We narrowed to 2,603 results for: ubiquitin

1. Fgf4enhLV (FpG12)

   Plasmid

   #69444

   Purpose

   positive control plasmid for lentiviral reporter expression in ESCs

   Depositor

   Lisa Dailey

   Article

   Murtha et al Nat Methods. 2014 May;11(5):559-65. doi:

   Inserts

   minimal FGF4 promoter
   EGFP
   Ubiquitin promoter
   Hygromycin resistance
   FGF4 enhancer (Fgf4 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEX-6P-1-HRD1 aa 236-444

   Plasmid

   #185349

   Purpose

   RING domain-containing GST fusion protein for ubiquitination assays.

   Depositor

   Allan Weissman

   Article

   Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

   Insert

   HRD1/SYVN (SYVN1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   aa 236-444 only

   Promoter

   Available since

   July 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-UBC-S1-jRCaMP1a

   Plasmid

   #160728

   Purpose

   Signaling reporter island (SiRI) construct for spatially multiplexed imaging. Contains RFP-based fluorescent reporter jRCaMP1a (calcium indicator), Xpress tag, and S1 protein scaffold.

   Depositor

   Edward Boyden

   Article

   Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31

   Insert

   S1-jRCaMP1a
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   Human ubiquitin C (UBC) promoter

   Available since

   Dec. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pZE21/UBP1/ClpS_V65I

   Plasmid

   #98567

   Purpose

   Expresses truncated codon-opt S. cerevisiae UBP1 and the V65I engineered variant of E. coli ClpS in an artificial operon

   Depositor

   George Church

   Article

   Kunjapur et al Proc Natl Acad Sci U S A. 2018 Jan 16;11

   Inserts

   Truncated ubiquitin cleavase, codon optimized
   Mutated ATP-dependent Clp protease adapter protein
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Contains the V65I mutation that increases discrim…

   Promoter

   Available since

   Feb. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEX-6P-1-gp78 RING

   Plasmid

   #185353

   Purpose

   RING domain-containing GST fusion protein for ubiquitination assays.

   Depositor

   Allan Weissman

   Article

   Smith et al PLoS Biol. 2021 Dec 8;19(12):e3001474. d

   Insert

   gp78 cytoplasmic domain (AMFR Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Stop codon after aa 393

   Promoter

   Available since

   Oct. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. MSCVhyg-HA-FLAG_Ubc9_C93A

   Plasmid

   #164937

   Purpose

   Expresses FLAG & HA tagged human Ubiquitin Conjugating Enzyme 9 (UBC9) _C93A mutant cDNA

   Depositor

   Ji Luo

   Article

   Yu et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

   Insert

   human UBC9 (UBE2I Human)
   

   Use

   Retroviral

   Tags

   FLAG & HA tags

   Expression

   Mutation

   Promoter

   PGK promoter

   Available since

   May 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCUU

   Plasmid

   #159747

   Purpose

   Ubiquitin promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter). Selection of transgenic seeds by CFP fluoresce.

   Depositor

   Wolfgang Lukowitz

   Article

   Angulo et al bioRxiv

   Insert

   gRNA scaffold
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Oct. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSLfa-PUb-MCS

   Plasmid

   #52908

   Purpose

   Aedes aegypti polyubiquitin promoter followed by MCS

   Depositor

   Zach Adelman

   Type

   Empty backbone

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Ae aegptyi polyubiquitin

   Available since

   June 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. PSL1180polyUBdsRED

   Plasmid

   #49327

   Purpose

   Donor plasmid for homologous recombination that expresses dsRED constitutively using the Aedes aegypti polyubiquitin (PUb) promoter.

   Depositor

   Leslie Vosshall

   Insert

   dsRED
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Aedes polyubiquitin

   Available since

   Nov. 18, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSL1180-HR-PUbECFP

    Plasmid

    #47917

    Purpose

    Donor plasmid for homologous recombination that expresses ECFP consitutively using the Aedes aegypti polyubiquitin (PUb) promoter.

    Depositor

    Leslie Vosshall

    Insert

    ECFP
    

    Use

    Tags

    NLS

    Expression

    Insect

    Mutation

    Promoter

    Aedes aegypti polyubiquitin (PUb)

    Available since

    Sept. 26, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. Flag-HA-GFP

    Plasmid

    #22612

    Depositor

    Wade Harper

    Article

    Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

    Insert

    GFP
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    Dec. 4, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCLXSN-GFP

    Plasmid

    #27557

    Depositor

    Shao-Cong Sun

    Article

    Reiley et al Mol Cell Biol. 2005 May . 25(10):3886-95

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. MSCV-N-Flag-HA-IRES-PURO

    Plasmid

    #41033

    Depositor

    Wade Harper

    Article

    Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

    Type

    Empty backbone

    Use

    Retroviral; Gateway destination vector

    Tags

    Flag, HA, and IRES Puro

    Expression

    Mammalian

    Mutation

    Promoter

    LTR-driven expression

    Available since

    Oct. 26, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pST39-Ubc13-MMS2

    Plasmid

    #184807

    Purpose

    Bacterial expression of yeast UEV-E2 complex

    Depositor

    James Hurley

    Article

    Ren et al EMBO J. 2010 Mar 17;29(6):1045-54. Epub

    Insert

    Ubc13, MMS2
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. Flag-HA-LOC220594

    Plasmid

    #22562

    Depositor

    Wade Harper

    Article

    Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

    Insert

    LOC220594 (USP32P2 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGST2-yHse1-VHS

    Plasmid

    #44415

    Depositor

    James Hurley

    Article

    Ren et al EMBO J. 2010 Mar 17;29(6):1045-54. Epub

    Insert

    Hse1-VHS (HSE1 Budding Yeast)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 29, 2013

    Availability

    Academic Institutions and Nonprofits only

17. Sus1/PRSF

    Plasmid

    #26961

    Depositor

    Cynthia Wolberger

    Article

    Samara et al Science. 2010 May 21. 328(5981):1025-9.

    Insert

    Sus1 (SUS1 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

18. Sgf11/pCDF

    Plasmid

    #26960

    Depositor

    Cynthia Wolberger

    Article

    Samara et al Science. 2010 May 21. 328(5981):1025-9.

    Insert

    sgf11 (SGF11 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 4, 2011

    Availability

    Academic Institutions and Nonprofits only

19. Flag-HA-OTUD5

    Plasmid

    #22610

    Depositor

    Wade Harper

    Article

    Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

    Insert

    OTUD5 (OTUD5 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/TO

    Available since

    Nov. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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20. Flag-HA-OTUD7B

    Plasmid

    #22550

    Depositor

    Wade Harper

    Article

    Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

    Insert

    OTUD7B (OTUD7B Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    Nov. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge