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pB1H2w5-12-C81S

Plasmid

#18043

Depositor

Scot Wolfe

Article

Noyes et al Nucleic Acids Res. 2008 May;36(8):2547-6

Insert

HhaI C81S mutant

Expression

Bacterial

Mutation

Simply used as an insert between Kpn1 and Xba1

Available Since

June 20, 2008

Availability

Academic Institutions and Nonprofits only

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pMM0531

Plasmid

#128988

Purpose

Part plasmid for VP16-CIB1, Type 3 (coding sequence) part

Depositor

Megan McClean

Article

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

SV40NLS-VP16-CIB1

Use

Synthetic Biology

Available Since

Oct. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pMM0530

Plasmid

#128987

Purpose

Part plasmid for Zif268DBD-CRY2PHR, Type 3 (coding sequence) part

Depositor

Megan McClean

Article

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

SV40NLS-ZiF268DBD-CRY2PHR

Use

Synthetic Biology

Available Since

Oct. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pMM0528

Plasmid

#128985

Purpose

Part plasmid for pZF(3BS), Type 2 (promoter) part

Depositor

Megan McClean

Article

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

pZF(3BS)

Use

Synthetic Biology

Available Since

Oct. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/HisC 14-3-3 sigma

Plasmid

#11947

Depositor

Michael Yaffe

Article

Wilker et al J Biol Chem. 2005 May 13. 280(19):18891-

Insert

Stratifin (SFN Human)

Tags

His

Expression

Mammalian

Available Since

May 17, 2006

Availability

Academic Institutions and Nonprofits only
CMVTO-SEAP-26SFur-B2AD-tvmvs-KKMP

Plasmid

#179645

Purpose

TVMVP-inducible single TM SEAP RELEASE construct

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

SEAP RELEASE

Expression

Mammalian

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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CMVTO-GFP-26Sfur-3TM-hcvs-KKMP

Plasmid

#179642

Purpose

HCVP-inducible tri-transmembrane GFP RELEASE construct

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

GFP RELEASE

Expression

Mammalian

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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human K18

Plasmid

#14741

Depositor

M Bishr Omary

Article

Ku et al J Cell Biol. 1994 Oct . 127(1):161-71.

Insert

Keratin 18 (KRT18 Human)

Expression

Mammalian

Available Since

April 6, 2007

Availability

Academic Institutions and Nonprofits only
CMVTO-Fyn-nTVMVpmut-tevs-AP4-tevs-P3-cTVMVp

Plasmid

#179668

Purpose

Membrane-bound (Fyn) TEVP-inducible caged-cTVMVP

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

Caged cTVMVP

Expression

Mammalian

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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pMM0624

Plasmid

#128999

Purpose

pZF(3BS)-mRUBY2 cassette plasmid flanked by ConL2 and ConRE

Depositor

Megan McClean

Article

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

ConL2-pZF(BS)-mRUBY2-tADH1-ConRE AmpR-ColE1

Expression

Yeast

Available Since

Oct. 16, 2019

Availability

Academic Institutions and Nonprofits only

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OAH2

Plasmid

#64074

Purpose

Expression of O-acetyl-L-homoserine sulfhydrylase (OAH2) of Thermus thermophilus

Depositor

Vern Schramm

Article

Poulin et al J Org Chem. 2015 May 15;80(10):5344-7. d

Insert

O-acetyl-L-homoserine sulfhydrylase

Tags

none

Expression

Bacterial

Available Since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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pALM174

Plasmid

#82022

Purpose

an updated version of cLac94 (pACSA_PcptOO_YFP_PMB2__LacIWF). The homologous region upstream of the inducible system was fixed to add a stop codon for A1837. No difference in expression

Depositor

Brian Pfleger

Article

Markley et al ACS Synth Biol. 2015 May 15;4(5):595-603

Insert

YFP

Promoter

Pcptoo

Available Since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pDest17_Med15

Plasmid

#231887

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 Med15

Depositor

Aseem Ansari, Tanja Mittag

Article

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Med15

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Promoter

T7

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pBbA5k-AcrAB-AsLOV2*(543)

Plasmid

#210863

Purpose

IPTG inducible promoter (lacUV5) expressing AcrA and AcrB-AsLOV2*(543). AsLOV2*(543) is also described in the literature as LOVdeg.

Depositor

Mary Dunlop

Article

Tague et al Elife. 2024 Jan 25;12:RP87303. doi: 10.7

Inserts

acrA
acrB

Use

Synthetic Biology

Tags

AsLOV2*(543)

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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vYM013_phiC31-nTRE-Ins(high)-pEF1s(orig)-H2b-Citrine

Plasmid

#197681

Purpose

"Insert(high)-pEF1s(orig)" reporter plasmid

Depositor

Michael Elowitz

Article

Ma et al ACS Synth Biol. 2023 Aug 12. doi: 10.102

Insert

pEF1s-H2B-Citrine

Use

Synthetic Biology

Expression

Mammalian

Available Since

April 25, 2023

Availability

Academic Institutions and Nonprofits only

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vYM015_phiC31-nTRE-Ins(low)-pEF1s(orig)-H2b-Citrine

Plasmid

#197683

Purpose

"Insert(low)-pEF1s(orig)" reporter plasmid

Depositor

Michael Elowitz

Article

Ma et al ACS Synth Biol. 2023 Aug 12. doi: 10.102

Insert

pEF1s-H2B-Citrine

Use

Synthetic Biology

Expression

Mammalian

Available Since

April 25, 2023

Availability

Academic Institutions and Nonprofits only

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vYM142_phiC31-nTRE-pEF1s(high)(CC793)-H2B-citrine

Plasmid

#197685

Purpose

"pEF1s(high)" reporter plasmid, with CpG793 mutated to CC

Depositor

Michael Elowitz

Article

Ma et al ACS Synth Biol. 2023 Aug 12. doi: 10.102

Insert

pEF1s-H2B-Citrine

Use

Synthetic Biology

Expression

Mammalian

Available Since

April 25, 2023

Availability

Academic Institutions and Nonprofits only

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CMVTO-Lyn-nTVMVpmut-tevs-AP4-tevs-P3-cTVMVp

Plasmid

#179672

Purpose

Membrane-bound (Lyn) TEVP-inducible caged-cTVMVP

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

Caged cTVMVP

Expression

Mammalian

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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CMVTO-GFP-Kir2.1-HA-tevs-RXR-V2

Plasmid

#179705

Purpose

TEVP-inducible GFP-conjugated Kir2.1 RELEASE

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

Kir2.1

Expression

Mammalian

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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CMVTO-gfp-Kir2.1-HA-tevs-KKYL

Plasmid

#179706

Purpose

TEVP-inducible GFP-conjugated Kir2.1 RELEASE with KKXX motif

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

Kir2.1

Expression

Mammalian

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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