

   E2F1 3'UTR (E2F1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 27, 2011

   Availability

   Academic Institutions and Nonprofits only

4. psiCHECK2-p27Kip1 3'UTR

   Plasmid

   #29476

   Depositor

   Judy Lieberman

   Article

   Lal et al Mol Cell. 2009 Sep 11. 35(5):610-25.

   Insert

   p27 Kip1 3'UTR (CDKN1B Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 27, 2011

   Availability

   Academic Institutions and Nonprofits only

5. FUW TetO HA-AMPKa1 AS

   Plasmid

   #69825

   Purpose

   Expresses AMPK alpha 1 in mammalian cells in a doxycycline inducible manner

   Depositor

   Anne Brunet

   Article

   Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

   Insert

   5'-AMP-activated protein kinase catalytic subunit alpha-1 (PRKAA1 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   minimal CMV promoter with tetracycline operator

   Available since

   May 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSICO-CPSF6-iRFP670

   Plasmid

   #167586

   Purpose

   Expresses human CPSF6 fused to iRFP

   Depositor

   Zandrea Ambrose

   Article

   Zhong et al mBio. 2021 Mar 23;12(2). pii: mBio.03142

   Insert

   CPSF6-iRFP670 (CPSF6 Human)
   

   Use

   Lentiviral

   Tags

   iRFP670

   Expression

   Mutation

   WT

   Promoter

   Available since

   April 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. MSCV-Puro-FH-AGO2-WT

   Plasmid

   #91986

   Purpose

   Expresses FLAG-HA-AGO2 (WT)

   Depositor

   Joshua Mendell

   Article

   Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

   Insert

   AGO2 (AGO2 Human)
   

   Use

   Retroviral

   Tags

   FLAG-HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEGFP-LC3-K51A

   Plasmid

   #155265

   Purpose

   Mammalian expression of rat LC3 fused to EGFP

   Depositor

   David Rubinsztein

   Article

   Puri et al Dev Cell. 2020 Apr 20;53(2):154-168.e6.

   Insert

   microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
   

   Use

   Tags

   pEGFP

   Expression

   Mammalian

   Mutation

   Lysine 51 to Alanine

   Promoter

   Available since

   May 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEGFP-LC3-R70A

   Plasmid

   #155264

   Purpose

   Mammalian expression of rat LC3 fused to EGFP

   Depositor

   David Rubinsztein

   Article

   Puri et al Dev Cell. 2020 Apr 20;53(2):154-168.e6.

   Insert

   microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
   

   Use

   Tags

   pEGFP

   Expression

   Mammalian

   Mutation

   Arginine 70 to Alanine

   Promoter

   Available since

   May 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. psiCHECK2-E2F3 3'UTR

    Plasmid

    #29470

    Depositor

    Judy Lieberman

    Article

    Lal et al Mol Cell. 2009 Sep 11. 35(5):610-25.

    Insert

    E2F3 3'UTR (E2F3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 27, 2011

    Availability

    Academic Institutions and Nonprofits only

11. psiCHECK2-CHEK1 3'UTR

    Plasmid

    #29478

    Depositor

    Judy Lieberman

    Article

    Lal et al Mol Cell. 2009 Sep 11. 35(5):610-25.

    Insert

    CHEK1 3'UTR (CHEK1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 27, 2011

    Availability

    Academic Institutions and Nonprofits only

12. 1068 pGL3 FasL promoter (no SV40 prom)

    Plasmid

    #9029

    Depositor

    William Sellers

    Article

    Nakamura et al Mol Cell Biol. 2000 Dec . 20(23):8969-82

    Insert

    Fas ligand promoter (FASLG Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Removed the SV40 promoter from pGL3

    Promoter

    Available since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

13. psiCHECK2-E2F2 3'UTR

    Plasmid

    #29469

    Depositor

    Judy Lieberman

    Article

    Lal et al Mol Cell. 2009 Sep 11. 35(5):610-25.

    Insert

    E2F2 3'UTR (E2F2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 27, 2011

    Availability

    Academic Institutions and Nonprofits only

14. pMP108 Nanog-Venus-2a-mCherry

    Plasmid

    #159743

    Purpose

    Nanog targeting vector to insert fluorescent reporter of protein and gene expression levels from endogenous locus in mouse embryonic stem cells. Please see depositor comments for more detail.

    Depositor

    Sergi Regot

    Article

    Pokrass et al Dev Cell. 2020 Nov 9;55(3):328-340.e5. d

    Insert

    Nanog homeobox (Nanog Mouse)
    

    Use

    Mouse Targeting

    Tags

    Venus-2a-mCherry

    Expression

    Mutation

    Promoter

    Available since

    July 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCIneo-AR-S81E

    Plasmid

    #171224

    Purpose

    Mammalian expression of un-tagged human androgen receptor phosphorylation mutant: AR-Ser81Glu (AR-S81E)

    Depositor

    Steven Balk

    Article

    Chen et al Proc Natl Acad Sci U S A. 2006 Oct 24;10

    Insert

    human androgen receptor Ser81Glu mutant (AR Human)
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    AR-Ser81Glu (AR-S81E)

    Promoter

    CMV

    Available since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. RCAS-sgATG7

    Plasmid

    #75228

    Purpose

    An adaption on the RCAS/tv-a somatic cell gene transfer system, for use in combination with an existing Cas9 background in the cell/mouse of interest. Depositors: Jane Fraser/Noor Gammoh

    Depositor

    Noor Gammoh

    Article

    Fraser et al EMBO Rep. 2019 Oct 4;20(10):e47734. doi:

    Insert

    Autophagy Related 7 (Atg7 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    June 2, 2016

    Availability

    Academic Institutions and Nonprofits only

17. FUW TetO NET1A-V5 WT

    Plasmid

    #69828

    Purpose

    Expresses NET1A-V5 in mammalian cells in a doxycycline inducible manner

    Depositor

    Anne Brunet

    Article

    Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

    Insert

    Neuroepithelial cell-transforming gene 1 protein A (NET1 Human)
    

    Use

    Lentiviral

    Tags

    v5

    Expression

    Mammalian

    Mutation

    Promoter

    minimal CMV promoter with tetracycline operator

    Available since

    April 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. MSCV-Puro-FH-AGO2-S831E

    Plasmid

    #91997

    Purpose

    Expresses FLAG-HA-AGO2 (S831E)

    Depositor

    Joshua Mendell

    Article

    Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

    Insert

    AGO2 (AGO2 Human)
    

    Use

    Retroviral

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    S831E

    Promoter

    Available since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. px458_2A_GFP_sgRNA_CELF2

    Plasmid

    #106102

    Purpose

    CRISPR knockout. Expresses Cas9, EGFP, and sgRNA targeting CELF2

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

    Insert

    gRNA targeting CELF2 (CELF2 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLJM1-FH-AGO2-S828A

    Plasmid

    #91982

    Purpose

    Expresses FLAG-HA-AGO2 (S828A)

    Depositor

    Joshua Mendell

    Article

    Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

    Insert

    AGO2 (AGO2 Human)
    

    Use

    Lentiviral

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    S828A

    Promoter

    Available since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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