2,226 results

1. pEGFP-N1-TrkB

   Plasmid

   #32500

   Depositor

   Rosalind Segal

   Insert

   TRKB (Ntrk2 Rat)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 13, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. CGBE1-NG (pBM1246)

   Plasmid

   #140256

   Purpose

   CMV promoter expression plasmid for UNG(E.coli)-rAPOBEC1(R33A)-nCas9_NG-P2A-EGFP

   Depositor

   Keith Joung

   Article

   Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

   Insert

   eUNG-BE4max(R33A)_NG-ΔUGI
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   R33A in rAPOBEC1, NG mutations in SpCas9, D10A in…

   Promoter

   CMV

   Available since

   Aug. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-SypHluorin 4x (S4x)

   Plasmid

   #37005

   Depositor

   Stephen Heinemann, Yongling Zhu

   Article

   Zhu et al Neuron. 2009 Feb 12;61(3):397-411.

   Insert

   SypHluorin 4x (Syp Rat)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Four copies of pHluorin inserted at site 184-185

   Promoter

   CMV

   Available since

   July 12, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. FLAG.PKCzeta.K/W

   Plasmid

   #10800

   Depositor

   Alex Toker

   Article

   Chou et al Curr Biol. 1998 Sep 24. 8(19):1069-77.

   Insert

   PKC zeta (Prkcz Rat)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Kinase dead mutant: K281W (Lys 281 to Trp)

   Promoter

   Available since

   Dec. 7, 2005

   Availability

   Academic Institutions and Nonprofits only

5. pET-Hisx6-rAPOBEC1-XTEN-nCas9-UGI-NLS

   Plasmid

   #89508

   Purpose

   Expression of Hisx6-rAPOBEC1-XTEN-nCas9-UGI-NLS in bacterial cells.

   Depositor

   Jin-Soo Kim

   Article

   Kim et al Nat Biotechnol. 2017 Feb 27. doi: 10.103

   Insert

   rAPOBEC1-nCas9 (D10A)-UGI-NLS
   

   Use

   Tags

   UGI and rAPOBEC-XTEN

   Expression

   Bacterial

   Mutation

   D10A mutation of Cas9

   Promoter

   T7

   Available since

   May 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pORANGE Gria1-GFP KI

   Plasmid

   #131489

   Purpose

   Endogenous tagging of GluA1: C-terminal (amino acid position: STOP codon)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and GFP donor (Gria1, Gria1 Mouse, Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and Cbh

   Available since

   Sept. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-Vamp3-pHluorin

   Plasmid

   #190152

   Purpose

   Expresses (pH-sensitive) super ecliptic pHluorin-tagged rat Vamp3 for imaging exocytosis

   Depositor

   Brad Zuchero

   Article

   Lam et al Nat Commun. 2022 Sep 23;13(1):5583. doi:

   Insert

   Vamp3 (Vamp3 Rat)
   

   Use

   AAV

   Tags

   superecliptic pHluorin

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. Rat Alpha2 delta-1 HA pMT2

   Plasmid

   #58729

   Purpose

   expression of HA tagged rat Alpha2 delta-1 in mamalian cells

   Depositor

   Annette Dolphin

   Article

   Kadurin et al J Biol Chem. 2012 Sep 28;287(40):33554-6

   Insert

   alpha2 delta-1 (Cacna2d1 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SV40

   Available since

   Sept. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-TRE-GCaMP6s

   Plasmid

   #135418

   Purpose

   Can be used to drive amplified GCaMP6s expression when combined with TET driver . Can be used to create adeno-associated virus to express TET-driven GCaMP6s.

   Depositor

   Rylan Larsen

   Insert

   GCaMP6s
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE

   Available since

   Feb. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pZac2.1-GfaABC1D-lck-jGCaMP8s

    Plasmid

    #176761

    Purpose

    AAV-mediated expression of membrane bound GCaMP8s under the control of GfaABC1D promoter

    Depositor

    Loren Looger

    Has Service

    AAV1 and AAV9

    Insert

    lck-jGCaMP8s
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    GfaABC1D

    Available since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. CMV-mEGFP(A206K)-paCaMKII(K42M)

    Plasmid

    #165440

    Purpose

    Expresses photoactivatable CaMKII(K42M: Kinase dead) in mammalian cells as a negative control.

    Depositor

    Hideji Murakoshi

    Article

    Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

    Insert

    mEGFP(A206K)-paCaMKII(K42M)
    

    Use

    Tags

    mEGFP(A206K)

    Expression

    Mammalian

    Mutation

    K42M: Kinase dead mutation

    Promoter

    CMV

    Available since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDC180

    Plasmid

    #218673

    Purpose

    Expresses rat ⍺SNAP in bacteria

    Depositor

    Axel Brunger

    Article

    Leitz et al Nat Protoc. 2024 Jul 2. doi: 10.1038/s41

    Insert

    rat ⍺SNAP (Napa Rat)
    

    Use

    Tags

    6 his

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. Kif5A-GFP-CIBN

    Plasmid

    #102252

    Purpose

    Expresses fusion of kinesin heavy chain 5A (1-572) with GFP and CIB1 (1-170)

    Depositor

    Bianxiao Cui

    Article

    Duan et al Chem Biol. 2015 May 21;22(5):671-82. doi

    Insert

    Kif5A-GFP-CIB1 (Kif5a, CIB1 Rat, Mustard Weed)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGEX-6p1-GST-ArcFL

    Plasmid

    #119877

    Purpose

    Bacterial Expression construct for protein production and purification

    Depositor

    Jason Shepherd

    Article

    Pastuzyn et al Cell. 2018 Mar 22;173(1):275. doi: 10.10

    Insert

    Activity Regulated Cytoskeletal Protein (Arc Rat)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    March 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAV-hSyn-RiboL1-jGCaMP8f

    Plasmid

    #169249

    Purpose

    AAV transfer plasmid for neuronal expression of soma-targeted (RL-10, ribosomal tag) jGCaMP8f, with a flexible GS-linker sequence attaching the ribosomal tag to the jGCaMP8f protein.

    Depositor

    Marianne Fyhn

    Article

    Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

    Insert

    RiboL1-jGCaMP8f
    

    Use

    AAV

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    Dec. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-CIB1-mCerulean-MP

    Plasmid

    #58366

    Purpose

    For use in light-induced protein inactivation through the interaction with CRY2

    Depositor

    Won Do Heo

    Article

    Lee et al Nat Methods. 2014 Jun;11(6):633-6. doi:

    Inserts

    CIB1[1-147] (CIB1 Mustard Weed)
    Camk2a[315-478] (Camk2a Rat)
    

    Use

    Tags

    mCerulean

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 30, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-synp-F-synaptophysin-jRGECO1a

    Plasmid

    #102997

    Purpose

    Expresses synaptophysin fused jRGECO1a under the synapsin promoter

    Depositor

    David Cox

    Insert

    Synaptophysin-jRGECO1a
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMA3379

    Plasmid

    #46874

    Purpose

    A lentiviral vector for the doxycycline-inducible expression of soluble guanylate cyclase1 alpha3

    Depositor

    Mikhail Alexeyev, Troy Stevens

    Article

    Ochoa et al J Biol Chem. 2012 Jul 20;287(30):25407-1

    Insert

    soluble guanylate cyclase 1alpha3 (Gucy1a3 Rat)
    

    Use

    Lentiviral

    Tags

    Myc

    Expression

    Mutation

    Promoter

    TRE-Tight

    Available since

    Dec. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSEP-LAMP1-RFP

    Plasmid

    #167970

    Purpose

    Expresses the chimeric construct SEP-LAMP1-RFP where superecliptic pHluorin (SEP) is fused to the luminal domain of LAMP1-RFP

    Depositor

    Juan Bonifacino

    Article

    Farias et al Proc Natl Acad Sci U S A. 2017 Apr 4;114

    Insert

    LAMP1 (Lamp1 Rat)
    

    Use

    Tags

    SEP and mRFP

    Expression

    Mammalian

    Mutation

    D50E

    Promoter

    CMV

    Available since

    April 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLV-MPAct-mRuby3-IRES-Puro

    Plasmid

    #155231

    Purpose

    lentiviral MPAct construct

    Depositor

    Tobias Meyer

    Article

    Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

    Insert

    Lentiviral expression of MPAct-mRuby sensor
    

    Use

    Lentiviral

    Tags

    mRuby3

    Expression

    Mutation

    Promoter

    Available since

    Aug. 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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21. pAAV2.5-THP-GFP/WGA

    Plasmid

    #80337

    Purpose

    Fluorescent Reporter for Dopaminergic Neuron Differentiation

    Depositor

    Kwang-Soo Kim

    Article

    Oh et al Gene Ther. 2009 Mar;16(3):437-40. doi: 1

    Insert

    rat TH promoter (Th Rat)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    rat Tyrosine hydroxylase

    Available since

    Oct. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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22. pCI-SEP-GluR2(Q)

    Plasmid

    #24002

    Depositor

    Robert Malinow

    Article

    Kopec et al J Neurosci. 2006 Feb 15. 26(7):2000-9.

    Insert

    GluR2 (Gria2 Rat)
    

    Use

    Tags

    GluR2 signal peptide (aa1-24) and SEP (supereclip…

    Expression

    Mammalian

    Mutation

    R607Q

    Promoter

    CMV

    Available since

    April 6, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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23. pCLC3sGFP

    Plasmid

    #52423

    Purpose

    mammalian expression of rat CLC3 short transcript fused to EGFP

    Depositor

    Steven Weinman

    Article

    Li et al Am J Physiol Cell Physiol. 2002 Jun;282(

    Insert

    CLC3 (Clcn3 Rat)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAAV.GFAP.iGluSnFR3.v857.PDGFR

    Plasmid

    #178336

    Purpose

    Fluorescent reporter for glutamate, third generation, variant 857. iGluSnFR3.v857

    Depositor

    Kaspar Podgorski

    Article

    Aggarwal et al Nat Methods. 2023 May 4. doi: 10.1038/s4

    Insert

    pAAV GFAP iGluSnFR3 v857.PDGFR
    

    Use

    AAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…

    Tags

    IgK-chain, Myc epi tag, and PDGFR TM Domain

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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25. pcDNA3-rat PKA-RIIbeta-mEGFP

    Plasmid

    #45523

    Depositor

    Haining Zhong

    Article

    Zhong et al Neuron. 2009 May 14;62(3):363-74. doi: 1

    Insert

    PKA regulatory subunit RII beta tagged by mEGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 13, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pEGFP-N3 alpha-SSeCKS

    Plasmid

    #61294

    Purpose

    Mammalian expression of GFP-alpha-SSeCKS

    Depositor

    Irwin Gelman

    Insert

    alpha SSeCKS (Akap12 Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 9, 2014

    Availability

    Academic Institutions and Nonprofits only

27. pHR-mCherry-P2A-strepII-PPPCB

    Plasmid

    #67923

    Purpose

    Expresses mCherry and strepII-tag-PP2aBeta in mammalian cells

    Depositor

    Ron Vale

    Article

    Tanenbaum et al Elife. 2015 Aug 25;4. doi: 10.7554/eLife

    Insert

    mCherry-P2A-StrepII-PP2aBeta (Ppp2cb Rat)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. TLCV2-APOBEC1-YTHmut

    Plasmid

    #178950

    Purpose

    Lentiviral vector for dox-inducible expression of APOBEC1-YTHmut in mammalian cells

    Depositor

    Kate Meyer

    Article

    Tegowski et al Mol Cell. 2022 Feb 17;82(4):868-878.e10.

    Insert

    APOBEC1-YTHmut-T2A-GFP
    

    Use

    Lentiviral and Synthetic Biology; Inducible

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    tight TRE

    Available since

    April 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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29. VAMP2-pHuji

    Plasmid

    #105289

    Purpose

    VAMP2 tagged with pH-sensitive red fluorescent protein pHuji

    Depositor

    Justin Taraska

    Article

    Martineau et al Nat Commun. 2017 Nov 10;8(1):1412. doi:

    Insert

    VAMP2 (Vamp2 Rat)
    

    Use

    Tags

    pHuji

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Shank3-HA/pRK5

    Plasmid

    #197335

    Purpose

    Plasmid expressing an antigen targeting Shank3

    Depositor

    Morgan Sheng

    Insert

    Shank3 (Shank3 Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge