58,349 results

1. pTRE3G-human cGAS

   Plasmid

   #186853

   Purpose

   Doxycycline-dependent expression of human cGAS gene in mammalian cells by retroviral transduction

   Depositor

   Jonathan Kagan

   Article

   Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

   Insert

   Human cGAS without tags (Adamts1 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   TRE3G

   Available since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSLIK DNM1L-V5 neo

   Plasmid

   #220363

   Purpose

   Lentiviral expression vector for an inducible V5-tagged DNM1L construct

   Depositor

   Kevin Janes

   Insert

   DNM1L (DNM1L Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. human KANK2 Ankyrin Repeats pET151

   Plasmid

   #164054

   Purpose

   Expresses human KANK2 Ank domain in bacterial cells

   Depositor

   Ben Goult

   Insert

   Kidney Ankyrin Repeat-Containing Protein 2 (KANK2 Human)
   

   Use

   Tags

   His-tag, TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. Crimson/P2A-Control (pSLIK3)

   Plasmid

   #113858

   Purpose

   An isogenic synaptase-free control vector to evaluate recombination using endogenous functions for N-terminal Crimson/P2A fusion plasmids.

   Depositor

   Richard S Myers

   Article

   Valledor et al PLoS One. 2018 Aug 15;13(8):e0200955. do

   Insert

   E2-Crimson
   

   Use

   Lentiviral

   Tags

   P2A/Crimson

   Expression

   Mutation

   Promoter

   Available since

   June 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. tFucci(CA)5

   Plasmid

   #153521

   Purpose

   New color variant of Fucci (Fluorescent ubiquitination-based cell cycle indicator) : Fucci(CA)5.

   Depositor

   Atsushi Miyawaki

   Article

   Ando et al Cell Struct Funct. 2023 Jun 30. doi: 10.

   Insert

   tFucci(CA)5.
   

   Use

   Tags

   AzaleaB5-hCdt1(1/100)Cy(-)_P2A_h2-3-hGem(1/110)

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Aug. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCEP4-M2L

   Plasmid

   #20926

   Purpose

   used in the derivation of human iPS cells using non-integrating episomal vectors; expresses cMyc and Lin28

   Depositor

   James Thomson

   Article

   Yu et al Science. 2009 Mar 26.

   Inserts

   MYC (MYC Human)
   LIN28 (LIN28A Human)
   

   Use

   Episomal/ebna

   Tags

   Expression

   Mutation

   Promoter

   CMV and IRES

   Available since

   May 20, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. GFP-Dcp1b

   Plasmid

   #153976

   Purpose

   Fluorescent protein fused to mRNA decapping enzyme 1b

   Depositor

   Gia Voeltz

   Article

   Lee et al Science. 2020 Jan 31;367(6477). pii: 367

   Insert

   mRNA recapping enzybe 1b (DCP1B Human)
   

   Use

   Tags

   pAcGFP-C1

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJRH-1328 LV EF1a-CD20 IRES-EGFP

   Plasmid

   #201918

   Purpose

   2nd generation lentiviral transfer plasmid for engineering cells to express human CD20

   Depositor

   Jennifer Doudna

   Article

   Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

   Insert

   CD20
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Aug. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEGFP-HDAC4

   Plasmid

   #45636

   Depositor

   Tso-Pang Yao

   Article

   Cohen et al J Biol Chem. 2007 Nov 16;282(46):33752-9

   Insert

   HDAC4 (HDAC4 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 25, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. phROSA26-Tet-HA-JSAP1_WT

    Plasmid

    #208772

    Purpose

    Tetracycline inducible expression vector for HA-JSAP1_WT, used as a donor plasmid for HA-JSAP1_WT knock-in at the human ROSA26 locus

    Depositor

    Katsuji Yoshioka

    Article

    Suzuki et al Genes Cells. 2023 Nov 14. doi: 10.1111/g

    Insert

    JSAP1 (MAPK8IP3 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pECFP_C1_NSD2 FL

    Plasmid

    #221382

    Purpose

    Expresses NSD2 in mammalian cells

    Depositor

    Albert Jeltsch

    Article

    Weirich et al Commun Biol. 2024 Jun 8;7(1):707. doi: 1

    Insert

    Histone-lysine N-methyltransferase NSD2 (NSD2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    L237R mutation in ECFP (Please see depositor comm…

    Promoter

    CMV

    Available since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. SNAP-Dcp2

    Plasmid

    #153975

    Purpose

    SNAP-tag polypeptide fused to mRNA decapping enzyme 2

    Depositor

    Gia Voeltz

    Article

    Lee et al Science. 2020 Jan 31;367(6477). pii: 367

    Insert

    mRNA Decapping enzyme 2 (DCP2 Human)
    

    Use

    Tags

    SNAP-C1

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. vYM019_PB-Blast-pEF1a-H2B-mCh-D3bCD

    Plasmid

    #197687

    Purpose

    plasmid for expressing the DNMT3bCD

    Depositor

    Michael Elowitz

    Article

    Ma et al ACS Synth Biol. 2023 Aug 12. doi: 10.102

    Insert

    H2B-mCherry-T2A-rTetR-DNMT3bCD (DNMT3B Human, Synthetic)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCIneo-V5-SBP-MBP-HsNot9shRNA2res_AG

    Plasmid

    #148828

    Purpose

    Mammalian Expression of HsNot9-shRNA2res

    Depositor

    Elisa Izaurralde

    Article

    Sgromo et al RNA. 2018 Mar;24(3):381-395. doi: 10.126

    Insert

    HsNot9-shRNA2res (CNOT9 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-hSyn1-FLEX-NEPLDCV-P2A-mRUBY3-WPRE

    Plasmid

    #207664

    Purpose

    Neuropeptide degradation

    Depositor

    Sung Han

    Article

    Kim et al bioRxiv 2023.01.19.524797

    Insert

    NEP (MME Human)
    

    Use

    AAV and Cre/Lox

    Tags

    mRUBY3

    Expression

    Mutation

    Addition of signaling peptide (POMC) on the N-ter…

    Promoter

    hSynapsin

    Available since

    Dec. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. OpenCRISPR-1 sgRNA-12nt stem

    Plasmid

    #221567

    Purpose

    Express OpenCRISPR-1 sgRNA (12nt stem loop) in human cells using a U6 promoter. Plasmid also encodes a CMV-driven GFP reporter.

    Depositor

    Ali Madani

    Article

    Ruffolo et al Nature. 2025 Jul 30. doi: 10.1038/s41586

    Insert

    OpenCRISPR-1 sgRNA 12nt stem loop
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 28, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pLentiRNACRISPR_007 - TetO-NLS-RfxCas13d-NLS-WPRE-EFS-rtTA3-2A-Blast

    Plasmid

    #138149

    Purpose

    Expresses RfxCas13d in mammalian cells for knockdown of target RNAs in combination with compatible crRNAs. Localized to the nucleus

    Depositor

    Neville Sanjana

    Article

    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

    Insert

    CasRx
    

    Use

    Lentiviral

    Tags

    NLS-HA

    Expression

    Mutation

    Promoter

    Available since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.2 TDP-43 YFP

    Plasmid

    #84911

    Purpose

    expression of TDP-43 YFP in mammalian cells

    Depositor

    Aaron Gitler

    Article

    Elden et al Nature. 2010 Aug 26;466(7310):1069-75. d

    Insert

    TDP-43 (TARDBP Human)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. FLX1.8NFATGFPpd2HS4

    Plasmid

    #169096

    Purpose

    Lentiviral vector encoding a short-lived GFP under the transcriptional control of a mutlimerized NFAT gene enhancer to measure T cell activation

    Depositor

    Geoffrey Owens

    Inserts

    NFAT enhancer (from IL-4 promoter)
    Destabilized green fluorescent protein
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    NFAT enhancer + synthetic TATA box

    Available since

    July 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. phMYT1L-N174

    Plasmid

    #66809

    Purpose

    2nd generation lentiviral transfer plasmid. Expresses human MYT1L with G418 resistance

    Depositor

    Andrew Yoo

    Article

    Richner et al Nat Protoc. 2015 Oct;10(10):1543-55. doi

    Insert

    MYT1L (MYT1L Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human EF1a

    Available since

    Sept. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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