We narrowed to 8,637 results for: pcDNA3

1. pCDNA3_dCas7-11

   Plasmid

   #197605

   Purpose

   Expresses inactive Cas7-11 in mammalian cells

   Depositor

   Feng Zhang

   Article

   Strecker et al Science. 2022 Nov 25;378(6622):874-881.

   Insert

   dCas7-11
   

   Expression

   Mammalian

   Available Since

   Aug. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1_ADAR2DD_E488Q

   Plasmid

   #205598

   Purpose

   expresses human ADAR2 deaminase domain (E488Q hyperactive mutant) in mammalian cells

   Depositor

   Marc Friedlander

   Article

   Sekar et al Nat Biotechnol. 2023 Sep 21. doi: 10.103

   Insert

   ADAR2 deaminase domain (ADARB1 Human)
   

   Expression

   Mammalian

   Mutation

   E488Q hyperactive mutation

   Available Since

   Oct. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1_ADAR2DD_E488Q_Ago2

   Plasmid

   #205599

   Purpose

   expresses human ADAR2 deaminase domain (E488Q hyperactive mutant) fused with human Ago2 in mammalian cells

   Depositor

   Marc Friedlander

   Article

   Sekar et al Nat Biotechnol. 2023 Sep 21. doi: 10.103

   Insert

   hAgo2 (AGO2 Human)
   

   Tags

   ADAR2DD_E488Q (hyperactive mutant)

   Expression

   Mammalian

   Available Since

   Oct. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1_crGE

   Plasmid

   #172933

   Purpose

   Crowding FRET sensor with mCerulean3 and mCitrine for mammalian cell expression. Linker comprises 2 helices and 3 GSG domains.

   Depositor

   Arnold Boersma

   Article

   Boersma et al Nat Methods. 2015 Mar;12(3):227-9, 1 p f

   Insert

   CrowdingSensorGE
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDNA3.1_3XFlag

   Plasmid

   #182494

   Purpose

   Empty plasmid containing Flag tag for C-terminal tagging

   Depositor

   Paolo Comoglio

   Article

   Cerqua et al Life Sci Alliance. 2022 May 30;5(10). pi

   Insert

   3xFlag with 5 Glycolic spacer
   

   Available Since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1_FASN

   Plasmid

   #107138

   Purpose

   His tagged human FASN cloned in pcDNA3.1 vector for expression in mammalian cells

   Depositor

   Brijesh Kumar Singh

   Insert

   Fatty acid synthase (FASN Human)
   

   Tags

   His

   Expression

   Mammalian

   Available Since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDNA3.1_WiChR_TS_mScarlet_ER

   Plasmid

   #195190

   Purpose

   High efficient, K+ selective channelrhodopsin for the inhibition of excitable cells

   Depositor

   Johannes Vierock, Peter Hegemann

   Article

   Vierock et al Sci Adv. 2022 Nov 16:eadd7729.

   Insert

   Wobblia lunata inhibitory Channelrhodopsin
   

   Tags

   ER-export sequence, Kir2.1 trafficking motif, and…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1hSIRT3_H248Y_HA

   Plasmid

   #24492

   Depositor

   Eric Verdin

   Article

   Hirschey et al Nature. 2010 Mar 4. 464(7285):121-5.

   Insert

   Human Sirtuin 3 catalytically inactive mutant (SIRT3 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   H248Y

   Available Since

   April 23, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDNA3_cAMPFIRE-H

   Plasmid

   #182281

   Purpose

   Mammalian expression of the cAMP sensor cAMPFIRE-H under a CMV promotor.

   Depositor

   Haining Zhong

   Article

   Massengill et al Nat Methods. 2022 Nov;19(11):1461-1471.

   Insert

   cAMPFIRE-H (cAMP sensor)
   

   Expression

   Mammalian

   Available Since

   March 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1_RFP

    Plasmid

    #206177

    Purpose

    mammalian expression of RFP

    Depositor

    Christopher Goodnow

    Article

    Russell et al Proc Natl Acad Sci U S A. 2021 Aug 24;11

    Insert

    RFP
    

    Expression

    Mammalian

    Mutation

    NA

    Promoter

    CMV

    Available Since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1_hHNF4alpha

    Plasmid

    #206878

    Purpose

    The ORF of HNF4α (variant 2) cDNA amplified from the RT human liver RNA was cloned at the Bam HI/XbaI sites of the pcDNA3.1(+), the mammalian expression vector.

    Depositor

    Reza Zolfaghari, Catharine Ross

    Article

    Zolfaghari et al J Cell Biochem. 2014 Oct;115(10):1740-51

    Insert

    Human hepatocyte nuclear factor 4 alpha (HNF4A Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. LXRalpha_pcDNA3.1

    Plasmid

    #177621

    Purpose

    Full-length human LXR alpha cDNA sequence in pcDNA3.1+

    Depositor

    Thomas Burris

    Article

    Griffett et al ACS Chem Biol. 2022 Apr 13. doi: 10.1021

    Insert

    LXR alpha (NR1H3 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.3_CoV1_D28

    Plasmid

    #170447

    Purpose

    Express SARS-CoV-1 WT spike protein with a 28aa deletion on the C-terminal tail. Used for SARS1 pseudotyped virus production.

    Depositor

    David Nemazee

    Article

    Rogers et al Science. 2020 Aug 21;369(6506):956-963.

    Insert

    SARS-CoV-1 spike D28 (S SARS-CoV-1)
    

    Tags

    NA

    Expression

    Mammalian

    Mutation

    last 28aa deletion in c-terminal tail

    Promoter

    CMV

    Available Since

    May 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCDNA3.3_MERS_D12

    Plasmid

    #170448

    Purpose

    Express MERS WT spike protein with an 12aa deletion on the C-terminal tail. Used for MERS pseudotyped virus production.

    Depositor

    David Nemazee

    Article

    Rogers et al Science. 2020 Aug 21;369(6506):956-963.

    Insert

    MERS spike D12 (S MERS)
    

    Expression

    Mammalian

    Mutation

    last 12aa deletion in c-terminal tail

    Promoter

    CMV

    Available Since

    July 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.3_SARS2_omicron BA.2

    Plasmid

    #183700

    Purpose

    expressing SARS-CoV-2 BA.2 spike protein for pseudovirus production

    Depositor

    David Nemazee

    Article

    Dacon et al Science. 2022 Jul 12:eabq3773. doi: 10.1

    Insert

    Spike (BA.2) (S SARS-CoV-2)
    

    Use

    Lentiviral and Retroviral

    Expression

    Mammalian

    Mutation

    c-terminal 18 aa deletion

    Promoter

    CMV

    Available Since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDNA3_LuxSit_i_mTagBFP2

    Plasmid

    #194300

    Purpose

    Mammalian expression of the de novo luciferase, LuxSit-i

    Depositor

    Andy Yeh, David Baker

    Article

    Yeh et al Nature. 2023 Feb;614(7949):774-780. doi:

    Insert

    LuxSit-i
    

    Tags

    mTagBFP2

    Expression

    Mammalian

    Available Since

    March 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCDNA3_cAMPFIRE-L

    Plasmid

    #182279

    Purpose

    Mammalian expression of the cAMP sensor cAMPFIRE-L under a CMV promotor.

    Depositor

    Haining Zhong

    Article

    Massengill et al Nat Methods. 2022 Nov;19(11):1461-1471.

    Insert

    cAMPFIRE-L (cAMP sensor)
    

    Expression

    Mammalian

    Available Since

    March 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. ERroGFP_iE_pCDNA3

    Plasmid

    #47954

    Purpose

    Mammalian expression vector encoding for the ER targeted and FLAGM1 tagged redox probe roGFPiE (roGFP C147, E147b, C204)

    Depositor

    David Ron

    Article

    Avezov et al J Cell Biol. 2013 Apr 15;201(2):337-49.

    Insert

    ERroGFP
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    insertion E147B

    Available Since

    Oct. 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.3_SARS2_omicron_BA.1

    Plasmid

    #180375

    Purpose

    Pseudovirus plasmid expressing SARS-CoV-2 omicron BA.1 strain spike protein

    Depositor

    David Nemazee

    Article

    Dacon et al Science. 2022 Jul 12:eabq3773. doi: 10.1

    Insert

    Spike (omicron BA.1 variant) (S SARS-CoV-2)
    

    Use

    Lentiviral and Retroviral

    Expression

    Mammalian

    Mutation

    c terminal D18

    Promoter

    CMV

    Available Since

    Feb. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1_MMP-9-HA

    Plasmid

    #121172

    Purpose

    Expression of mouse MMP9

    Depositor

    M. Elizabeth Fini, Shinwu Jeong

    Article

    Jeong et al Am J Pathol. 2012 May;180(5):2028-39. do

    Insert

    MMP9 (matrix metallopeptidase 9) (Mmp9 Mouse)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    See depositor comments below

    Promoter

    CMV immediate/early

    Available Since

    March 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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