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pAAV-hSyn1-mRuby2-GSG-P2A-GCaMP6m-WPRE-pA

Plasmid

#51473

Purpose

Bicistronic vector expressing mRuby2 and GCaMP6m from a single open reading frame.

Depositor

Tobias Bonhoeffer, Mark Huebener, Tobias Rose

Article

Rose et al Science. 2016 Jun 10;352(6291):1319-22.

Insert

mRuby2-P2A-GCaMP6m

Use

AAV

Promoter

hSyn1

Available Since

April 16, 2014

Availability

Academic Institutions and Nonprofits only

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pRRL-VinculinCS

Plasmid

#200309

Purpose

Lentivirus vector expressing vinculin with FRET conformational sensor

Depositor

Brenton Hoffman

Article

Tao et al Dev Cell. 2023 Mar 10:S1534-5807(23)0007

Insert

VinculinCS

Use

Lentiviral

Promoter

T7

Available Since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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tdTomato-Paxillin-22

Plasmid

#58123

Purpose

Localization: Focal Adhesions, Excitation: 554, Emission: 581

Depositor

Michael Davidson

Insert

Paxillin

Tags

tdTomato

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 19, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-Y-RAN-GFP26

Plasmid

#106423

Purpose

Y-RAN-GFP26 (RANbody GFP26::Chicken IgY)

Depositor

Joshua Sanes

Article

Yamagata et al Proc Natl Acad Sci U S A. 2018 Feb 13. p

Insert

Y-RAN-GFP26

Tags

HA and His

Expression

Mammalian

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Vinculin_TurboID_Venus

Plasmid

#200272

Purpose

Expressing vinculin with co-localization labeler TurboID and fluorescence reporter Venus

Depositor

Brenton Hoffman

Article

Tao et al Dev Cell. 2023 Mar 10:S1534-5807(23)0007

Insert

Vinculin_TurboID_Venus

Expression

Mammalian

Promoter

T7

Available Since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pMK285 (Stag-3FLAG-Bsr)

Plasmid

#72801

Purpose

C-terminal tagging with Stag-3FLAG using CRIPSR/Cas9

Depositor

Masato Kanemaki

Article

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Insert

Stag-3FLAG-Bsr

Use

CRISPR

Tags

Stag-3FLAG

Expression

Mammalian

Available Since

March 24, 2016

Availability

Academic Institutions and Nonprofits only

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pRRL-VinculinTS-A50I

Plasmid

#111831

Purpose

Vinculin tension sensor with point mutation affecting ability to bind talin in lentiviral expression vector

Depositor

Brenton Hoffman

Article

Rothenberg et al Biophys J. 2018 Apr 10;114(7):1680-1694.

Insert

VinculinTS (A50I) (VCL Chicken)

Use

Lentiviral

Mutation

changed vinculin alanine 50 to isoleucine

Promoter

CMV

Available Since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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VinTS-tCRMod-GGSGGS5

Plasmid

#111763

Purpose

Expresses vinculin tension sensor comprised of Clover-(GGSGGS)5-mRuby2 module

Depositor

Brenton Hoffman

Article

LaCroix et al eLife. 2018 Jul 19;7. pii: 33927. doi: 1

Insert

Clover-mRuby2-based vinculin tension sensor with (GGSGGS)5 extensible domain

Expression

Mammalian

Mutation

Residues 1-228 of Clover, residues 1-3 deleted fr…

Promoter

CMV

Available Since

July 5, 2018

Availability

Academic Institutions and Nonprofits only

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mEmerald-FAK-5

Plasmid

#54091

Purpose

Localization: Focal Adhesion Kinase, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Insert

FAK

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

July 2, 2014

Availability

Industry, Academic Institutions, and Nonprofits
mApple-Paxillin-22

Plasmid

#54935

Purpose

Localization: Focal Adhesions, Excitation: 568, Emission: 592

Depositor

Michael Davidson

Article

Thievessen et al J Cell Biol. 2013 Jul 8;202(1):163-77. d

Insert

Paxillin

Tags

mApple

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 7, 2014

Availability

Academic Institutions and Nonprofits only
pZac2.1-GfaABC1D-lck-jGCaMP8m

Plasmid

#176760

Purpose

AAV-mediated expression of membrane bound GCaMP8m under the control of GfaABC1D promoter

Depositor

Loren Looger

Has Service

AAV1, AAV5, and AAV9

Insert

lck-jGCaMP8m

Use

AAV

Expression

Mammalian

Promoter

GfaABC1D

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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2XDR4

Plasmid

#32615

Depositor

Connie Cepko

Article

Billings et al PLoS One. 2010 . 5(10):e13739.

Insert

2XDR4

Expression

Mammalian

Available Since

July 30, 2013

Availability

Academic Institutions and Nonprofits only

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AP-SEMA3A (chick)

Plasmid

#29448

Depositor

Jonathan Raper

Article

Feiner et al Neuron. 1997 Sep . 19(3):539-45.

Insert

AP-SEMA3A (chick) (SEMA3A Chicken)

Tags

AP tagged

Expression

Mammalian

Available Since

July 1, 2011

Availability

Academic Institutions and Nonprofits only
pGP-CMV-GCaMP3-373

Plasmid

#91779

Purpose

Mammalian expression of low affinity calcium-sensing fluorescent protein

Depositor

Douglas Kim, GENIE Project

Article

de Juan-Sanz et al Neuron. 2017 Jan 30. pii: S0896-6273(17)

Insert

GCaMP3-373

Tags

6xHis, T7 epitope, and Xpress tag

Expression

Mammalian

Promoter

CMV

Available Since

June 27, 2017

Availability

Academic Institutions and Nonprofits only

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mCh-LgBiT-2A-EGFP-Vin-PILATeS-CT

Plasmid

#216763

Purpose

PiggyBac vector for stable co-expression of mCherry-LgBiT and EGFP-Vinculin-PILATeS-CT (700 pM) negative control in mammalian cells. Requires transposase

Depositor

Alex Dunn

Article

Zhong et al ACS Sens. 2024 Jul 26;9(7):3489-3495. do

Insert

mCherry-LgBiT-P2A-T2A-EGFP-Vin-PILATeS-CT

Tags

EGFP and mCherry

Expression

Mammalian

Available Since

Aug. 13, 2024

Availability

Academic Institutions and Nonprofits only

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mEmerald-Paxillin-22

Plasmid

#54219

Purpose

Localization: Focal Adhesions, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Paszek et al Nat Methods. 2012 Jul 1;9(8):825-7. doi:

Insert

Paxillin

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

July 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pSNAP-CAPZ

Plasmid

#69948

Purpose

Simultaneous expression of a His- and SNAP-tagged β1 subunit and untagged α1 subunits of chicken CapZ

Depositor

Jeff Gelles

Article

Bombardier et al Nat Commun. 2015 Nov 13;6:8707. doi: 10.

Inserts

CapZ beta 1 (CAPZB Chicken)
CapZ alpha 1 (CAPZA1 Chicken)

Tags

His 6 and SNAP

Expression

Bacterial

Available Since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only

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aTRE

Plasmid

#32612

Depositor

Connie Cepko

Article

Billings et al PLoS One. 2010 . 5(10):e13739.

Insert

Thyroid Response Element

Expression

Mammalian

Available Since

Dec. 10, 2012

Availability

Academic Institutions and Nonprofits only

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pCDNA-FRT-FAK10

Plasmid

#59119

Purpose

FAK fret sensor with 10 nm helix

Depositor

Sivaraj Sivaramakrishnan

Article

Ritt et al J Biol Chem. 2013 Mar 29;288(13):8875-86

Insert

Focal Adhesion Kinase

Tags

FLAG, mCerulean, and mCitrine

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pET30b jGCaMP8f F366H

Plasmid

#204139

Purpose

Production of recombinant calcium sensor jGCaMP8f F366H high dynamic range variant in E.coli

Depositor

Katalin Torok

Article

Tran et al Front Cell Neurosci. 2023 May 18;17:1155

Insert

jGCaMP8f F366H

Tags

N-terminal His tag

Expression

Bacterial

Mutation

Phenylalanine 366 changed to histidine

Promoter

T7

Available Since

Dec. 8, 2023

Availability

Academic Institutions and Nonprofits only

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