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pRSET-6xTR-TUBE

Plasmid

#110313

Purpose

E. coli expression and purification of 6xTR-TUBE

Depositor

Yasushi Saeki

Article

Tsuchiya et al Nat Commun. 2018 Feb 6;9(1):524. doi: 10

Insert

6xTR-TUBE (UBQLN1 Human)

Tags

N-terminal His6-T7 tag

Expression

Bacterial

Mutation

All Arg residues in the UBA domain are mutated to…

Promoter

T7

Available Since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

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mNeonGreen-Giantin

Plasmid

#98880

Purpose

In vivo visualization of the Golgi apparatus (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

Giantin

Tags

mNeonGreen

Expression

Mammalian

Promoter

CMV

Available Since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

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YAP1 (S6A) - V5 in pLX304

Plasmid

#42562

Depositor

William Hahn

Article

Rosenbluh et al Cell. 2012 Dec 21;151(7):1457-73. doi: 1

Insert

YAP1 (YAP1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

S61A, S109A, S127A, S128A, S131A, S163A, S164A, S…

Promoter

CMV

Available Since

March 5, 2013

Availability

Academic Institutions and Nonprofits only

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pTP396

Plasmid

#149336

Purpose

Bacterial expression of His14-Biotin_acceptor_peptide-SUMOEu1-anti-GFP_nanobody

Depositor

Rebecca Voorhees

Article

Pleiner et al Science. 2020 May 21. pii: science.abb50

Insert

anti-GFP nanobody

Tags

Biotin acceptor peptide (Avi-tag), His14, and SUM…

Expression

Bacterial

Available Since

July 1, 2020

Availability

Academic Institutions and Nonprofits only

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pARE-lux

Plasmid

#11768

Depositor

Joan Massague, Jeff Wrana

Article

Wrana et al Cell. 1992 Dec 11. 71(6):1003-14.

Insert

ARE

Use

Luciferase

Expression

Mammalian

Available Since

May 12, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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pRK7-FLAG-TSC2

Plasmid

#8996

Depositor

John Blenis

Article

Tee et al Proc Natl Acad Sci U S A. 2002 Oct 15. 9

Insert

TSC2 (TSC2 Human)

Tags

FLAG

Expression

Mammalian

Available Since

Oct. 11, 2005

Availability

Academic Institutions and Nonprofits only

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pJRH029

Plasmid

#171060

Purpose

Plasmid for expressing Gag fused to spyCas9-3x FLAG-2x SV40 NLS with the SQNYPIVQ HIV-1 cleavage site in between

Depositor

Jennifer Doudna

Article

Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

Insert

Gag-spyCas9

Use

CRISPR and Lentiviral

Tags

2x SV40 NLS and 3x FLAG tag

Expression

Mammalian

Promoter

CAG

Available Since

June 30, 2021

Availability

Academic Institutions and Nonprofits only

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pDESTmycIGF2BP3

Plasmid

#19879

Depositor

Thomas Tuschl

Article

Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

Insert

Insulin-like growth factor 2 mRNA binding protein 3 (IGF2BP3 Human)

Tags

MYC

Expression

Mammalian

Available Since

Dec. 31, 2008

Availability

Academic Institutions and Nonprofits only

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Flag-HA-VCPIP1

Plasmid

#22592

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

VCPIP1 (VCPIP1 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available Since

Dec. 4, 2009

Availability

Academic Institutions and Nonprofits only

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pDONR223 SARS-CoV-2 NSP16

Plasmid

#141269

Purpose

Gateway-compatible Entry vector, with insert of NSP16 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

NSP16 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway Cloning

Mutation

Many synonymous changes due to codon optimization

Available Since

April 1, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pMXs-IP spGFP-ERGIC53

Plasmid

#38270

Depositor

Noboru Mizushima

Article

Itakura et al Autophagy. 2010 Aug;6(6):764-76.

Insert

ERGIC-53 (LMAN1 Human)

Use

Retroviral

Tags

EGFP with Calreticulin signal peptide at N-termin…

Expression

Mammalian

Mutation

deleted signal peptide (1-30 amino acids)

Available Since

Aug. 28, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-ACE2-GFP

Plasmid

#154962

Purpose

pcDNA3.1-ACE2-GFP

Depositor

Utpal Pajvani

Article

Bartolome et al Sci Rep. 2021 May 7;11(1):9803. doi: 10.

Insert

ACE2-GFP (ACE2 Human)

Tags

EGFP

Expression

Mammalian

Available Since

July 14, 2020

Availability

Academic Institutions and Nonprofits only

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pGP-AAV-CAG-FLEX-jGCaMP7s-WPRE

Plasmid

#104495

Purpose

AAV-mediated expression of ultrasensitive protein calcium sensor under the CAG promoter, Cre mediated flip-excision switch

Depositor

Douglas Kim, GENIE Project

Article

Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

Has Service

AAV PHP.eB, AAV Retrograde, and AAV1

Insert

jGCaMP7s

Use

AAV and Cre/Lox

Tags

T7 epitope, Xpress tag, 6xHis

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pGFP-Rab8A[T22N]

Plasmid

#24899

Depositor

Maxence Nachury

Article

Nachury et al Cell. 2007 Jun 15. 129(6):1201-13.

Insert

hRab8A[T22N]

Tags

GFP

Expression

Mammalian

Mutation

Changed Threonine 22 to Asparagine

Available Since

June 17, 2010

Availability

Academic Institutions and Nonprofits only

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pBS-HA::UPRT

Plasmid

#47110

Purpose

for subcloning UPRT into expression vectors

Depositor

Mike Cleary, Chris Doe

Article

Miller et al Nat Methods. 2009 Jun;6(6):439-41. doi:

Insert

uracil phosphoribosyltransferase

Tags

HA

Available Since

Oct. 15, 2013

Availability

Academic Institutions and Nonprofits only

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EGFP-Tpr

Plasmid

#35024

Depositor

Larry Gerace

Article

Frosst et al J Cell Biol. 2002 Feb 18;156(4):617-30.

Insert

Tpr (TPR Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 12, 2012

Availability

Academic Institutions and Nonprofits only

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Tol2-elavl3-H2B-GCaMP6s

Plasmid

#59530

Purpose

Expressed nuclear-localized GCaMP6-slow, a calcium indicator, in neurons in zebrafish larva. The plasmid is in Tol2 transposon backbone

Depositor

Misha Ahrens

Article

Freeman et al Nat Methods. 2014 Jul 27. doi: 10.1038/n

Inserts

elavl3 promoter (elavl3 Zebrafish)
H2B
GCaMP6s

Use

Transposable element

Available Since

Sept. 12, 2014

Availability

Academic Institutions and Nonprofits only

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Slug_pGL2

Plasmid

#31695

Depositor

Paul Wade

Article

Fujita et al Cell. 2003 Apr 18. 113(2):207-19.

Insert

Slug (SNAI2 Human)

Use

Luciferase

Expression

Mammalian

Available Since

Aug. 29, 2011

Availability

Academic Institutions and Nonprofits only

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pDAC439

Plasmid

#195237

Purpose

Mammalian expression of Csm complex from single promoter; RFP backbone (Used for RNA knockdown)

Depositor

Jennifer Doudna

Article

Colognori et al Nat Biotechnol. 2023 Jan 23. doi: 10.103

Insert

FLAG-NLS-Csm5-2A-FLAG-NLS-Csm4-2A-FLAG-NLS-Csm3-2A-FLAG-NLS-Csm2; RFP-2A-FLAG-NLS-Cas6-2A-FLAG-NLS-Csm1; crRNA

Expression

Mammalian

Available Since

Jan. 27, 2023

Availability

Academic Institutions and Nonprofits only

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Cas12f-GE ver4.1

Plasmid

#176544

Purpose

Expresses Cas12f-GE in mammalian cells

Depositor

Yong-Sam Kim

Article

Kim et al Nat Biotechnol. 2021 Sep 2. pii: 10.1038

Inserts

Cas12f-GE ver4.1
Cas12f-GE ver4.1

Expression

Mammalian

Promoter

U6 and chicken β-actin

Available Since

Oct. 19, 2021

Availability

Academic Institutions and Nonprofits only

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